BACKGROUND & OBJECTIVE: Bcl-2/ElB 19kDa interacting protein3-like (BNIP3L) gene is a tumor suppressor gene cloned from a human fetal liver cDNA library, locating at 8p21, one of the high frequent LOH regions in lung carcinoma.
背景与目的：BCl-2／ElB 19 kDa-interacting protein 3-like (BNIP3L)基因是从人胎肝cDNA文库中克隆得到的具有抑瘤活性的新基因，定位于8p21肺癌高频杂合性丢失(loss of heterozygosity，LOH)区；
BACKGROUND &OBJECTIVE: Bcl-2/E1B 19kDa interacting protein3-like (BNIP3L) gene is a tumor suppressor gene cloned from a human fetal liver cDNA l ibrary, which is located at 8p21, one of the high frequent regions of loss of he terozygosity (LOH) in lung carcinoma.
It is, therefore, reasonable to conclude that translated HGF is the same substance as biochemically purified one and that HGF of human fetal liver origin is the gene expression product of fetal liver cells.
Methods The target cDNA fragment was amplified from the total RNA extracted from human fetal liver tissue by RT-nested PCR, cloned into pGEM-T vector and identified by restriction analysis and sequencing.
A novel transcript of human HPO (HPO-205) encoding a 205 amino acid ORF corresponding to a translated product of 23 kD different from the previous HPO that lacks the N-terminal 80 amino acids, was isolated from human fetal liver cDNA library by 5′ RACE methods.
A novel gene, named embryonic develop associated gene 1 ( EDAG 1) and abundantly expressed in human fetal liver tissues, was isolated by screening a human fetal liver cDNA library and the 5′RACE. The full length of EDAG 1 mRNA is 2 166 bp, with an open reading frame of 1 452 bp neucleotides, encoding a 484 amino acid protein.
We have studied AAT expression infusion hybrids between a rat hepatoma line and either human fetal liver fibroblasts (series XXII) or human skin fibroblasts (series XIX).
Activation of humanα1-antitrypsin gene in rat hepatoma × human fetal liver cell hybrids depends on presence of human chromosome
At present the most promising results for BAL application have been obtained by immortalization of human fetal liver cells by reconstitution of telomerase activity.
To investigate the role of HCCA2 in HCC genesis and progression, we screened a human fetal liver cDNA library and identified a novel HCCA2-interacting protein, MAD2L2 (MAD2 mitotic arrest deficient-like 2 (yeast)).
In a two-hybrid screen of human fetal liver cDNA library, TH1 was detected as a new interaction partner of A-Raf.