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pcr product     
相关语句
  pcr产物
     The PCR product was a 418 bp fragment.
     PCR产物片段为418bp。
短句来源
     Specific PCR product was obtained from blood clot of 5 of the 22 IgM-positive patients.
     22例IgM阳性患者中,从5例患者的血凝块中扩增得到特异性PCR产物
短句来源
     The PCR product was linked to PMD18-T vector and transformed into E.
     将PCR产物连接到PMD18-T载体上,转化E.
短句来源
     Results: PCR product was 214 bp, some samples could't be amplicated by specific primers. They were deletional genotype of CYP2A6 gene(CYP2A6del/CYP2A6del).
     结果 :扩增的PCR产物大小为 2 14bp ,部分血样标本未获得PCR扩增产物 ,判为CYP2A6缺失基因型(CYP2A6del/CYP2A6del)。
短句来源
     The expressions of HIF-1αmRNA was remarkably increased at 2 hours of hypoxia, and reached the peak value at 8 hours,The PCR product HIF-1 to beta-actin(β-actin) signal ratio significantly increased from (3.85±1.98)%in the control group to(60.28±13.48)%(P< 0.01).
     而低氧培养则可显著提高胎肝间质细胞HIF-1α基因的mRNA及其蛋白水平。 HIF-1αmRNA在低氧培养2h时即显著增高,8h时达到高峰水平,PCR产物HIF-1与β-actin信号比从对照组(3.85±1.98)%增加到(60.28±13.48)%,差异有显著性意义(P<0.01);
短句来源
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  扩增产物
     Results: PCR product was 214 bp, some samples could't be amplicated by specific primers. They were deletional genotype of CYP2A6 gene(CYP2A6del/CYP2A6del).
     结果 :扩增的PCR产物大小为 2 14bp ,部分血样标本未获得PCR扩增产物 ,判为CYP2A6缺失基因型(CYP2A6del/CYP2A6del)。
短句来源
     PCR product of HPV18 E6/E7 gene were sequenced,reverse transcriptase(RT)-PCR amplification of HPV E6 and HPV E7 genes.
     对HPV18 E6/E7 PCR扩增产物片段进行测序;
短句来源
     The PCR product of the second primer(M2) was a specific fragment of INHBA gene(marked in INHBAⅡ) and the length of the fragment was 1100 bp.
     第二对引物(M2)扩增产物为1100bp 的beta-A 亚基基因特定片段(记为INHBAⅡ)。
短句来源
     By RT-PCR, the amounts of PCR product for bFGF AONs, SONs and DMEM control were 0. 33 μg, 0. 99 μg, 0. 85 μg.
     RT-PCR法检测结果显示,以2μg总RNA为模板,循环30次,bFGF反义寡核苷酸、bFGF正义寡核苷酸及营养液的扩增产物的量分别为0.33、0.99及0.85μg;
短句来源
     After the digestion of PCR product of NASBA with restriction enzyme SmaⅠ,two DNA bands were observed: 144bp and 359bp.
     扩增产物经聚合酶链反应(PCR)及内切酶SmaⅠ消化后产生预期大小的144bp和359bp的片段,第1次PCR产物经转录实验后与NASBA直接扩增的产物具有较好的同源性。
短句来源
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  pcr扩增产物
     Results: PCR product was 214 bp, some samples could't be amplicated by specific primers. They were deletional genotype of CYP2A6 gene(CYP2A6del/CYP2A6del).
     结果 :扩增的PCR产物大小为 2 14bp ,部分血样标本未获得PCR扩增产物 ,判为CYP2A6缺失基因型(CYP2A6del/CYP2A6del)。
短句来源
     PCR product of HPV18 E6/E7 gene were sequenced,reverse transcriptase(RT)-PCR amplification of HPV E6 and HPV E7 genes.
     对HPV18 E6/E7 PCR扩增产物片段进行测序;
短句来源
     We amply PCR by the primers of Q576R IL -4 receptor a allele . The PCR product is 209 bp.
     采用几一4 a受体 Q576R等位基因引物扩增 PCR,PCR扩增产物长度为209 hP。
短句来源
     The sequencing results confirmed that the PCR product was specific for DD3 mRNA.
     PCR扩增产物经测序分析证实为DD3mRNA特异性片段。
短句来源
     While primers c and b were the specific for the LMW-GS genes at Glu-B3 locus in wheat , the PCR product was about 1.45kb, including promoter and the whole CDS.
     引物b(5’-GTAGGCACCAACTCCGGTGC-3’)和c(5’-TCCTGAGAAGTGCATGACATG-3’)是谷蛋白Glu-B3位点LMW-GS基因特异引物,其PCR扩增产物约1500bp。
短句来源
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  聚合酶链反应产物
     RESULTS: ① Amplified results of PCR: The PCR product showed 536 bp and 300 bp in agarose gel electrophoresis respectively.
     结果:①聚合酶链反应扩增结果:聚合酶链反应产物在琼脂糖凝胶电泳中分别显示为536bp和300bp。
短句来源
     MAIN OUTCOME MEASURES: ① Result of PCR product clone.
     主要观察指标:①聚合酶链反应产物克隆结果。
短句来源
     ③ Result of construction of rAdV.RESULTS: ① Result of PCR product clone: Cloning constructed recombinant plasmid is named as pGEM-T/hBMP2, (3 015+1 213)bp in size.
     ③重组腺病毒的构建结果。 结果:①聚合酶链反应产物克隆结果:克隆构建的重组质粒命名为pGEM-T/hBMP2,大小为(3015+1213)bp。
短句来源
     The PCR product was partially digested into fragments of 347 bp and 252 bp by restriction enzyme Pst I.
     特发性1型糖尿病组中发现1例P239Q(CCG-CAG)杂合突变,该聚合酶链反应产物可被PstⅠ部分酶切,所切的2个片段大小分别为347和252bp,与预测相吻合。
短句来源
     Identification of Traditional Chinese Crude Drug Turtle Shell Using PCR Product Direct Sequencing Method
     用聚合酶链反应产物直接测序技术鉴定中药材鳖甲
短句来源

 

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  pcr product
Sequencing analysis of the aberrant fragments found a RT-PCR product missing exons 5-7 in one case of GC, and another product missing exons 4-7.
      
