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   restriction endonuclease analysis 的翻译结果: 查询用时:0.011秒
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restriction endonuclease analysis     
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  限制性内切酶分析
     Methods:One hundred and twenty-two healthy people belonging to Han nationality groups were selected to scan three mutation points of DPYD (IVS14+1GA,Exon13 A1627G and Exon11 G1156T) by PCR-SSCP-silver staining method. We validated it by using restriction endonuclease analysis and DNA sequencing technique.
     方法:采用PCR-SSCP-银染色法对122例健康汉族人DPYD的突变位点IVS14+1G→A、Exon13的A1627G和Exon11的G1156T进行筛查,并用限制性内切酶分析及DNA测序分析加以证实。
短句来源
     Restriction Endonuclease Analysis of the Pigeon Poxvirus DNA
     鸽痘病毒DNA限制性内切酶分析
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     The RT PCR product of F 48 E 8 strain was cloned into the pUC18, then tested by restriction endonuclease analysis, the result suggested it was the NP gene of NDV F 48 E 8 strain.
     将F48E8株扩增产物克隆入pUC18载体,经限制性内切酶分析证实为NP基因。
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     RESTRICTION ENDONUCLEASE ANALYSIS OF PSEUDORABIES VIRUS DNA
     伪狂犬病毒DNA限制性内切酶分析
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     Methods DNA samples of 11 thalassaemic children with G6PD deficiency were analyzed for the three commonly reported mutations (G1388A,G1376T,A95G) using natural primers or mis- matched primers mediated PCR followed by restriction endonuclease analysis.
     方法采用自然或错配引物介导的聚合酶链反应(PCR)/限制性内切酶分析,检测11例地中海贫血合并G6PD缺乏症患儿G1388A、G1376T和A95G三种G6PD基因突变类型。
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  限制性酶切分析
     Restriction endonuclease analysis of PCR amplified products above-mentioned was performed by digestion with Rsa I and Hha 1. Our results showed that same restriction endonuclease pattern was present in SSU rDNA of L. tropica and the pathogen causing CL in Xinjiang, China.
     结果显示:采用RsaⅠ进行限制性酶切分析,克拉玛依地区2例CL患者皮肤病变组织标本的PCR扩增产物经酶切后,其电泳图形与L.tropica完全相同。 显示克拉玛依地区CL病原体SSUrDNA的PCR扩增产物与L.tropica存在相同的限制性内切酶图谱。
短句来源
     In order to construct a genomic bivalent oral vaccine of Leishmania donovani and Vibrio cholerae, we amplified a 1.3kb DNA fragment from 7 strains of Vibrio cholerae with primers P1 and P2. Restriction endonuclease analysis of PCR amplified products from 9 strains of Vibrio cholerae was performed by digestion with EcoRⅠ.
     为了构建霍乱弧菌和杜氏利什曼原虫双价口服活疫苗候选株 ,作者采用 PCR对霍乱弧菌毒力表达调控基因 tox R进行扩增及克隆 ,并对霍乱弧菌 tox R基因进行限制性酶切分析
短句来源
     The results revealed an EcoRⅠ site in the central part of toxR gene. The entire toxR gene of Vibrio cholerae Non CT strain 7743 was amplified by PCR with primers P1 and P2, digested with endonucleases BamHⅠ and Hind Ⅲ, then was orientationally cloned into plasmid pAT153. The restriction endonuclease analysis demonstrates that the recombinant plasmid ptR4 contains a 1.3kb toxR gene fragment.
     结果显示 :7株霍乱弧菌均扩增出 1 .3kb的 tox R基因片段 ,tox R基因中部含有 Eco R 酶切位点 ,再将 tox R基因克隆在质粒 p AT1 5 3上 ,对所获的重组质粒 pt R4进行限制性酶切分析 ,证实质粒 pt R4插入的 1 .3kb的 DNA片段为 tox R基因片段
短句来源
     This review mainly summarized the molecular biological methods for the identification of P.multocida,such as PCR,colony hybridization and 16S rRNA gene sequencing,and for the character analysis of P.multocida character research,such as restriction endonuclease analysis,ribotyping,pulsed field gel electrophoresis and PCR fingerprinting.
     本文综述了多杀性巴氏杆菌的PCR、菌落杂交和16S rRNA基因测序等鉴定方法以及限制性酶切分析、核糖体分型、脉冲场凝胶电泳、PCR指纹等特性分析方法。
短句来源
  酶切分析
     Cloning and Restriction Endonuclease Analysis of the Nucleocapsid Protein Gene of NDV Strain F 48 E 8
