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rapd
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  rapd技术
    STUDY ON DNA POLYMORPHISMS OF FOUR SPIROPLASMA STRAINS BY RAPD TECHNOLOGY
    用RAPD技术对4种螺原体菌DNA多态性的初步研究
短句来源
    In this paper,the genetic relationships among six fruit fly species of Bactrocera in Yunnan Province of Southwest China,i. e. ,B.tau,B.scutellaris,B.scutellata,B.cucurbitae,B.dorsalis and B.correcta,were studied by using the technique of random amplified polymorphic DNA(RAPD).
    采用RAPD技术构建了果实蝇属(Bactrocera)的南瓜实蝇(B.tau)、黑漆实蝇(B.scutellaris)、具条实蝇(B.scutellata)、瓜实蝇(B.cucurbitae)、桔小实蝇(B.dorsalis)和番石榴实蝇(B.correcta)6种实蝇的指纹图谱.
短句来源
    Study on the Genetic Variation of Physalospore piricola in Apple by Using RAPD Technique
    用RAPD技术研究我国苹果果实轮纹病菌的遗传变异(英文)
短句来源
    Identification of brown spot disease resistance gene from flue-cured tobacco by RAPD technique
    用RAPD技术鉴定烤烟抗赤星病基因连锁标记
短句来源
    The technique of RAPD and its application in the studies of plant pathology
    RAPD技术及其在植物病理学上的应用
短句来源
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  rapd分析
    USING RAPD ANALYSIS AND GENETIC FINGERPRINTING TO DIFFERENTIATE ISOLATES OF RACE O,C AND T OF COCHLIOBOLUS HETEROSTROPHUS
    用RAPD分析和DNA指纹鉴别玉米小斑病菌O、C、T三个小种
短句来源
    RAPD ANALYSIS OF TWO GEOGRAPHIC POPULATIONS OF COTTON BOLL WORM
    两个不同地区棉铃虫种群的RAPD分析
短句来源
    RAPD Analysis of Verticillium dahliae from Cotton in Northern China.
    北方棉区棉花黄萎病菌RAPD分析
短句来源
    RAPD Analysis of Physiological Races of Rice Blast Fungus (Pyricularia grisea) and Its Difference to Digitaria Blast
    稻瘟病菌生理小种RAPD分析及其与马唐瘟的差异
短句来源
    Phylogentic Relationships Between Alternaria longipes and A. alternata Revealed by RAPD
    长柄链格孢(Alternaria longipes)和链格孢(A.alternata)的RAPD分析
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  rapd标记
    Evaluation of Disease Resistances in Chinese Wild Vitis and Genetic Mapping for Disease Resistance Genes in Vitis Using RAPD Markers
    中国葡萄属野生种抗病性及抗病基因RAPD标记作图研究
短句来源
    RAPD markers OPAI17-1550 and OPAI13-900,linked to anti-black-spot disease gene locus,were successfully converted into SCAR markers named SAI17-1570 and SAI13-292 respectively by sequencing and designing of primers. SAI17-1570 and SAI13292 were validated in the resistant、susceptible pools and F1 population.
    通过测序和引物设计,将与美洲黑杨抗黑斑病基因相连锁的RAPD标记OPAI17-1550和OPAI13-900成功地转化成显性SCAR标记(SAI17-1570)和共显性SCAR标记(SAI13-292),并对感、抗病池和F1代91个无性系进行了SCAR标记检测。
短句来源
    RAPD Markers Associated with Resistance to Soybean Cyst Nematode
    与大豆孢囊线虫病抗性相关的RAPD标记
短句来源
    THE WHEAT BREEDING STUDY IN HIGH PRODUCTIVE STRAIN RESISTANT TO POWDERY MILDEW THE RAPD ANALYSIS IN F 2
    小麦F_2代白粉病抗、感病特性RAPD标记的比较研究
短句来源
    RAPD MARKER OF RESISTANCE GENE TO STRAIN GPV OF BYDV IN WHEAT ADDITION LINE Z1
    抗大麦黄矮病毒GPV株系小麦异附加系Z1抗性基因的RAPD标记
短句来源
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  “rapd”译为未确定词的双语例句
    Genetic Diversity of Soybeans Germplasm on Cercospora sojina by RAPD and SSR
    大豆灰斑病种质资源遗传多样性的RAPD和SSR分析
短句来源
    Study on Tomato RAPD Clustering, cDNA Library Construction and Separating RGA from ToMV-resistance Tomato(Lycopersicum Esculentum Mill)
    番茄RAPD聚类分析、抗ToMV番茄cDNA文库构建和分离RGA的研究
短句来源
    Identification of RAPD Fragments Related to Rice Resistance Gene to Blast and eui Gene
    RAPD方法鉴定水稻抗瘟性和eui基因相关的DNA片段
短句来源
    (2) fine mapping of the resistance gene Gm6 in rice germplasm `Daqiuqi' from China using RAPD and SSR markers;
    (2)分别用RAPD和微卫星SSR技术对来源于我国的水稻资源大秋其的抗亚洲稻瘿蚊基因Gm6进行精细定位;
短句来源
    Genetic variation of 26 strains of Cryphonectria parasitica from Korea,USA and China was investigated using random amplified polymorphic DNA(RAPD) of genomic DNA.
    用RAPD方法,对来自韩国、美国和中国的26个栗疫病菌菌株进行了遗传变异分析。
短句来源
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  rapd
Genetic Diversity of RAPD Mark for Natural Davidia involucrata Populations
      
