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   rabbit hemorrhagic disease 的翻译结果: 查询用时:0.195秒
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rabbit hemorrhagic disease
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  兔出血症
     Cell strains of Vero E6, Vero, Fek, RK_(13), LLC-MK_2, A_(549) and ZBS were inoculated with rabbit hemorrhagic disease virus(RHDV), then followed by blind passage culture.
     将兔出血症病毒分别接种于Vero E_6、Vero、RK_(13)、LLC—MK_2、A_(549)、ZBS细胞进行盲传培养。
短句来源
     The capsid protein (VP60 ) gene of Rabbit hemorrhagic disease virus (RHDV) isolate NJ85 was amplified and sequenced.
     从兔出血症病毒(Rabbit hemorrhagic disease virus,RHDV)中国早期流行株NJ85中成功地克隆出衣壳蛋白(VP60)基因。
短句来源
     Cloning and Identification for Capsid Protein VP60 gene of Rabbit Hemorrhagic Disease Virus
     兔出血症病毒衣壳蛋白VP60基因的克隆与鉴定
     Cloning and Sequence Analysis for Capsid Protein VP60 Gene of Rabbit Hemorrhagic Disease Virus
     兔出血症病毒衣壳蛋白VP60基因克隆与序列分析
短句来源
     The capsid protein gene (vp60) of Rabbit hemorrhagic disease virus (RHDV) isolate NJ85 was amplified and sequenced, vp60 gene of NJ85 was in size of 1740nt and encoding 579aa.
     用分子克隆技术从兔出血症病毒(RHDV)中国早期流行株NJ85中成功克隆出vp60基因,序列分析表明基因长度为1740nt,编码579aa。
短句来源
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  兔出血病
     Establishment and Initial Application of RT-PCR Test for Rabbit hemorrhagic disease virus
     兔出血病病毒RT-PCR检测方法的建立及初步应用
短句来源
  “rabbit hemorrhagic disease”译为未确定词的双语例句
     Analysis and Sequencing of the Capsid Protein Gene of Rabbit hemorrhagic disease virus (RHDV) JX/97 Strain
     兔病毒性出血症病毒JX/97株衣壳蛋白基因的序列测定与分析
短句来源
     The capsid protein gene of Rabbit hemorrhagic disease virus (RHDV) JX/97 strain was cloned and sequenced .
     对中国兔病毒性出血症病毒(Rabbithemorrhagicdiseasevirus,RHDV)JX/97株的衣壳蛋白基因进行了克隆和测序。
短句来源
     Study on the Morphology and Structure of the Virion of the Rabbit Hemorrhagic Disease Virus(RHDV)
     免出血症病毒的形态与结构的研究
短句来源
     Normal rabbits were immunized for 5 times with tissular inactivated vaccine , The antibody titer against rabbit hemorrhagic disease virus in serum increased more than 12log2. The collected serum were used to treat the rabbits with rabbit hemorrhagic disease, Resulting that most kept alive.
     以兔病毒性出血症病毒组织灭活疫苗免疫家兔 ,经五次免疫之后 ,抗体效价可达1 2 log2以上 ,采集兔血清 ,以此治疗攻毒兔 ,结果大部分兔都存活 ;
短句来源
     A Dot-immune-gold-silver staining (Dot-IGSS) assay was developed forthe detection of IgG antibody against rabbit hemorrhagic disease . Theoptimum working concentration of mouse anti-rabbit IgG was determined tobe 1:200 that of goat anti-mouse IgG labeled colloidal gold to be 1:20 and theoptimum time of silver staining to be 15 minutes.
     本研究首次成功地建立了检测兔病毒性出血症抗体IgG的斑点免疫金银染色法(Dot-IGSS),并确定了操作流程中鼠抗兔IgG的工作浓度为1∶200,胶体金标记的羊抗鼠IgG的最佳工作浓度为1∶20及银染色最适时间为15min。
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  rabbit hemorrhagic disease
Rabbit hemorrhagic disease virus poly(A) tail is not essential for the infectivity of the virus and can be restored in vivo
      
Phylogenetic analyses conducted on isolates of rabbit hemorrhagic disease virus (RHDV) from throughout the world have shown well-defined genogroups comprising representative strains of the virus and antigenic variants.
      
