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nerve tube
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  神经管
    Whole-mount in situ hybridization showed that mRNA level of Bsg6 in mouse embryos was vary in different regions of brain. Expression was found in brain and nerve tube of E8.5 mouse embryo.
    Bsg6在小鼠胚胎中的表达具有一定动态性,在E8.5的小鼠胚胎中,Bsg6主要在前脑和神经管表达.
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    Bsg6 expression was also found in brain and anterior of nerve tube of HH10 chicken embryo.
    在HH10期的鸡胚中,Bsg6主要在头部和神经管前端表达.
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    Noggin induces the nerve tube formation.
    在神经系统中诱导神经管的形成;
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    EP was recorded from base turn of the cochlea, AP in response to theclicks of 105 dBpe SPL was recorded with the electrode in the facial nerve tube, its amplitude ofwave negative one (N1) was expressed by the percentage compared to its pre-administration level.
    EP由耳蜗基底周引出,AP以105dBpeSPL短声诱发,面神经管电极记录. 对AP-N1振幅百分比与EP值进行相关性分析.
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    Immunocytochemical localization were investigated for the first time in the gonads, brain vesicle and nerve tube of Amphioxus using polyclonal antibodies against 17β-estradiol, testosterone and progesterone receptors.
    首次用雌二醇、睾酮和孕酮受体的多克隆抗体在文昌鱼性腺、脑泡和神经管中进行免疫细胞化学定位研究。
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  “nerve tube”译为未确定词的双语例句
    THE STUDY ON THE EFFECTS OF NERVE GROUTH FACTOR AND ITS RECEPTOR ON THE NERVE TUBE DEFECT INDUCED BY EXCESS RETINOIC ACID
    神经生长因子及其受体在过量维生素A酸所致神经管畸形发生中的作用
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  nerve tube
In addition, some immunopositive cells existed in the most proximal part of the caudal nerve tube and may be motoneurons.
      
These axon bundles joined in the posterior sensory vesicle, ran posteriorly through the visceral ganglion and branched into two caudal nerves which ran along the lateral walls of the caudal nerve tube.
      
It is found for the first time that the immunoreactivity of GnRHR exists in the nerve cells and fibers in the amphioxus's brain and nerve tube and the epithelial cells of Hatschek's pit at the different stages of gonadal development.
      
Because the nerve tube wall and the basement membraneremain intact, they are able to containthese tissues.
      


Atstract The purpose of the present study is to investigate the correlation between the com-pound action potential (AP) and the endocochlear potential (EP), in order to further understandthe electrophysiological and pathophysiological mechanism in cochlea. EP and AP were measuredsimultaneously from the guinea pigs administered the mixture of furosemind and bovine serum al-bumin by the jugular vein. EP was recorded from base turn of the cochlea, AP in response to theclicks of 105 dBpe SPL was recorded with the...

Atstract The purpose of the present study is to investigate the correlation between the com-pound action potential (AP) and the endocochlear potential (EP), in order to further understandthe electrophysiological and pathophysiological mechanism in cochlea. EP and AP were measuredsimultaneously from the guinea pigs administered the mixture of furosemind and bovine serum al-bumin by the jugular vein. EP was recorded from base turn of the cochlea, AP in response to theclicks of 105 dBpe SPL was recorded with the electrode in the facial nerve tube, its amplitude ofwave negative one (N1) was expressed by the percentage compared to its pre-administration level.The results indicated that the correlation between the magnitude of EP and the amplitude percent-age of AP distributed as "S" type curve, the amplitude percentage of AP was strongly and posi-tively correlated with the magnitude of EP (r>0. 9). As EP was over 60~70 mV, the amplitudeof AP could keep over 80%; as EP reduced from 60 mV to 40 mV, or rose from 30 mV to 70 mV,AP changed remarkmably; as EP was less than 30 mV, AP would near zero. This study suggestedthat the correlation between AP and EP was typical type "S", and the amplitude of AP is stronglyand positively correlated with and dependent on the magnitude of the EP.

目的:探讨听神经动作电位(AP)与耳蜗内电位(EP)之间的关系,为进一步研究内耳电生理和病理机制提供依据.方法:经豚鼠颈静脉注入速尿与白蛋白混合液,同步记录EP和AP.EP由耳蜗基底周引出,AP以105dBpeSPL短声诱发,面神经管电极记录.对AP-N1振幅百分比与EP值进行相关性分析.结果:AP振幅百分比与EP幅值关系呈明确的“S”型分布,相关性分析表明两者为高度的正相关(r>0.9).当EP值在60~70mV以上时,AP-N1振幅能保持在80%以上;当EP值从60mV降至40mV,或从30mV上升至70mV时,AP-N1振幅变化急剧;当EP值低于30mV时,AP则趋于消失.结论:AP和EP呈“S”型的对应关系,AP的振幅高度正相关于并依赖于EP幅值.