The genetic diversity and genetic variation within and among populations of five natural Davidia involucrata populations were studied from 13 primers based on random amplified polymorphic DNA (RAPD) analysis.
      
This paper studies the genetic diversity and differentiation of its nine natural populations in Zhejiang Province by using random amplified polymorphic DNA (RAPD) technique.
      
RAPD analysis of genetic relationships among Sphaeropsis sapinea isolates
      
Genetic relationships were studied among 23 isolates of Sphaeropsis sapinea collected from China, the United States, England, South Africa and Chile by using a random amplification of a polymorphic DNA (RAPD) analytical method.
      
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A RAPD marker,OPV-10390 , linked to the Oidium heveae-esistancegene of Hevea brasiliensis was cloned and its DNA sequence was determined. Nucleotide sequence analysis by DNASISTM system indicates that the fragm-ent was not all encoding seqtience and had no conservative sequence of 5‘-flanking region and 3‘- flanking region,but a structure similar to exon andintron.

对橡胶树抗白粉病基因连锁RAPD标记OPV—10390进行了克隆和测序。经计算机DNAsis系统分析,结果表明,该片段不全是编码序列,也没发现结构基因5′侧翼区和3′侧翼区的保守序列,但有近于外显子和内含子的结构。

Chinese reference isolates of predominant races of Puccinia striiformis f.sp. tritici, the causal agent of wheat stripe rust disease, were examined for genetic variation with the random amplified polymorphic DNA (RAPD) assay. Random decamer primers were used to amplify DNA isolated from 11 reference isolates of the 11 predominant races ,CY17,CY19,CY21,CY22,CY23, CY25,CY26,CY27,CY28,CY29,and Shuiyuan 11-1,respectively,and 5 isolates sampled form geographically separated regions belonging to two newly identified...

Chinese reference isolates of predominant races of Puccinia striiformis f.sp. tritici, the causal agent of wheat stripe rust disease, were examined for genetic variation with the random amplified polymorphic DNA (RAPD) assay. Random decamer primers were used to amplify DNA isolated from 11 reference isolates of the 11 predominant races ,CY17,CY19,CY21,CY22,CY23, CY25,CY26,CY27,CY28,CY29,and Shuiyuan 11-1,respectively,and 5 isolates sampled form geographically separated regions belonging to two newly identified pathotypes (races),CY30 and CY31.of 114 RAPD loci amplified with 17 primers,75.4% were polymorphic. Genetic relatedness among the 16 reference isolates was determined by hierarchical cluster analysis of 58 selected RAPD loci generated by 17 random decamer primers. A lack of correlation between the virulence and RAPD dissimilarity matrices was observed, indicating the independence of DNA polymorphism from virulence diversity. DNA polymorphism was detected among isolates of different as well as of single races. RAPD patterns generated by some primers enabled us to resolve distinctly all the isolates tested. Compared with other attempts to develop sufficient markers for genetic analysis of P. striiformis f. sp. tritici, RAPD procedure is technically simple, rapid, and reproducible, and has potential application for stringent genetic characterization of the population structure of this obligata pathogen.

