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genetic characterization
相关语句
  基因特征
    Serotype Analysis of Enterovirus in an Outbreak and Genetic Characterization of Echovirus 30 Isolated from This Outbreak
    一起传染病暴发中肠道病毒血清型鉴定和ECHO30基因特征分析
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    Genetic Characterization of TypeⅡ Vaccine Mutant Polioviruses Isolated from Acute Flaccid Paralysis (AFP) Cases
    急性弛缓性麻痹病例中Ⅱ型脊髓灰质炎疫苗病毒变异株基因特征分析
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    Objective:To analyse the genetic characterization of the VP1 gene of Coxsackie B 5 virus causing meningitis isolated from Zhejiang province in 2002. Methods: The specimens of the cerebrospinal fluid (CSF) and feces were inoculated in Hep-2 and RD cell lines and two strains of Coxsackie B 5 virus were isolated.
    目的:研究2002年浙江省病毒性脑膜脑炎病原CoxB5 病毒VP1区的基因特征
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  “genetic characterization”译为未确定词的双语例句
    ObjectiveTo explore the genetic characterization and gen otype of measles viruses circulating in Shenzhen city during the year of 2000-20 02.MethodsExtracting RNA from throat swabs and urine of the measles patients,using RT-PCR amplification measles virus hemagglutinin gene o f 571bp.
    目的 探讨深圳市麻疹病毒的基因型别和特征。 方法 对深圳市专科医院 2 0 0 0~ 2 0 0 2年的 19例临床诊断为麻疹病例的咽拭子和尿液提取RNA ,通过RT PCR方法扩增麻疹病毒血凝素 (H)基因序列 ,并克隆到PMD18 Tvector进行序列测定 ;
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  genetic characterization
Molecular genetic characterization of H5N1 influenza virus strains isolated from poultry in the Kurgan region in 2005
      
Molecular genetic characterization of the yeast Lachancea kluyveri
      
Genetic Characterization of Rye Accessions with Regard to Leaf Rust Resistance
      
Molecular genetic characterization of avian Chlamydophila psittaci isolates
      
Morphologic and genetic characterization of Sarcocystis sp.
      
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ObjectiveTo explore the genetic characterization and gen otype of measles viruses circulating in Shenzhen city during the year of 2000-20 02.MethodsExtracting RNA from throat swabs and urine of the measles patients,using RT-PCR amplification measles virus hemagglutinin gene o f 571bp.PCR products were cloned into PMD18-T vector and the homogeneity of th e virus isolated were compared with the strains in genebank.ResultsH genes homogeneity was between the range of 96 2%-100% compared with other wild-type...

ObjectiveTo explore the genetic characterization and gen otype of measles viruses circulating in Shenzhen city during the year of 2000-20 02.MethodsExtracting RNA from throat swabs and urine of the measles patients,using RT-PCR amplification measles virus hemagglutinin gene o f 571bp.PCR products were cloned into PMD18-T vector and the homogeneity of th e virus isolated were compared with the strains in genebank.ResultsH genes homogeneity was between the range of 96 2%-100% compared with other wild-type viruses of H 1 genotype,the homogeneity was 96 4%-99 3%.ConclusionShenzhen circulating measles viruses were belo nged to the clade H 1.

目的 探讨深圳市麻疹病毒的基因型别和特征。方法 对深圳市专科医院 2 0 0 0~ 2 0 0 2年的 19例临床诊断为麻疹病例的咽拭子和尿液提取RNA ,通过RT PCR方法扩增麻疹病毒血凝素 (H)基因序列 ,并克隆到PMD18 Tvector进行序列测定 ;分析麻疹病毒和Genebank中麻病毒的各基因型代表株序列的同源性。结果  19株麻疹病毒H基因之间的同源性为 96 2 %~ 10 0 % ,和H1基因型代表株相比同源性为 96 4%~ 99 3 %。结论 深圳市麻疹流行株属于H1基因型

Objective:To analyse the genetic characterization of the VP1 gene of Coxsackie B 5 virus causing meningitis isolated from Zhejiang province in 2002. Methods: The specimens of the cerebrospinal fluid (CSF) and feces were inoculated in Hep-2 and RD cell lines and two strains of Coxsackie B 5 virus were isolated. Viral RNA was extracted and the VP1 gene was amplified by RT-PCR. The sequence of the VP1 gene was determined and compared by DNAMAN and Bioedit software. Results: The VP1 region of the two...

