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   genetic characterization 在 预防医学与卫生学 分类中 的翻译结果: 查询用时:0.008秒
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genetic characterization
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  基因特征
    The genetic characterization and molecular evolution of echovirus 30 during outbreaks of aseptic meningitis
    无菌性脑膜炎暴发中Echo30病毒的基因特征及分子进化分析
短句来源
    Objective To study the variation and evolution rules and analyze the genetic characterization of echovirus 30 during an outbreak of aseptic meningitis.
    目的了解引起无菌性脑膜炎暴发的Echo30病毒的基因特征,分析其遗传变异规律及进化来源。
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  “genetic characterization”译为未确定词的双语例句
    Genetic characterization of the viral sequences indicates that the illegal blood donors in Henan carry HIV-1 subtype B and HCV genotypes 1 and 2, whereas those intravenous drug users in Xinjiang and Yunnan carry HIV-1 subtype C and HCV genotypes 1, 3, and 4. Conclusions: Our findings show that the situation of HIV-1 and HCV co-infection in China is serious.
    性接触途径 :3.7%(5 / 136 )。 静脉注毒者 (云南和新疆 )HCV感染以 1,3,4亚型最多 ,而输血人群 (河南省 )感染的HCV以 1,2亚型为主。
短句来源
    Objective To ascertain the genetic characterization and genotype of measles viruses isolated in Shanghai region, in 2005. Methods Measles virus was isolated from throat swab specimens collected from suspected measles cases and 450 bp fragment of C terminus of nucleprotein (N) gene was amplified by RT-PCR. Sequence analysis was conducted to ascertain the genotype and to compare the difference of nucleotide with other measles virus strain published in GenBank.
    目的了解2005年上海部分地区麻疹暴发流行株的基因型别和特性,加强麻疹病毒学监测。 方法采集暴发麻疹患者咽拭子标本分离病毒,通过RT-PCR扩增麻疹病毒N基因C末端450 bp核苷酸并进行序列测定,与GenBank中麻疹病毒各基因型参考株及国内其他地区的麻疹分离株进行基因比较。
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  genetic characterization
Molecular genetic characterization of H5N1 influenza virus strains isolated from poultry in the Kurgan region in 2005
      
Molecular genetic characterization of the yeast Lachancea kluyveri
      
Genetic Characterization of Rye Accessions with Regard to Leaf Rust Resistance
      
Molecular genetic characterization of avian Chlamydophila psittaci isolates
      
Morphologic and genetic characterization of Sarcocystis sp.
      
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Random amplified polymorphic DNA bacterial fingerprinting method was used for the genotype taxon in 26 strains of phage type M1 of salmonella typhi (S.typhi) isolated from different areas in Jiangsu Province during 1988-1997. The results showed that all isolates were divided into seven genotypes. The 14 isolates from 1988 to 1991 were classified into seven genotypes. It was suggested that the DNA polymorphism of isolates attributed to the epidemic of typhi in Jiangsu Province in the end of 80's and the beginning...

Random amplified polymorphic DNA bacterial fingerprinting method was used for the genotype taxon in 26 strains of phage type M1 of salmonella typhi (S.typhi) isolated from different areas in Jiangsu Province during 1988-1997. The results showed that all isolates were divided into seven genotypes. The 14 isolates from 1988 to 1991 were classified into seven genotypes. It was suggested that the DNA polymorphism of isolates attributed to the epidemic of typhi in Jiangsu Province in the end of 80's and the beginning of 90's. After 1991, the 91.76% (11/12) of the isolates were in the same group. The incidence of typhi were decreased in the same time. Random amplified polymorphic DNA fingerprinting method was a rapid and powerful tool for the genetic characterization of S.typhi.

