The leaves at physiological age of 20 days on the medium of MS+0.4 mg/L 6-BA+0.01 mg/L IBA reached the maximum inductivity of chimera buds,or 34.8%. And dark culture reduced the inductivity of chimera buds.
Somatic embryos were induced from cotyledon explants cultured on Murashige and Skoog(MS)medium supplemented with 1.0 mg·L-1 2,4-D after two weeks of culture.
Studied on tissue culture and rapid propagation of Ageratum houstonianum Mill. The results showed that the best explant in tissue culture was leaf. The best media for each stages were as follows: (1) Callus inducing medium: MS+6-BA1mg/L+NAA0.5mg/L;
Recently, Japanese researchers have used this new effective mutation breeding technique combining tissue culture techniques with ion beam irradiation to obtain specific flower colors in rose (Yamaguchi et al. 2003) and carnation (Okamura et al. 2003).
近年来,日本使用离子束辐射联合组织培养方法培育出了花色发生变异的玫瑰(Yamaguchi et al.2003)以及花型花色发生变异的康乃馨新品种(Okamura,et al.2003)。
The activity profiles of the intracellular and extracellular crude extracts showed that the antibiotic producing culture produces two or more compounds, one being intracellular (antifungal), other being extracellular (antibacterial).
Using the osteoblast culture technique, we detected cell behaviors on the biomaterial in vitro by SEM, and the cell affinity of the composite was found to be higher than that of the PLA scaffold.
The influence of woodland soil bulk density on the growth and distribution of fine root system of main planting tree species in the Weibei Loess Plateau was investigated by means of pot culture and field survey.