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genetically modified
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  转基因
     Identification for Genetically Modified Maize T14/T25 with Real Time Fluorenscent PCR Method
     用实时荧光PCR方法鉴定转基因玉米T14/T25
短句来源
     Establishment of the Real |time Fluorescent PCR Detection Method for Genetically Modified GA21 Maize
     GA21转基因玉米实时荧光PCR检测方法的建立
短句来源
     Crop line differentiating flanking sequences of 6 lines of genetically modified (GM) maize (MON810, BT11, BT176, GA21, T25 and CBH-351) and 1 line of GM soybean GTS 40-3-2, 2 lines of GM canola (RT73 and MS8) and 2 lines of GM cotton (MON531 and MON1445) were known using inverted PCR cloning-sequencing approach to determine unknown sequences.
     本研究采用反向PCR克隆—测序测定未知序列技术,针对转基因玉米(MON810、BT11、BT176、GA21、T25、CBH-351)六个品系、转基因大豆GTS 40-3-2品系、转基因油菜RT73和MS8两个品系、转基因棉花MON531和MON1445两个品系测定出品系鉴定的边界序列;
短句来源
     Fluorescence Quantitative PCR Detection of Genetically Modified Food by TaqMan Probe
     TaqMan探针用于转基因食品的荧光定量PCR检测
短句来源
     Quantitative Detection of Genetically Modified Bt176 Maize in Maize Powder
     玉米粉中转基因Bt176玉米的定量检测
短句来源
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  基因修饰
     Retrograde HRP tracing after transplantation of BDNF genetically modified NSCs at spinal cord injury in rat
     大鼠脊髓损伤BDNF基因修饰神经干细胞移植后HRP逆行示踪
短句来源
     INFLUENCE OF INTRASPINAL IMPLANTATION OF pSVP_0MCAT GENETICALLY MODIFIED SCHWANN CELL IN REGENERATION OF INJURED SPINAL CORD
     pSVP_0Mcat微基因修饰雪旺细胞脊髓内移植对损伤脊髓再生的影响
短句来源
     HHK Artificial Tendon and Genetically Modified Mesenchymal Stem Cells for Tendon Repair
     HHK人工腱和基因修饰的间充质干细胞修复肌腱损伤
短句来源
     Focused on the choice of targeted sequences,the isolation of DNA and the validation of PCR products,this article summarizes the application of PCR (Polymerase Chain Reaction,PCR) in the qualitative detection of GMOs (Genetically modified organisms,GMOs),and then points out existing problems and its future development.
     以靶序列的选择、DNA提取和产物的检测为主线,概括了PCR(PolymeraseChainReaction,PCR)在定性检测基因修饰有机体(Geneticallymodifiedorganisms,GMOs)中的应用,指出了存在的问题以及未来的发展方向。
短句来源
     NT-3 genetically modified Schwann cells promote neural stem cells to differentiate into neuron-like cells
     NT-3基因修饰施万细胞促进神经干细胞体外分化为神经元样细胞
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  “genetically modified”译为未确定词的双语例句
     Reverse Effect of Genetically Modified Adenovirus H101 on Drug-resistance of A549/DDP Cells to Cisplatin
     基因工程腺病毒H101逆转A549/DDP细胞对顺铂耐药性的实验研究
短句来源
     The BM-MSCs genetically modified with pcDNA3.1(+)/NT3 not only expressed and produced NT3, but also promoted the survival of the guinea pig cochlea hair cells in vitro.
     pcDNA3.1(+)/NT3转染BM-MSCs后,能够表达和分泌NT3,并具有生物学活性,可促进离体的豚鼠耳蜗毛细胞存活。
短句来源
     The NT 3 expression of the NT 3 genetically modified SCs(NT 3 SCs) was determined immunocytochemically, and the amount of NT 3 in the cultural media of NT 3 SCs was determined with ELISA method.
     用免疫细胞化学和ELISA方法分别检测NT-3基因转染SCs的NT-3表达及培养液中NT-3的含量。
短句来源
     A Genetically Modified Japonica Restorer Line,C418-Xa21,and Its Hybrid Rice with Bacterial Blight Resistance
     经基因工程改良的抗白叶枯病水稻粳型恢复系“C418-Xa21”及其杂交稻
短句来源
     One of the pathways of the beneficial effects of intracerebral transplantation of genetically modified myoblasts producing BDNF on HIBI lies on the inhibition of the function of dynorphin A 1-13 through the binding of κ opioid receptor.
     植入BDNF载体细胞减轻HIBI的作用途径之一 ,是抑制了强啡肽A1 13 与其特异性阿片κ受体相结合所产生的加重BIHI的效应。
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  genetically modified
Using the induced mutagenesis technique, a series of genetically modified Clavicepssp.
      