Objective:To analyse the genetic characterization of the VP1 gene of Coxsackie B 5 virus causing meningitis isolated from Zhejiang province in 2002. Methods: The specimens of the cerebrospinal fluid (CSF) and feces were inoculated in Hep-2 and RD cell lines and two strains of Coxsackie B 5 virus were isolated. Viral RNA was extracted and the VP1 gene was amplified by RT-PCR. The sequence of the VP1 gene was determined and compared by DNAMAN and Bioedit software. Results: The VP1 region of the two strains has 849 bp and the genetic and protein homogeneity was 98.7% and 100% respectively. Conclusion: The two strains of Coxsackie B 5 virus showed the same characterization and had the nearest relationship with the prototype of AF-114383 Cox B 5(Swend-16-1998) which mostly infected human central nervous system. But the genetic and protein homogeneity was 79.3%~81.5% and 96.82%~97.53% respectively when they were compared with the Coxsackie B 5 virus isolated from the west and the variation was obvious. The genetic and protein homogeneity was 98.5%~98.9% and 99.29%~99.65% respectively when they were compared with the Coxsackie B 5 virus MG-18-2001 and MG-39-2001 isolated from the meningitis patients′ feces samples in Mingguang city of Anhui province in 2001.So these four strains were on the same branch of the phylogenetic tree.[

目的:研究2002年浙江省病毒性脑膜脑炎病原CoxB5 病毒VP1区的基因特征。方法:用Hep-2和RD两种细粪便标本进行病毒分离,提取病毒RNA,再用RT-PCR扩增病毒VP1区基因片断,对纯化产物进行核苷酸序列测定,采用DNAMAN和Bioedit软件进行分析处理。结果:两株CoxB5 病毒的VP1区核苷酸长度均为849bp,二者核苷酸同源性为98 .7%,氨基酸同源性达100%。结论:浙江省两株CoxB5 病毒为同一性状毒株,它们的亲缘关系与侵犯中枢神经系统的CoxB5 的原型株AF-114383CoxB5 (Swend-16-1998)最为接近。但这两株CoxB5 毒株与欧美国家相比,核苷酸同源性仅为79 3% ~81. 5%,氨基酸同源性为96 .82~97. 53%,核苷酸序列有了18. 5% ~20 .7% 的变异,与国外报道的CoxB5 株之间存在一定的差异。这两株病毒与安徽明光市2001年从无菌性脑膜脑炎病人粪便中分离到的两株CoxB5 病毒MG-18-2001和MG-39-2001在同一小分枝上,核苷酸同源性达98 .5% ~98 .9%,氨基酸同源性达99 .29% ~99. 65%。

Objective:To analysis the relationship between the bacterium pathologinesis and the plasmid genetic characterization in Zhejiang province during 2003~2004. Methods: 78 isolates of S.paratyphi A from the patients in the broken-out spots and inpatients were analysed by plasmid profile, the resistance was also studied. Results: Among 78 isolates of S.paratyphi A analysed, 78(100%) isolates were resistant to Erythromycin; 77 isolates(98.72%) to Rifampicin and to Ceflazidime as well as to Streptomycin;...

Objective:To analysis the relationship between the bacterium pathologinesis and the plasmid genetic characterization in Zhejiang province during 2003~2004. Methods: 78 isolates of S.paratyphi A from the patients in the broken-out spots and inpatients were analysed by plasmid profile, the resistance was also studied. Results: Among 78 isolates of S.paratyphi A analysed, 78(100%) isolates were resistant to Erythromycin; 77 isolates(98.72%) to Rifampicin and to Ceflazidime as well as to Streptomycin; 76 isolates (97.44%) to Carboxy Benzylpenicinllin and to Sulfadiazine, SD.; 64 isolates (82.05%) to Doxycycline. Most of the isolates harbour one 20kb plasmid, and all of the isolates harbour one 2.2 kb plasmid. Conclusion: The finding indicate that S.paratyphi A were resistant to ETM、RFP、CAR、CV、SM、SD、DC and harbour one 20 kb plasmid and 2.2 kb plasmid;The multi-resistance of the isolates might continually serve the major reasons of the higher morbidity of typhoid fever in Zhejiang province recently.

目的:探讨浙江省2003、2004年不同地区分离的甲型副伤寒菌株质粒基因定位及与致病性的关系。方法:利用技术及电泳图谱方法,对浙江省2003、2004年暴发点病人和住院病人分离的部分甲型副伤寒菌株进行质粒图谱分析并做药敏试验。结果:分析发现选择的78株菌株中, 78株( 100% )对红霉素(ETM)耐药, 77株(98. 72% )对利福平(RFP)、链霉素(SM)及头孢拉定(CV)耐药, 76株(97 .44% )对羧苄青霉素(CAR)及磺胺(SD)耐药,64株(82 .05% )对强力霉素(DC)耐药;大多数菌株带有一20kb大小质粒,所有菌株均带有2 2kb大小的质粒。结论:甲型副伤寒菌对ETM、RFP、CAR、CV、SM、SD、DC耐药,并认为具有多重耐药性是浙江省甲型副伤寒发病率持续高发的主要原因。

 
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