Objective To investigate the effect of mutant-type IκBα(IκBαM)on the growth of human glioblastoma multiform(GBM)cell lines and the expression of VEGF and IL-8.Methods Human GBM cell lines were used,transfected with IκBαM gene and the cell growth rate were examined.
Methods The surgically removed brain tissues of patients with IE(n=36)and normal controls(n=8)were collected,and scanned to detect differentially expressed genes by using cDNA chip with 4096 human target genes. Then the can- didate gene was selected,and FQ-PCR with gylceraldehyde-3-phosphate dehydrogenase(GAPDH)as internal con- trol was used to confirm the selected gene.
Background and Objective: Diffusion weight imaging (DWI) was the unique method to present the changes of the micro geometry structure of the human body, the motion of the water molecule and the temperature of the cell by performing the molecular diffusion measurement and imaging.
Prion diseases include a series of diseases such as CJD、GSS、FFI、Kuru of human and Scrapie、BSE、CWD of animals. Prion diseases are characterized by neuronal celldeath, glial proliferation and deposition of prion peptide aggregates.
Objective To construct genetically engineered cells that secrete human TH and GDNF at the same time by stable co-transfection pcDNA3.0/hTH and pcDNA3.1/hGDNF in SH-SY5Y cells and study their effects on the gene therapy of Parkinson′s disease (PD).
Mesenchymal stem cells(MSCs) have been isolated from bone marrow in human and in rodents,which have the capability to differentiate into multiple kinds of cells and can be transplanted into brain via many pathways.
One was PKI (the form the Camp-dependent protein kinase inhibitor) gene. The deduced amino acid sequence of the human PKI P showed 30% identity to human PKI, 23% identity to human PKI and 74% identity to murine PKI.
Using a bacteria expression vector constructed to synthesize the complete sequence of the human PKI has produced human PKI P protein with high purity, which inhibits phosphotransferase activity of C subunit in vitro.
The pharmaceutical sciences are advancing rapidly in the post-genomic era of the 21st century with the completion of the human genome, as well as those of many other organisms including bacteria and parasites, and the rapid advances in proteomics.
Aqueous and organic solvent extracts tested by agar-well diffusion method against 12 human pathogenic bacteria and 3 fungal strains showed activity to most of the organisms.
These findings provide compelling evidence that DNA is the ultimate target of these drugs that act on the human genome.
The pharmacokinetics of α- and β-diastereomers of Arteether, a well-known antimalarial drug and its active metabolite dihydroartemisinin (DHA) were studied in Sprague-Dawley rats, Rhesus monkeys and human volunteers.
STUDIES ON NOVEL NON-IMIDAZOLE HUMAN H4 RECEPTOR ANTAGONISTS USING GFA AND FREE-WILSON ANALYSIS