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pregnant rates
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  妊娠率
     In groups with follicular diameter 24~28mm,20~23mm and 29~30mm,the cycle pregnant rates were 65.52%,23.28% and 20.37%,and the spontaneous abortion rates were 6.02%,40.74% and 45.45%,respectively.
     卵泡直径24~28 mm组、20~23 mm组2、9~30 mm组的周期妊娠率分别为65.52%、23.28%、20.37%,自然流产率分别为6.02%、40.74%、45.45%,周期妊娠率前组显著高于后两组(P<0.01);
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     The(cumulative) pregnant rates of TCu380A and MLCu375SL groups at the end of 36 months were 0.94 and 3.24/100 women respectively.
     36个月末时TCu380A组和MLCu375SL组的累积妊娠率分别为0.94和3.24/100妇女。
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     Pregnant rates of recipients and survival rates of embryos in the 3 trials were as follows respeetively 0%(0/8),0%(0//8); 11.1%(1/9),6.2(1/16) and 50%(3/6),33.3%(5/15).
     三批试验的受体妊娠率和胚胎成活率分别如下:0%(0/8)、0%(0/18)、11.1%(1/9)、6.2%(1/16)和50%(3/6)、33.3%(5/15)。
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     The number of offspring were 127, 142, 133 and 95, the abnormal pregnant rates were 0%, 1.41%, 2.65% and 4.21% for above corresponding groups respectively.
     以上各组母鼠生育子胎数分别为127、142、113、95只,异常妊娠率分别为0%、1.41%、2.65%和4.21%;
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     The pregnant rates with respect to frozen embryo were 38.78%, 45.71% and 66.67% for the ++, B+ and BB animals, respectively.
     移植入冷冻胚胎后,++、B+和BB3种基因型群体的妊娠率分别为38.78%、45.71%和66.67%.
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  受孕率
     Results The pregnant rates were 100%, 100%, 83.33% and 66.67% for control group, 0.25 mg/kg group, 0.50 mg/kg group and 1.00 mg/kg group respectively during 1-week conception, 100%, 100%, 83.33% and 75% for above corresponding groups respectively during 3-weeks conception respectively.
     结果对照组、0.25、0.50和1.00mg/kg组合笼1周雌鼠受孕率分别为100%、100%、83.33%和66.67%,合笼3周雌鼠受孕率分别为100%、100%、83.33%和75%;
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     Results:The successful rates of recanalization and pregnant rates of type Ⅰ and type Ⅱ were 100%,37.5% and 26.5%,0% respectively.
     结果:Ⅰ型输卵管阻塞再通成功率100%,Ⅱ型输卵管阻塞手术再通成功率37.5%; 受孕率前者26.5%,后者为0%。
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     Results:The pregnant rates in normal saline group, prednisone group, Tai-bao high dose group and low dose were 38. 89% , 47. 06% , 70. 00% and 75. 00% respectively.
     结果:生理盐水组受孕率38.89%,强的松组47.06%,胎宝高、低剂量组分别为70.00%、75.00%。
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     Of 38 infertile women ,25 cases become pregnant,the pregnant rates were 67.3%;
     术后随访半年, 38例不孕病人中有 25人受孕,受孕率为 67. 3%;
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     The successful catheterization rate was 95.5% for fallopian tubes and the recanal- ization rate proximal allogian tubes was 80%,Normal intrauterine pregnancy was achieved in total 15 women(33.3%)with in two years follow-up 50% and 17.4% pregnant rates were obtained in secondary infertility and primary infertility,respectively.
     近端输卵管阻塞再通率80%。 随访结果:15例患者正常受孕,受孕率33.3%,其中继发不孕症受孕率50%,原发不孕症受孕率17.4%。
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     Results: The successful pregnant rates of group A , B, C and D were 91.25%, 72.52%, 29.85% and 84.62% respectively. There were significant differences between group A and B (P<0.01), A and C(P<0.01), B and C(P<0.01);
     结果:治疗后妊娠成功率A组为91.25%,B组为72.52%,C组为29.85%,D组为84.62%,A组、B组、C组两两比较差异有显著性(P<0.01);
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     The pregnant rates of embryo transplantation were 49 2% (211/429) in cows of Luxi Yellow Cattle and 63 3% (38/60) in heifers of Holstein Friesian.
     经产黄牛移植成功率 4 9 2 % ( 2 11/ 42 9) ; 育成荷斯坦牛移植成功率63 3 % ( 3 8/ 60 )。
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     Dairy cow were inseminated with routine semen and sexing semen separated by flow cytometry in this experiment and the results showed that the pregnant rates of the treatment group (sexing semen) and the control group (routine semen) were 54.81%(74/135) and 60.61% (40/66) respectively,and there was no significant difference between them (P>0.05).
     本试验用经流式细胞分离仪分离的富含X精子的性控精液及常规精液对奶牛进行人工授精。 结果表明:试验组(性控精液)与对照组(常规精液)情期受胎率分别为54.81%(74/135)和60.61%(40/66),两者之间差异不显著(P>0.05)。
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     Results:Clinic pregnant rates were 19/57(33.33%) and 40/111(36.04%), respectively, there was no significant difference between the investigative group and the control group.
     结果 :研究组 5 7个周期中获临床妊娠 19周期 (3 3 3 3 % ) ,对照组 111个周期中获临床妊娠 40周期 (3 6 0 4% ) ,两组比较无显著性差异 ;
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     Methods Levels of sera anticardiolipin antibody immunoglobulin G,M (ACAb IG-G,M) antisperm antibody (AsAb) and antiovarian antibody (AOAb) were determined by enzyme-linked immunosorbent assay (ELISA) in 164 treatment cycles with IVF-ETand the pregnant rates were analyzed.
     方法 采用酶联免疫吸附法 (ELISA)对 2 0 0 1年 9月~ 2 0 0 2年 8月共 1 6 4个IVF -ET周期中女性血清中抗心磷脂抗体免疫球蛋白G ,M(ACAbIg-G ,M)、抗精子抗体 (AsAb)和抗卵巢抗体 (AOAb)进行测定 ,同时观察IVF -ET周期妊娠结局。
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Ligation of vas deferens without section has been studied by researchers with unsatis-factory results.We have employed a method by silk yarn ligation at three places of the vasdeferens without cutting through it,except for crushing at one point for 4327 cases in thelast twelve years.As the follow up of 343 cases showed,the non-pregnant rate was 97.4%,and complications 1.5%.Among them,semen examination was conducted for 207 cases,of which aspermia rate was 95.7%,corresponding to the sterilization...