Objective To fabricate a Schwann cell seeded intrinsic framework for a tissue engineered bioartificial nerve Methods With a silicon ring, a three dimensional Vicryl  or PDS  fiber net was constructed and coated with Matrigel, and 10 6/ml cultured newborn or adult rat Schwann cells were incubated...

Objective To fabricate a Schwann cell seeded intrinsic framework for a tissue engineered bioartificial nerve Methods With a silicon ring, a three dimensional Vicryl  or PDS  fiber net was constructed and coated with Matrigel, and 10 6/ml cultured newborn or adult rat Schwann cells were incubated with this fiber net. A live/dead fluorescent staining and a scanning electron microscopic observation of cell adhesion on the fiber surface were performed. As a preliminary in vivo experiment, 1.5 cm long collagen nerve tubes implanted with ten Matrigel coated Vicryl  or PDS  fibers were employed to bridge the sciatic nerve defects in Lewis rats. Five weeks later, histological and transmission electron microscopic observation were made. Results Schwann cells attached and extended longitudinally onto the fiber net. Several cell chains were observed to encircle the fibers. The fluorescent staining demonstrated the longitudinally arranged viable cell columns, even in a multilayer overlapping fashion. After five week in vivo implantation, well vascularized connective tissues replaced the surface of the collagen nerve tubes, which contained matrigel coated fibers. It was demonstrated that the regenerated nerve fascicles grew along the fibers and traversed the middle point of the tubes. Conclusions The growth of Schwann cells onto the intrinsic framework was similar to the “Bungner bands” formed during Wallerian degeneration. Therefore, it can be considered as an appropriate intrinsic framework for the bioartificial nerve. Based on the preliminary in vivo experiment of the collagen nerve tubes with fibers, we concluded that the materials for the fabrication of a bioartificial nerve had a good biological compatibility.

目的 研制组织工程化人工神经内生长雪旺细胞的内部支架结构。方法 用 6 0的生物可吸收Vicryl 或PDS 纤维 ,在硅胶圈上编织成三维立体网架 ,经Matrigel蛋白胶涂层 ,与 10 6/ml新生或成年鼠雪旺细胞共同培养 3d。Live/Dead荧光染色和扫描电镜观察细胞贴附情况。将 10根经Matrigel蛋白胶涂层的Vicryl 或PDS 纤维 ,植入 1.5cm长的胶原神经管内 ,然后将其桥接于Lewis鼠坐骨神经缺损处。术后 5周取材作组织学和透射电镜观察。结果 在三维立体网架上 ,雪旺细胞沿纤维纵轴贴附伸展 ,并围绕纤维形成多条细胞链。荧光染色显示在纤维上有纵行的活细胞带 ,并见到多层重叠现象。组织工程化人工神经在体内 5周 ,含有涂层纤维的胶原神经管表面为有丰富的新生血管的软组织所取代。神经束已沿生物纤维越过神经管中点。结论 培养的雪旺细胞在纤维网架上贴附生长 ,类似瓦勒变性后形成的“Bungner”带 ,被认为是合适的人工神经内部支架结构。实验结果表明 ,种植纤维的胶原神经管体 ,为构筑人工神经所采用的具有良好生物相容性的材料

Immunocytochemical localization were investigated for the first time in the gonads, brain vesicle and nerve tube of Amphioxus using polyclonal antibodies against 17β-estradiol, testosterone and progesterone receptors. The results indicated that the receptors of estrogen, androgen and progestogen existed in oogonia and oocytes of different developmental stages. At the small growth stage, three kinds of the sex steroid receptors usually were localized in the cytoplasm or nuclear membrane of oogonia and early...

