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   virus isolation 的翻译结果: 查询用时:0.218秒
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virus isolation     
相关语句
  病毒分离
     The multiplex RT-PCR and the conventional virus isolation agreed on the H5N1, H9N2 subtypes determination of 80 swab samples.
     采用多重RT-PCR检测80份棉拭子样品,并与病毒分离鉴定方法比较,二者H5N1、H9N2亚型的鉴定结果完全吻合。
短句来源
     7 days after challenging, among the 9 monkeys, the positive rate was 7/9 assessed by RNA-PCR and 100% (9/9) by DNA-PCR. At the same time the positive rate was only 5/9 assayed by virus isolation from the plasma.
     9只实验猴感染SIV后7天,RNA-PCR结果为7/9阳性,DNA-PCR结果为100%阳性,而血浆病毒分离只有5/9阳性;
短句来源
     The influenza virus isolation and rapid EIA test were both positive in 75 cases of the 111 patients (67.5%).
     111例流感病毒检测阳性患儿中病毒分离与快速诊断二项同时阳性75例,二者阳性符合率67·5%。
短句来源
     The positive rate of virus isolation of the fetal cerebral cortex at day 12,15,19 of gestation was 10%, 40%, 67%. The positive rate in situ hybridization using MIE and LA probes were 30%, 40%, 83% and 0%, 40%,70% respectively.
     3个时间点实验组病毒分离率分别为 10 %、40 %及 6 7% ,MIE和LA寡核苷酸探针原位杂交阳性率为 30 %、6 0 %、83%和 0 %、40 %、70 %。
短句来源
     Two methods for detection of HHV-6 were compared,the results showed: the positiverate was 88.8% by PCR and 38.9%by virus isolation.
     PCR法与病毒分离法相比较,前者HHV-6检出率为88.8%(16/18).后者为38.9%(7/18)。
短句来源
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  病毒分离培养
     Of the 108 nasopharyngeal secrete specimens,21(19.4%),14(13%) and 12(11%) were judged as RSV positive by rapid cell culture,direct smearing and virus isolation respectively.
     108份鼻咽分泌物中,快速细胞培养法检出21份阳性,阳性率19.4%。 直接涂片法检测出14份阳性,阳性率13%,病毒分离培养法检出阳性12份,阳性率11%。
短句来源
     HCMV IgM? HCMV DNA in thesupernatants of amniotic fluid and HCMV DNA in its deposit were carried out by virus isolation and cultivation, ELISA and PCR respectively in 93 pregnant women with primary or secondary active infection of HCMV.ResultsFifty two cases of HCMV(55.8%),11 cases of HCMV IgM(11.6%) and 78 cases of HCMV DNA(83.9%) were confirmed from 93 supernatant samples of amniotic fluid.
     方法应用病毒分离培养、ELISA及PCR技术检测原发感染或继发活动性感染孕妇羊水中的HCMV、HCMV IgM、HCMV DNA以及羊水沉渣中的HCMV DNA。 结果93例羊水上清液标本分离培养出HCMV 5 2例 ,HCMV IgM 11例 ,HCMV DNA阳性 78例 ,阳性率分别为 5 5 .8%、11.6 %和 83.9%。
短句来源
     The detection rate of the clinical application is 94.7%. It is obvious high to 36.8% detection rate of virus isolation and culture;
     临床应用检出率为94.7%,显著高于病毒分离培养36.8%的检出率;
短句来源
     The positive predictive values (PPV) of the PCR and virus isolation assessments performed in all 50 pregnancies (51 gestational sacs) were 92% and 93.7%,respectively.
     聚合酶链反应和病毒分离培养法对50例孕妇(怀51胎)进行评价的阳性预测值分别为92.0%和93.7%。
短句来源
     Clinical specimens of throat washings were obtained from three patients with severe acute respiratory syndrome(SARS) in Zhejiang province and treated with Vero, RD, VeroE6 and Hep-2 cell lines for virus isolation. The cytopathic effect (CPE) was detected in Vero and RD cell lines after clinical specimens had been inoculated for three days.
     从浙江省3例SARS患者中收集含漱液标本,经处理后接种Vero、RD、VeroE6和Hep-2细胞进行病毒分离,培养3d后在Vero和RD细胞中可观察到细胞病变。
短句来源
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  分离病毒
     The study on the brain of suckling mice infected with HFRSV aerosol were made by virus isolation in Veto-E6 cell,antigen detection by BA assay, and virus proliferation observation by electron microscope.
     用Vero-E6细胞分离病毒法、免疫组化法(BA)和电镜观察对HFRS病毒气溶胶感染的乳小鼠的脑进行病毒分离、抗原检测、定位和病毒在脑细胞中增殖的研究,同时进行病理观察。
短句来源
     First, both semi-nested PCR and virus isolation with C6/36 cell were used for assay the DEN-2 in eggs.
     首先利用半套式PCR、C6/36细胞培养分离病毒等方法检测和分离卵内病毒;
短句来源
     Methods Nest ploymerase chain reaction (Nest PCR) was employed to detect viruses in batches from the first generation eggs to the fourth, combined with virus isolation in cultured C6/36 cells and virus titer monitoring by 50 %tissue culture infection dose(TCID50).
     方法采用套式PCR分批检测感染雌蚊的子一代至子四代卵内登革病毒结合C6/36细胞培养分离病毒及TCID50法滴定病毒滴度。
短句来源
     And the results indicated that FA and RPHA are more sensitive; their concordance rates with virus isolation ( VI)were 88.2% and 76.5%, respectively.
     FA和RPHA特异性强,与分离病毒的符合率分别为88.2%和76.5%。
短句来源
     18 positive virus strains were isolated from 66 CSF specimens(isolation rate was 27.3%) and all 4 fecal samples were positive on virus isolation as well.
     从66份患者脑脊液中分离到18株阳性病毒,分离率为27.3%; 4份患者粪便标本分离病毒全部阳性。
短句来源
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  病毒分离的
     The results suggest that PCR is a simple method instead of virus isolation for the determination of HTV -1 infection.
     因而PCR是一种可以取代病毒分离的常规测定HIV-1感染的简便方法。
短句来源
     To elucidate a new technique for virus isolation, the authors conducted a comparative study on 325 pharyngeal secretions using the microplate method and the tube method.
     为了探索病毒分离新技术,采用微量细胞培养法与试管法,对325例肺炎患儿的咽分泌物进行了病毒分离的对比研究。 结果微量细胞培养法共分离出121株病毒,分离率37.2%;
短句来源
     The positive rate of virus isolation within 3 days of disease onset was between 73.58% and 82.05%,but the virus could still be isolated on the 8th day of disease onset.
     患者发病3天内病毒分离的阳性率在73.58%~82.05%之间,发病第8天仍可分离到病毒。
短句来源
     Methods The Results of IgM antibody of serum and measles measles virus isolation were combined with epidemiology investigation data and test result of veccine to analyzw.
     方法 对麻疹疑似病例的血清IgM抗体检测和麻疹病毒分离的检验结果 ,结合流行病学调查资料与疫苗检测结果进行分析。
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      virus isolation
    The diagnosis of mumps meningoencephalitis could be confirmed by serologic methods and by virus isolation.
          