Ligation of vas deferens without section has been studied by researchers with unsatis-factory results.We have employed a method by silk yarn ligation at three places of the vasdeferens without cutting through it,except for crushing at one point for 4327 cases in thelast twelve years.As the follow up of 343 cases showed,the non-pregnant rate was 97.4%,and complications 1.5%.Among them,semen examination was conducted for 207 cases,of which aspermia rate was 95.7%,corresponding to the sterilization following vasectomy.We used 48 dogs for experimentation.The results showed successful occlusion of thevas deferens in 95.8%.We consider that the occlusive sterilization mechanism of thelumen of the vas deferens is due to hyperplasia of the fibrous tissues at the point of ligation,crushing and mechanical pressure of the silk ligatures.Because the vas deferens was not cut through,the operation was well received.

本文介绍了输精管不切断结扎术。采用丝线结扎输精管的三个部位,中间一处加以压挫后再结扎而不切断。行术4327例,抽样随访了343例,术后未孕率为97.4%,并发症发生率为1.5%。对随访对象中的207例进行了精液检查,结果精子消失率为95.7%。与输精管切断结扎相比,效果相当。此法的绝育机理主要是结扎线和压挫的机械压迫,使该处纤维组织增生,致使管腔闭塞。

Mature Hu-Young ewes were used as donors and recipients.Supe- rovulation was induced with FSH(300u.)and LH(150u.),and the em- bryos were collected surgically from the uterine horns on Day 6-8(on- set of oestrus=Day o)by flushing with Dulbecco's phosphate-buffered saline(PBS)+5% heated calf serum. Only morphologically normal embryos were used for freezing.Befo- re freezing additiou of cryoprotectant(DMSO)was carried out in six steps(0.25M,0.5M,0.75M,1.0M,1.25M and 1.5M for 7-10 min each). After thawing the DMSO...