     新城疫病毒F_(48)E_8株核衣壳蛋白基因的克隆及其酶切分析
短句来源
     The PCR amplifying fragment of VP3_coding gene was cloned into pUC18 at the HincⅡ and SacⅠ sites. Restriction endonuclease analysis was used to identify the recombinant plasmid,and the recombinant plasmid MP13 containing 1.6Kb fragment was obtained and sequenced.
     将该PCR扩增片段在HincⅡ和SacⅠ位点克隆进pUC18质粒载体 ,酶切分析筛选到含 1.6kb基因片段的重组质粒MP13,进一步对该片段进行序列测定及用CLONE软件分析该序列。
短句来源
     The amplified RdRp gene was then ligated to T-Vector, and the positive clone(Number 44) containing HCV RdRp gene was identified by rapid screening method, restriction endonuclease analysis and finally sequencing.
     将该基因克隆到T载体中 ,构建了重组质粒 ,通过克隆快速筛选和酶切分析筛选到含RdRp基因的阳性克隆(克隆号为 4 4 ) ,DNA序列分析证实 4 4号阳性克隆RdRp基因序列与cDNA质粒中的相应序列完全一致。
短句来源
     Colt JM109. Screening with blue-white method and identifying by restriction endonuclease analysis and PCR, two recombinant plasmids, pBaxl, which contains a bax cDNA fragment about 0. 4kb, and pBax2, which contains a bax cDNA fragment of 1. 1kb, were obtained respectively.
     用蓝/白法筛选重组菌落,经酶切分析及PCR鉴定,获得了插入片段大小约为0.4kb及1.1kb的BaxcDNA重组质粒PBaxl和pBax2。
短句来源
     Restriction Endonuclease Analysis of mtDNA of Tetrahymena shanghaiensis
     上海四膜虫(Tetrahymena shanghaiensis)线粒体DNA的酶切分析
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  “restriction endonuclease analysis”译为未确定词的双语例句
     Results The restriction endonuclease analysis demonstrated pET15b-PEP-1-SOD1 contained human full-length SOD1 cDNA and PEP-1-encoding fragments. The PEP-1 encoding sequence and SOD1 cDNA of pET15b-PEP-1-SOD1 by sequencing was coincided with the designed PEP-1 encoding sequence and the human SOD1 cDNA of GeneBank "AB087266".
     结果pET15b-PEP-1-SOD1重组质粒经酶切鉴定含有SOD1 cDNA和编码PEP-1的片段,测序分析证实分别与GeneBank“AB087266”登录的人SOD1 cDNA和合成的PEP-1编码序列完全一致。
短句来源
     Methods After SOD1 cDNA is created from the plasmid pBluescript II SK-SOD1 containing full-length human SOD1 cDNA by designing the primer containing special enzyme,and clone SOD1 cDNA and Xho I and BamH I to construct pET15b-SOD1,pET15b-PEP-1-SOD1 was confirmed by restriction endonuclease analysis and sequencing.
     方法通过设计含有特定酶切位点的引物,以含有全长人SOD1 cDNA的质粒(pBluescript II SK-SOD1)为模板,扩增出SOD1 cDNA,将SOD1 cDNA和人工合成的编码PEP-1的双链寡核苷酸克隆至pET15b原核表达载体上,进行酶切鉴定及测序分析。
短句来源
     Methods: A fusion gene containing anti-CD20 scFv,CD8 molecule and CD3ζ chain was constructed and was cloned into pcDNA3.After confirmed by restriction endonuclease analysis,the fusion gene was used to transfect the human peripheral T lymphocytes through electroporation and expression of anti-CD20 scFv-CD8-TCRζ fusion protein was induced.
     方法:构建包含抗CD20scFv、CD8分子和CD3信号转导链ζ的融合基因,将其克隆入载体pcDNA3中,酶切鉴定正确后电转染入人外周血T淋巴细胞,诱导其表达抗CD20 scFv-CD8-TCRζ融合蛋白。
短句来源
     RESULTS:The cloned cDNA was confirmed to be hBMP7 cDNA. Recombinant pAdtrackcmv hBMP7 and pAd hBMP7 were correctly constructed and confirmed by restriction endonuclease analysis. The titer of purified AdBMP7 could reach as high as 5× 1012 vp/mL.
     结果:成功克隆出hBMP7cDNA,酶切鉴定pAdtrackcmv-hBMP7和pAd-hBMP7质粒正确重组,获得纯化后滴度达到5×1012vp/mL的AdBMP7。
短句来源
     RESULTS: Four recombinant plasmids, pTR421-166、 pTR421-179、 pCDNA3.1-166 and pCDNA3.1-179, were constructed successfully and confirmed correct with restriction endonuclease analysis and nucleotide sequencing.
     结果成功构建了pTR421-166、pTR421-179、pCDNA3.1-166和pCDNA3.1-179四种重组质粒,经限制性内切酶双酶切和核苷酸测序鉴定,编码基因正确无误;
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  restriction endonuclease analysis
DNA restriction endonuclease analysis was adapted for typing ofB.
      
