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teeth germs
相关语句
  牙胚
    In situ hybridization methodology study with teeth germs
    牙胚组织原位杂交方法学探讨
短句来源
    Objective To discuss the best method for in situ hybridization with teeth germs.
    目的探讨牙胚组织原位杂交的最佳方法。
短句来源
    Methods Oligonucleotide probes labeled with digoxigenin were used in in situ hybridization to inspect DSPP mRNA expression in various teeth germs.
    方法用牙本质涎磷蛋白DSPP特异性寡核苷酸探针,检测不同时期牙胚DSPPmRNA的表达。
短句来源
    Conclusion In situ hybridization method established with teeth germs showed sensitivity and specificity.
    结论所用的牙胚组织原位杂交方法敏感性高,特异性高。
短句来源
  牙胚
    In situ hybridization methodology study with teeth germs
    牙胚组织原位杂交方法学探讨
短句来源
    Objective To discuss the best method for in situ hybridization with teeth germs.
    目的探讨牙胚组织原位杂交的最佳方法。
短句来源
    Methods Oligonucleotide probes labeled with digoxigenin were used in in situ hybridization to inspect DSPP mRNA expression in various teeth germs.
    方法用牙本质涎磷蛋白DSPP特异性寡核苷酸探针,检测不同时期牙胚DSPPmRNA的表达。
短句来源
    Conclusion In situ hybridization method established with teeth germs showed sensitivity and specificity.
    结论所用的牙胚组织原位杂交方法敏感性高,特异性高。
短句来源
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Aim: To investigate the effects of bFGF on development and differentiation of tooth germ. Methods: The rat tooth germs were cultured for up to 3, 6 or 9 days respectively with or without exogenous human recombinant bFGF (hbFGF) at concentration of 10 ng/ml. Specimens were processed for histology and sectioned at 6μm. Results: hbFGF showed the precocious tooth development. The control group progressed to differentiation of odontoblasts and little predentine matrix while germs treated with...

Aim: To investigate the effects of bFGF on development and differentiation of tooth germ. Methods: The rat tooth germs were cultured for up to 3, 6 or 9 days respectively with or without exogenous human recombinant bFGF (hbFGF) at concentration of 10 ng/ml. Specimens were processed for histology and sectioned at 6μm. Results: hbFGF showed the precocious tooth development. The control group progressed to differentiation of odontoblasts and little predentine matrix while germs treated with hbFGF demonstrated well-defined odontoblast and predentine matrix at 9 days of culture. Conclusion: bFGF might function to regulate the rates of tooth development during rat molar morphogenesis and dentinogenesis.

目的:观察外源性bFGF对体外培养牙胚发育和分化的作用。方法:采用体外培养17dSD胎鼠第一磨牙牙胚,培养液中加入10ng/ml人重组bFGF(hbFGF),分别培养3、6、9d,组织学观察。结果:外源性hbFGF可使大鼠牙胚的细胞分化和牙本质基质分泌加快。结论:外源性bFGF可以促进大鼠牙胚的发育,加速牙齿发育。

Objective To observe the arrange ment of fetus tooth germ in jaw and the regular pattern of development course of the length of dental arch. Methods The maxillary and mandible of 40 fetuses (different ages) were cut horizontally with toothwithjaw continual tissue slices technique, observation and statistics. Results There was tooth germ crowding after 28 weeks, remarkable in the maxillary. The development of the upper dental arch was earlier, but the down dental arch faster. Conclusion...

Objective To observe the arrange ment of fetus tooth germ in jaw and the regular pattern of development course of the length of dental arch. Methods The maxillary and mandible of 40 fetuses (different ages) were cut horizontally with toothwithjaw continual tissue slices technique, observation and statistics. Results There was tooth germ crowding after 28 weeks, remarkable in the maxillary. The development of the upper dental arch was earlier, but the down dental arch faster. Conclusion The fetus dental arch was crowding noteworthily and the length of the down dental arch has basically completed at birth.

目的观察胎儿期牙胚在颌骨内的排列情况及牙弓长度的发育进展规律。方法应用牙连同颌组织连续切片技术,对40例不同胎龄胎儿的上下颌骨作水平方向切片,观测并统计。结果胎儿28周后牙胚拥挤,上牙弓拥挤更显著;上牙弓发育较早,但下牙弓发育速度快于上牙弓。结论胎儿期牙列拥挤明显,下牙弓长度主要在胎儿期已基本完成。

Aim: To prepare a rabbit anti-rat amelogenin IgG antibody and study it's specificity. Methods:complete/imcomplele Freund's adjuvant were used when immunized the rabbit with amelogenin A 1 subcutaneously; The specific IgG antibody was further purified through DE-32 cellulose.The immunological reaction between this antibody and the enamel matrix was determined by immunoelectrophoresis; The histological specificity of this antiserum was tested by immunohistohistochemistry method both in rat incisor...

Aim: To prepare a rabbit anti-rat amelogenin IgG antibody and study it's specificity. Methods:complete/imcomplele Freund's adjuvant were used when immunized the rabbit with amelogenin A 1 subcutaneously; The specific IgG antibody was further purified through DE-32 cellulose.The immunological reaction between this antibody and the enamel matrix was determined by immunoelectrophoresis; The histological specificity of this antiserum was tested by immunohistohistochemistry method both in rat incisor and human tooth germ. Results: The three groups of amelogenin A 1,A 2,A 3 reacted with the anti-A 1 antibody,but had a different shape of precipitin,and the enamelin E1 had no cross reaction with this antiserum. Anti-cytokeratin monoclonal antibody can only react with ameloblast layer, mean while, the anti-amelogenin serum reacted with ameloblast layer having the most positive area on the Tomes processes and the outer enamel layer. Conclusion: The prepared anti-amelogenin A 1 antiserum has a specific immune reaction with the extracellular matrix of ameloblast——amelogenin.

目的:制备抗大鼠釉原蛋白的抗血清,并研究其在组织学上的特异性。方法:采用弗氏完全/不完全佐剂,经皮下多点注射免疫;将所得抗血清用DE-32纤维素纯化其IgG抗体;用微量免疫电泳法测试抗体与釉质基质蛋白之间的免疫反应;用免疫组织化学法测试抗体与大鼠切牙及人牙胚中的组织特异反应。结果:所制备的抗血清与釉原蛋白三种组份A1、A2、A3之间都发生沉淀反应,但沉淀弧形及位置不同,不与釉蛋白E1发生反应。抗角蛋白单克隆抗体与成釉细胞发生强阳性反应,但不与釉质基质发生反应。抗釉原蛋白抗体与成釉细胞发生阳性反应,但在童氏突与釉质浅层区反应最强,不与牙本质细胞发生交叉反应。结论:抗大鼠釉原蛋白抗体能特异地与成釉细胞外基质———釉原蛋白发生免疫反应。

 
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