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   genetic characterization 的翻译结果: 查询用时:0.204秒
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genetic characterization     
相关语句
  基因特征
     Genetic Characterization and Subtype Analysis of HIV-1 Strains in Two First Reported HIV Infective Cases in Qinghai Province
     青海省首次发现的2例HIV感染者HIV-1毒株的基因特征和亚型分析
短句来源
     Cloning and Genetic Characterization of L Fragment of the Genome of Foot-and-mouth Disease Virus O/NY00 Strain
     口蹄疫病毒O/NY00株基因组L片段的克隆及其基因特征
短句来源
     Genetic Characterization and Phylogenetic Analysis of HIV-1 Strains in Shandong province
     山东省HIV-1流行毒株的基因特征和系统树分析
短句来源
     This paper was to analyze the poliovirus serotype distribution in 12 serial stool specimens from a first immunodeficient vaccine-derived poliovirus(iVDPVs) case in China and the VP1 genetic characterization of the type Ⅲ iVDPVs was also studied.
     分析中国首例免疫缺陷疫苗衍生脊髓灰质炎病毒(immunodeficient vaccine-derived polioviruses,iVDPVs)急性弛缓性麻痹(acute flaccid paralysis,AFP)病例(实验室编号为9230)12份便标本中病毒血清型分布和分离物中Ⅲ型VP1区的基因特征
短句来源
     The Poliovirus Serotype Distribution in Serial Stool Samples from a First iVDPVs Case in China and the VP1 Genetic Characterization of Type Ⅲ iVDPVs
     中国首例iVDPVs病例粪便标本中病毒血清型分布和Ⅲ型iVDPVs VP1区基因特征
短句来源
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  基因特性
     Influenza surveillance and antigenic and genetic characterization of predominant strains in Wuhan area in 2003
     武汉市2003年流感监测及当前优势流行毒株的抗原性和基因特性分析
短句来源
     Antigenic and genetic characterization of A/Shenzhen/1/99(H3N2) virus
     A/深圳/1/99(H3N2)病毒抗原性及基因特性研究
短句来源
     Genetic characterization of the VP1 gene of the two strains of Coxsackie B_5 virus isolated from Zhejiang province in 2002
     浙江省2002年2例柯萨奇B_5病毒VP1区基因特性分析
短句来源
  遗传学特征
     Phenotype and genetic characterization of capsulated mucoid Streptococcus pneumoniae isolated from children
     黏液型肺炎链球菌的表型和遗传学特征
短句来源
     Phenotypic and genetic characterization of patients with features of “nonclassic”forms of cystic fibrosis
     非典型囊性纤维化患者的表现型和遗传学特征
短句来源
     Clinical and genetic characterization of Chinese hereditary nonpolyposis colorectal cancer families
     中国人遗传性非息肉病性结直肠癌临床及遗传学特征分析
短句来源
  遗传鉴定
     The Establishment of Multiplex - CAPS Technique and Its Application for Genetic Characterization in Tomato
     Multiplex-CAPS技术及其在番茄遗传鉴定中的应用
短句来源
     Construction and Transformation of Antisense Expression Vector Harboring Puroindoines Genes and Genetic Characterization of Transgenic Wheats with Over-expression Vector
     Puroindoines基因反义载体构建和转化及过量表达载体转基因小麦的遗传鉴定
短句来源

 

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      genetic characterization
    Molecular genetic characterization of H5N1 influenza virus strains isolated from poultry in the Kurgan region in 2005
          
    Molecular genetic characterization of the yeast Lachancea kluyveri
          
    Genetic Characterization of Rye Accessions with Regard to Leaf Rust Resistance
          
    Molecular genetic characterization of avian Chlamydophila psittaci isolates
          
    Morphologic and genetic characterization of Sarcocystis sp.
          
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    Superoxide dismutase(SOD) and its inhibitor,diethyldithiocarbamate(DDC),were used to increase and decrease respectively the SOD activity of A549 lung cancer cells in vitro so as to observe the relationship between SOD activity and the karyotype,aberration of chromosome,the ratio of S phase cells and proliferation index(PI).It was found that the cells cultured in the medium with SOD expressed a reduction of the ratio of Sphase cells(from 63.4 on the first day to 38.0 on the fifth day),reduction of PI(from 0.66...

    Superoxide dismutase(SOD) and its inhibitor,diethyldithiocarbamate(DDC),were used to increase and decrease respectively the SOD activity of A549 lung cancer cells in vitro so as to observe the relationship between SOD activity and the karyotype,aberration of chromosome,the ratio of S phase cells and proliferation index(PI).It was found that the cells cultured in the medium with SOD expressed a reduction of the ratio of Sphase cells(from 63.4 on the first day to 38.0 on the fifth day),reduction of PI(from 0.66 to 0.44) and reduction of chromosome aberration and karyotype(from 56-60 in the control group to 40-55 in the SOD group.At the same time,an increase of chromosome aberration and toxic damage was observed in the cells of the DDC group.The result suggests that the increase of SOD activity might partly improve the transformation of genetic characterizations for human lung cancer cells from malignance to nonmalignance,and in case the activity is decreased the toxic damage of the cells might become serious.

