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pseudorabies virus
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  “pseudorabies virus”译为未确定词的双语例句
     The positive pseudorabies virus was 27 with positive rate(32.1)%,and positive pig farm was 12 with positive rate 33.3%.
     PRV阳性病料27份,阳性率32.1%,阳性场12个,场阳性率33.3%;
短句来源
     Study on Porcine Parvovirus and Pseudorabies Virus Recombinant Live Vaccine SA215 (B)
     猪细小病毒-伪狂犬病重组病毒活疫苗SA215(B)的研究
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     Study on Pseudorabies Virus Gene-deleted Vaccine, SA215 Strain
     伪狂犬病基因缺失疫苗SA215研究
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     Establishment and Application of PCR Method for Detection of Porcine Pseudorabies Virus
     猪伪狂犬病PCR检测方法的建立与应用
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     The Biological Characteristics of a Gene-deleted Pseudorabies Virus Vaccine Strain (SA215)
     伪狂犬病基因缺失疫苗株(SA215)生物学特性研究
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  相似匹配句对
     The Detection of Pseudorabies virus with PCR
     应用PCR技术检测伪狂犬病病毒
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     Research Progress in the Pseudorabies Virus-Vector
     猪伪狂犬病毒载体的研究进展
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     release of the virus.
     组装病毒病毒颗粒;
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     Virus"of Marriage
     捕杀婚姻的“病毒”
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  pseudorabies virus
Construction and immunogenicity of recombinant pseudorabies virus expressing the modified GP5m protein of porcine reproduction a
      
Pseudorabies virus (PRV), an alpha-herpesvirus, has been developed as a live viral vector for animal vaccines.
      
Tn7-mediated introduction of DNA into bacmid-cloned pseudorabies virus genome for rapid construction of recombinant viruses
      
The integrity of this circuitry has been demonstrated by retrograde tracing with pseudorabies virus.
      
Viral transneuronal tracing with pseudorabies virus (PRV) was used to test this hypothesis.
      
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Pseudorabies virus can infect chick embryo and reproduce there by way of passing through egg yolk, allantoic cavity, allanto chorion. After infection, various gross lesions appear. The chick embryo inoculated through egg yolk sac and allantoic carity dies in 48-72 hours. In our experiments we found that the toxicity of amniotic fluid (a. f.) of the infected embryo through egg yolk was the highest. The infective dose of 50% tissue culture (TCID50) to H. K. cells was up to 107/0.1ml, that of embryo 108/0.1ml...

Pseudorabies virus can infect chick embryo and reproduce there by way of passing through egg yolk, allantoic cavity, allanto chorion. After infection, various gross lesions appear. The chick embryo inoculated through egg yolk sac and allantoic carity dies in 48-72 hours. In our experiments we found that the toxicity of amniotic fluid (a. f.) of the infected embryo through egg yolk was the highest. The infective dose of 50% tissue culture (TCID50) to H. K. cells was up to 107/0.1ml, that of embryo 108/0.1ml and that of allantoic fluid 102/0.1ml. To the inoculated allantoic cavity,the TCID60 of allantoic and amniotic fluid was 105/0.1ml, and that of embryo was 103/0.1ml.The pathological .changes occured in fifteen different monolayer cells of H.K., M.K, etc and were mainly two types: one was a big fusion or atr-ophic round necrosis in monolayer kidney cells and the other was atrophic round necrois in the cells of chick embryo.After the growth and reproduction of the virus in H.K. cells, the virus titre in the culture fluid, cytoplasm and cell karyoplasm was estimated 108 TCID50/0.1ml.when the virus reproduces continuously from generation to generation, its titre was up to 108 TCID60/0.1ml. The time of the appearence of pathological changes of cells was only 3 hours in comparison of 24 hours before.

牛伪狂犬病病毒(闽A株)能通过鸡胚卵黄囊、尿囊腔、尿囊膜感染和繁殖,并出现不同程度的病变,经卵黄囊、尿囊腔接种的鸡胚,均于48~72小时死亡,死亡率达100%。经卵黄囊接种的鸡胚其羊水含毒量最高,对H.K细胞半数感染量(TCID_(50))达10~7/0.1ml,胚胎为10~6/0.1ml,尿囊液为10~2/0.1ml;经尿囊腔接种的鸡胚,其尿囊液及羊水均为10~5/0.1ml,胚胎为10~3/0.1ml。 该毒株对H.K、M.K等15种单层细胞均可引起细胞致病作用,其病变可分为二种类型:于单层肾细胞上形成聚融合和萎缩圆形坏死,在鸡胚等细胞上形成萎缩圆形坏死。 病毒在H.K细胞上生长繁殖后,经测定在上清液、细胞质、细胞核质中均含有10~6TCID50/0.1ml的毒价。 牛伪狂犬病病毒经H.K细胞连续传代后,对H.K细胞的毒力可达10~8TCID_(50)/0.1ml,出现细胞病变的时间由原来的24小时缩短为3小时。

The ultrastsucture and morphogenesis of Pseudorabies Virus growing in hamster kideny culture cells were examined by means of electron microscopy. The complete virus was spherical shaped measuring 150~175 nm in diameter. The nucleocapsid was 94~103 nm and a electron dense core was filled in. Usually the specific viroplast and the nucleocapsids developing at various stages can be observed in the nuclei of infected cells. Sometimes enveloped nucleocapsids can be seen near the inner nuclear membrane....

The ultrastsucture and morphogenesis of Pseudorabies Virus growing in hamster kideny culture cells were examined by means of electron microscopy. The complete virus was spherical shaped measuring 150~175 nm in diameter. The nucleocapsid was 94~103 nm and a electron dense core was filled in. Usually the specific viroplast and the nucleocapsids developing at various stages can be observed in the nuclei of infected cells. Sometimes enveloped nucleocapsids can be seen near the inner nuclear membrane. Mature viruses are probably gradually released by exocytosis. Alot of fibrous structure and increasing membranes appeared in cytoplasm of infected cells.

病毒为圆形或卵圆形,直径为150~170nm,核壳体直径为94~103nm,内部充满致密的核心,在宿主细胞核内,可以观察到特异的毒浆结构和处于不同装配阶段的核壳体,有时在核膜内侧还可以见到被有囊膜的成熟病毒粒子。在细胞质中常常也可以见到装配好的核壳体和成熟病毒,但后者仅存在于细胞质的空泡中,成熟的病毒可能是通过与吞噬作用相反的过程逐渐向细胞外释放。膜增生现象和大量的纤维结构在病变的细胞质中是十分明显的。

The heperimmune serum against Pseudorabies virus used in thistest was prepared from guinea pig and the precipitating antigen was obtained bymeans of acetic acid dialysis. The cross counter immuno-electrophoresis test indi-cated that there is a common antigen shared by the YN strain, the Min A strainand Soviet Union strain of Pseudorabies virus. The procedure for the diagnosis ofpseudorabies established in this test is preferable to others in our country.

应用豚鼠制备伪狂犬病病毒高免血清,运用醋酸透析法制备伪狂犬病病毒沉淀抗原,通过交叉对流免疫电泳试验,证明伪狂犬病病毒YN株与闽A株、苏联株之间有共同抗原。本试验建立的对流免疫电泳方法,可供我国开展伪狂犬病的诊断研究参考。

 
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