Immunocytochemical localization were investigated for the first time in the gonads, brain vesicle and nerve tube of Amphioxus using polyclonal antibodies against 17β-estradiol, testosterone and progesterone receptors. The results indicated that the receptors of estrogen, androgen and progestogen existed in oogonia and oocytes of different developmental stages. At the small growth stage, three kinds of the sex steroid receptors usually were localized in the cytoplasm or nuclear membrane of oogonia and early primary oocyte, and localized in the cytoplasm and nucleoplasm of oocytes at the large growth stage and mature stage. In male, three kinds of sex steroid receptors were distributed in the spermatogonia, spermatocytes and spermatids (except estrogen receptor), and the positive reaction (substance) was localized in the cytoplasm and nucleus of these spermatogenic cells. In addition, the gonads at different stages and nervous system in amphioxus were also investigated by using immunocytochemical method and sex steroid hormones (17β-estradiol, testosterone and progesterone) antibodies. The results showed that immunopositive substance existed in the ovary and testis of different stages. At the early developmental stage of ovary, the cytoplasm and nucleolar membrane of oogonia and early primary oocyte showed immunopositive reaction with strong or medium intensity to 17β-estradiol, testosterone and progesterone antibodies. At the large growth stage and mature stage, immunopositive reaction to testosterone and progesterone antibodies in cytoplasm and nucleolar membrane of oocyte weakened obviously, but the immunopositive reaction to 17β-estradiol antibodies strenthened notably. In male, the immunopositive reaction in spermatogonia, spermatocyte and spermatid to testosterone antibodies strengthened gradually during the development and maturation of testis. At large growth stage and mature stage, the spermatogonia and spermatocyte showed strong immunopositive reaction to progesterone antibodies, but immunonegative reaction to 17β-estradiol antibodies at the mature stage. In sperm, the reaction was negative. On the other hand, we found for the first time that mammalian neurosteroids-like immunopositive nerve cell and its fiber existed in the brain vesicle and nerve tube of Amphioxus, and the positive substance distributed in the cytoplasm of nerve cell, while nucleus showed negative reaction. These results provide a new morphological proof that sex steroid hormones participate in the regulation of gonadal development and functional maturation of reproductive endocrine control axis in Amphioxus.

首次用雌二醇、睾酮和孕酮受体的多克隆抗体在文昌鱼性腺、脑泡和神经管中进行免疫细胞化学定位研究。结果表明 ,不同发育时期的卵原细胞和卵母细胞中都存在雌激素、雄激素和孕激素受体。在小生长期 ,3种受体通常定位在卵原细胞和早期初级卵母细胞的胞质或核膜 ;在大生长期和成熟期 ,则定位在胞质和核质。在雄性 ,3种类固醇激素受体分布在不同发育时期的精原细胞、精母细胞和精子细胞 (雌激素受体例外 )中 ,受体阳性物定位在胞质和核。另外 ,用免疫细胞化学方法和性类固醇激素抗体对文昌鱼不同发育时期的性腺和神经系统进行研究 ,结果揭示 ,免疫阳性物存在于不同发育时期的卵巢和精巢中。在卵巢发育早期 ,卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应 ;在大生长期和成熟期 ,卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱 ,而对雌二醇则显著增强。在雄性 ,随着精巢发育成熟 ,精原细胞、精母细胞和精子细胞对睾酮抗体的免疫阳性反应逐渐增强。在大生长期和成熟期 ,精原细胞和精母细胞对孕酮抗体发生强的免疫阳性反应。在成熟期则对雌二醇抗体发生免疫阴性反应。精子始终为免疫阴性。本文研究还首次发现 ...

首次用雌二醇、睾酮和孕酮受体的多克隆抗体在文昌鱼性腺、脑泡和神经管中进行免疫细胞化学定位研究。结果表明 ,不同发育时期的卵原细胞和卵母细胞中都存在雌激素、雄激素和孕激素受体。在小生长期 ,3种受体通常定位在卵原细胞和早期初级卵母细胞的胞质或核膜 ;在大生长期和成熟期 ,则定位在胞质和核质。在雄性 ,3种类固醇激素受体分布在不同发育时期的精原细胞、精母细胞和精子细胞 (雌激素受体例外 )中 ,受体阳性物定位在胞质和核。另外 ,用免疫细胞化学方法和性类固醇激素抗体对文昌鱼不同发育时期的性腺和神经系统进行研究 ,结果揭示 ,免疫阳性物存在于不同发育时期的卵巢和精巢中。在卵巢发育早期 ,卵原细胞和卵母细胞的胞质和核仁膜对雌二醇、睾酮和孕酮抗体显示强或中等强度免疫阳性反应 ;在大生长期和成熟期 ,卵母细胞胞质和核仁膜对睾酮和孕酮抗体的免疫阳性反应明显减弱 ,而对雌二醇则显著增强。在雄性 ,随着精巢发育成熟 ,精原细胞、精母细胞和精子细胞对睾酮抗体的免疫阳性反应逐渐增强。在大生长期和成熟期 ,精原细胞和精母细胞对孕酮抗体发生强的免疫阳性反应。在成熟期则对雌二醇抗体发生免疫阴性反应。精子始终为免疫阴性。本文研究还首次发现 ,文昌鱼脑泡和神经管中存在哺乳动物神经?

 
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