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dna breakage     
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  dna断裂
     When combined with 0.2 μmol·L -1 ACR, the DNA breakage level reduced to (1.0±0.3) , (3.9±0.4) and (6.1±0.3)μm, respectively.
     上述浓度MIT与 0 .2μmol·L- 1的ACR共同作用后 ,DNA断裂长度依次减低为 (1.0± 0 .3) ,(3.9± 0 .4 ) ,(6 .1± 0 .3) μm。 两者相比差别明显 (P <0 .0 1)。
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     Expression of bcl 2 gene and DNA topoisomerase II activity were examined with Northern blot analysis and plasmid DNA breakage analysis, respectively.
     Northern杂交分析和质粒DNA断裂分析检测bcl 2基因表达和DNA拓扑异构酶II活性。
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     Dying with Hoechst33342 and observing the DNA breakage by fluorescence microscope;
     Hoechst33342荧光染色,观察DNA断裂情况;
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     Conclusion:DNA breakage is the main marker of apoptosis and this can be differentiated from necrosis.
     结论:DNA断裂是细胞凋亡的主要标志,此可区别于坏死细胞。
短句来源
     Conclusion 125IUdR can lead to the inhibition of G1 stage cell multiplication and DNA breakage,inducetumor cell apoptosis by electrolytic dissociation.
     结论125IUdR通过电离作用导致G1期细胞增殖阻滞及DNA断裂、诱导肿瘤细胞凋亡。
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  dna损伤
     But the level of DNA breakage induced by Ni 2O 3+Na 2SeO 3 was lower than Ni 2O 3 -treated group (P<0.01).
     而Ni2O3+Na2SeO3 处理组DNA损伤程度低于Ni2O3 组,差异有显著性(P<0.01)。
短句来源
     Results The level of DNA breakage induced by Ni 2O 3 was higher than the control group(P<0.01).
     结果 Ni2O3 处理HLF细胞4 h,SCGE检测出DNA损伤程度高于对照组,差异有显著性(P<0.01)。
短句来源
     Enriched UO2F2 could also result in DNA breakage in germ cells.
     浓缩铀UO_2F_2可导致雄性生殖细胞DNA损伤,但不同发育阶段的生殖细胞对其敏感性呈现出差异。
短句来源
     SCGE (Single cell gel electrophoresis) was used to detect the DNA breakage in the BALF, lung cells and PBMC.
     同时,PBMC和BALF细胞DNA损伤之间存在正相关。
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     The Effect of Different Ion Radiation on in Vitro Plasmid DNA Breakage and its Transforming Activity
     不同离子辐照对离体质粒DNA损伤与转化活性的影响
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  dna链断裂
     Measurement of DNA Breakage and Breakage Repair In Mice Spleen Cells Induced by Ionizing Radiation
     电离辐射诱发小鼠脾细胞DNA链断裂及修复
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  dna断裂作用
     Study on mechanism of formaldehyde-induced DNA breakage and repair
     甲醛致DNA断裂作用的机制及修复的研究
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     The molecular mechanism for chromosomal aberration and micronuclei induced by holmium ions was most probably DNA breakage to root-tip c ells of vicia faba.
     钬离子诱导蚕豆根尖细胞染色体畸变和微核产生的分子机制主要为DNA断裂作用
短句来源
     The Formaldehyde-induced DNA breakage, its mechanism and repair was studied with single cell gel electrophoresis (SCGE) in the experiment.
     应用单细胞凝胶电泳技术研究了甲醛致DNA断裂作用、作用机制以及断裂的修复.
短句来源
     It concluded that formaldehyde could assuredly induce DNA breakage through ROS, and the breakage could be repaired completely in 90 minutes.
     甲醛引起DNA断裂作用是通过活性氧自由基介导的,且在90min时基本上可被完全修复.
短句来源
     Conclusion Holmium ions of rare earths have genotoxicity to bone-marrow cells of mice,and DNA breakage may be one of its molecular mechanisms.
     结论钬化物对小鼠骨髓细胞具有一定的遗传毒性,DNA断裂作用可能是其分子机制之一。
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  dna breakage
Complete correlation between the three biological activities-cytotoxicity, inhibition of DNA topoisomerase II, and induction of protein-linked DNA breakage-was also not observed.
      
