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culture     
相关语句
  培养
    Culture and Transplantation Research on Donor Cells for Neural Transplantation
    神经移植种子细胞的培养和移植研究
短句来源
    The in Vitro Culture of Rat Neural Stem Cells and Its Directed Differentiation into Neurons
    大鼠神经干细胞的体外培养及向神经元的诱导分化
短句来源
    Activation、Isolation、Culture and Identification of Rat Oval Cells and the Role of p28~(GANK) in Oval Cells Activation and Proliferation
    大鼠肝卵圆细胞活化、分离、培养和鉴定及P28~(GANK)在肝卵圆细胞活化和增殖中作用研究
短句来源
    Assisted Oocyte Activation Technology and Isolation,Culture of Embryonic Stem Cells
    卵母细胞激活技术与胚胎干细胞分离、培养
短句来源
    The Long Culture of Human Peripheral Blood Mononuclear Cell and the Establishment of the Cell Model of Hepatitis C Virus
    外周血单个核细胞的长期体外培养及丙型肝炎病毒细胞模型的建立
短句来源
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  体外培养
    The in Vitro Culture of Rat Neural Stem Cells and Its Directed Differentiation into Neurons
    大鼠神经干细胞的体外培养及向神经元的诱导分化
短句来源
    The Long Culture of Human Peripheral Blood Mononuclear Cell and the Establishment of the Cell Model of Hepatitis C Virus
    外周血单个核细胞的长期体外培养及丙型肝炎病毒细胞模型的建立
短句来源
    BIOLOGICAL CHARACTERISTIC OBSERVATION OF EXPLANT CULTURE OF HUMAN EMBRYONIC NASOPHARYNGEAL EPITHELIUM——Ⅴ. THE GROWTH KINETICS
    体外培养人胚鼻咽上皮的生物学特性观察——Ⅴ.生长动力学研究
短句来源
    BIOLOGICAL CHARACTERISTIC OBSERVATION OF EXPLANT CULTURE OF HUMAN EMBRYONIC NASOPHARYNGEAL EPITHELIUM——Ⅵ. HISTOCHEMICAL STUDY
    体外培养人胚鼻咽上皮的生物学特性观察——Ⅵ.组织化学研究
短句来源
    BIOLOGICAL CHARACTERISTIC OBSERVATION OF EXPLANT CULTURE OF HUMAN EMBRYONIC NASOPHARYNGEAL EPITHELIUM Ⅰ THE CULTURE OF HUMAN EMBRYONIC NASOPHARYNGEAL EPITHELIUM IN VITRO
    体外培养人胚鼻咽上皮的生物学特性观察 Ⅰ人胚鼻咽上皮体外培养
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  培养基
    ② The proliferation and differentiation features of NSCs were observed under phase contrast microscope after the GM1 with different concentrations were added into NSCs’ and serum-free DMEM/F12 culture media.
    2在NSCs培养基和DMEM/F12培养基中加入不同浓度的GM1,观察GM1对NSCs增殖和分化的影响。
短句来源
    Results ① Comparing with control, no significant proliferation of NSCs was coming to pass when the GM1 with low concentration was added into the cell culture medium; however, the size of NSCs clones gradually decreased and the cells were dying when the GM1 with high concentration was added into NSCs' culture medium.
    结果1与不含GM1的NSCs培养基相比,在NSCs培养基中加入低浓度的GM1,NSCs的生长无明显改变,随着GM1浓度的增加,NSCs克隆球体积逐渐减小,细胞逐渐死亡;
短句来源
    ② NSCs,cultured in DMEM/F12 culture medium only containing GM1,neither proliferated nor differentiated, but were dying soon.
    2在DMEM/F12培养基中单独加GM1而不加血清,NSCs克隆球均未见继续生长,也未见分化,而是迅速的死亡。
短句来源
    Conclusion GM1 of low concentration (12.5 μg/mL) has no significant influence on proliferation of NSCs, but GM1 of higher concentration can inhibit the NSCs’ proliferation in culture medium.
    结论在NSCs培养基中,低浓度(12.5μg/mL)GM1对NSCs的增殖无明显影响,但较高浓度的GM1对NSCs的增殖有抑制作用; 在无血清的DMEM/F12培养基中,GM1不能诱导NSCs分化。
短句来源
    Methods The MSCs isolated from human bone marrow were induced by two different culture systems: 5-aza and CMs extract medium.
    方法用5-aza、小型猪CMs裂解液二种方法体外诱导人MSCs的分化,单纯培养基(DMEM)培养为对照组,观察细胞形态改变。
短句来源
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  培养的
    Experimental Study on Culture of Bone Marrow Stem Cells Combined with Chitin in Vitro
    骨髓基质干细胞复合几丁质凝胶体外联合培养的实验研究
短句来源
    Influence of Hypercholesterinemic and Hyperlipoproteinemic Serum and Reduced Oxygen Tension on the Morphology and Growth of Vascular Smooth Muscle Cells in Tissue Culture
    高胆固醇及高脂蛋白血清和低氧分压对培养的血管平滑肌细胞的形态及生长情况的影响
短句来源
    Study on Cell Culture of Mycobacterium Leprae:(1)Multiplication of M.Lepaemurium in Mouse Peritoneal Macrophages in Vitro
    麻风杆菌细胞培养的研究 (Ⅰ)鼠麻风杆菌在小鼠腹腔巨噬细胞中的增殖
短句来源
    DETERMINATION OF COLLAGEN AND CHONDROITIN SULFATE BY HUMAN EMBRYOCHONDROCYTES IN CULTURE
    培养的人胚软骨细胞合成胶原硫酸软骨素的测定及其生物学意义
短句来源
    DETERMINATION OF[~(35)S]-SO_4~(2-)UPTAKE IN HUMAN EMBRYO CHONDROCYTES IN CULTURE
    培养的人胚软骨细胞对〔~(35)S〕-SO_4~(2-)摄入率的测定及其生物学意义
短句来源
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      culture
    The activity profiles of the intracellular and extracellular crude extracts showed that the antibiotic producing culture produces two or more compounds, one being intracellular (antifungal), other being extracellular (antibacterial).
          
