The results showed that the feed with additive of 0.0%~2.5% PSB could improve the activity of alkaline phosphatase(AKP),acidic phosphatase(ACP) and superoxide dismutase(SOD) in liver and serum of grass carps.
Specific activities of protease(P),lipase(L),amylase(A) and cellulase(C) of Babylonia areolata Link were measured respectively at 6 different stages from early veliger to juvenile 15 days after metamorphosis. The activity of protease was found to decrease gradually over time,while the activity of lipase was detected to increase step by step with larval development.
P. stimpsoni has a lethal water temperature at below 11℃,the benumbed condition at 12℃~13℃, the dull condition at 14℃~15℃,commencement of feedding activity at 16℃~17℃,the usual condition for physiological activity at 18℃~30℃, easy catching disease at above 31℃.
After mixed with diluent at the ratio of 1∶2, 1∶5, 1∶10 or 1∶20, sperms of Pinctada martensii were frozen at 4 ℃ for 30 or 60 min, then at -18 ℃ for 4, 24 or 72 h. When the sperms were thawed at room temperature, their activity and fertilizing capability of the sperms were checked.
Epithelial cells of tilapia (Oreochromis nilotica) were isolated and primarily cultured as the cells model to research the effect of PSB0201, Rhodopseudomonas palustris, treated as probiotic, on the morphologic characters, viability, livability, permeability, cytosolic free calcium and membranous phospholipase A2 (PLA2) activity of tilapia epithelial cells.
LitoPenaeus vannamei were fed with formulated diet which had been added with Chinese Traditional Medicines(CTMs) at different contents of 0.0, 0.5, 1.0, 2.0 and 4.0 g·kg-1 respectively. Growth indexs and activity of digestive enzymes were determined in 58 d.
And positive bivalent alkaline earth metalions(Mg2+,Ca2+ and Ba2+)affected the enzyme and the order of the activation intensity was as follows:Mg2+>Ca2+,but Ba2+ inhibit the enzyme activity. The positive trivalent metal ions Al3+ had the inhibitory activity,but the effect was unobvious,and Fe3+ had the activation effect.