Molecular and antigenic characterization of rabbit hemorrhagic disease virus isolated in Cuba indicates a distinct antigenic sub
      
Rabbit hemorrhagic disease virus (RHDV) and European brown hare syndrome virus (EBHSV) are caliciviruses that produce severe symptoms and are lethal to rabbits and hares.
      
Folding of the rabbit hemorrhagic disease virus capsid protein and delineation of N-terminal domains dispensable for assembly
      
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Virus particles were found in the parenchymal viscera extracts from rabbits died of acute septicemic infection.The disease could be reproduced in animals by challenging with infected tissues, and the agent was again detected in visceral samples.It was affirmed that the disease is a viral infection, which has been named provisionally as"rabbit viral hemorrhagic disease"(RVHD), and the causal agent "rabbit hemorrhagic disease virus" ( RHDV ) . Preliminary identification showed that the virus particles were...

Virus particles were found in the parenchymal viscera extracts from rabbits died of acute septicemic infection.The disease could be reproduced in animals by challenging with infected tissues, and the agent was again detected in visceral samples.It was affirmed that the disease is a viral infection, which has been named provisionally as"rabbit viral hemorrhagic disease"(RVHD), and the causal agent "rabbit hemorrhagic disease virus" ( RHDV ) . Preliminary identification showed that the virus particles were roughly spherical , about 30-32nm in diameter, and without envelope. The buoyant density in CsCl was 1.32-1.34g/cm3.The nucleic acid of the virus probably consisted of a single unsegmented molecule of double-stranded RNA.Virus replication occ-ured in the cytoplasm.In qualitative tests in rabbit, the virus appeared to be resistant to ether, stable at pH 3 and 50℃.So far no cell culture system was found to be satisfactory for virus growth, including rabbit cells(kideny, liver,spleen,testis,and lung)and cell lines(MA-104,BHK-21 ,IBRS-2 and HeLa) . Almost all tissues of infected rabbits possessed hemaglutination activity, especially liver and lung.The hemagglutination titer of fresh liver sample reached as high as 10×2-18 unit/g ( wet weight) .Human type O was the most sensitive, while sheep RBC was agglutinated to moderate titer . Rabbits acquired protection by innoculation with formaldehyde inactivated tissue vaccine.High titer of HI antibody was produced in the immunized and recovered animals.From the data obtained, it was suggested that the virus is a new one, but further investigation should be carried out to clarify its taxonomy.

从我国新发生的一种家兔急性败血性传染病死兔内脏抽提物中,观察到典型的病毒粒子,回归兔可引起典型发病,再从病死兔内脏回收到同样病毒,证明该病系病毒性传染病,暂定名为“兔病毒性出血症”,病原暂定为“兔出血症病毒”。经初步鉴定,认为本病毒可能是一种首次发现的新病毒,属双股RNA病毒。但从病毒大小和核酸节段看,又不同于呼肠病毒科。最终归属正在进一步研究。

Rabbit Hemorrhagic Disease Virus (RHDV)was extracted and purified from infected rabbit liver by two phase distribution method and sucrose density gradient centrifugation. A UV absorption curve typical of viruses was obtained. OD260nm/OD280nm was 1.33.RHDV was negatively stained and examined under eletr0n microscope. Nonenveloped icosahedral particles with diameter ot 34—36nm were found. The capsid was composed of 32 capsomers with 5—6nm in width. Virus-like particales with 22nm in diameter was also...