Mature Hu-Young ewes were used as donors and recipients.Supe- rovulation was induced with FSH(300u.)and LH(150u.),and the em- bryos were collected surgically from the uterine horns on Day 6-8(on- set of oestrus=Day o)by flushing with Dulbecco's phosphate-buffered saline(PBS)+5% heated calf serum. Only morphologically normal embryos were used for freezing.Befo- re freezing additiou of cryoprotectant(DMSO)was carried out in six steps(0.25M,0.5M,0.75M,1.0M,1.25M and 1.5M for 7-10 min each). After thawing the DMSO in PBS was removed also in six steps(1.sM, 1.25M,1.0M,0.75M,0.5M and 0.25M for 10 min in each concentration of PBS),Embryos were deep-frozen in ampoules(volume 1.0ml)contai- ning 0.25—0.3ml of 1.5 M—DMSO in PBS(Whittingham,1971). The experiments on the freezing of embryos,from 24 August to 27 December 1979,were divided into three trials.In trial Ⅰ the embryos were frozen according to the method of S.M.Willadsen(1976)in sheep, and from-120℃ the ampoules were thawed from -80℃ to -5℃ at 12℃/min,and from -5℃ the ampoules were thawed rapidly in hand.In trials Ⅱ and Ⅲ the embryos were frozen and thawed as descri-bed by S.M.Willadsen (1978) in cow,and from -60℃ the ampoules were plunged directly into liquid nitrogen.The ampoules were transfer-red to an ethanol bath at -50℃ and thawed at 4℃/min t0-10℃.F-rin -10℃ the ampoules were reroarmed rapidly in hand.A total of 49 embryos (47 morphologically normal and 2 abnormal) were transferred to 23 recipients.Four ewes were pregnant,One of them casted a dead lamb.Five lambs were born,and three of them were from one recipient ewe.Pregnant rates of recipients and survival rates of embryos in the 3 trials were as follows respeetively 0%(0/8),0%(0//8);11.1%(1/9),6.2(1/16) and 50%(3/6),33.3%(5/15).

以成年寒羊母羊做供、受体。使用 FSH(300单位)和 LH(150单位),进行供体超数排卵,并在发情周期的第6~8天(发情开始当天=0天)用杜氏磷酸盐缓冲液(PBS)+5%失活犊牛血清,冲洗子宫角手术采卵。只有形态正常的胚胎用于冷冻。胚胎冻前分6步(0.25M、0.5M、0.75M、1.0M、1.25M 和1.5M,每种浓度停留7~10分钟)添加抗冻剂(DMSO)。解冻后也分6步(1.5M、1.25M、1.0M、0.75M 和0.25M,每种浓度停留10分钟)除去 PBS 液中的 DMSO。胚胎在盛有0.25~0.3毫升 PBS 液(Whitting-ham,1971)的安瓿(容量1.0毫升)中冷冻,PBS 液含1.5M 的 DMSO。自1979年8月24日至12月27日,胚胎的冷冻和移植试验分三批进行。第一批胚胎是按 Willadsen(1976)冷冻羊胚胎的方法冷冻的,并降温至-120℃时将安瓿移入液氮。安瓶从-80℃--5℃,以12℃/分钟的速度解冻,从-5℃放入手中快速升温解冻。第二和第三批,胚胎是按 Willadsen(1978)所述及的牛胚胎冷冻和解冻方法进行的,并降温至-60℃将安瓿直接投入液...

