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transplantation tumors     
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  移植瘤
     Methods The content of ERK1/2, JNK, p38, c-fos and c-jun of transplantation tumors of large bowel carcinoma of nude mice was detected by laser scanning confocal microscopy. ERK1/2, JNK and p38 was belonging to mitogen-activated protein kinase family.
     方法 以激光共聚焦显微镜检测大肠癌裸鼠移植瘤丝裂原活化的蛋白激酶家系中的ERK1 2、JNK和p38以及激活蛋白 1的主要组分c fos和c jun的含量。
短句来源
     And the nude mouse transplantation tumors inoculated cells transfected MCTl antisense gene were significantly lighter and smaller than that ones inoculated A549 cells ( P < 0.001) .
     接种转染MCT1反义基因的细胞移植瘤明显比接种A549细胞的轻且小(P<0.001)。
短句来源
     RESULTS: The positive cell density of NF-κB of large bowel carcinoma transplantation tumors in bifidobacterium injection group was markedly lower than that in tumor control group( P< 0.01).
     结果 :双歧杆菌注射组大肠癌移植瘤NF -κB的阳性细胞密度明显低于肿瘤对照组 (P <0 0 1) ;
短句来源
     Results:After nude mouse transplantation tumors of large bowel carcinoma were treated with WPG; its average fluorescent strength of IκB α was significantly higher than that in tumor control group( P <0 01);
     结果 :大肠癌裸鼠移植瘤经WPG处理后 ,其IκBα的平均荧光强度明显高于肿瘤对照组 (P <0 0 1) ;
短句来源
     And the transplantation tumors of nude mice inoculated co-transfected antisense gene-cells and inoculate d MCT1-antisense-gene-transfecte d cells were significantly lighter a nd smaller than ones inoculated A549cells(P<0.001).
     接种联合转染反义基因细胞和转染MCT1反义基因细胞的裸鼠移植瘤明显比接种A549细胞的轻且小(P<0.001)。
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  移植瘤的
     The inhibitory rates of lingzhi-912 on S180, HCA and Lewis transplantation tumors were 45.1%~56.7%,33.3%~52.7% and 48.1%~56.6% respectively (P <0.01).
     lz - 912对S180 ,HCA和Lewis小鼠移植瘤的抑制率分别达 4 5 .1%~ 5 6 .7% ,33.3%~5 2 .7%和 4 8.1%~ 5 6 .6 % (P <0 .0 1) ;
短句来源
     Objective: To observe the influence of connexin 43 (Cx43) on the bystander effect induced by cytosine deaminase (CD) and herpes simplex virus thymidine kinase (HSV-tk) coexpression suicide genes system in human cholangiocarcinoma QBC939 cells and transplantation tumors in nude mice.
     目的观察胞嘧啶脱氨酶(cytosine deaminase,CD)基因和单纯疱疹病毒胸苷激酶(herpes simplex virus thymidine kinase,HSV-tk)基因对胆管癌细胞和胆管癌移植瘤的抑制作用以及转染缝隙连接蛋白43(connexin43,Cx43)基因对旁观者效应的影响。
短句来源
     The cells co-transfected antisense genes,cells transfected MCT1antisense gene and A549cells were respectively inoculated in nude mouse subcutaneous to observe the growth o f transplantation tumors.
     并将联合转染反义基因的细胞、转染MCT1反义基因的细胞、A549细胞分别接种于裸鼠皮下,观察移植瘤的生长。
短句来源
     In order to investigate the influence of Cx43 on the bystander effect, the size of transplantation tumors of the CD+tk+ and CD+tk+Cx+ cells was measured before and after application of 5-Fc and GCV. Results: CD and tk genes were stably expressed in transfected QBC939 cells.
     前体药物应用前后测量移植瘤的大小判断自杀基因系统的作用和Cx43蛋白对旁观者效应的影响。 结果CD和tk基因在转染细胞中获稳定表达。
短句来源
     Methods The growth rate, ultrastructure, density of apoptotic cells and positive expression rate of the Bcl 2 and Bax oncoprotein were investigated in nude mouse transplantation tumors of colorectal carcinoma by electronmicroscopy, in situ end labeling and immunohistochemistry, depending on the animal model of nude mouse transplantation tumors of colorectal carcinoma.
