Chimerism exists in both the thymus and spleen of the tolerant B6 mice. The percentage of cells of BALB/c origin in the spleen of B6 mice in the tolerant mice was 15.86%,10.57%and 1.77% on day 15,30 and 70 after skin grafting,and in the thymus was 8.19%,5.72% and 1.87%,respectively. The chimerism level gradually declined with time.
Conclusion:Above results indicated that the auto-Kc activated the Th2 cells by indirect recognition pathway,and then the Th2 cells secreted cytokines such as IL-10 and IL-4 which in turn inhibited the proliferation of the Th1 cells,leading to a local immuno-suppression for allo-rejection in the intermingled skin grafting. [
Methods On immunosuppresion with anti CD 3 antibody,bone marrow cell suspension extracted from donor strain C57BL/6 rats was infused into recipient BALB/C rats after skin grafting and the allograft survival time was observed.
In the three groups of SD zinc or SD silver plus skin grafting at 15 d after combined injury and SD zinc alone,the 15-day survival rates were 63%,69% and 75% respectively,and significantly higher than that of control group (42%).
Within other 22 patients(32 hands) treated with palmar aponeurosis and skin resection combined with free skin grafting,20 patients were male and 2 patients were female,with an average age of(53.7±8.9) years ranging from 23 to 64 years.
Result: 9 out of the 32 cases (28. 1% ) of diabetic foot ulcer were successfully repaired by skin regenerative medical technique alone, 12 cases (37. 5% ) were repaired by skin regenerative medical technique associated with skin grafting, 5 cases (15. 6% ) were repaired by skin regenerative medical technique associated with skin flap and 6 cases (18. 8% ) were subjected to amputation.
Methods One hundred and thirty-seven cases of PWS were classified with Waner′s standard, in which 129 cases of type 1 or 3 were treated by Vbeam dye laser and 8 cases of type 4 were accepted skin grafting or expanded skin flaps transferring operation after local lesion resection.
Methods: 20 patients with scar contrcture in the perineal area were treated in our hospital from Jan. 1991 to Jan. 2000. Freee skin grafting + local skin flap transposition and skin flap transposition were used.
The role of the metabolic status of the donor and the recipient in graft survival was studied with skin grafting between genetically different mouse strains (BALB/C, C3HA, DBA/2, and C57Bl/6).
If impossible and there is no need for subsequent abdominal re-exploration, the open wound should be covered with split-thickness skin grafting directly over the bowel loops.
Lack of improvement over a 2-to 4-week period is predictive of eventual lack of response to therapy and suggests the need for adjunctive methods to achieve success, such as oral pentoxifylline, tissue-engineered skin, or skin grafting.
Patients with defects of the dorsum of the hand or cubital fossa area who underwent debridement, temporary wound coverage and skin grafting (n = 11) presented complete healing 52 days after the referral.
The healing time of defects of the forearm (n = 2) and dorsum of the foot (n = 5) was a mean of 15 days after skin grafting.