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   restriction enzyme analysis 的翻译结果: 查询用时:0.214秒
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restriction enzyme analysis     
相关语句
  酶切
     The results were as follows:1.Recombinant plasmids PcDNA3.1-S2S-GM-CSF, PcDNA3.1-S2Sc, PcDNA3.1-S-GM-CSF and PcDNA3.1-Sc were successfully constructed respectively, and identified by restriction enzyme analysis, PCR amplification and/or DNA sequencing.
     1. 重组质粒PcDNA3.1-S2S-GM-CSF, PcDNA3.1-S2Sc,PcDNA3.1-S -GM-CSF和PcDNA3.1-Sc经酶切、PCR和DNA测序鉴定构建正确。
短句来源
     The bait recombinant vectors Met-G1and Met-G1' were proved to be successfully constructed by restriction enzyme analysis.
     限制性内切酶酶切鉴定表明,Met-G1和Met-G1'载体构建正确。
短句来源
     Methods: Genomic DNA from 8 unrelated PK deficiency patients was studied by polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) and restriction enzyme analysis.
     方法 :应用聚合酶链反应 -单链构象多态性 (polym erase chain reaction- single strand conformation polymorphism ,PCR- SSCP)和限制性内切酶酶切方法 ,分析上海地区 8个 PK缺陷症家系红细胞 PK基因变异的情况。
短句来源
     C279G mutant UCHL1 was obtained through gene splicing by overlap extension (SOE) on site direct mutagenesis of wild-type human UCHL1. The wild type and C279G mutant UCHL1 gene were cloned into pEGFP-N1, and identified by restriction enzyme analysis and sequencing.
     再采用SOE法定点突变,通过酶切和连接,构建野生型和C279G突变型pEGFP-N1UCHL1。
短句来源
     Efficient expression of the Cry1Ac gene in the engineered JAAS01D_1Ac was proved with restriction enzyme analysis, SDS_PAGE electrophoresis analysis and insecticidal activity assay.
     工程菌株质粒酶切电泳分析、SDS_PAGE电泳分析和杀虫生物活性测定结果证实了Cry1Ac基因的导入及其在枯草芽孢杆菌JAAS01D中的有效表达。
短句来源
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  酶切分析
     The recombinant plasmid pEGFPTTV2 was identified by restriction enzyme analysis and PCR.
     构建出的重组质粒pEGFPTTV2经过酶切分析和PCR鉴定。
短句来源
     To determine gene mutations of achondroplasia in Chinese and establish a simple, rapid, sensitive and specific molecular diagnostic method, the mutations of the fibroblast growth factor receptor 3 (FGFR3) gene of 12 patients with achondroplasia, the parents of 2 sporadic cases, and 4 normal controls wereassayed by polymerase chain reactionrestriction enzyme analysis.
     为了解软骨发育不全基因突变类型,采用聚合酶链反应-限制性酶切分析的方法,对12例软骨发育不全患者、2例散发患者的父母和4例健康正常人的成纤维细胞生长因子受体3(FGFR3)基因位点的点突变进行检测。
短句来源
     These two fragments were cloned into the pMD-18T vector for the partial sequencing and restriction enzyme analysis.
     将扩增产物克隆到pMD-18T上,经酶切分析、部分序列测定和同源比较确认得到了所需的基因片段。
短句来源
     RESTRICTION ENZYME ANALYSIS OF MAREK’S DISEASE VIRUS GLYCOPROTEIN B ANTIGEN GENE CLONE
     马立克病病毒糖蛋白B抗原基因克隆的酶切分析
短句来源
     Restriction Enzyme Analysis and Molecular Cloning of CAV-1 DNA
     犬Ⅰ型腺病毒DNA的酶切分析及分子克隆
短句来源
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  限制性内切酶分析
     Results: The 1136 bp long cDNA encoding human CK2α subunit was obtained from human Hepatoma-3B cells ( Hep-3B) by RT-PCR. The PCR product was directly cloned into pUCm-T vector by ligation of cohesive end. And the recombined plasmid of pUCm-T-CK2α was identified by PCR and restriction enzyme analysis.
