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methods applying
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    Methods Applying RT-PCR technique to semiquantitatively analyze CD40 and CD40L mRNA expression in PBMC from 34 SLE patients.
    方法应用逆转录-聚合酶链反应( RT-PCR)检测 34例 SLE患者外周血单一核细胞( PBMC)中 CD40和 CD40配体 mRNA的表达水平。
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    Methods Applying reverse transcription-polymerase chain reaction (RT-PCR) technique to semiquantitatively analyze cPLA2, 5-LO and LTC4-S mRNA expression in the peripheral polymorphonuclear leukocytes (PMNs) from 28 active SLE patients and 25 healthy subjects.
    方法应用反转录聚合酶链反应(RT-PCR)检测了28例活动期SLE患者和25名正常人PMNs中cPLA2、5-LO和LTC4-SmRNA的表达水平。
短句来源
    Methods:Applying reverse transcription-polymerase chain reaction(RT-PCR)Technique to semiquantitatively analyze 5-LO mRNA expression in the peripheral Polymorphomuclear leukocytes(PMNs) from 40 SLE patients and 20 healthy subjects.
    方法:应用反转录聚合酶链反应(RT-PCR)检测了40例活动期SLE患者和20名正常人PMNS中5-LO的表达水平。
短句来源
    Methods:Applying reverse transcription polymerase reactin(RT PCR)technique to semiquantitatively analyze CD28?
    方法 :应用逆转录 聚合酶链反应 (RT PCR)检测 35例活动期SLE患者和 30例正常人外周血单个核细胞 (PBMC)中CD2 8、B7 1和B7 2mRNA的表达水平。
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    Methods Applying the monitoring data of iodine deficiency disorders in the whole country and ChongQing.
    方法应用全国碘缺乏病监测资料及重庆市有关调查资料进行分析。
  “methods applying”译为未确定词的双语例句
    Methods:Applying ELISA technique to analyse the IL-2,IL-6 and anti-dsDNA in the serum from 40 SLE patients.
    方法:用酶联免疫吸附试验(ELISA)检测40例SLE患者血清中IL-2、IL-6和抗ds-DNA抗体水平。
短句来源
    Methods Applying RT- PCR technique to semiquantitatively analyse the expression of IL- 2/IL- 2R in PBMCs from 17 cases of SLE patients and 10 normal controls.
    方法:用逆转录—多聚酶链反应(RT—PCR)法检测了17例SLE患者和10例正常人外周血单个核细胞(PBMC)中IL—2、IL—2RmRNA表达的水平。
短句来源
    Methods:Applying RT - PCR technique to semiquantitatively analyse the mRNA expression of IL- 6 and TNF - α in the PBMCs from 10 normal controls and 15 SLE patients.
    方法:用逆转录-多聚酶链反应(RT-PCR)法检测了10例正常人和15例活动期SLE患者外周血单个核细胞(PBMC)中白介素6(IL-6)和肿瘤坏死因子α(TNF-α)mRNA表达的水平。
短句来源
    Methods Applying RT PCR technique to semiquantitatively analyse the mRNA expression of IL 6 and IL 10 in the PBMCs from 10 normal controls and 15 SLE patients.
    方法 用逆转录多聚酶链反应( RTPCR) 法检测了10 例正常人和15 例活动期SLE 患者外周血单个核细胞(PBMC) 中白介素6(IL6) 和白介素10(IL10) m RNA 表达的水平。
短句来源
    Methods Applying reverse transcriptase polymerase chain reaction (RT PCR) to semiquantitatively analyse the enterovirus RNA and using neutralization test to detect the neutralizing antibody in peripheral blood from 16 PM/DM patients and 10 normal individuals.
    方法 用逆转录聚合酶链反应 (RT PCR)和中和试验分别检测 16例 PM/DM患者和 10例正常人对照组外周血中肠道病毒核糖核酸 (RNA)和抗体滴度。
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  methods applying
The Evolution Path for Industrial Software Quality Evaluation Methods Applying ISO/IEC 9126:2001 Quality Model: Example of MITRE
      
The method is not only simple and reliable, but also has a sensitivity comparable to those methods applying radioactively labelled substrates.
      
Discretization methods for the continuous adjoint are discussed and compared with methods applying the application of the discrete adjoint.
      
Anoverview of the methods applying othermeta-heuristic algorithms including simulatedannealing, tabu search, and artificial neuralnetworks is also given.
      
Evaluation of correlation methods applying neural networks
      
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Objective To investigate the expression of IL - 2/IL - 2R mRNA expression in SLE patients. Methods Applying RT- PCR technique to semiquantitatively analyse the expression of IL- 2/IL- 2R in PBMCs from 17 cases of SLE patients and 10 normal controls. Results Compared with normal controls, the IL - 2 mRNA expression in SLE PBMCs was significantly decreased (P< 0.01) ; whereas, the IL - 2R mRNA expression was markedly increased(P<0.01).Conclusion The production of IL-2R in SLE patients is not induced by 1L-2....

