Methods Immunohistochemistry Envision 2-step techniques was used to detect the expression of P63/34βE12 in 70 cases of benign prostatic hyperplasia and 28 cases of prostatic adenocarcinoma,in contrast to P63 and 34βE12 single mark methods.
Objective: To study the possibility of enhanced green fluorescent protein (EGFP) gene as informational gene and select mark in living cell, construct the eukaryotic expression vector pCDNA3.1 (+ ) -EGFP for enhanced green fluorescent protein gene, and transfer the eukaryotic expression vector to Hela cell and rat marrow mesenchymal stem cell,then observe the expression of enhanced green fluorescent protein gene in these two types of cells.
目的:为研究增强型绿色荧光蛋白(enhanced green fluorescent protein,EGFP)基因在活细胞中作为报告基因和筛选标记的可行性,特构建其真核表达载体pCDNA3.1(+)-EGFP,并将它转染至Hela细胞和大鼠骨髓间充质干细胞(mesenchymal stem cell,MSC)中,观察其在这两种细胞中的表达情况。
Results The strong positive rates of the basal cells marked by P63/34βE12 cocktail,P63 and 34βE12 methods are respectively87.1%,60.0% and 41.4%. There is significantly difference between cocktail and single mark methods. The P values are both less than 0.01,with notable significance in the difference of strong positive rates of P63 and 34βE12(P<0.001).
Mark image be decomposed for a series of son image in degree, the second according to noise and different image characteristic, son image be filtered in degree, finally to make wavelet image and gain denosing image.