选 择3 组随 机引 物 利 用 随机 扩 增 多 态 性 D N A 技术 ,分 析 了 江 苏 省不 同 年 代( 1988 ~199 7 年) 、不同 地区分 离到的26 株 M1 型伤 寒沙门 氏菌 的分 子流 行病 学特 征。综 合3 组引 物 的扩增结果分 析,26 株菌株 呈7 个基 因图谱型 。其中从 1988 ~1991 年 分离 到的 14 株菌 表现 为7 个基因型。伤 寒沙门 氏菌在长 期的进 化过程中 ,由于产 生了许多 新的基 因型,造成 了80 年 代末和 90 年代初江 苏省大范 围的伤 寒高强度 流行。1992 年 后伤寒菌 的基因 型趋 于同 一化, 且各 地也 呈高 度一致性,主 要以1 个 基因型 为主, 占9176 % ( 11/12) ,伤 寒疫情 也逐 步呈下 降趋 势。结果 显示随 机扩增多态性 D N A 技术是一项快速、分辨力高的 基因分型手段,可广泛用于微生物的分子流行病学 研究。

Objective: To investigate the prevalence and subtypes of HIV-1 and HCV in different groups of HIV-1 infected patients in China. Methods:HIV infection was detected using ELISA and confirmed by Western blot. HIV-1 viral load was measured by branched-DNA (bDNA) method. CD4 and CD8 cells count was measured by FACS. Anti-HCV was detected by ELISA method, and HCV genotypes were tested using real time PCR. Results:Of 239 HIV-1 infected patients, 56.9% (136/239) were HCV antibody positive. The majority of HCV co-infection...

Objective: To investigate the prevalence and subtypes of HIV-1 and HCV in different groups of HIV-1 infected patients in China. Methods:HIV infection was detected using ELISA and confirmed by Western blot. HIV-1 viral load was measured by branched-DNA (bDNA) method. CD4 and CD8 cells count was measured by FACS. Anti-HCV was detected by ELISA method, and HCV genotypes were tested using real time PCR. Results:Of 239 HIV-1 infected patients, 56.9% (136/239) were HCV antibody positive. The majority of HCV co-infection is associated with intravenous drug using (42.7%) and illegal blood donation (53.7%). HCV co-infection through sexual contact, however, is approximately 3.7%. Genetic characterization of the viral sequences indicates that the illegal blood donors in Henan carry HIV-1 subtype B and HCV genotypes 1 and 2, whereas those intravenous drug users in Xinjiang and Yunnan carry HIV-1 subtype C and HCV genotypes 1, 3, and 4. Conclusions: Our findings show that the situation of HIV-1 and HCV co-infection in China is serious. HCV genotype 1 is the dominant subtype in China.

目的 :调查我国不同地区、通过不同传播途径感染人类获得性免疫缺陷病毒I型 (HIV 1)患者中丙型肝炎病毒(HCV)的流行情况及不同亚型的分布。方法 :采用酶联免疫吸附试验 (ELISA)检测抗 HIV 1并以蛋白印迹试验 (Westernblot,WB)进行确认。采用DNA分支放大 (bDNA)技术检测HIV 1病毒载量 ,采用荧光抗体流式细胞检测技术 (FACs)作CD4和CD8细胞计数。抗 HCV检测采用ELISA方法。HCV基因亚型的测定采用实时 (real time)聚合酶链反应 (PCR)方法。结果 :共检测了 2 39例HIV 1感染者 ,抗 HCV阳性率为 5 6 .9%(136 / 2 39) ,其中经不同传播途径感染HCV的阳性率分别为 :静脉注毒 :42 .7%(5 8/ 136 ) ;经血液 :5 3.7%(73/ 136 ) ;性接触途径 :3.7%(5 / 136 )。静脉注毒者 (云南和新疆 )HCV感染以 1,3,4亚型最多 ,而输血人群 (河南省 )感染的HCV以 1,2亚型为主。结论 :HIV 1感染者中存在HCV混合感染 ,我国HCV基因亚型以 1型为主。

Genetic characterization of 156 measles viruses isolated from throat swabs or urine specimens of measles patients using B95a cell line in nineteen provinces of the People's Republic of China during 1995-2002 was studied.The sequence analysis of 450 nucleotides of COOH-terminal of nucleoprotein(N)genes of 156 isolates indicated that all of the Chinese viruses were members of H1 genotype except Hunan95-6 and Henan99-1,that was same with Chinese measles vaccine strain S191,and belonged to genotype A.These...

