助手标题  
全文文献 工具书 数字 学术定义 翻译助手 学术趋势 更多
查询帮助
意见反馈
   genetic characterization 在 基础医学 分类中 的翻译结果: 查询用时:0.177秒
图标索引 在分类学科中查询
所有学科
基础医学
生物学
感染性疾病及传染病
畜牧与动物医学
预防医学与卫生学
临床医学
更多类别查询

图标索引 历史查询
 

genetic characterization
相关语句
  基因特征
    Genetic Characterization and Phylogenetic Analysis of HIV-1 Strains in Shandong province
    山东省HIV-1流行毒株的基因特征和系统树分析
短句来源
    Genetic Characterization and Subtype Analysis of HIV-1 Strains in Two First Reported HIV Infective Cases in Qinghai Province
    青海省首次发现的2例HIV感染者HIV-1毒株的基因特征和亚型分析
短句来源
    The Genetic Characterization of Echovirus 13 Isolated in China
    肠道病毒ECHO_(13)中国分离株的基因特征
短句来源
    The Poliovirus Serotype Distribution in Serial Stool Samples from a First iVDPVs Case in China and the VP1 Genetic Characterization of Type Ⅲ iVDPVs
    中国首例iVDPVs病例粪便标本中病毒血清型分布和Ⅲ型iVDPVs VP1区基因特征
短句来源
    Objective To analyse genetic characterization and subtype of HIV 1 strains in two first reported HIV infective cases in Qinghai Province.
    目的 分析青海省首次发现的HIV感染者HIV 1毒株的基因特征及亚型。
短句来源
更多       
  基因特性
    Antigenic and genetic characterization of A/Shenzhen/1/99(H3N2) virus
    A/深圳/1/99(H3N2)病毒抗原性及基因特性研究
短句来源
  遗传学特征
    Phenotype and genetic characterization of capsulated mucoid Streptococcus pneumoniae isolated from children
    黏液型肺炎链球菌的表型和遗传学特征
短句来源
  “genetic characterization”译为未确定词的双语例句
    The feature of influenza virus activity and genetic characterization of HA1 gene of influenza A H3N2 viruses isolated from 1994~1997 in Shenzhen
    1994~1997年深圳地区流感病毒活动及H3N2亚型毒株HA1基因演变特点
短句来源
    Genetic characterization of the non-structural protein NSP4 from epidemic strains of human rotavirus in China
    中国轮状病毒非结构蛋白NSP4基因变异特征的分析
短句来源
    Study on the antigenic and genetic characterization of variant of influenza virus H_3N_2
    H_3N_2流感病毒变异株抗原性的分子学研究
短句来源
    Objective To understand the feature of influenza virus activity and genetic characterization of HA1 gene of influenza A H3N2 viruses isolated in recently years in Shenzhen.
    目的 了解近几年流感病毒在深圳地区活动的特点及甲3(H3N2) 亚型毒株HA1 基因演变概况。
短句来源
    Methods Genetic characterization of 15 measles viruses isolated from 56 throat swabs or urine specimens of measles patients using B95_a cell line in Shaanxi Province was studied.
    方法在2000~2003年采集陕西省麻疹爆发和散发病人的咽喉拭子标本56份,用EB病毒转化的狨猴淋巴母细胞(B95a)分离到麻疹病毒15株。
短句来源
更多       
查询“genetic characterization”译词为用户自定义的双语例句

    我想查看译文中含有:的双语例句
例句
为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。
  genetic characterization
Molecular genetic characterization of H5N1 influenza virus strains isolated from poultry in the Kurgan region in 2005
      
Molecular genetic characterization of the yeast Lachancea kluyveri
      
Genetic Characterization of Rye Accessions with Regard to Leaf Rust Resistance
      
Molecular genetic characterization of avian Chlamydophila psittaci isolates
      
Morphologic and genetic characterization of Sarcocystis sp.
      
更多          


In order to investigate the DNA polymorphism of Cladosporium carrionii and Cladophialophora ajelloi, and to find the relationship of DNA patterns with morphology and geographic origin, the total DNA was extracted by using benzyl bromide method, and random amplification of polymorphic DNA(RAPD) assay was used to the study of genetic similarity(GS) of 21 isolates from five countries(19 of Cladosporium carrionii, 2 of Cladophialophora ajelloi). Results:(1)Of ten random primers used, three gave good reaction.(2)...

In order to investigate the DNA polymorphism of Cladosporium carrionii and Cladophialophora ajelloi, and to find the relationship of DNA patterns with morphology and geographic origin, the total DNA was extracted by using benzyl bromide method, and random amplification of polymorphic DNA(RAPD) assay was used to the study of genetic similarity(GS) of 21 isolates from five countries(19 of Cladosporium carrionii, 2 of Cladophialophora ajelloi). Results:(1)Of ten random primers used, three gave good reaction.(2) The DNA fragment patterns of 19 isolates of Cladosporium carrionii showed high genetic similarity, but a little difference in comparison with that of 2 isolates of Cladophialophora ajelloi. The RAPD patterns of two isolates showed high genetic similarity with most Chinese isolates of Cladosporium carrionii(GS∶85%).(3) The genetic similarity of Cladosporium carrionii are closely related to the geographic origin, the DNA patterns of isolates from the same country showed high similarity and located in the same group of dendrogram. RAPD assay can be used for the study of epidemiology and genetic characterization as a simple, rapid method.

