To study the regulation of bFGF,ATRA,VD3,DEX and BMP-2 on the differentiation of neural stem cells and the relation between the expression of retinoic acid receptor mRNA and the processes of differentiation.
Objective:To investigate the regulatory effect of nuclear receptors including peroxisome proliferator activated receptorγ (PPARγ) and α(PPARα),retinoic acid receptor(RAR),retinoid X receptor (RXR),vitamin D receptor (VDR),thyroxin receptor (TR) and glucocorticoid receptor (GR) agonist on aromatase activity in human ovarian granulosa and breast cancer MCF 7 cells.
Cells separated from lymph nodes were divided into 5 groups: Retinoic acid (RA), Retinoic acid plus Ro 41-5253, retinoic acid receptor antagonist (RA+Ro), lipopolysaccharide (LPS), lipopolysaccha-ride plus Retinoic acid (LPS+RA) and control cultured in vitro.
Methods Stellate cells were isolated from healthy rat livers and cultured. After stimulated with 1 ng/ml transforming growth factor beta 1, subcultured hepatic stellate cells were treated with or without 1 nmol/ml retinoic acid. MTT assay, immunocytochemistry for p16, p21, p27 and α-smooth muscle actin protein, in situ hybridization for retinoic acid receptor beta 2 and p16, p21 and p27 mRNA and quantitative image analysis (partially) were performed.
Aim: To characterize the changes of retinoic acid receptor β (RARβ) expression in esophageal precancerous and cancerous lesions from the subjects at high and low incidence areas for esophageal cancer in Henan.
The results showed that the expressions of involucrin protein and retinoic acid receptor β inABA group had been higher than that of control group so as to upraise the level of caspase-3 and start up the cell apoptosis.
Our previous data indicated that the combination of ligands for peroxisome proliferator-activated receptor γ (PPARγ) and retinoic acid receptor (RAR) induces apoptosis of breast cancer cells in vitro and in a murine model.
Using monoclonal antibodies for retinoic acid receptor alpha (RARα), we found nuclear staining in melanomas and lung carcinomas metastatic to brain and only rarely in gliomas.
Paucity of Retinoic Acid Receptor Alpha (RARα) Nuclear Immunostaining in Gliomas and Inability of Retinoic Acid to Influence Neu
CNS-1 was immunoreactive for glial fibrillary acidic protein, S100 protein, vimentin, neural cell adhesion molecule, retinoic acid receptor α, intercellular adhesion molecule, and neuron specific enolase.
RA was previously shown to down-regulate the steady state levels of retinoic acid receptor α (RARα) and retinoid X receptor α (RXRα) in primary brown adipocytes differentiated in culture.