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rescue assay
相关语句
  补救分析
     While the marker rescue assay can detect RCR with a limit at 6×102CFU/ml, RT/PCR can be used to detect RCR at as low as lCFU/103ml.
     补救分析可检测到6×10~2CFU/ml(Colony Forming Units/ml)的有复制能力的逆转录病毒,RT/PCR的灵敏度为1CFU/10~3ml。
短句来源
     In this study, two methods, namely the marker rescue assay and RT/PCR, were employed to detect RCR in the cells.
     本实验运用了Marker Rescue Assay(补救分析)和RT/PCR(逆转录PCR)两种方法。
短句来源
     Methods The methods used included polymerasechain reaction (PCR), reverse transcripted PCR (RT/PCR), Southern blot, rescue assay of Neo andlacZ gene, cell implantation, PCR for HSV-tk, X-gal staining and histomicrosection.
     方法DNA聚合酶链式反应(PCR)、反转录PCR(RT/PCR),Southern杂交及neo基因和LacZ基因补救分析:细胞接种、X-gal染色、TK基因PCR和病理切片检查等。
短句来源
     S +/L - assay, NIH3T3 amplification and rescue assay of neo gene were performed to determine RCR.
     支原体检测采用聚合酶链反应(PCR)方法,可复制性逆转录病毒的检测应用S+/L-试验,NIH3T3细胞扩增试验和neo基因补救分析
短句来源
     The amphotropic viruses in supernatants were titrated to 1 3×10 5CFU/ml. No helper virus could be found by both nested PCR and rescue assay.
     双嗜型病毒生产细胞的滴度为 1 3× 10 5CFU/ml,巢式PCR和补救分析均未检测到辅助病毒存在 ;
短句来源
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  “rescue assay”译为未确定词的双语例句
     In this study, we used S+L- assay, marker rescue assay and PCR to detect RCR after 3T3 amplification.
     本实验对经3T3扩增的样品进行 S~+L~-分析、标记拯救分析和PCR扩增方法检测RCR。
短句来源
     First, polymerase chain reaction(PCR), reverse transcripted PCR(RT/PCR), Southern blot and rescue assay were used to detect replication competent retrovirus (RCR) in HSV TK virus producing cell line (TK/VPC), C6TK and SHGTK genetically modified cell and blood samples.
     首先采用PCR、反转录PCR(RT/PCR)、Southern杂交以及补救分析方法,对HSV-tk病毒包装细胞(TK/VPC)、C6转TK基因细胞和实验动物血液进行了检测。
短句来源
     Comparing the three methods, We found that the marker rescue assay was easy-manipulated and PCR was sensitive capable of detecting one marked cell among 105 unmarked cells.
     比较三种方法,标记拯救分析比S~+L~-分析结果容易判断,且PCR可以从10~5个无病毒基因的细胞中检测出一个带有病毒基因的细胞。
短句来源
     Retrovirus in the supernatant was titrated to 6.2×10~5CPU/ml for GP+E86/HaMDR pllos and 8. 5×105CFU/ml for PA317HaMDR pools respectively. No wild-type helper virus was found by rescue assay and/or nested polymerase chain reaction (PCR).
     结果:鼠源单向型与双嗜型病毒生产细胞的滴度分别为6.2×105CF/ml和8.5×105CFU/ml,无辅助病毒产生;
短句来源
  相似匹配句对
     Rescue the Earth
     拯救地球
短句来源
     Rescue OTCBB
     拯救三板市场
短句来源
     RADIOENZYMATIC ASSAY OF NORADRENALINE
     血浆去甲肾上腺素的放射酶测定法
短句来源
     IMPROVEMENT ON THE ASSAY FOR CEFOPERAZONE
     头孢哌酮含量测定用流动相的改进
短句来源
     Nullification assay was used to examine whether EGCG nullify the rescue effect of deoxycytidine (dCdR) to AraC.
     以对消实验研究EGCG能否逆转脱氧胞苷 (dCdR)的补救作用 ;
短句来源
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  rescue assay
In marker rescue assay, Splt-p49 was able to suppress apoptosis induced by infection of a mutant AcMNPV deficient in p35 and rescue the mutant virus replication from apoptosis in Sf-9 cells.
      
The mutant protein is inactive in a zebrafish rescue assay, indicating a role for TDGF1 in human midline and forebrain development.
      
We are left to conclude that an intactcell rescue assay provides a more stringent test of transcriptional function.
      
The plasmid rescue assay shuttles recircularizes BAC plasmid from the infected U87 cells back into the bacterial host.
      
The ability of hybrid ITR vectors to form circular intermediates was evaluated by Hirt Southern blots and a bacterial rescue assay.
      
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This paper deals with safety assessments required for animal test and clinical trial in haemophilia B gene therapy. Based on ourprevious works on gene transfer therapy for hemophilia B, a series of safety assessments were complered before clinical trial. Firstly, the existence of replication-completent retrovirus (RCR)were sensitively detected by Neo gene and lac Z gene rescue assay on the cellular level, and PCR, RT/PCR with Southern blot and dot blot on DNA and RNA level,results were found to be negative....

