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detection     
相关语句
  检测
    Detection of Virulence Related Proteins MRP and EF of Streptococcus suis type 2 and their Cloning and Expression of Genome Fragments
    猪链球菌2型毒力相关蛋白的检测及其基因片段的克隆与表达
短句来源
    Studies on the Detection and Evaluation Method of Genetic Diversity in Characteristic Indigenous Pig Breeds by Microsatellite DNA Markers
    地方猪遗传多样性的微卫星DNA标记检测与评估方法研究
短句来源
    Expression of Nucleocapsid Protein Gene of Porcine Reproductive and Respiratory Syndrome Virus in Soluble Form and Development of an Indirect ELISA for the Detection of Viral Antibodies with the Expression Product
    猪繁殖与呼吸综合征病毒核衣壳蛋白基因的高效可溶性表达和检测血清抗体的间接ELISA方法的建立
短句来源
    Study on Transgenic Detection with PCR and Feeding Safety of Glyphosate-tolerant Soybeans
    抗草甘膦大豆转基因PCR检测及其饲用安全研究
短句来源
    Newcastle Disease Detection by Gene Chip and Typing of Newcastle Disease Virus by RT-PCR Test Coupled with Restriction Endonuclease Analysis
    基因芯片检测新城疫病毒和PCR结合内切酶分析鉴别新城疫强弱毒株的研究
短句来源
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  测定
    Detection and Analysis of the Polymerized Human Serum Albu- min Receptor in Pig Sera
    猪血清中人聚合白蛋白受体的测定与分析
短句来源
    ELISA detection of Rabies antiserum
    狂犬病抗血清ELISA测定的研究
短句来源
    A Method of Detection of Ochratoxin A in Mixed Feedstuffs
    饲料中棕曲霉毒素A的测定方法
短句来源
    Competitive enzyme-linked immunosorbent assay for the detection of T-2 toxin
    霉菌毒素T-2Toxin酶联免疫吸附测定方法
短句来源
    Detection of Kalium concentration from yellow cattle in Huangyuan county
    湟源县黄牛血钾浓度的测定
短句来源
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  诊断
    RAPIDLY DETECTION OF AVIAN EGG DROP SYNDROME (EDS-76) BY USING A PLATE HEMOAGGLUTINATION INHIBITION TEST
    用全血平板凝集抑制试验快速诊断鸡减蛋综合症(EDS_(76))
短句来源
    PRELIMARY STUDIES ON THE METHODS OF RAPID SEROLOGICAL DETECTION AND DIAGNOSIS OF THE BLO ASSOCIATED WITH CITRUS SHOOT-YELLOWING
    柑桔黄龙病的血清学检测与诊断方法的初步研究
短句来源
    A one step RTPCR was developed to detect H5 subtypes of avian influenza virus(AIV). A pair of specific oligonucleotide primers was designed for the detection of AIV H5 subtypes according to 221 hemagglutinin(HA)gene sequences.
    针对家禽中流行较为广泛、危害相对大的H5亚型禽流感病毒的血凝素(HA)基因,通过分析流感数据库221个HA序列,在保守区内用Oligo6.0软件设计并合成了一对引物,建立了用于快速诊断H5亚型禽流感病毒的一步法RT-PCR方法,其扩增的目的片段大小为372bp。
短句来源
    By specificity and sensitity test,the detection method was highly specmc and sensitive. The molecular diagnose method was preliminarily established to detect H5 and H9 subype of avian influenza virus rapidly,sensitively and specifically.
    该检测方法敏感性高,特异性强,初步建立起快速,敏感特异地鉴别检测H5、H9亚型禽流感病毒的分子诊断方法。
短句来源
    Establishment an immunoperoxidase staining technique for the detection of Duck Hepatitis Virus (DHV), to provide the effective examination method for the DHV experimentally diagnosis and the DHV antigen subcellular localization in infected ducklings, then the DHV reproduction spot in the infected cell expounded.
    建立检测鸭肝炎病毒(Duck Hepatitis Virus,DHV)的免疫组化方法,为DHV感染的实验室诊断、感染雏鸭体内的DHV抗原亚细胞定位研究提供有效的检测手段,阐明DHV在感染细胞内繁殖部位。
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  检测方法
    Study on Molecular Detection and Some Resistant Gene Tags in Escherichia Coli O157:H7
    大肠杆菌O157:H7分子检测方法及部分耐药基因标识研究
短句来源
    Studies on Some Biological Characteristics of Duck Enteritis Virus CH Virulent Isolate and Establishment & Application of Real-time PCR Method for Its Detection
    鸭病毒性肠炎病毒CH强毒株部分生物学特性的研究及荧光实时定量PCR检测方法的建立和应用
短句来源
    Study on Gene Engineering Vaccine on Avian Influenza and Development of Latex Agglutination Test to Detection of Its Antibody
    禽流感基因工程疫苗的研究及其乳胶凝集抗体检测方法的建立
短句来源
    ESTABLISHMENT AND COMPARISON OF METHODS USED IN THE DETECTION OF AVIAN LEUKOSIS
    禽白血病检测方法的建立和比较
短句来源
    A Research on Rapid Detection of EDS-76 Virus
    对EDS-76病毒快速检测方法的研究
短句来源
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      detection
    In this article, we focus on a quantum detection problem, where the goal is to construct a tight frame that minimizes an error term, which in quantum physics has the interpretation of the probability of a detection error.
          