    Correlation of cytomegalovirus (CMV) detection in urine by tissue culture virus isolation, early antigen immunofluorescence test
          
    HCMV infection was detected in the amniotic fluid by polymerase chain reaction, shell vial culture (immunoperoxidase assay) and conventional virus isolation.
          
    Laboratory diagnosis of CRS could only clearly be achieved by virus isolation after the second month of life despite typical clinical features of CRS and multiple organ involvement.
          
    In a total of 1,928 urine samples, detection of HCMV-immediate early antigen in a spin amplified microplate culture by a monoclonal antibody and immunoperoxidase staining (EA-assay) was compared with virus isolation in cell culture.
          
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    This study described the results of virus isolation from the fecal specimens of 34 children suffering from myocarditis. We used human embryonic kidney cell culture and Hela cell line for virus isolation. Three strains of coxsachie virus B_3 and one each of Adenovirus 3, Adenovirus 5, ECHO virus and suspectable Herpes virus were isolated. According to the variations in the neutralizing titers of the acute and convalescent sera to the cultured viruses, it was concluded that...

    This study described the results of virus isolation from the fecal specimens of 34 children suffering from myocarditis. We used human embryonic kidney cell culture and Hela cell line for virus isolation. Three strains of coxsachie virus B_3 and one each of Adenovirus 3, Adenovirus 5, ECHO virus and suspectable Herpes virus were isolated. According to the variations in the neutralizing titers of the acute and convalescent sera to the cultured viruses, it was concluded that 3 cases were caused by Coxsachie virus B_3 and 2 cases by Adenovirus 3 and 5.