     结果通过RT-PCR从Hep-3B肝癌细胞中获得人蛋白激酶CK2α(ProteinKinase2α)亚基编码区1136bp的cDNA片段,将扩增基因通过粘端连接克隆到pUCm-T载体中,用PCR和限制性内切酶分析鉴定,表明获得重组质粒pUCm-T-CK2α。
短句来源
     Identification of 12 Mycobacteria to the Species Level by Polymerase Chain Reaction and Restriction Enzyme Analysis
     采用PCR法和限制性内切酶分析对12种分枝杆菌进行鉴定
短句来源
     acervulina, which was isolated from an outbreak in Guangdong Province, as a template, a partial segment of Ea1A was amplified by RT-PCR. The Ea1A gene fragment was cloned into pGEM-T Easy vector, and the recombinant plasmid was named pGEM-Ea1A. Finally, the plasmid was identified by PCR, restriction enzyme analysis and sequencing.
     根据GenBank上登录的堆形艾美球虫Ea1A基因序列 ,设计了 1对引物 ,以抽提的广东株的总RNA为模板 ,利用反转录 聚合酶链反应 (RT PCR)扩增获得了Ea1A基因部分片段 ,并将此片段克隆至pGEM T载体中 ,经PCR、限制性内切酶分析和克隆片段序列测定、比较 ,证实了克隆片段的可靠性
短句来源
     Methods:Twenty-eight normal individuals and 18 patients with throm botic diseases were studied by APC-APTT,PCR followed by Mnl Ⅰ restriction enzyme analysis,PCR SSP,and DNA sequence analysis.
     方法:用活化的蛋白C(APC)-APTT,多聚酶链反应(PCR)及限制性内切酶分析,PCR-SSP及DNA序列分析,对28例正常人及18例血栓性疾患患者进行APC测定及FⅤLeiden基因突变分析。
短句来源
     The recombinant plasmid pBV220/tk was identified with restriction enzyme analysis, and the recombinant protein with SDS PAEG electrophoresis and Western blot assay.
     采用限制性内切酶分析进行重组质粒的鉴定、 SDS- PAEG电泳和 Western blot反应检测蛋白的表达。
短句来源
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  限制性酶切分析
     To determine gene mutations of achondroplasia in Chinese and establish a simple, rapid, sensitive and specific molecular diagnostic method, the mutations of the fibroblast growth factor receptor 3 (FGFR3) gene of 12 patients with achondroplasia, the parents of 2 sporadic cases, and 4 normal controls wereassayed by polymerase chain reactionrestriction enzyme analysis.
     为了解软骨发育不全基因突变类型,采用聚合酶链反应-限制性酶切分析的方法,对12例软骨发育不全患者、2例散发患者的父母和4例健康正常人的成纤维细胞生长因子受体3(FGFR3)基因位点的点突变进行检测。
短句来源
     Conclusions Nested PCR combined with restriction enzyme analysis would be effective in rapid detection of HCMV.
     结论 Nested PCR加限制性酶切分析是一种临床检测HCMV的快速有效手段
短句来源
     The primers specific for the protective antigen(PA) of Bacillus anthracis coding sequence was designed and synthesized. The PA gene was cloned from pOX1 plasmid by using PCR and inserted into vector pET-28a to obtain the recombinant expressing plasmind pET-28a-PA. The restriction enzyme analysis and DNA sequence detection confirmed that the inserted fragment of clone pET-28a-PA is the mature PA coding sequence.
     按照炭疽芽孢杆菌保护性抗原(PA)基因成熟肽编码序列设计引物,从炭疽杆菌pOX1质粒中扩增出PA基因片段,将该片段定向插入到原核表达载体pET-28a中,获得了pET-PA原核表达重组质粒,限制性酶切分析和DNA序列测定均证实该克隆插入片段为PA基因的成熟肽编码序列。
短句来源
     and the results were further confirmed by restriction enzyme analysis of the genomic DNA fragments.
     限制性酶切分析其基因组DNA。
短句来源
     Rapid Preparation and Restriction Enzyme Analysis of the DNA of Canine Adenovirus Type 2
     犬Ⅱ型腺病毒DNA的快速提取及限制性酶切分析
短句来源
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  restriction enzyme analysis
Comparative Restriction Enzyme Analysis of the Genome in Variola Virus Strains from the Russian Collection
      