    用SOD及其活性抑制剂分别提高和降低体外培养的A549肺癌细胞内的SOD活性,以达到改变细胞内自由基浓度的目的,动态地观察了SOD活性与肺癌细胞的染色体核型、畸变率、S期细胞比率和细胞增殖指数变化之间的关系。结果,受SOD处理的细胞其S期细胞比率由第1天的63.4下降到第5天的38.0,增殖指数由第1天的0.66下降到第5天的0.44;培养后第16天的细胞染色体畸变率明显减少,染色体的干系范围由对照组的56~60条下降到40~55条。而受SOD活性抑制剂处理的细胞的染色体畸变率明显增高,并表现出一定的毒性变化。结果提示,在细胞生物学水平上,提高细胞内SOD活性可以部分地促进人肺癌细胞的某些遗传表型向正常方面转化,而降低SOD活性则可增加细胞毒性损害作用。

    Based on the review of the application development of genetic markersfor genetic characterization of plant pathogenic fungi,the application of DNA finger-printing in population genetic analysis of plant pathogenic fungi is comprehensively re-ported with the focus on repetitive sequences based DNA markers,and the impact ofpopulation genetics on plant pathology is briefly discussed as well.

    在总结植物病原真菌遗传标记的应用发展过程基础上,着重以重复序列为基础的DNA分子标记的应用,综述了近年来DNA指纹分析在植物病原真菌群体遗传分析中的应用。还简要讨论了群体遗传分析对植物病理学的深远影响

    Chinese reference isolates of predominant races of Puccinia striiformis f.sp. tritici, the causal agent of wheat stripe rust disease, were examined for genetic variation with the random amplified polymorphic DNA (RAPD) assay. Random decamer primers were used to amplify DNA isolated from 11 reference isolates of the 11 predominant races ,CY17,CY19,CY21,CY22,CY23, CY25,CY26,CY27,CY28,CY29,and Shuiyuan 11-1,respectively,and 5 isolates sampled form geographically separated regions belonging to two newly identified...

    Chinese reference isolates of predominant races of Puccinia striiformis f.sp. tritici, the causal agent of wheat stripe rust disease, were examined for genetic variation with the random amplified polymorphic DNA (RAPD) assay. Random decamer primers were used to amplify DNA isolated from 11 reference isolates of the 11 predominant races ,CY17,CY19,CY21,CY22,CY23, CY25,CY26,CY27,CY28,CY29,and Shuiyuan 11-1,respectively,and 5 isolates sampled form geographically separated regions belonging to two newly identified pathotypes (races),CY30 and CY31.of 114 RAPD loci amplified with 17 primers,75.4% were polymorphic. Genetic relatedness among the 16 reference isolates was determined by hierarchical cluster analysis of 58 selected RAPD loci generated by 17 random decamer primers. A lack of correlation between the virulence and RAPD dissimilarity matrices was observed, indicating the independence of DNA polymorphism from virulence diversity. DNA polymorphism was detected among isolates of different as well as of single races. RAPD patterns generated by some primers enabled us to resolve distinctly all the isolates tested. Compared with other attempts to develop sufficient markers for genetic analysis of P. striiformis f. sp. tritici, RAPD procedure is technically simple, rapid, and reproducible, and has potential application for stringent genetic characterization of the population structure of this obligata pathogen.

    用随机扩增多态性DNA(RandomamplifiedpolymorphicDNA,RAPDR)技术对中国小麦条锈菌(Pucciniastriiformisf.sp.tritici)流行小种的11个模式分离系(CY17,CY19,CY21,CY22,CY23,CY25,CY26,CY27,CY28,CY29,水源11-1)以及5个分属于两个新发现小种CY30和CY31的分离系进行了基因组DNA多态性分析.用17个10-核苷酸随机引物共获得114个RAPD标记,其中75.4%表现多态性.选取其中的58个RAPD标记通过系统聚类分析确定了供试分离系间的亲缘关系,并与以毒性标记为基础确定的分离系间的演化关系进行了比较.结果表明,DNA多态性与毒性多态性之间没有相关性.利用RAPD标记检测到了小种间以及小种内的遗传变异,有些引物扩增到了分离系特异的RAPD特征图谱。与其它基因组多态性分析技术相比,RAPD分析可为小麦条锈菌的遗传分析提供大量的分子标记,且具有技术操作简单、快速、安全以及仅需微量的模板DNA等优点,对该活体营养病菌的群体遗传结构分析极具潜力.

     
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