Luminescence of intact cells of Escherichia coli lum+ (a genetically modified strain) was measured on exposure to antibiotics, probiotics, phages, and biostimulators.
      
Effect of Wild and Genetically Modified Rhizosphere Bacteria Pseudomonas aureofaciens on the Accumulation of Arsenic by Plants
      
Genetically modified variants of the strain Pseudomonas aureofaciens BS1393 have been constructed that are resistant to high concentrations of arsenic and dissolve poorly soluble phosphates and/or arsenates.
      
Accuracy of a real-time polymerase-chain-reaction assay for a quantitative estimation of genetically modified sources in food pr
      
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Reccent investigations demonstrated that genetically modified tumor cells could be used as vaccines against cancer. In this light, we introduced human interleukin -2 (IL-2) gene into a poorly immunogenic rat fibrosarcoma cell line WBT, a human lung abenocarcinoma cell line AGZY and a highly metastatie sub - cell line Anip973 derived from AGZY. The transduced cells showed no obvious changes in morphology, growth and colony - forming ability in soft agar.Transduction with IL-2 gene rendered the cells more...

Reccent investigations demonstrated that genetically modified tumor cells could be used as vaccines against cancer. In this light, we introduced human interleukin -2 (IL-2) gene into a poorly immunogenic rat fibrosarcoma cell line WBT, a human lung abenocarcinoma cell line AGZY and a highly metastatie sub - cell line Anip973 derived from AGZY. The transduced cells showed no obvious changes in morphology, growth and colony - forming ability in soft agar.Transduction with IL-2 gene rendered the cells more sensitive than the parental cells and cells transduced with NeoR gene alone to the lysis by cytotoxic lymphocytes. High levels of IFN and TNF were detected in the supernatants of cocultured lymphocytes and tumor cells containing IL-2 gene. Tumorigenicity of WBT/IL-2 cells was greatly reduced in syngeneic rats. Metastatic capacity of Anip973 in nude mice was blocked by the transduction of IL-2 gene, but not the transduction of NeoR gene alone. Inoculation of WBT/IL-2 cells conferred rats resistance to the challenge of parental WBT cells.Splenocytes from WBT/IL-2-inoculated rats restimulated with WBT cells in vitro showed high CTL activity against WBT cells. Our results demonstrated the potential application of tumor cells transduced with IL— gene as active vaccine against cancer.

我们应用逆转录病毒载体pLXSN将人IL-2基因插入大鼠和人的肿瘤细胞.探讨了携带外源性IL-2基因的肿瘤细胞的体内外生物学特性及其意义.转基因细胞在体外培养中的细胞形态、生长特性以及软琼脂集落形成能力未见明显改变,但对杀伤性免疫活性细胞的杀伤更为敏感,与淋巴细胞混合培养的上清中测得更高水平的IFN和TNF活性.大鼠肿瘤细胞WBT插入IL-2基因后,其体内成瘤性明显下降.腹腔接种WBT/IL-2细胞可使大鼠获得抗肿瘤免疫能力以抑制皮下野生型WBT细胞的生长.携带IL-2基因的人和大鼠肿瘤细胞在裸鼠体内可以成瘤,但生长缓慢.具有高转移能力的Anip973细胞插入IL-2基因后,在裸鼠体内的转移性丧失.结果提示IL-2基因插入肿瘤细胞进行肿瘤基因治疗的有效性、可行性和发展潜能.

This paper deals with the clinical trial of gene therapy on four hemophilia B patients. Autologous skin fibroblasts from the patients trans feeted by retroviral vector are capable to express human clotting factor Ⅸ protein at a high level in vitro. Such genetically modified fibroblasts were embedded in collagen matrix and then transplanted subcutaneously in the patients. After treatment, bleeding of four patients was alleviated, and both concentration and bio-activity of clotting factor Ⅸ protein increased....