     方法 以大肠癌裸鼠移植瘤为动物模型,观察移植瘤的生长速度,同时利用透射电镜、原位末端标记技术及免疫组化技术原位观察了大肠癌裸鼠移植瘤的超微结构、凋亡细胞密度以及Bcl2 、Bax 蛋白表达率及其阳性细胞密度。
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  移植性肿瘤
     Suppressive effect of a novel recombinant human tumor necrosis factor-α on the growth of different transplantation tumors in mice
     新型基因工程人肿瘤坏死因子-α对小鼠移植性肿瘤生长的抑制作用
短句来源
     AimTo observe the suppressive effect of a novel recombinant human tumor necrosis factor α(nrhTNF α) on the growth of different transplantation tumors in mice.
     目的 观察新型基因工程人肿瘤坏死因子α(nrhT NF α) ,对小鼠移植性肿瘤 (肉瘤S180、肝癌H2 2、黑素瘤B16和Lewis肺癌 )生长的抑制作用。
短句来源
     ConclusionnrhTNF α possesses a very strong suppression on growth of transplantation tumors.
     结论nrhTNF α对小鼠移植性肿瘤生长具有很强的抑制作用。
短句来源
     Methods: The antitumor effect of different concentration of lingzhi-912 were observed on animal model of transplantation tumors in mice: (ECA), Sarcoma(S180), Hepatic carcinoma(HCA), Lewis lung cancer. The positive control group was treated with 5-Fu. Hemolysin experiment and tardive allergic reaction experiment were used to observed the effect of lingzhi-912 on immune function on transplantation tumor mice.
     方法 :采用小鼠移植性肿瘤艾氏腹水癌 (ECA) ,肉瘤180 (S180 ) ,肝癌 (HCA) ,Lewis肺癌模型 ,5 -Fu为阳性对照组 ,观察不同浓度 1z - 912的抗肿瘤作用 ,并采用小鼠溶血素实验和小鼠迟发性变态反应实验观察Iz~ 912对荷瘤小鼠免疫功能的影响。
短句来源
  移植瘤组织
     Methods:The animal model of large bowel carcionma nude mouse transplantation tumors was set up. Bifidobacteria adolescence were intraperitoneally injected into nude mice in advance. The content of ERK1/2、JNK and p38 of mitogen activated protein kinase(MAPK) family in large bowel carcinoma transplantation tumor tissues was detected by using laser scanning confocal microscopy.
     方法 :以大肠癌裸鼠移植瘤为动物模型 ,预先用青春型双歧杆菌注射于裸鼠腹腔 ,然后以激光共聚焦显微镜检测大肠癌移植瘤组织丝裂素活化的蛋白激酶 (MAPK)家系中的ERK1/ 2、JNK和p38的含量。
短句来源
     Purpose To observe the relationship between the expression of formylpeptide receptor (FPR) and production of vascular endothelial growth factor (VEGF) in human glioma cell line U87 orthotopic transplantation tumors in the brains of nude mice, and to explore the potential roles of FPR during the angiogenesis of malignant glioma.
     目的观测甲酰肽受体(formylpeptide receptor,FPR)在人恶性胶质瘤细胞系U87细胞裸鼠脑原位移植瘤组织中的表达,以及与血管内皮生长因子(VEGF)的关系,探讨FPR在恶性胶质瘤血管生成过程中的作用。
短句来源
     Many apoptotic cells of transplantation tumors in the whole peptidoglycan group were observed. The density of apoptotic cells, expression rate and positive cell density of Bax oncoprotein were significantly higher than those in the whole peptidoglycan injection group when compared with those in the tumor control group ( P < 0.01 ). The expression rate of Bcl 2 oncoprotein was contrary to that of Bax oncoprotein.
     此外完整肽聚糖注射组移植瘤组织中可见多量的处于凋亡不同时期的瘤细胞,其凋亡细胞密度、Bax 蛋白表达率以及阳性细胞密度均显著高于肿瘤对照组( P<0 .01),而Bcl2 蛋白的表达情况则相反。
短句来源
     The percentage of apoptosis cells in the transplantation tumors in the bifidobacterium injection group and the tumor control group was counted by employing in situ end labeling technique, depending on the animal model of nude mice transplantation tumors of colorectal cancer.
     本文以大肠癌裸鼠移植瘤为动物模型,将实验分为双歧杆菌注射组以及肿瘤对照组,用原位末端标记法观察了移植瘤组织的凋亡细胞百分率。
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  transplantation tumors
The inoculation of transplantation tumors at different sites resulted in different chemotherapeutic responses to cyclophosphamide.
      