Direct sequencing of the PCR product demonstrated the complete identity of the predicted and real mRNA sequences.
      
The isolates from the two geographic areas differed in some physiological characteristics and in the PCR product profiles obtained with the microsatellite primers (CAC)5and (GACA)4.
      
From the DNA of the Pleistocene deposits, the PCR product was obtained only with bacterial primers.
      
Amplification with other bacterial species did not produce any PCR product detectable by electrophoresis.
      
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A Polymerase chain reaction has been developed to detect HBV DNA using FD DNA polymerase and domestic reagents. One fg of HBV DNA can be detected if the PCR product is electrophoresed and stained with ethidine bromide; 100ag if hybridized after Southern blotting. So the sensitivity of the test is identical with that reported abroad. Amplified number of DNA molecules of 205bp and 413 bp are derived from each set of primers respetively, and are consistent with the results calculated from the sequence. Besides,they...

A Polymerase chain reaction has been developed to detect HBV DNA using FD DNA polymerase and domestic reagents. One fg of HBV DNA can be detected if the PCR product is electrophoresed and stained with ethidine bromide; 100ag if hybridized after Southern blotting. So the sensitivity of the test is identical with that reported abroad. Amplified number of DNA molecules of 205bp and 413 bp are derived from each set of primers respetively, and are consistent with the results calculated from the sequence. Besides,they can be hybridized with the HBV probe. Those suggest the reaction is specific. It was attempted to test the sera from 20 patients with chronic active hepatitis. HBV DNA was detected in 17 cases while only in 6 with spot hybridization.

以国产DNA聚合酶和试剂建立聚合酶链反应,检测乙型肝炎病毒DNA,如以溴乙啶荧光显带可检出1fg HBV DNA,继以分子杂交则至少可检出0.1fg,灵敏性与国外报告相近。两组引物可分别获得208bp和415bp的扩增分子,与按序列计算的结果一致,并可与HBV DNA探针杂交,表明反应的特异性。试用于检测20例慢性活动性肝炎病人血清,检出HBV DNA17例,而斑点杂交仅可检出6例。

This paper focuses on the discussion of some related problems of the PCR amplification mechanism, operational techniques, systematic extensions and applications. Also, it introduces the "transformation and accumulation laws of PCR products" suggested by the author.

本文重点探讨了PCR放大机理、操作技术、系统扩展和应用研究诸方面的有关问题,并介绍了作者提出的“PCR放大产物的转变与积累规律”。

The polymerase chain reaction (PCR) is an in vitro DNA amplification procedure. With the participation of polymerase, dNTP and their primers, specific DNA of known sequences increased greatly in a few hours. Four strains (B, C, G, T) of E. histolytica DNA were used and PCR repeated for 30 cycles. The PCR products were put to gel eleotrophoresis. Results showed that primers for pathogenic strains did not amplify DNA from B,C, G, T strain, and no specific band was observed on gel eleotrophoresis....

The polymerase chain reaction (PCR) is an in vitro DNA amplification procedure. With the participation of polymerase, dNTP and their primers, specific DNA of known sequences increased greatly in a few hours. Four strains (B, C, G, T) of E. histolytica DNA were used and PCR repeated for 30 cycles. The PCR products were put to gel eleotrophoresis. Results showed that primers for pathogenic strains did not amplify DNA from B,C, G, T strain, and no specific band was observed on gel eleotrophoresis. But primers specific for nonpathogenio strains amplified DNA from B, C, G, T strain, and a specific band was observed on gel analyses. Horseradish peroxidase-labelled pathogenic strain DNA probes did not react with PCR products of B, C, G, T strains; on contrary, nonpathogenio probes reacted, demonstrating that B, C, G, T belonged to nonpathogenio strains. These results corresponded to isoenzyme analyses. PCR is a highly sensitive and specific method for identification of pathogenic and nonpathogenio isolates of E. histolytica. It provides a new method for studying the pathogenioity of E. histolytica on a molecular basis.

多聚酶链反应是体外DNA增殖的新技术。在多聚酶、dNTP和引物的参与下在数小时内使特异性的DNA顺序大量增殖。用B、C、G、T4株溶组织内阿米巴的DNA增殖30周期后,作凝胶电泳分析,发现致病性虫株的引物不能使B、C、G、T虫株的DNA增殖,凝胶电泳不出现条带;而非致病性虫株的引物能使B、C、G、T虫株的DNA增殖,凝胶电泳出现特异性条带。用辣根过氧化物酶标记致病性虫株DNA探针与B、C、G、T的DNA进行斑点杂交呈阴性反应;而用非致病性虫株DNA探针与B、C、G、T的DNA斑点杂交呈阳性反应。实验结果显示B、C、G、T为非致病性虫株,与同工酶分析一致。多聚酶链反应是鉴别致病性和非致病性溶组织内阿米巴虫株的高特异性和敏感性方法,为从分子生物学研究溶组织内阿米巴的致病机理提供一新技术。

 
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