This paper deals with the clinical trial of gene therapy on four hemophilia B patients. Autologous skin fibroblasts from the patients trans feeted by retroviral vector are capable to express human clotting factor Ⅸ protein at a high level in vitro. Such genetically modified fibroblasts were embedded in collagen matrix and then transplanted subcutaneously in the patients. After treatment, bleeding of four patients was alleviated, and both concentration and bio-activity of clotting factor Ⅸ protein increased. In two cases the values reached to 4 %-5 % of the normal level, while the other two also increased in some extnts. 400 days later, one patient was treated again,the concentration and bio-activity of clotting factor Ⅸ protein in his plasma increased again, and remained thus for more than three years. No replication--competent retrovirus, malignant transformed cells and treatment-relative side effects were detected in this trial and follow-up evaluation. The result showed that autologously injection of genetically modified human skin fibroblasts could be a simple and feasible system of treatment on hemophilia B patients.

报道了对四例血友病B患者基因治疗的临床试验,取自四例血友病B患者的自体皮肤成纤维细胞,通过反转录病毒介导的基因转移,能高效地分泌人凝血因子Ⅸ.用胶原包埋经遗传修饰的自体皮肤成纤维细胞,再移植到血友病B患者皮下.四例患者经治疗后,自发出血症状得到了减轻,血浆中凝血因子Ⅸ蛋白浓度和凝血活性明显地增加,其中两例患者增加较多,达到正常值的4%~5%.另外两例患者体内的凝血因子Ⅸ和凝血活性也有不同程度的提高.一例患者400多天后经重复治疗,凝血因子Ⅸ浓度和凝血活性再次升高,持续至今已三年以上,临床治疗时和治疗后的随访中,均没有观察到复制型反转录病毒、恶性转化细胞及其他任何与治疗相关的副作用.试验结果表明:用经遗传修饰的分泌人凝血因子Ⅸ的皮肤成纤维细胞进行自体移植,为基因治疗血友病B提供了一简单而有效的途径.

Parkinson's disease(PD)is characterized by a loss of the dopaminergic neurons of the substantia nigra.The rat with a unilatecal 6-OHDA-induced lesion of the dopaminergic system provided an animal model for PD. The quantifiable rotation of those rats after apomorphine administration and their striatal levels of dopamine(DA)and its major metabolities were used for exploring the degree of DA loss or replacement.The present study was intracerebral grafting(ICG)of the genetically modified cells which could...

Parkinson's disease(PD)is characterized by a loss of the dopaminergic neurons of the substantia nigra.The rat with a unilatecal 6-OHDA-induced lesion of the dopaminergic system provided an animal model for PD. The quantifiable rotation of those rats after apomorphine administration and their striatal levels of dopamine(DA)and its major metabolities were used for exploring the degree of DA loss or replacement.The present study was intracerebral grafting(ICG)of the genetically modified cells which could express tyrosine hydroxylase(TH),in order to induce significant improvement of motor abnormalities and striatal DA levels in rat model.The cultuted primary muscle cells were selected for genetic modification and ICG.The recombinant vectors, pCMVTH(6.04 kb)containing TH gene, were transferred into the myoblasts and myotubes in vitro by using lipofection technique.The genetically altered cells expressing TH in vitro were then grafted into the striatum. The results showed those TH-expressing muscle cells had a long-term survival in vivo and induced a marked decrease in abnormal locomotion and increase in striatal DA levels for rat model.

巴金森氏病以黑质多巴胺能神经元变性减少为特征,以6-羟多巴胺单侧损毁大鼠脑内多巴胺能系统可建立巴金森氏病的动物模型,当给予阿普吗啡后通过检测这些大鼠的旋转圈数和纹状体多巴胺及其主要代谢产物的水平则可反映多巴胺的减少或恢复程度。为达到基因治疗巴金森氏病大鼠模型的目的,本研究将带有酪氨酸羟化酶基因的重组载体-质粒pCMVTH(6.04kb)在体外通过脂质体转染技术转入原代培养的骨骼肌细胞内;再将这些经遗传改造、能表达酪氨酸羟化酶的骨骼肌细胞植入模型大鼠脑的纹状体内。结果显示,这些表达酪氨酸羟化酶的肌细胞可在大鼠模型脑内长期存活,这些模型大鼠的异常运动显现实质性改善,它们的纹状体多巴胺水平明显升高。

 
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