    Stability analysis of equilibrium for microorganisms in continuous culture
          
    The problem of equilibrium of non-linear dynamic system for microorganism in continuous culture is considered in this paper.
          
    Using the osteoblast culture technique, we detected cell behaviors on the biomaterial in vitro by SEM, and the cell affinity of the composite was found to be higher than that of the PLA scaffold.
          
    The influence of woodland soil bulk density on the growth and distribution of fine root system of main planting tree species in the Weibei Loess Plateau was investigated by means of pot culture and field survey.
          
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    The paper presents in detail the technique of leucocytes culture from human peripheral blood for the study of human chromosomes. The techniques involve both the standard method and the semi-micro method with some modifications which have been developed in this laboratory to suit our purposes. With the aid of these techniques, cytogenetic analyses for 7 different cases belonging to 4 different types of congenital defects were made, and the main results are briefly described as follows:1. Down's syndrome...

    The paper presents in detail the technique of leucocytes culture from human peripheral blood for the study of human chromosomes. The techniques involve both the standard method and the semi-micro method with some modifications which have been developed in this laboratory to suit our purposes. With the aid of these techniques, cytogenetic analyses for 7 different cases belonging to 4 different types of congenital defects were made, and the main results are briefly described as follows:1. Down's syndrome (4 cases). Most of the cells analyzed in each case showed 47 chromosomes, and the extra chromosome was found to be one of the smallest chromosome in the karyotype. This finding is apparently in agreement with trisomic G-type as previously reported in the literatures. It is highly probable that the origin of this aberration may arise from non-disjunction of one pair of the smallest chromosomes occured in o?genesis, since there is an indication in the rise of the frequencies of Down's syndrome with the age of the mother.2. Testicular feminization syndrome (1 case). The case reported here involves the finding of the same syndrome in 3 successive generations in one family. Chromosomal analysis was available from only one patient and the result demonstrated that most cells showed a chromosome number of 46 with the normal male XY complement, which was found to be in conformity with the discovery that the chromatin was negative on nuclear sexing. This finding is in obvious disagreement with XXY constitution as suggested by some authors to account for the clinical manifestations of this syndrome. Together with the reports of some other workers, analysis of the familial history of the case gives further evidence in support of the suggestion by Grumbach and Barr that this syndrome may be inherited as due to either a sex-linked recessive or a sex-limited dominant factor, though the existing genetic evidence does not suffice to distinguish between the two possibilities.3. Anorchism (1 case). Chromosome analysis of the patient in question revealed the diploid chromosome number to be 46, including an X and a Y chromosome. The nuclear chromatin pattern was also demonstrated to be of male type. Since this case was a sporadic one, it could be assumed to have been originated by mutation. 4. Female pseudohermaphroditism (1 case). A preliminary analysis of the patient revealed the possibility of being an XO(45)/XY(46) mosaic. But this can not be ascertained without further verification from the observations on the skin and bone marrow cultures.