以成年寒羊母羊做供、受体。使用 FSH(300单位)和 LH(150单位),进行供体超数排卵,并在发情周期的第6~8天(发情开始当天=0天)用杜氏磷酸盐缓冲液(PBS)+5%失活犊牛血清,冲洗子宫角手术采卵。只有形态正常的胚胎用于冷冻。胚胎冻前分6步(0.25M、0.5M、0.75M、1.0M、1.25M 和1.5M,每种浓度停留7~10分钟)添加抗冻剂(DMSO)。解冻后也分6步(1.5M、1.25M、1.0M、0.75M 和0.25M,每种浓度停留10分钟)除去 PBS 液中的 DMSO。胚胎在盛有0.25~0.3毫升 PBS 液(Whitting-ham,1971)的安瓿(容量1.0毫升)中冷冻,PBS 液含1.5M 的 DMSO。自1979年8月24日至12月27日,胚胎的冷冻和移植试验分三批进行。第一批胚胎是按 Willadsen(1976)冷冻羊胚胎的方法冷冻的,并降温至-120℃时将安瓿移入液氮。安瓶从-80℃--5℃,以12℃/分钟的速度解冻,从-5℃放入手中快速升温解冻。第二和第三批,胚胎是按 Willadsen(1978)所述及的牛胚胎冷冻和解冻方法进行的,并降温至-60℃将安瓿直接投入液氮。将安瓶放入-50℃的酒精浴中,以4℃/分钟的速度解冻至-10℃。从-10℃将安瓿放入手中快速解冻。第三批试验的受体根据母羊的健康和繁殖情况进行了挑选。共49个胚胎(47个形态正常的和2个形态异常的)移入23只受体,4只母羊妊娠。其中一只受体早产,羔羊死亡;一只受体母羊产了3只羊羔,共产了五只羊羔。三批试验的受体妊娠率和胚胎成活率分别如下:0%(0/8)、0%(0/18)、11.1%(1/9)、6.2%(1/16)和50%(3/6)、33.3%(5/15)。

We conducted investigations to observe the reproductive freqency of herds of yellow cow in Northern Fujian in recent three years.Results showed that female yellow cows in Northern Fujian started oestrus at the age of 1.5-2 yrs old.The natural fertiled rate was 58%.The limit of potent breeding years lasted as long as 13-15 years.The time of oestrus mostly occurred on the months of June to December.The period of rhythmic.oestrus averaged 20.9±10.2 days.Oestrus duration was 11.99±6.12 hrs.9.32±3.97 hrs.after...

We conducted investigations to observe the reproductive freqency of herds of yellow cow in Northern Fujian in recent three years.Results showed that female yellow cows in Northern Fujian started oestrus at the age of 1.5-2 yrs old.The natural fertiled rate was 58%.The limit of potent breeding years lasted as long as 13-15 years.The time of oestrus mostly occurred on the months of June to December.The period of rhythmic.oestrus averaged 20.9±10.2 days.Oestrus duration was 11.99±6.12 hrs.9.32±3.97 hrs.after oestrus came the time of ovulation.To obtain higher pregnant rate,the optimum time of insemination should be 9-11 hrs after the female willing to accept mounting of the male.Kinds of female cow.together with well development,growth of ova,method and position of insemination,etc all affected fertilization directly.Within the duration of oestrus,one or two times of insemination made no difference in successful fertilization.61% of the embryo attached to the right side of the uterus in the pregnant cows.18.42% pregnant cows had abnormal oestrus at the state pregnancy.The average pregnant period was 270.9+6.6 days.The time for delivery averaged 1.40±0.5 hrs.Almost 5.37% of the mother cows gave birth at day time.The birth male and female calves approached the ratio of 1:1.The mother cow protected,the foetus quite well and as a general the mother cows reserved good foetus protecting ability.

近三年,我们对闽北群牧黄牛的繁殖规律进行了观察研究。结果表明:闽北黄牛初情期为1.5~2岁,自然繁殖率为58%,可繁殖年限为13—15年,发情月份多集中在6—12月份,发情周期平均20.9±10.2天,发情持续时间为11.99±6.12小时,排卵时间在发情停止后9.32±3.97小时,适宜输精时机应掌握在母牛接受爬跨后的9—11小时,受胎率较高。母牛的类别、膘情、卵泡发育的不同阶段,输精的方法和部位对受胎率均有直接的影响,而情期内输精一次和二次对受胎率的影响差异不显著。妊娠母牛有61%的胚胞附植在右侧子宫角。有18.42%怀孕母牛表现不规则的妊娠发情,妊娠期平均为270.9±6.6天。分娩过程持续时间平均为1.40±0.5小时,有53.7%母牛在白天分娩。公母犊性别比例接近1∶1,母牛有良好的保胎和护仔性能。

 
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