In five cases, 7-33 months after autotransplantation we found transplantation tumors developing in the forearm.
      
In the series of transplantation tumors S-100 and GFAP immunoreactivity decreased with increasing numbers of transplantation passages.
      
In contrast, GFAP was expressed in only 23/51 primary tumors and in 5/90 transplantation tumors.
      
Vimentin immunoreactivity was shown in 50/51 primary neurinomas and 60/90 transplantation tumors.
      
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The Fugu medicated therapeutic prescription (FGMTP) composed of the medicinal herbs with food usage was investigated on the nutrition components and its antitunor activity. The results showed that the ratios of thermogenesis of three calorifacient nutrients were protein (12.34%), fat(5.62%) and carbohydrates (82.0%), respectively. The IC50 values of the water extract from this prescription against the 7901 or K562 cell line colony formations were 3.59g/L and 25.74 mg/L, and the maximal inhibitory rates...

The Fugu medicated therapeutic prescription (FGMTP) composed of the medicinal herbs with food usage was investigated on the nutrition components and its antitunor activity. The results showed that the ratios of thermogenesis of three calorifacient nutrients were protein (12.34%), fat(5.62%) and carbohydrates (82.0%), respectively. The IC50 values of the water extract from this prescription against the 7901 or K562 cell line colony formations were 3.59g/L and 25.74 mg/L, and the maximal inhibitory rates against both cell line colony formations were 53.2% and 58.9%; respectively. The maximal inhibitory rates against HepA and S180 transplantation tumors were 35.3% and 35.0%, respectively. It is concluded that the FGMTP possessed obvious antitumor effects.

为观察硒对肺炎支原体肺炎的治疗效果,采用双盲随机对照实验:选择住院支原体肺炎患儿,分为补硒组26例,病例对照组21例,正常组21例。一次补硒量为1mg亚硒酸钠,对照病例组给予常规治疗。临床结果表明,与对照组相比,治疗后补硒组的症状和体征缓解天数均有不同程度缩短(P<0.05),补硒组白细胞硒和谷胱甘肽过氧化物酶水平显著上升(P<0.05),细胞免疫功能有所改善。本研究结果提示,感染时机体内硒的代谢加速,使硒的需要量增加,补硒可减轻病情和/或加速病变恢复

Objective To evaluate the therapeutic effect of the new method-adoptive cell immunotherapy on ovarian carcinoma. Methods Tumor soluble antigen (TSA) was extracted from cells of ovarian carcinoma (COC 1 and COC 2n ). To generate cytotoxic T lymphocytes (CTL), peripheral blood mononuclear cells (PBMC) collected from healthy persons were induced by TSA and anti CD 3 monoclonal antibody (CD 3 McAb). The antitumor effect of CTL on COC 1 cells in vitro was compared with LAK and CD 3 AK, whereas the...