研究卡氏枝孢霉与阿耶罗枝孢瓶霉的DNA多态性,了解两菌在DNA型别与形态学及菌种来源地域的相互关系。采用溴苯提取法提取DNA,以随机扩增DNA多态性方法对来自五个国家的21株分离株(19株卡氏枝孢霉,2株阿耶罗枝孢瓶霉)进行研究。结果:①10个随机引物筛选出3个具有较好DNA扩增的引物。②19株卡氏枝孢霉的DNA带型具有较大的遗传相似性,阿耶罗枝孢瓶霉的DNA带型与卡氏枝孢霉略有不同,但与中国株具有较大相似性(85%的遗传相似性)。③卡氏枝孢霉的遗传相似性与菌株的来源地域关系密切,同一国家的菌株具有相似的DNA带型,位于树状图的同一树组中。结论:随机扩增DNA多态性研究发现卡氏枝孢霉具有一定的种内差异,其DNA带型与菌株的来源地域关系密切,阿耶罗枝孢瓶霉的DNA带型与某些株卡氏枝孢霉更相似。本研究方法简便、快速,可用于流行病学及基因学特征的研究

Objective To investigate the DNA polymorphism of F pedrosoi and to find the relationship between DNA patterns and morphology in different geographic areas Methods The total DNA was extracted with benzyl bromide Random amplification of polymorphic DNA(RAPD) assay was used to study the genetic similarity (GS) of 32 isolates from seven countries (15 isolates from Japan, 3 isolates from China, 4 from Venezuela, 4 from Central America, 2 from Columbia, 2 from Mexico, 1 from Brazil and 1 from Thailand ) Results...

Objective To investigate the DNA polymorphism of F pedrosoi and to find the relationship between DNA patterns and morphology in different geographic areas Methods The total DNA was extracted with benzyl bromide Random amplification of polymorphic DNA(RAPD) assay was used to study the genetic similarity (GS) of 32 isolates from seven countries (15 isolates from Japan, 3 isolates from China, 4 from Venezuela, 4 from Central America, 2 from Columbia, 2 from Mexico, 1 from Brazil and 1 from Thailand ) Results ①Of ten random primers used, three (primers 1,7,8) gave good reactions The sequences of them were: primer 1: 5′ GAGCCCTCCA 3′, primer 7: 5′ CGGCCCCTGT 3′, primer 8: 5′ AGGTCACTGA 3′, ②The RAPD patterns of 32 isolates showed some degrees of variation ③The isolates from the same country showed high genetic similarities ④15 isolates from Japan could be divided into 2 types by RAPD analysis with primer 8 ⑤With the same primer,the common DNA fragment could be gotten from more than 90%isolates Conclusion There are some genetic variations between 32 isolates of F pedrosoi The genetic similarity of isolates is closely related to the geographic origin As a simple and rapid method, RAPD assay can be used for the study of epidemiology and genetic characterization of chromoblastomycosis

目的 进一步了解裴氏着色霉的基因学特征 ,采用溴苯提取法提取基因组DNA。方法 以随机扩增DNA多态性法对来源于 7个国家的 3 2株裴氏着色霉 (日本 15株 ,中国 3株 ,委内瑞拉 4株 ,中美洲 4株 ,哥伦比亚 2株 ,墨西哥 2株 ,巴西 1株 ,泰国 1株 )的DNA指纹图谱作了分析。结果 ①共选用 10个随机引物进行扩增 ,筛选出 3个具有较好DNA扩增片段的引物 ,即引物 1:5’ GAGCC CTCCA 3’ ,引物 7:5’ CGGCCCCTGT 3’ ,引物 8:5’ AGGTCACTGA 3’。② 3 2株裴氏着色霉的DNA带型不完全相同 ,具有一定的遗传变异性。③来源于同一国家的菌株具有较高的遗传相似性。④来源于日本的 15株菌株采用引物 8扩增后呈现两种DNA带型 ,I型 (有 3条主带 ) ,Ⅱ型 (有两条主带 ) ,其余国家菌株由于数量较少 ,DNA分型不明显。⑤同一引物扩增 ,90 %以上菌株可获共同的DNA片段。结论 随机扩增DNA多态性研究发现裴氏着色霉具有一定的种内差异 ,其DNA带型与菌株的来源地域关系密切 ,本研究方法简便快速 ,可用于着色芽生菌病的分子流行病学调...