    Beyond the applications of quantum detection in quantum mechanics, solutions to this frame optimization problem can be viewed as a generalization of classical matched filtering solutions.
          
    As such, the methods we develop are a generalization of fundamental detection techniques in radar.
          
    Discovery of a New Family of Membrane Progesterone Receptors in Vertebrates and Detection of the Alpha and Beta Subtypes in Mous
          
    This article discusses the problem of the detection of influential cases in nonlinear reproductive dispersion models (NRDM).
          
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    Summary In order to save time in preparing the poorly yielded antigen from the fastidious growth culture of Mycoplasma hyopneumoniae, we directly used the pure culture as an antigen instead. To overcome the slow reactiveness of the mycoplasma-infected pig's sera in the ordinary agglunation test, we incubated the mixture of the tested serum and the culture for 24-48 hours, and examined the stained smears of the mixture under microscope. Two procedures were adopted: (1)0.4 ml of fresh pure luxuriant culture of...

    Summary In order to save time in preparing the poorly yielded antigen from the fastidious growth culture of Mycoplasma hyopneumoniae, we directly used the pure culture as an antigen instead. To overcome the slow reactiveness of the mycoplasma-infected pig's sera in the ordinary agglunation test, we incubated the mixture of the tested serum and the culture for 24-48 hours, and examined the stained smears of the mixture under microscope. Two procedures were adopted: (1)0.4 ml of fresh pure luxuriant culture of Mycoplasma hyopneumoniae was added to a small sterile vial containing 0.1 ml of tested serum inactivated by 56℃ for 30 minutes, mixed well, and incubated in 37℃. The vial was stoppered with rubber cap to prevent evaporation. After 24-48 hourst' incubation, smears were made and stained with Wright's stain for microscopic examination. (2) 0.36 ml of modified Switzer's medium (KM~2), 0.04 ml of fresh pure luxuriant culture and 0.1 ml of tested serum were mixed well in a sterile small vial, and incubated in 37℃ 24-48 hours. Made smears and examined as in (1). The positive reactions appeared earlier in (1) than in (2), while the mycoplasmal cells in the agglutinated clumps were clearer in (2)than in (1). All the serum samples from 54 experimentally infected pigs showed positive reactions in the microagglutination test. A great majority of the positive reactions appeared 8 days after the occurrences of the shadows of lung lesions detected by X-ray. A month later the intensities of reactions reached a peak, maintained for about 6 weeks, and declined thereafter. 5 months Iater positive reactions were still detectable, even when the lung lesions were no longer detectable by X-ray examination. Out of 100 serum samples collected from pigs with lung lesions detected by X-ray in field infected herds,82(82%) showed positive reactions in the microagglutimation test, and out of 16 serum samples collected from pigs without lung lesions by X-ray detection 12 (75%) were negative in the microagglutination test. Antisera against the Annin (a county name in Gansu province) strain of M.hyopneumoniae showed positive reactions with 20 strains of Mycoplasmas colleted from laboratories in differant provinces of China, but did not react with M. hyorhinis, M.synouiae and Acholeplasma laidlawii. The J strain of M. hyopneumoniae from U.S.A. also reacted positively with hyperimmune antiserum against the Annin strain of M. hyopneumoniae.

    显微镜下观察猪肺炎支原体纯培养物与56℃灭活30分钟的待检血清混合,经在37℃培养24—48小时,涂片,以瑞氏染色后镜检。试验的方法有二:〈1〉无菌小试管中装0.4毫升纯培养旺盛的猪肺炎支原体,加0.1毫升灭活待检血清,加橡皮塞,混合培养。〈2〉适宜猪肺炎支原体生长的液体培养基0.36毫升,种入生长旺盛的、纯粹的猪肺炎支原体新鲜培养物0.04毫升,加灭活待检血清0.1毫升,加橡皮塞,混合培养。第〈1〉法凝集较快些,但凝集粒内菌体清晰度不如第〈2〉法。第〈2〉法凝集慢些,但菌体多系新增殖的,故在凝集粒内较为清晰。人工感染54只猪,在经X射线透视发现病变阴影后8天,可出现血清凝集阳性。一个月后达到高峰,並维持六周左右,其后逐渐下降,直至五个月,纵然病变完全消失(X射线透视阴性),仍有可见的阳性凝集反应。经采集野外感染猪群的猪血清共116份,X射线透视阳性者100份,作微粒凝集反应出现阳性者82份,符合率为82%。X射线透视阴性者16份,微粒凝集反应阴性者12份,符合率为75%。所以有此出入,盖因血清中凝集素的出现较X射线透视出现病变阴影为慢,又当病变消失,X射线透视转为阴性时,尚有一段时间凝集反应仍为阳性之故。