Objective To evaluate the therapeutic effect of the new method-adoptive cell immunotherapy on ovarian carcinoma. Methods Tumor soluble antigen (TSA) was extracted from cells of ovarian carcinoma (COC 1 and COC 2n ). To generate cytotoxic T lymphocytes (CTL), peripheral blood mononuclear cells (PBMC) collected from healthy persons were induced by TSA and anti CD 3 monoclonal antibody (CD 3 McAb). The antitumor effect of CTL on COC 1 cells in vitro was compared with LAK and CD 3 AK, whereas the growth inhibitory effect of CTL on COC 2n transplantation tumors in nude mice was compared with CD 3 AK. Results The cytotoxicity of CTL, CD 3 AK and LAK was 79.4%, 52.1% and 51.7% respectively ( P <0.01). Mean volume of tumor in nude mice on the 9th day after ovarian carcinoma cells transplanted was 44.4±24.2 mm 3 in CTL group, 118.8± 40.0 mm 3 in CD 3 AK group and 443.0±158.7 mm 3 in control (no treatment) ( P <0.01), whereas mean duration of survival was 28.5 days, 25.5 days and 17 days respectively ( P <0.01). Conclusion The results indicated that CTL exhibited higher cytotoxic effect in vitro and greater inhibitory effect on tumor growth resulting in longer survival in vivo. It suggested that the adoptive cell immunotherapy might provide a new idea for treatment of ovarian carcinoma.

目的探讨卵巢癌过继细胞免疫治疗新方法。方法提取卵巢癌细胞(COC1和COC2n)可溶性抗原(TSA),用TSA和抗CD3单克隆抗体(CD3McAb)共同诱导正常人外周血单个核细胞(PBMC)产生细胞毒性T细胞(CTL),用CTL于体外杀伤COC1细胞和裸鼠体内抑制COC2n移植瘤的生长,体外和淋巴因子激活杀伤细胞(LAK)、淋巴因子和抗CD3单抗激活的杀伤细胞(CD3AK)细胞进行比较,体内和CD3AK细胞进行比较。结果CTL、CD3AK和LAK对COC1细胞的细胞毒作用分别为79.4%、52.1%和51.7%(P<0.01)。在体内,CTL与CD3AK和未经处理的对照组比较,卵巢癌细胞移植到裸鼠体内的第9天,肿瘤平均体积分别为44.4±24.2mm3、118.8±40.0mm3和443.0±158.7mm3(P<0.01),裸鼠平均生存期分别为28.5天、25.5天和17天(P<0.01)。结论本方法诱导产生的CTL,在体内、体外对卵巢癌细胞均有较高的细胞毒作用,能明显抑制肿瘤生长,为卵巢癌治疗提供了新的思路。

The effect of bifidobacterium adolescentis on the ultrastructure of transplantation tumors was observed depending on the animal model of nude mouse transplantation tumor of colorectal cancer.The results showed that this bacteria could induce apoptosis of tumor cells when injected into the peritoneum of tumor-bearing mouses. In early phase,its gross features of apoptotic cells present mainly nuclear chromatin condensation;In intermediate phase,it included mainly the cleavage of nuclear membrane and...

The effect of bifidobacterium adolescentis on the ultrastructure of transplantation tumors was observed depending on the animal model of nude mouse transplantation tumor of colorectal cancer.The results showed that this bacteria could induce apoptosis of tumor cells when injected into the peritoneum of tumor-bearing mouses. In early phase,its gross features of apoptotic cells present mainly nuclear chromatin condensation;In intermediate phase,it included mainly the cleavage of nuclear membrane and nuclei and the formation of apopotic bodies;In late phase,the apoptotic cells were phagocytized mainly by adjacent living cells. The results suggested that the programmed cell death of colorectal cancer induced by this bacteria may be one way of its inhibiting tumors.

本文以大肠癌裸鼠移植瘤为动物模型,用透射电镜观察了青春型双歧杆菌对移植瘤超微结构的影响。结果表明:双歧杆菌经荷瘤鼠腹腔注射后能诱导多量大肠癌细胞凋亡,凋亡早期的细胞主要表现为核染色质浓缩边聚;中期为核膜破裂,胞核崩解,凋亡小体形成;晚期主要为邻近活细胞吞噬凋亡小体。提示双歧杆菌诱导大肠癌细胞程序化死亡可能是其抑瘤机制之一。

 
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