Monoclonal antibody subtyping and restriction endonuclease analysis were performed on both environmental and patient isolates.
      
Potable water was identified as the source of the outbreak based on identical patterns on restriction endonuclease analysis.
      
Restriction endonuclease analysis of mitochondrial DNA from human lung adenocarcinoma cell line SPC-A-1
      
Results of restriction endonuclease analysis, PCR detection and DNA sequencing analysis revealed that the cloned DNA sequence was the same as that reported.
      
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The ND1.860 is a new naphthalene degradative plasmid from Pseudomonas ae-ruginosa AS1.860.Its molecular size is 58kb.The restriction fragments of ND1.860 obtained by complete and partial Hind Ⅲ digestions were cloned into pBR322 pla-smid vectors in Escherichia coli.By restriction endonuclease analysis of 17 hybrid plasmids containing HindⅢ fragment (s) ,the restriction map of plasmid ND1.860 was established for the enzymes Hind Ⅲ,EcoRI and XbaI.

萘质粒ND1.860经限制性核酸内切酶HindⅢ完全消化和部分消化所产生的限制片段,分别在大肠杆菌质粒pBR322中克隆。通过对含有ND1.860HindⅢ片段的17个重组质粒进行限制酶分析,建立了ND1.860质粒的HindⅢ、EcoRⅠ和XbaⅠ种内切酶26个切点的酶切图谱。

A temperate bacteriophage, designated as BLLl, was induced from Bacillus licheniformis 2709 following exposure to mitomycin C or UV. The phage as revealed in electron microscope, showed a hexagonal head ( 40 ×40nm ) connected with a flexible long tail (107×5.7nm) . DNA of the phage BLLl was digested with EcoR Ⅰ, Hind Ⅱ arid BamH Ⅰ, and 8, 15 and 9 fragments were appeared on agarose gel electrophoresis respectively. The molecular weight of the phage DMA was ca.23.4kb by restriction endonuclease analysis,...

A temperate bacteriophage, designated as BLLl, was induced from Bacillus licheniformis 2709 following exposure to mitomycin C or UV. The phage as revealed in electron microscope, showed a hexagonal head ( 40 ×40nm ) connected with a flexible long tail (107×5.7nm) . DNA of the phage BLLl was digested with EcoR Ⅰ, Hind Ⅱ arid BamH Ⅰ, and 8, 15 and 9 fragments were appeared on agarose gel electrophoresis respectively. The molecular weight of the phage DMA was ca.23.4kb by restriction endonuclease analysis, and the G + C content of the DMA was 31.2 mol%. Some other characteristics of this phage were investigated.

碱性蛋白酶生产菌——地衣芽孢杆菌经丝裂霉素C或紫外线处理,均可诱导释放噬菌体,电镜观察表明噬菌体头部外廓呈六边形,有不收缩的尾部(头部40nm×40nm,尾部107×5,7nm),噬菌体BLL1 DNA对限制酶Eco R Ⅰ,HindⅢ和Bam H Ⅰ敏感,分别切割成8,15,和9个片段,经电泳法测定。噬菌体DNA分子量约相当于23.4kb,根据解链温度计算出噬菌体的G+C含量约为31.2摩尔%,噬菌体提纯的外壳蛋白经SDS-聚丙烯酰胺凝胶电泳呈现5条主带,其分子量分别约为78000,72000,55000,39000,35000。

Circular and linear plasmid pIDB 103 contained E. coli galk and gpt genes were microinjected into the cytoplasm of fertilized eggs of goldfish. The exogenous DNA sequences seems to be stable in early embryonic stages of the goldfish. At the begining of embryos injected with circular plasmid, the plasmid DNA sequences were detected as circular conformation by southern blot. From gestrula stage, some plasmd DNA sequences were detected as linear conformation with high molecular weight and comigrated with cellular...

Circular and linear plasmid pIDB 103 contained E. coli galk and gpt genes were microinjected into the cytoplasm of fertilized eggs of goldfish. The exogenous DNA sequences seems to be stable in early embryonic stages of the goldfish. At the begining of embryos injected with circular plasmid, the plasmid DNA sequences were detected as circular conformation by southern blot. From gestrula stage, some plasmd DNA sequences were detected as linear conformation with high molecular weight and comigrated with cellular DNA on agarose gel electrophoresis. The circular plasmid were rescued from the injected blastula by retrasformaing E. coli HB 101. Restriction endonuclease analysis of this DNA suggested that the majority of the injected circular DNA were not modified following replication in goldfish embryos. When fertilized eggs of goldfish injected with linear plasmid pIDB 103, the most exogenous DNA sequences were detected as linear conformation with high molecular weight. Some of which seems to be integrated into the chromosome of goldfish.

用显微注射法把含有E.coli galk和gpt基因的环状和线状重组DNApIDB103分别导入金鱼受精卵的细胞质内。这些注射过的卵子一般都能正常发育。从各不同发育时期的胚胎分离DNA与~(32)P标记的pIDB103探针杂交表明,导入的环状外源重组DNA在胚胎发育的早期,绝大部分以各种环状构型存在。从原肠胚晚期开始,它们逐渐形成串联状高分子量DNA。在尾芽期仍能检测到它们的序列。但尚未证明,它们是否与受体的染色体DNA发生整合。我们从囊胚期的胚胎中回收到了能转化大肠杆菌的环状重组DNA,它的酶切图谱和pIDB103极其相似。导入金鱼受精卵内的线状重组质粒pIDB103,除少量DNA与金鱼的染色体DNA可能发生整合外,其余绝大部分也形成高分子量DNA。

 
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