    The pulp of feathers from 1,933 chickens was collected and used to detect the Marek's disease (MD) antigen by the immunodiffusion (ID) test with MD specific antiserum. The results of the experiments showed that 104 healthy chickens all gave negative reactions; of the 475 chickens infected experimentally with Marek's disease virus (MDV), 469 (98.73%) were positive; the 56 chickens inoculated with the herpesvirus of turkeys (HVT) were all negative; the 107 chickens infected with avian myeloblastosis virus (AMV)...

    The pulp of feathers from 1,933 chickens was collected and used to detect the Marek's disease (MD) antigen by the immunodiffusion (ID) test with MD specific antiserum. The results of the experiments showed that 104 healthy chickens all gave negative reactions; of the 475 chickens infected experimentally with Marek's disease virus (MDV), 469 (98.73%) were positive; the 56 chickens inoculated with the herpesvirus of turkeys (HVT) were all negative; the 107 chickens infected with avian myeloblastosis virus (AMV) were also negative; a total of 180 chickens in which 60 were infected with Newcastle disease virus(NDV), 60 with avian infectious bronchitis virus (IBV) and 60 with laryngotracheitis virus (LTV) all gave negative reactions; and of the 466 chickens from farms with outbreaks of MD, 252 (54.08%) gave positive reactions. MDV antigens could be detected from the feather tips as early as the 13th or 14th day PI, and most of them were detected 20-30 days PI which appeared to be the optimum time for running the ID tests. It has been found, however, that the percentage of MD detection in clinically convalescent chickens was reduced as the time of running the test was delayed. In addition, when the blood from a positively reacted chicken to the ID test was inoculated into the yolk sacs of 5-day-old chick embryos, typical MDV pocks occurred on the chorioallantoic membranes of most of the embryos 14 days PI. It is thus suggested that the blood of chickens reacted positively to the ID test does contain MDV, and the ID test using MD antiserum to detect the MDV antigens in the pulps of feathers is specific and reliable for the diagnosis of MD in chickens.

    使用已知抗马立克氏病病毒(MDV)血清进行免疫扩散试验,共检查了1,933只鸡的羽髓材料,其中未感染MDV的健康鸡104只(全部阴性),实验感染MDV的鸡475只(469只阳性,占98.73%),火鸡疱疹病毒(HVT)免疫的鸡56只(全部阴性),实验感染鸡成髓细胞增生性白血病病毒(AMV)的鸡107只(全部阴性),实验感染鸡新城疫(ND)、传染性支气管炎(IB)和传染性喉气管炎(ILT)病毒的鸡各60只(全部阴性),非MD发病鸡场的鸡545只(19只阳性,占3.48%),MD发病鸡场的鸡466只(252只阳性,占54.08%)。在实验感染鸡中,MDV羽髓抗原的最早检出时间大约在感染后的第13~14天,最适检出时间大约在20~30天。临床耐过鸡的羽髓抗原检出率随时间的延长而降低。另外,把在非MD发病鸡场检出的羽髓抗原阳性鸡的抗凝全血接种于5日龄的鸡胚卵黄囊,接种后14天检查绒毛尿囊膜,在大多数接种胚中可见典型的病毒痘斑,说明羽髓抗原阳性鸡的血流中确实含有MDV;也就是说,用已知MDV阳性血清检查羽髓抗原来诊断MD的免疫扩散试验是特异而可靠的。

    Throughout this study the same strain of Duck Hepatitis Virus (DHV)was used to prepare the antigen for the detection with the te- chnique of immuoelectroosmophoresis of the positive rate of the anti- DHV substance in the albumin of both the vaccinated breeders and the non-vaccinated breeders respectively.It was found that the positive ra- te in the albumin of the former was significantly higher than that of the latter.The question of the appearance of“positive responses”in the albumin of nonvaccinated...

    Throughout this study the same strain of Duck Hepatitis Virus (DHV)was used to prepare the antigen for the detection with the te- chnique of immuoelectroosmophoresis of the positive rate of the anti- DHV substance in the albumin of both the vaccinated breeders and the non-vaccinated breeders respectively.It was found that the positive ra- te in the albumin of the former was significantly higher than that of the latter.The question of the appearance of“positive responses”in the albumin of nonvaccinated breeders is slso discussed.

    本试验取同一份小鸭病毒性肝炎病毒(DHV)强毒作抗原,通过对流免疫电泳检测免疫母鸭和非免疫母鸭卵清的抗 DHV“阳性率”,发现前者的“阳性率”显著高于后者。对非免疫母鸭卵清出现“阳性反应”的问题进行了讨论。

     
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