目的 进一步了解裴氏着色霉的基因学特征 ,采用溴苯提取法提取基因组DNA。方法 以随机扩增DNA多态性法对来源于 7个国家的 3 2株裴氏着色霉 (日本 15株 ,中国 3株 ,委内瑞拉 4株 ,中美洲 4株 ,哥伦比亚 2株 ,墨西哥 2株 ,巴西 1株 ,泰国 1株 )的DNA指纹图谱作了分析。结果 ①共选用 10个随机引物进行扩增 ,筛选出 3个具有较好DNA扩增片段的引物 ,即引物 1:5’ GAGCC CTCCA 3’ ,引物 7:5’ CGGCCCCTGT 3’ ,引物 8:5’ AGGTCACTGA 3’。② 3 2株裴氏着色霉的DNA带型不完全相同 ,具有一定的遗传变异性。③来源于同一国家的菌株具有较高的遗传相似性。④来源于日本的 15株菌株采用引物 8扩增后呈现两种DNA带型 ,I型 (有 3条主带 ) ,Ⅱ型 (有两条主带 ) ,其余国家菌株由于数量较少 ,DNA分型不明显。⑤同一引物扩增 ,90 %以上菌株可获共同的DNA片段。结论 随机扩增DNA多态性研究发现裴氏着色霉具有一定的种内差异 ,其DNA带型与菌株的来源地域关系密切 ,本研究方法简便快速 ,可用于着色芽生菌病的分子流行病学调查及可能的基因学诊断中。

Objective To understand the feature of influenza virus activity and genetic characterization of HA1 gene of influenza A H3N2 viruses isolated in recently years in Shenzhen. Methods The specimens were inoculated into amniotic and allantoic cavities of the embryonated chicken eggs for viral isolation. The isolates were typed with HI test. Virion RNA extracted from allantoic fluid by Rneasy RNA extraction kit was used for RT PCR amplification. Afterward, the product of PCR was purified with purification...

Objective To understand the feature of influenza virus activity and genetic characterization of HA1 gene of influenza A H3N2 viruses isolated in recently years in Shenzhen. Methods The specimens were inoculated into amniotic and allantoic cavities of the embryonated chicken eggs for viral isolation. The isolates were typed with HI test. Virion RNA extracted from allantoic fluid by Rneasy RNA extraction kit was used for RT PCR amplification. Afterward, the product of PCR was purified with purification kit. RNA sequence analysis was performed by Sanger dideoxy mediated nucleotide chain termination method.Results Influenza A H3N2, H1N1 and B viruses were cocirculating in men in Shenzhen in recent years. However, the extent of their activities was not the same in a single year. The influenza B virus was the predominant strain in 1994 and 1997, but the predominant strain was H3N2 virus in 1996. The genetic analysis showed that the substitution of amino acids occured year by year and was mainly located at antigenic determinants or receptor binding site on HA1 domain of H3N2 viruses.Conclusion Influenza A H3N2, H1N1 and B viruses were still co circulating in men in Shenzhen in recent years, the predominant strain of influenza virus was different in different years. An increase of influenza A H3N2 virus activity in 1996 was due to the substitution of amino acids sequences on viral HA1 domain.

目的 了解近几年流感病毒在深圳地区活动的特点及甲3(H3N2) 亚型毒株HA1 基因演变概况。方法 病毒分离采用常规的鸡胚双腔接种,毒株检定用常量半加敏HI测定。新鲜收获含病毒粒的鸡胚尿囊液用来提取RNA,经逆转录合成cDNA,经聚合酶链反应(PCR) 扩增,产物纯化,采用双脱氧链末端终止法进行核苷酸序列测定。结果 近几年来深圳地区流感活动概况与全国情况相一致:在人群中仍同时流行H3N2,H1N1 亚型和乙型毒株,当甲型毒株活动减弱时,乙型毒株活动就增强,反之,甲型毒株增强时,乙型毒株就减弱。随着时间的推移,H3N2 亚型毒株HA1 基因不断地发生点突变,这种突变严重受人群免疫压力所影响,1996 年的毒株与1995 的毒株相比,不仅氨基酸替换点中多数是位于抗原决定簇区或受体结合部位上,并增加两个糖基化位点,故导致H3N2 毒株於1996 年活动明显增强。结论 近来在深圳地区人群中仍同时流行着H3N2,H1N1 亚型和乙型流感病毒。然而,不同年其优势毒株是不一样的。1996 年H3N2 毒株活动增强是由于其HA1 区氨基酸序列发生替换所造成。

 
<< 更多相关文摘    
图标索引 相关查询

 


 
CNKI小工具
在英文学术搜索中查有关genetic characterization的内容
在知识搜索中查有关genetic characterization的内容
在数字搜索中查有关genetic characterization的内容
在概念知识元中查有关genetic characterization的内容
在学术趋势中查有关genetic characterization的内容
 
 

CNKI主页设CNKI翻译助手为主页 | 收藏CNKI翻译助手 | 广告服务 | 英文学术搜索
版权图标  2008 CNKI-中国知网
京ICP证040431号 互联网出版许可证 新出网证(京)字008号
北京市公安局海淀分局 备案号:110 1081725
版权图标 2008中国知网(cnki) 中国学术期刊(光盘版)电子杂志社