However, compared to the corresponding 4'-0-demethyl analogues, the 3',4'-O,O-didemethyl compounds have a similar potency in inhibition of DNA topoisomerase II but are less active in causing cellular protein-linked DNA breakage.
      
Compounds 5-13 and 15-17 are more potent than etoposide in causing DNA breakage, while compounds 9, 10, 13, 14, 16, and 20 are more active than etoposide in their inhibition of the human DNA topoisomerase II.
      
Synergistic Cytotoxicity, Apoptosis and Protein-linked DNA Breakage by Etoposide and Camptothecin in Human U87 Glioma Cells: Dep
      
- We propose that PARP-associated polymers may recruit signal proteins to sites of DNA breakage and reprogram their functions.
      
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The in vifro double-strand cleayage activity of bleomycin A_5 (China), bleomycin A_2B_2 (Japan), and hleomycin A_2B_2 International Reference Preparation on human gastric carcinoma cellular DNA (from SGC-7901 cell line), its-50kb fraction and human tongue carcinoma cellular DNA (from Tca-8113 cell line) have been studied by the gel electrophoresis analysis. The bleomycin-human DNA interaction models have been shown by the rapid minigel electrophoresis and UV fluorescence photography after ethidium...

The in vifro double-strand cleayage activity of bleomycin A_5 (China), bleomycin A_2B_2 (Japan), and hleomycin A_2B_2 International Reference Preparation on human gastric carcinoma cellular DNA (from SGC-7901 cell line), its-50kb fraction and human tongue carcinoma cellular DNA (from Tca-8113 cell line) have been studied by the gel electrophoresis analysis. The bleomycin-human DNA interaction models have been shown by the rapid minigel electrophoresis and UV fluorescence photography after ethidium bromide dyeing. The effects of incubation period and bleomycin's concentration have been investigated. Human tumour cellular DNA 1mcg, in the presence of dithiothreitol, incubated with~0. 27mM bleomycin at 37℃ for 30 minutes,the double-strand DNA breakage effects were shown on the geJ pattern; after 120 minutes incubation, almost all the DNA molecules were broken. Human tumour cellular DNA in the presence of dithiothreitoi, incubated with series diluted concentrations of bleomycin at 37℃ for 120 minutes, the effects of ouble-strand DNA cleavage were produced with bleomycin concentration as low as~0.67μM. There was no apparent difference identified between bleomycin-human gastric carcinoma cellular DNA and bleomycin-human tongue carcinoma cellular DNA interaction gel patterns.

本文观察比较了博莱霉素A_5(国产)、博莱霉素A_2B_2(日本)和博莱霉素A_2B_2国际标准品对于人体胃癌细胞的总脱氧核糖核酸和其50kb部分,以及人舌癌细胞的总脱氧核糖核酸的体外断链效应。作用后脱氧核糖核酸经琼脂糖微型快速电泳和溴化乙锭染色后,紫外荧光摄影。显示三种博莱霉素产品对人癌细胞双链脱氧核糖核酸的作用模型电泳图。并观察了作用时间及药物浓度的效应。人体DNA10μg在博莱霉素400μg/ml(~0.27mM)浓度下,37℃作用30分钟后,即可观察到人体DNA的断裂效应;37℃作用120分钟后,几乎全部人体DNA分子均被切割断链。人体DNA20μg在系列浓度的博莱霉素存在下,37℃作用120分钟,观察到博莱霉素断链的最低有效浓度为1μg/ml(~0.67μM)左右。没有发现博莱霉素-人胃癌细胞脱氧核糖核酸与博莱霉素-人舌癌细胞脱氧核糖核酸的作用模型之间有何明显差别。 本文还讨论了以人体细胞脱氧核糖核酸为对象,研究抗癌药物作用模式的实践意义,和本实验系统正被应用于人体“癌基因”(Oncogenes)有关研究的价值。

Pingyangmycin was produced by Pingyangnesin sp No. 74 which was isolated from mud at Pingyang, Zhejiang China. The structure and properties of Pingyangmycin are the same as those of bleomycin A-g. Pingyangtnycin-induced cellular DNA breakage was observed in this experiment. DNA breakage in vitro was found by agarose gel electrophoresis and UV fluoresene photography after ethidium bromide staining 0. 5ug cellular DNA incubated with a series of drug concentrations for 2h and with a series of...

