Histological analysis revealed that the viable chondrocytes and neochondrocytes at 12 weeks accounted for 62 0%±3.45%,65.89%±48% in the autograft group and 60.1%±3.98%,55.20%±7.57% in the allograft group.
Objective To investigate construction of enhanced green fluorescent protein (EGFP)labeled recombinant adenovirus containing Calcitonin Gene-related Peptide (CGRP); To establish an experimental rat thoracic-to-abdominal aorta transplantation model for study of allograft vasculopathy; to explore gene transfer calcitonin gene-related peptide suppresses the development of allograft vasculopathy and its mechanism.
目的构建增强型绿色荧光蛋白(Enhanced Green Fluorescent Protein, EGFP)标记的人降钙素基因相关肽(Calcitonin Gene-related Peptide,CGRP)重组腺病毒(Adenovirus, Ad)载体(Ad5-CGRP-EGFP),建立大鼠降主动脉-腹主动脉同种异体移植血管病变(Allograft vasculopathy,AV)模型,研究降钙素基因相关肽转基因对同种异体移植血管病变的防治作用,进一步探讨其作用机制。
Fifteen of the donors'lung were preserved for 6h under 4℃ using Euro- Collings solution; 14 for 22~25h using UW solution. Allograft left lung transplantation was carried out and the right pulmonary artery was occluded to test the function of implanted left lung.