用随机扩增多态性DNA(RandomamplifiedpolymorphicDNA,RAPDR)技术对中国小麦条锈菌(Pucciniastriiformisf.sp.tritici)流行小种的11个模式分离系(CY17,CY19,CY21,CY22,CY23,CY25,CY26,CY27,CY28,CY29,水源11-1)以及5个分属于两个新发现小种CY30和CY31的分离系进行了基因组DNA多态性分析.用17个10-核苷酸随机引物共获得114个RAPD标记,其中75.4%表现多态性.选取其中的58个RAPD标记通过系统聚类分析确定了供试分离系间的亲缘关系,并与以毒性标记为基础确定的分离系间的演化关系进行了比较.结果表明,DNA多态性与毒性多态性之间没有相关性.利用RAPD标记检测到了小种间以及小种内的遗传变异,有些引物扩增到了分离系特异的RAPD特征图谱。与其它基因组多态性分析技术相比,RAPD分析可为小麦条锈菌的遗传分析提供大量的分子标记,且具有技术操作简单、快速、安全以及仅需微量的模板DNA等优点,对该活体营养病菌的群体遗传结构分析极具潜力.

K80R and K79S were a pair of near iso-genie lines with blast resistance genes. They were derived from progenies of three backcrossing followed by nine self-crossing of Hong-Jiao-Zhan as the donor of blast resistance genes and IR24 as the recurrent parent. One hundred and seventy-seven RFLP probes were screened among these four varieties and 10 positive probes were isolated. Plants of F2 population of K80RX K79S were inoculated with Pyricularia aryzae Cav. race ZB, and were scored 110 resistant: 33 susceptible....

K80R and K79S were a pair of near iso-genie lines with blast resistance genes. They were derived from progenies of three backcrossing followed by nine self-crossing of Hong-Jiao-Zhan as the donor of blast resistance genes and IR24 as the recurrent parent. One hundred and seventy-seven RFLP probes were screened among these four varieties and 10 positive probes were isolated. Plants of F2 population of K80RX K79S were inoculated with Pyricularia aryzae Cav. race ZB, and were scored 110 resistant: 33 susceptible. RFLPs of each F2 plants were tested by the positive probes. RG81, RG869 and RZ397 on chromosome 12 were found to be linked with a blast resistance gene, which was named Pi-h-1 (t). Genotypes of each F2 plants were monitored by investigating the reaction to P. aryzae of each F3 lines and genetic distances between Pi-h-1 (t) and RFLP markers were calculated. Two PCR amplified fragments were found to be tightly linked to Pi-h-1 (t) by RAPD analysis.of near isogenic pools of DNA from F2 plants.

从水稻分子遗传图谱选取177个RFLP标记,比较以红脚占为抗源,感病品种IR24为轮回亲本所构建的近等基因对K80R和K79S之间的多态性表现。发现了一些可能与稻瘟病抗性基因连锁的阳性标记。在(K80R×K79S)的F_2群体中,经稻瘟病菌ZB_1小种接种,110株为抗病,33株为感病,用总共10个阳性标记与F_2群体中每个单株的DNA杂交,发现抗病基因与第12染色体上的标记RG81、RG869和RZ397共分离;检测F_3株系的抗病性分离情况,确定F_2植株的抗病性基因型,计算出抗病基因与分子标记的遗传距离,将该基因定位在第12连锁群上。应用近等基因池DNA和随机引物,经PCR扩增和共分离分析,建立了二个RAPD片段与抗病基因紧密连锁。

 
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