The conventional incompatibility test and restriction enzyme analysis revealed three new IncP-9 subgroups: ζ, η, and IncP-9-like.
      
In addition to the known nucleotide sequences of nahG and nahAc, two novel nahG variants were revealed by a restriction enzyme analysis of amplification products.
      
An amplified rDNA restriction enzyme analysis (ARDRA) demonstrated that the native hosts of IncP-9 Nah plasmids were fluorescent bacteria of the genus Pseudomonas (P.
      
The PON1 alleles were identified using PCR and restriction enzyme analysis.
      
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A rapid extraction procedure for plasmid DNA and the effect of experimental conditions on the results were studied. The results showed that the procedure might be simplified. Plasmid DNA extracted in this way is pure enough to be used in transformation experiment and restriction enzyme analysis. The method is simple enough to be used in screening recombinant plasmid DNA and doing molecular genetics experiment.

本文研究在各种实验条件下快速抽提质粒DNA.结果获得更为简化的方法.用这种方法抽提到的质粒DNA其纯度足以用来转化细菌以及内切酶分析,由于非常简单所以特别适用于重组质粒DNA的筛选以及分子遗传学的实验.

The genetic homology of seven NPV genomes have been studied by restriction enzyme pattern analysis and DNA hybridization utilizing 32P-label-led NPV-DNA as probes. The resits showed that the restriction enzyme patterns of NPV-DNA among five NPV infecting different host species were different, but those between isolates from the same host species were almost the same except for some fragments with different migration rate and some submolar fragments. Detection of genetic homology by DNA hybridi-zation revealed...

The genetic homology of seven NPV genomes have been studied by restriction enzyme pattern analysis and DNA hybridization utilizing 32P-label-led NPV-DNA as probes. The resits showed that the restriction enzyme patterns of NPV-DNA among five NPV infecting different host species were different, but those between isolates from the same host species were almost the same except for some fragments with different migration rate and some submolar fragments. Detection of genetic homology by DNA hybridi-zation revealed that the major DNA fragments of NPV from the same host species hybridized with homologous probes. No hybridization can be observed between NPV-DNA from different host species. This report suggests that restriction enzyme analysis and molecular hybridization are of value for characterization of this group of viruses.

应用限制性内切酶图谱分析法,结合Southern印迹法和核酸杂交技术,对茶毛虫、棉蛉虫,油桐尺蠖、斜纹夜蛾以及蓖麻蚕等5种昆虫的7株核型多角体病毒DNA,进行了基因组同源性测定。结果表明,不同种昆虫多角体病毒DNA的酶切图谱不相同,DNA片段与不同源的DNA标记探针之间无杂交带出现。而同种昆虫病毒的不同分离株间,除少数DNA片段的电泳迁移率稍有不同,以及出现一些互不相同的亚克分子带之外,它们的DNA酶切图谱基本一致,並且几乎所有片段都可与同种的标记探钟杂交。对一些DNA片段迁移率的改变及亚克分子带出现的原因进行了讨论。

Further studies on HincⅡ polymorphic restriction site 5' to e globin gene of human DNAs from normai Zhuan individuals members of Han families and β-thalassemia patients have been carried out by restriction enzyme analysis. The frequency of the HincⅡ site polymorphism among Zhuan people and β-thalassemia patients were found to be 75%,Which was rather close to that previously reported among Han people. Results from family study showed that the inheritance of the polymorphic site among family members seemed...

Further studies on HincⅡ polymorphic restriction site 5' to e globin gene of human DNAs from normai Zhuan individuals members of Han families and β-thalassemia patients have been carried out by restriction enzyme analysis. The frequency of the HincⅡ site polymorphism among Zhuan people and β-thalassemia patients were found to be 75%,Which was rather close to that previously reported among Han people. Results from family study showed that the inheritance of the polymorphic site among family members seemed to follow Mendel's rule.

本文进一步研究了我国不同民族的正常个体以及β地中海贫血患者θ珠蛋白基因5′侧序列中的多态性HincⅡ位点及其遗传性质。在广西壮族正常个体和β地中海贫血纯合子中,该多态性位点的发生频率均为75%,与正常汉族人测得值相近。家系分析资料表明,该多态性位点完全按照孟德尔规律进行遗传。

 
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