Pingyangmycin was produced by Pingyangnesin sp No. 74 which was isolated from mud at Pingyang, Zhejiang China. The structure and properties of Pingyangmycin are the same as those of bleomycin A-g. Pingyangtnycin-induced cellular DNA breakage was observed in this experiment. DNA breakage in vitro was found by agarose gel electrophoresis and UV fluoresene photography after ethidium bromide staining 0. 5ug cellular DNA incubated with a series of drug concentrations for 2h and with a series of drug exposure times at a dose of 200 ug/ml. The mininal concentration and exposure time of DNA breakage is 5 ug/ml and 10 min. DNA molecule was observed under electron microscope with basic protein mounting technique. The natural spiral DNA long strand (X =5.95 um+1.95)was cut down into short DNA fragments ("X =0.75 n.m+0.24)by 10 ug/ml drug for 2h_ The length of untreated DNA is significantly longer than that of the drug treated one(P<0. 01).

用琼脂糖电泳及DNA电镜技术观察平阳霉素对HeLa细胞DNA的体外断裂作用。药物处理的DNA经琼脂糖电泳及溴乙锭染色后,紫外荧光摄影。0.5μg DNA与5μg/ml药物作用2h及200μg/ml作用10min即可见DNA断裂。应用碱性蛋白膜展层法制作DNA电镜标本,5μg/ml DNA与10μg/ml药物作用2h可见自然弯曲的DNA长链(±SD=5.97±1.95μm)被切割成DNA短段(±SD=0.73±0.24μm),对照组DNA长度长于药物处理组(P<0.01)。

Cleavage action of pBR322 DNA by bleomycin(BLM), bleomycin A 5(BLMA 5 ) and peplomycin (PEP) was determined by electrophoretic analysis in agarose gel. Single-and double-strand breaks of DNA could be induced by BLM, BLMA 5 and PEP in the presence of ferrous ion. No specific incision point was observed in the DNA strands. DNA breakage may partially explain the mechanism of anticancer action of these antibiotics-The cleavage extent of DNA was proportional to the concentration of drugs...

Cleavage action of pBR322 DNA by bleomycin(BLM), bleomycin A 5(BLMA 5 ) and peplomycin (PEP) was determined by electrophoretic analysis in agarose gel. Single-and double-strand breaks of DNA could be induced by BLM, BLMA 5 and PEP in the presence of ferrous ion. No specific incision point was observed in the DNA strands. DNA breakage may partially explain the mechanism of anticancer action of these antibiotics-The cleavage extent of DNA was proportional to the concentration of drugs and also to the concentration of the divalent ferrous ion. Magnesium, barium and cobalt ion may also induce DNA breaks without ferrous ion in reaction mixture. The cleavage action of BLM, BLMA 5 and PEP on DNA could be blocked if the abovementioned four kinds of ion had been removed. When the reaction temperature was at 37℃, the cleavage action of three drugs on DNA was rapid but when the temperature decreased to 4℃. the cleavage speed of DNA could be slowed.

本文用琼脂糖凝胶电泳分析测定了博莱霉素(BLM),平阳霉素(BLMA_5)和匹莱霉素(pEP)对pBR322 DNA的断裂作用。在二价铁离子的存在下,BLM、BLMA和FEB能导致DNA单链和双链的断裂。DNA断裂是非特异性的。其断裂程度与药物的浓度成正比,也与二价铁离子的浓度成正比。去除Fe(Ⅱ),而有镁,钡和钻离子存在下,上述药物仍可引起DNA链断裂。去除上述4种离子可以阻止BLM、BLMA_5和pEP对DNA的致断作用。当反应温度为37℃时,上述三种药物对DNA的断裂作用是迅速的。降低反应温度可以减慢DNA的断裂速度。

 
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