Using the straight cross experiment design of L25(55), the factors affecting the embryo rescue of crossed progeny between diploid and tetraploid grape cultivars embryo rescue, such as the kinds of culture medium, inoculated time, and plant growth substance concentration were studied.
35~60 days after florescence,embryos of Sultanina(v.vinifera cv.Sultanina) grape were cultured on MS medium supplemented with different concentrations of GA3,IAA and 6-BA in order to study the mechanism of embryo rescue.
To solve the question of incomplete development of embryo in early-ripening grape breeding, orthogonal experimental design of L25( 55 )was adopted to study the embryo rescue technology in early- ripening grape with the crossing embryos of 5 crossing combinations as materials.
In order to explore the most suitable method for rescuing wheat × Leymus angustus immature embryos, two ways of young embryo rescue,embryo culture and embryo-callus culture, was compared on their effects.
The 1 848 florets of Oryza sativa were pollinated with Oryza australiensis and 48 immature seeds were obtained were cultured for embryo rescue. 11 of the 45 seeds could germinate to be F1 hybrid plants. They were all male sterile,the rate of crossability was 0.60%and the rate of germination was 31.1%.
The 1,848 florets of Oryza sativa were pollinated with Oryza australiensis and 48 immature seeds were obtained were cultured for embryo rescue. 11 of the 45 seeds could germinate to be F1 hybrid plants. They were all male sterile, the rate of crossability was 0.60% and the rate of germination was 31.1%.
The ratio of fruit with embryo was 60%-67% in the crosses between C. ×hytivus and cucumber cultigens. Each fruit had about 10-20 embryos, which could survive and develop into plantlets through embryo rescue.
Agar-solidified nutrient media modified from that developed previously for tissue and cell cultures of red clover (2 n = 14) were used for embryo rescue.
All wheat-Agropyron hybrids were obtained by embryo rescue technique.
corymbosum zygotes using embryo rescue techniques, even when embryos were cultured at proembryonic stages.
New interspecific hybrids between alfalfa (Medicago sativa L.) and several perennial Medicago species were obtained by embryo rescue techniques.
rupestris F1 and first backcross generation embryos demonstrated the optimum concentration of 12.5 mM NH4+ for successful embryo rescue; ammonium salt formulation (whether chloride, nitrate or sulfate) was not critical.