    本文详细地介绍了以外周血液培养来研究人类染色体的技术,这些都是我们实验室几年来根据国外文献作了一些修改,以适合于我们的具体条件而拟订的.同时应用这些方法对若干病例作了细胞遗传学的研究,主要结果如下:1.四例先天愚型:在这些病例中,绝大多数中期细胞的染色体数均为47.核型分析表明为G-三体型.一般认为这是由于母亲在卵细胞形成时发生了染色体不分离的结果.2.睾丸女性化:这里报导了一个很大的家系,这一综合症的患者在该家系中已络续出现了三代.其中的一个病人用于白细胞的染色体研究.结果是正常人的双倍体数(2n=46), 具有正常男性的核型,即XY.口腔粘膜及多形核嗜中性球的性染色质扦查为阴性.这种结果显然不符合于这样的假设,即认为该种综合症的性染色体组成是XXY.结合以往一些作者的研究,我们认为该综合症是以伴性隐性或限性的常染色体显性而遗传的.但从现有证据尚不足以区分这两种的可能性.3.无睾畸形:白细胞的双倍体数为2n=46,显示正常的男性核(XY).性染色质为阴性.鉴于该病例为散发性的,我们认为它有可能起因于基因突变.4.女性假两性畸形:由白细胞的初步分析,认为可能是XO(45)/XY(46)嵌合体.但为了肯定其确切...

    本文详细地介绍了以外周血液培养来研究人类染色体的技术,这些都是我们实验室几年来根据国外文献作了一些修改,以适合于我们的具体条件而拟订的.同时应用这些方法对若干病例作了细胞遗传学的研究,主要结果如下:1.四例先天愚型:在这些病例中,绝大多数中期细胞的染色体数均为47.核型分析表明为G-三体型.一般认为这是由于母亲在卵细胞形成时发生了染色体不分离的结果.2.睾丸女性化:这里报导了一个很大的家系,这一综合症的患者在该家系中已络续出现了三代.其中的一个病人用于白细胞的染色体研究.结果是正常人的双倍体数(2n=46), 具有正常男性的核型,即XY.口腔粘膜及多形核嗜中性球的性染色质扦查为阴性.这种结果显然不符合于这样的假设,即认为该种综合症的性染色体组成是XXY.结合以往一些作者的研究,我们认为该综合症是以伴性隐性或限性的常染色体显性而遗传的.但从现有证据尚不足以区分这两种的可能性.3.无睾畸形:白细胞的双倍体数为2n=46,显示正常的男性核(XY).性染色质为阴性.鉴于该病例为散发性的,我们认为它有可能起因于基因突变.4.女性假两性畸形:由白细胞的初步分析,认为可能是XO(45)/XY(46)嵌合体.但为了肯定其确切的核型,还有待于对病者的皮肤和骨髓作进一步的研究.

    One hundred and fifty-five children in Peking with mental retardation were examined karyologically with the aid of peripheral blood culture techniques. A total of 74 patients were found to have abnormal karyotypes. The results of chromosome analysis are as follows:1. Forty patients had the clinical and cytogenetic features of Down's syndrome, of which 36 were trisomic for chromosome 21 [47, XX(or XY), + 21]; 2 were translocation between D group and G group [46, XX, - D, +t(DqGq)]; 2 were translation between...

