The spirulina protein was completely degraded to the (NH_4)_2SO_4 according to the Kjeldahel procedure. Then,the concentration of (NH_4)_2SO_4 in the digest juice was measured by glutamate dehydrogenase with automatic analyzer,and the spirulina protein concentration was calculated.
Taking the Periodical Work Digest(1989-2002)and Tsinghua Tongfang Full Text Database as examples,the paper has statistically analyzed the progress of periodical resources development and utilization in China.
When using IEEE 802.1X, we must choose an EAP(Extensible Authentication Protocol) type,Such as EAP-MD5(Message Digest 5), EAP-TLS(Transport Layer Security) ,EAP-TTLS(Tunnelled transport Layer Security), etc.
EAP-MD5(Message Digest 5,信息摘要5)、EAP-TLS(Tranport Layer Security,传输层安全协议)和EAP-TTLS(Tunnelled Transport Layer Security,基于隧道的传输层安全协议)等等协议均支持IEEE802.1X。
using 0.125% trypsin+0.02EDTA to digest. The method used mouse ES culture medium composed of DMEM (high glucose) containing 15% FBS, 0.1 mM ? -mercaptoethanol, 0.01 mM non-essential amino acid, 10 ng/ml LIF, 100 IU/ml penicillin, and 100 IU/ml streptomycin.