Rabbit Hemorrhagic Disease Virus (RHDV)was extracted and purified from infected rabbit liver by two phase distribution method and sucrose density gradient centrifugation. A UV absorption curve typical of viruses was obtained. OD260nm/OD280nm was 1.33.RHDV was negatively stained and examined under eletr0n microscope. Nonenveloped icosahedral particles with diameter ot 34—36nm were found. The capsid was composed of 32 capsomers with 5—6nm in width. Virus-like particales with 22nm in diameter was also found.In CsCl solution, the Virus was banded at a buoyant density of 1.36—1.38g/cm~3. The sedimentation coefficient of the virus was 162S.Upon agarose gel electrophoresis (pH 8.3), the virus migrated toward the negative electrode.

从实验发病兔肝经过聚乙二醇葡聚糖硫酸钠(PEG-DS)二相分配法和蔗糖密度梯度离心提取和纯化兔出血症病毒。紫外测定呈典型的病毒紫外吸收曲线,260nm/280nm比值为1.33,1%的琼脂电泳检查呈一条带。负染电镜下观察,病毒无囊膜,二十面体立体对称,直径34—36nm,病毒子表面有直径5—6nm的壳粒32个。另外,电镜下还见到22nm左右的病毒颗粒。病毒在氯化铯中的浮密度为1.36—1.38g/cm~3,沉降系数为162S。琼脂糖电泳在pH8.3条件下,病毒子向阴极移动。

Hemagglutination characteristics of human erythrocytes by virus of rabbit hemorrhagic disease were systematically studied. It was shown that the optimal pH for hemagglutination ranged from 4.4 to 7.2.The virus could agglutinate only human erythrocytes in high titer. There was no obvious difference observed among red blood cells ( RBC ) of group A,B, AB and O. Hemagglutinating activity of the virus disappeared completely after incubation of the virus at 56℃ for 5 days or 80℃ for 15 minutes. Chloramin-T,...

Hemagglutination characteristics of human erythrocytes by virus of rabbit hemorrhagic disease were systematically studied. It was shown that the optimal pH for hemagglutination ranged from 4.4 to 7.2.The virus could agglutinate only human erythrocytes in high titer. There was no obvious difference observed among red blood cells ( RBC ) of group A,B, AB and O. Hemagglutinating activity of the virus disappeared completely after incubation of the virus at 56℃ for 5 days or 80℃ for 15 minutes. Chloramin-T, trypsin, sodium borohyride could eliminate the hemaggluting activity of the virus,while sodium metabissulfite, potassium periodate, RDE could not influence on it. Therefore, it was inferred that the hemagglutinin might be a glycoprotein on the virus capsid. The hemagglutinin receptors on the surface of human RBC were sensitive to chloroform and ether, while not to trypsin, potassium periodate, RDE and sodium borohydride. This finding suggested that the hemagglutinin receptors On human erythrocytes might be a lipid-cotai-ning component. The virus particles on the agglutinated RBC could be spontaneously eluted after an incubation at 37℃ for 24 hours. When RBC with absorbed virus were resuspended in borate buffer of pH 9.2 containing 0.28 M sucrose and incubated at 37℃ and 22℃ for 30 minutes, the virus particles could be eluted. The eluted RBC could be reag-glutinated by the virus.

本文系统地叙述了兔出血症病毒的血凝特性。试验结果表明,血凝的适宜pH为4.4—7.2。病毒只对人红细胞引起高滴度凝集,不同血型的红细胞无显著差别。血凝活性可耐56℃,5天才全部丧失;在80℃下15分钟完全破坏。氯胺T、胰蛋白酶、硼氢化钠可破坏病毒的血凝活性,偏重亚硫酸钠、过碘酸钾、受体破坏酶对它无影响。由此推断,血凝素可能是病毒衣壳上的一种糖蛋白。人红细胞表面受体对脂溶剂敏感,而胰蛋白酶、过碘酸钾、受体破坏酶、硼氢化钠不影响其活性,因而认为该受体是一种含脂类的物质。在37℃下24小时病毒能从红细胞上自然解脱;在pH9.2的硼酸缓冲液中,于37℃,22℃经30分钟也能解脱,解脱后的红细胞仍然能被病毒再凝集。

 
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