Genetic characterization of 156 measles viruses isolated from throat swabs or urine specimens of measles patients using B95a cell line in nineteen provinces of the People's Republic of China during 1995-2002 was studied.The sequence analysis of 450 nucleotides of COOH-terminal of nucleoprotein(N)genes of 156 isolates indicated that all of the Chinese viruses were members of H1 genotype except Hunan95-6 and Henan99-1,that was same with Chinese measles vaccine strain S191,and belonged to genotype A.These Chinese measles viruses of H1 genotype were more diverse as compared to the other genotypes and differed from genotype A(Edmonston)at 6.00%~7.33%,from genotype H2(China94-1)at 5.11%~7.56% and from other 17 genotypes as much as 11.56% in the COOH-terminal of the N gene.The Chinese measles virus H1 genotype is the most genetically divergent within single genotype described so far.The sequencing data suggest that H1 genotype of 154 Chinese measles viruses can further be divided into two small subgroups,the H1a and the H1b,except Shanxi100-6 and Hunan-23.The H1a subgroup differed from H1b by 2.00%~4.00% at the nucleotide level in the COOH-terminal of the N gene.It is helpful to develop measles virus surveillance and set up measles virus strain bank and virus gene bank to accelerate measles control and eliminate measles in future.

为确定现阶段中国流行的麻疹野病毒本土基因型别 ,在 1995~ 2 0 0 2年用EB病毒转化的狨猴淋巴母细胞(B95a细胞 )从河南、山东、安徽、上海等 19个省 (自治区、直辖市 ,下同 )的麻疹爆发和散发病人的咽喉拭子或尿液标本中分离到麻疹病毒 15 6株。用逆转录 聚合酶链反应 (RT -PCR)从 15 6株麻疹病毒中扩增出核蛋白 (N)基因碳末端 4 5 0个核苷酸片段。通过对扩增产物的核苷酸序列测定和分析证明 ,15 4株为麻疹病毒H1基因型 ;2株属于A基因型 ,为沪191疫苗株。由此证实 ,H1基因型已在中国广泛流行 ,为中国麻疹病毒的优势基因型。H1是麻疹病毒型内变异最大的基因型。这 15 4株H1基因型麻疹病毒 ,除外Shanxi0 0 - 6和Hunan95 - 2 3株 ,可进一步划分为H1a和H1b两个亚组。H1a的 4 5 0个核苷酸和H1b的差异在 2 0 0 %~ 4 0 0 %。H1基因型的 4 5 0个核苷酸和A基因型代表株Edmonston的基因差异在 6 0 0 %~ 7 33% ;与H2基因型代表株China94 - 1的基因差异在 5 11%~7 5 6...

为确定现阶段中国流行的麻疹野病毒本土基因型别 ,在 1995~ 2 0 0 2年用EB病毒转化的狨猴淋巴母细胞(B95a细胞 )从河南、山东、安徽、上海等 19个省 (自治区、直辖市 ,下同 )的麻疹爆发和散发病人的咽喉拭子或尿液标本中分离到麻疹病毒 15 6株。用逆转录 聚合酶链反应 (RT -PCR)从 15 6株麻疹病毒中扩增出核蛋白 (N)基因碳末端 4 5 0个核苷酸片段。通过对扩增产物的核苷酸序列测定和分析证明 ,15 4株为麻疹病毒H1基因型 ;2株属于A基因型 ,为沪191疫苗株。由此证实 ,H1基因型已在中国广泛流行 ,为中国麻疹病毒的优势基因型。H1是麻疹病毒型内变异最大的基因型。这 15 4株H1基因型麻疹病毒 ,除外Shanxi0 0 - 6和Hunan95 - 2 3株 ,可进一步划分为H1a和H1b两个亚组。H1a的 4 5 0个核苷酸和H1b的差异在 2 0 0 %~ 4 0 0 %。H1基因型的 4 5 0个核苷酸和A基因型代表株Edmonston的基因差异在 6 0 0 %~ 7 33% ;与H2基因型代表株China94 - 1的基因差异在 5 11%~7 5 6 % ;与其它 17个基因型代表株的最大差异在 11 5 6 %。在中国开展麻疹病毒监测和建立麻疹病毒毒株库及基因数据库 ,对加速麻疹控制及未来消除麻疹都十分必要。

 
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