Objective To investigate the expression of IL - 2/IL - 2R mRNA expression in SLE patients. Methods Applying RT- PCR technique to semiquantitatively analyse the expression of IL- 2/IL- 2R in PBMCs from 17 cases of SLE patients and 10 normal controls. Results Compared with normal controls, the IL - 2 mRNA expression in SLE PBMCs was significantly decreased (P< 0.01) ; whereas, the IL - 2R mRNA expression was markedly increased(P<0.01).Conclusion The production of IL-2R in SLE patients is not induced by 1L-2.

目的:探讨系统性红斑狼疮(SLE)患者中白介素2(IL—2)及其受体(IL—2R)表达的自然情况。方法:用逆转录—多聚酶链反应(RT—PCR)法检测了17例SLE患者和10例正常人外周血单个核细胞(PBMC)中IL—2、IL—2RmRNA表达的水平。结果:与正常人相比,活动期SLE患者IL—2mRNA表达水平降低而IL—2mRNA表达水平增高(P<0.01)。结论:SLE患者中IL—2R的表达未受IL—2的诱导。

Objective: To investigate some inflammatory - mediatable cytokine expresions in SLE patien ts. Methods:Applying RT - PCR technique to semiquantitatively analyse the mRNA expression of IL- 6 and TNF - α in the PBMCs from 10 normal controls and 15 SLE patients. Results: Compared with normal controls, the mRNA expression of IL - 6 and TNF - α in SLE PBMCs were significantly increased (p < 0.01, both). Conclusion: IL - 6 and TNF - α in active SLE patients is up-regulated, which may play a certain role...

Objective: To investigate some inflammatory - mediatable cytokine expresions in SLE patien ts. Methods:Applying RT - PCR technique to semiquantitatively analyse the mRNA expression of IL- 6 and TNF - α in the PBMCs from 10 normal controls and 15 SLE patients. Results: Compared with normal controls, the mRNA expression of IL - 6 and TNF - α in SLE PBMCs were significantly increased (p < 0.01, both). Conclusion: IL - 6 and TNF - α in active SLE patients is up-regulated, which may play a certain role in the inflammatory reactions of SLE patients.

目的:探讨系统性红斑狼疮(SLE)中某些具炎症介质作用细胞因子的自然表达。方法:用逆转录-多聚酶链反应(RT-PCR)法检测了10例正常人和15例活动期SLE患者外周血单个核细胞(PBMC)中白介素6(IL-6)和肿瘤坏死因子α(TNF-α)mRNA表达的水平。结果:与正常人相比,活动期SLE患者PBMC中IL-6和TNF-αmRNA的表达水平均增高(均P<0.01)。结论:SLE患者中IL-6与TNF-α的表达上调,可能在发病机制中起一定作用。

Objective To investigate the mRNA expression of Th1 type cytokines in SLE patients Methods Applying RT-PCR technique to semiquantitatively analyse the IL 2 mRNA expression in PBMCs from 41 SLE patients and IFN γ mRNA expression in PBMCs from 31 SLE patients Results Compared with normal controls, the SLE patients with decreased expression of IL 2 accounted for 78 0% of the SLE patients in active stage,and those with decreased expression of IFN γ accounted for 83 9%, the mean levels...

Objective To investigate the mRNA expression of Th1 type cytokines in SLE patients Methods Applying RT-PCR technique to semiquantitatively analyse the IL 2 mRNA expression in PBMCs from 41 SLE patients and IFN γ mRNA expression in PBMCs from 31 SLE patients Results Compared with normal controls, the SLE patients with decreased expression of IL 2 accounted for 78 0% of the SLE patients in active stage,and those with decreased expression of IFN γ accounted for 83 9%, the mean levels of IL 2 and IFN γ expression in SLE group were significantly decreased as well (P< 0 01,both) Conclusion The Th1 type cytokine expression of SLE patients in active stage is down regulated,which may play a role in the pathogenesis of SLE

目的探讨系统性红斑狼疮(SLE)患者中Th1型细胞因子表达的情况。方法用逆转录 聚合酶链反应(RT PCR)法检测了SLE患者外周血单一核细胞(PBMC)中白介素2(IL 2)mRNA表达(41例)和γ干扰素(IFN γ)mRNA表达(31例)的水平。结果与正常人相比 ,活动期SLE患者中IL 2表达水平降低者占78 0 % ,IFN γ表达水平降低者占83 9 %。活动期SLE组PBMC中IL 2和IFN γmR NA表达的平均水平也降低(P均<0 01)。结论SLE患者中Th1型细胞因子的表达下调 ,可能在发病机制中起一定作用

 
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