    本文报道应用人胚肾单层细胞和HeLa细胞从34例心肌炎患儿的粪便标本中分离到柯萨奇B_3型病毒3株,腺病毒3型和5型各1株,埃柯病毒和疑似疱疹病毒各1株。结合患儿双份血清对自身病毒的中和抗体效价的变动,可以确定其中3例患儿的心肌炎系由柯萨奇B_3型病毒引起,另2例心肌炎的病原分别为腺病毒3型和5型。

    A transmissible EHF related agent was discovered in lung tissue of infected Apodemus agrarius in the course of serial passages for EHF virus isolation in Apodemus agrarius from non-endemic area. Antigenic specificity of the isolates was detected by indirect fluorescent antibody technique ( IFAT ):among 101 convalescent sera of EHF patients tested, 96% were positive; 175 sera of normal persons and patients suffering from other diseases were all negative; out of 20 paired sera of EHF patients, 18 showed...

    A transmissible EHF related agent was discovered in lung tissue of infected Apodemus agrarius in the course of serial passages for EHF virus isolation in Apodemus agrarius from non-endemic area. Antigenic specificity of the isolates was detected by indirect fluorescent antibody technique ( IFAT ):among 101 convalescent sera of EHF patients tested, 96% were positive; 175 sera of normal persons and patients suffering from other diseases were all negative; out of 20 paired sera of EHF patients, 18 showed 4 fold or more rise of antibody titers; 12 sera of EHF patients tested with KHF antigen previously were reexamined with EHF antigen actually with the same positive or negative results; tests made with antisera against reo-virus (type 1) and reovirus-like agent (orbivirus?) gave consistently negative results; blind tests of coded sera of EHF patients and normal persons produced similar expected results; obvious blocking effect of KHF antiserum to immuno-fluorescence elicited by EHF patient's serum was demonstrated, but no such effect was found with polyvalent antisera of reovirus types 1-3. The above results demonstrated preliminarily that the isolated EHF related agent was the causative agent of EHF.

    在用非疫区黑线姬鼠分离EHF病毒的传代中,发现感染鼠鼠肺中存在一种可传代的EHF相关因子。用间接免疫荧光法对其特异性作了检查:101份EHF患者血清96%阳性,175份正常人及其他疾病患者血清全部阴性;20例EHF患者双份血清,18例的恢复期有4倍以上抗体滴度升高;12例EHF患者血清对KHF抗原及EHF相关因子的阴、阳性反应基本一致;抗呼肠孤病毒血清和抗类呼肠孤病毒血清等全部阴性;血清编码双盲法检查和预期结果一致;抗KHF血清对EHF患者血清的免疫荧光反应有显著阻断作用,抗呼肠孤病毒Ⅰ~Ⅲ型多价血清无此作用。上述结果可初步确定,此相关因子是流行性出血热病毒。

    The sera were collected from 89 patients of clinically diagnosed Japanes B encephalitis, 19 of them (21%) could not be confirmed by serologic test. Specimens including stool, pharyngeal, swab and CSF from 51 patients were used for virus isolation. Altogether 11 viruses were isolated. Futher identification was done by means of neutralization test with the sera of patients from whom the viruses were isolated. Three of the 9 patients finally confirmed to be infected with ECHO_(17), coxackie B_3...

    The sera were collected from 89 patients of clinically diagnosed Japanes B encephalitis, 19 of them (21%) could not be confirmed by serologic test. Specimens including stool, pharyngeal, swab and CSF from 51 patients were used for virus isolation. Altogether 11 viruses were isolated. Futher identification was done by means of neutralization test with the sera of patients from whom the viruses were isolated. Three of the 9 patients finally confirmed to be infected with ECHO_(17), coxackie B_3 and adenovirus type 7 respectively.

    89例临床诊断为乙脑病人收集的血清标本,经血清学检查19例(21%)可排除乙脑。从51例病人收集粪便、咽拭子和脑脊液作了病毒分离,共分离出11株病毒。将自身分离的病毒与病人血清作中和试验表明,三例病人分别为ECHO_(17),Coxackie B_3和腺病毒7型的新近感染。

     
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