This paper deals with safety assessments required for animal test and clinical trial in haemophilia B gene therapy. Based on ourprevious works on gene transfer therapy for hemophilia B, a series of safety assessments were complered before clinical trial. Firstly, the existence of replication-completent retrovirus (RCR)were sensitively detected by Neo gene and lac Z gene rescue assay on the cellular level, and PCR, RT/PCR with Southern blot and dot blot on DNA and RNA level,results were found to be negative. Secondly, malignant transformation was followed on cells transduced with F ac cDNA by various techipues including cell morphology, histochemistry, chromsome karyotyping, soft-agar test, nude mice test and electromicroscopy, no abnormal changes were observed. Lastly, presence of bacteria, mold, endotoxin, toxin, allergen was rotinely done for culture medium and collagen and others used in clinical trial. All these safety assessments demonstrate that the safety of implantion of transduced cells is guaranteed.

报道了血友病B基因治疗临床Ⅰ期试验的安全性研究,包括反转录病毒基因的DNA聚合酶链式反应(PCR)、反转录PCR(RT/PCR)、Southern杂交以及neo基因和lacZ基因的补救分析(resoueassay).从DNA水平。RNA水平和病毒活体水平对野生型病毒进行了检测,没有检测到反转录病毒,并对兔和人转基因细胞进行形态学观察、染色体核型分析、软琼脂实验、裸鼠接种实验、兔和裸鼠的病理检测及电镜分析以检测恶性细胞的存在与否,同时进行支原体检测、内毒素和过敏原测定,结果均为阴性.建立了有复制能力的野生型反转录病毒(RCR)的检测系统,为基因治疗临床试验的安全性提供了保证.

Retroviral vectors are the most used gene delivery vehicle in human gene therapy.When the therapeutic gene is packagedfrom producer cell line, one has to determined whether thereplication-competent retrovirus(RCR) is being generated. In this study, two methods, namely the marker rescue assay and RT/PCR, were employed to detect RCR in the cells. While the marker rescue assay can detect RCR with a limit at 6×102CFU/ml, RT/PCR can be used to detect RCR at as low as lCFU/103ml. Combining the specificity...

Retroviral vectors are the most used gene delivery vehicle in human gene therapy.When the therapeutic gene is packagedfrom producer cell line, one has to determined whether thereplication-competent retrovirus(RCR) is being generated. In this study, two methods, namely the marker rescue assay and RT/PCR, were employed to detect RCR in the cells. While the marker rescue assay can detect RCR with a limit at 6×102CFU/ml, RT/PCR can be used to detect RCR at as low as lCFU/103ml. Combining the specificity of the marker rescue assay and the sensitivity of RT/PCR, both assays together should serve as an adequate test for detecting the generation of RCR in retroviral producer cell lines.

运用逆转录病毒作为外源基因导入的载体,在实际临床运用时需要检测包装细胞在表达目的基因的同时,是否会产生有复制能力的逆转录病毒。本实验运用了Marker Rescue Assay(补救分析)和RT/PCR(逆转录PCR)两种方法。补救分析可检测到6×10~2CFU/ml(Colony Forming Units/ml)的有复制能力的逆转录病毒,RT/PCR的灵敏度为1CFU/10~3ml。这两种检测方法的建立,为逆转录病毒载体用于临床基因治疗的安全性提供一定的保证。

Replication of competent retrovirus(RCR) is the primary danger in retro-viral-mediated gene transfer involved gene therapy. In this study, we used S+L- assay, marker rescue assay and PCR to detect RCR after 3T3 amplification. Comparing the three methods, We found that the marker rescue assay was easy-manipulated and PCR was sensitive capable of detecting one marked cell among 105 unmarked cells. Since safety must be first considered before clinical trials of human gene therapy, the three assays...

Replication of competent retrovirus(RCR) is the primary danger in retro-viral-mediated gene transfer involved gene therapy. In this study, we used S+L- assay, marker rescue assay and PCR to detect RCR after 3T3 amplification. Comparing the three methods, We found that the marker rescue assay was easy-manipulated and PCR was sensitive capable of detecting one marked cell among 105 unmarked cells. Since safety must be first considered before clinical trials of human gene therapy, the three assays could be useful for monitoring against RCR in retroviral-mediated gene therapy.

逆转录病毒介导的基因治疗中安全性的最大危险是产生有复制能力的逆转录病毒(RCR)。本实验对经3T3扩增的样品进行 S~+L~-分析、标记拯救分析和PCR扩增方法检测RCR。比较三种方法,标记拯救分析比S~+L~-分析结果容易判断,且PCR可以从10~5个无病毒基因的细胞中检测出一个带有病毒基因的细胞。3T3扩增可提高灵敏度约10倍。安全性是基因治疗首要考虑的问题,本方法为其提供了保证。

 
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