    One hundred and fifty-five children in Peking with mental retardation were examined karyologically with the aid of peripheral blood culture techniques. A total of 74 patients were found to have abnormal karyotypes. The results of chromosome analysis are as follows:1. Forty patients had the clinical and cytogenetic features of Down's syndrome, of which 36 were trisomic for chromosome 21 [47, XX(or XY), + 21]; 2 were translocation between D group and G group [46, XX, - D, +t(DqGq)]; 2 were translation between G group and G group [46, XX (or XY), - G, +t(GqGq)].2. 2 patients had trisomic 18 [47, XX, +18].3. 7 had large short arm of G group [46, XX (or XY), Gp +].4. 1 had translocation of long arm in E group chromosome [46, XY, Eq+].5. 1 had large short arm of a D group [46, XX, Dp + ].6. 1 had translocation of long arm in a G group [46, XY, Gq+].7. 1 had an extra small chromosome of the G group [47, XY, + Gq - ].8. 21 had large Y chromosome [46, XYq+].9. 81 had normal chromosome complements [46, XX (or XY)].The clinical and cytogenetic characteristics of the patients were discribed, the relationships between chromosomal abnormality and mental retardation were discussed.

    本文应用染色体组型分析法,研究了155例先天性大脑发育不全儿童的染色体,分析结果可以分成如下几种类型。先天愚型:第21染色体三体征[47,XX(或XY),+21]36例,D/G易位[46,XX,-D,+t(DqGq)]2例,G/G易位[46,XX(或XY),-G,+t(GqGq)]2例;第18染色体三体征[47,XX(或XY)+18]2例;E组染色体长臂易位(46,XY,Eq+)1例;G组长臂易位(46,XY,Gq+)1例;一个额外的G组小染色体(47,XY,+Gq-)1例;此外还有大Y染色体(46,XYq+)21例;G组短臂增大[46,XX(或XY),Gp+]7例;D组短臂增大(46,XX,Dp+)1例。有染色体改变者共74例,未见染色体改变者81例。染色体组型分析可以作为大脑发育不全等遗传疾病诊断的工具之一。

    The dose-response curves of haemopoietic stem cells in irradiated LACA micewere studied with the spleen colony method and the in vitro agar culture technique.The D_0 value of mouse pluripotential stem cells(CFU-S)was 103.4 rads and thatof granuloid committed precursor cells(CFU-C)was 173.7 rads.It is apparentthat the characteristics of CFU-S are different from those of CFU-C.Since a general method for the assay of pluripotential haemopoietic stem cellsin various animals other than the mouse is not available...

    The dose-response curves of haemopoietic stem cells in irradiated LACA micewere studied with the spleen colony method and the in vitro agar culture technique.The D_0 value of mouse pluripotential stem cells(CFU-S)was 103.4 rads and thatof granuloid committed precursor cells(CFU-C)was 173.7 rads.It is apparentthat the characteristics of CFU-S are different from those of CFU-C.Since a general method for the assay of pluripotential haemopoietic stem cellsin various animals other than the mouse is not available at present,we used the invitro agar curture technique and compared the radiosensitivity of canine and mousebone marrow CFU-C.It was found that Do values calculated from the survivalcurves of radiation of canine and mouse haemopoietic stem cells were 58.6 and190.2 rads respectively.From the results thus obtained it is possible to explainthe different radioresistance between the dog and the mouse on the basis of haemo-poietic stem cells.In view of the fact that AET is capable of protecting haemopoieticstem cells on the one hand and reducing the mortality of irradiated animals onthe other,it is reasonable to assume that the haemopoietic stem cells may play animportant role in the radiation damage and recovery of the animal.

    本文研究了在体内外照射条件下小鼠骨髓多向性造血干细胞(CFU-S)和粒系定向千细胞(CFU-C)的辐射敏感性。它们的 D_0值分别为103.4拉德和173.7拉德,反映了CFU-S 和 CFU-C 是两类在性质上不全相同的干细胞群。在目前尚未建立起除啮齿类动物以外的多向性造血干细胞测定技术的情况下,我们应用体外琼脂培养技术,比较了在体外照射条件下狗与小鼠骨髓 CFU-C 的辐射敏感性,它们的 D_0值分别为58.6拉德和190.2拉德,反映了两者在辐射敏感性上存在着明显的差异,为阐明不同种属动物的不同辐射耐受性提供了一个根据。联系到 AET 具有降低造血干细胞辐射敏感性以及提高动物的辐射耐受性的事实,进一步论证了在造血型放射病的发生和发展中,造血干细胞的损伤和修复是起着重要作用的。

     
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