The conditions of LSF was optimized as follows: glucose 5%, potatoes 15%, NaNO_3 0.2%, MgSO_4 0.1%, ZnSO_4 0.1%, pH6.0, inoculum 6.5%, and 40 pieces nata (1×1×1cm) in the fermentation liquid, fermented for 13 days at 26℃.
The optimal shaking flask fermentation conditions were: initial pH 5.5,inoculum 15%(v/v),volume charge 135 ml in 250 ml flasks,and nitrogen source 10 ml mixed with 10 g/L yeast extract and 20 g/L tryptone.
Fermentation broth and cell extracts were tested against typed test organisms.
A cellular automata model for simulating fed-batch penicillin fermentation process
The transition rules of CAPFM are designed based on mechanical and structural kinetic models of penicillin batch-fed fermentation processes.
The simulation experimental results show that CAPFM replicates the evolutionary behavior of penicillin batch-fed fermentation processes described by the structured penicillin production kinetic model accordingly.
The forest litter layer can be divided into fresh litter layer (L), fermentation layer (F) and humus layer (H), which may represent different litter decomposition stages.