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scaffolds
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  支架
    Preparation and Properties of Novel Self-Controlled Degradable Chitosan Group Tissue Engineering Scaffolds
    新型自控降解壳聚糖基组织工程支架材料的制备与性能
短句来源
    The results indicated that MC 3T3-E1 cel ls grown on the composite scaffolds showed a higher proliferation rate and spread b etter than that on chitosan scaffolds.
    实验结果表明:MC3T3-E1细胞在纳米级羟基磷灰石/壳聚糖复合支架上粘附铺展良好,其增殖率显著高于培养于纯壳聚糖支架上的细胞。
短句来源
    1 mg scaffold material DNA was limited in 2 μg. The porous chitosan/gelation matrices three-dimensional scaffolds containing pCMV-TGF-β 1 was prepared after twice freeze-dry and the micromorphous of the gene in the material, release and the protection of the material to plasmid DNA were observed.
    1mg支架材料DNA控制在2μg,通过二次冻干的方式制备含有pCMV-TGF-β1的多孔壳聚糖/明胶三维架材料,观测多孔支架材料携带基因成分的微观形态、体外释放特性以及材料对质粒DNA的保护作用。
短句来源
    Four lamellar biological scaffolds were implanted into the other 4 rats separately and taken out after 3 weeks, then they were fixed by 100 g/L neutral formalin, sliced, and stained by haematoxylin and eosin (HE).
    4只大鼠植入其余4片细胞生物支架,植入3周取出,经100g/L中性甲醛固定后,苏木精-伊红染色,切片,观察其组织学变化。
短句来源
    Results The mean adhersive rate was 46.95% of group A,and increased to 60.60% of group B.The osteoblasts in the scaffolds increased with culture time for the two groups,and the speed of group B was faster than group A.
    结果细胞与支架材料平均复合率由胶原处理前的A组46.95%,提高到处理后的B组60.60%;
短句来源
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  支架材料
    Preparation and Properties of Novel Self-Controlled Degradable Chitosan Group Tissue Engineering Scaffolds
    新型自控降解壳聚糖基组织工程支架材料的制备与性能
短句来源
    1 mg scaffold material DNA was limited in 2 μg. The porous chitosan/gelation matrices three-dimensional scaffolds containing pCMV-TGF-β 1 was prepared after twice freeze-dry and the micromorphous of the gene in the material, release and the protection of the material to plasmid DNA were observed.
    1mg支架材料DNA控制在2μg,通过二次冻干的方式制备含有pCMV-TGF-β1的多孔壳聚糖/明胶三维架材料,观测多孔支架材料携带基因成分的微观形态、体外释放特性以及材料对质粒DNA的保护作用。
短句来源
    Results The mean adhersive rate was 46.95% of group A,and increased to 60.60% of group B.The osteoblasts in the scaffolds increased with culture time for the two groups,and the speed of group B was faster than group A.
    结果细胞与支架材料平均复合率由胶原处理前的A组46.95%,提高到处理后的B组60.60%;
短句来源
    Objective To investigate the feasibility of tissue engineered bone construted by using eggshell membrane as scaffolds and loaded with bone marrow mesenchymal stem cells(BMSCs) derived from rat bone marrow as seed cell.
    目的探讨鸡蛋膜作为组织工程支架材料同大鼠骨髓间充质干细胞(bone marrow mesenchy-cmal stem cells,BMSCs)复合培养构建组织工程化骨的可行性。
短句来源
    The second generation cells were seeded on eggshell membrane, and then induced with osteogenic media(50 μg/ml ascorbic acid, 10 mmol/L β-glycerophosphate, and 0.1 μmol/L dexamethasone) for 2 weeks in vitro. The cell-seeded scaffolds were implanted subcutaneously in Wistar rats for 4 weeks.
    将第2代BMSCs同鸡蛋膜支架材料复合,利用含0.1μmol/L地塞米松、10mmol/Lβ-甘油酸钠、50μg/ml维生素C的条件培养液诱导培养2周后,分别将复合材料植入Wistar大鼠右侧背部皮下,左侧植入无细胞复合的鸡蛋膜作为对照。
短句来源
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  “scaffolds”译为未确定词的双语例句
    ②The osteoblasts were loaded onto porous scaffolds consisting of nano-hydroxyapatite/collage (NHAC, 4∶1) and poly (L-lactic acid) (PLLA, 1∶1), porosity > 90%;
    ②采用纳米晶羟基磷灰石-胶原中羟基磷灰石和胶原的比例为4∶1,仿生材料中纳米晶羟基磷灰石和聚左旋乳酸的比例为1∶1;
短句来源
    Although the ultimate strength along the axis of the scaffolds was lower than that of bone samples,but the modulus was only 20% higher than that of the bone samples(P < 0.05).
    长轴方向的强度低于正常松质骨样本,但弹性模量仅比松质骨样本弹性模量高20%(P<0.05)。
短句来源
    The porous calcium phosphate bioceramic scaffolds were fabricated by the polyurethane polymer method and sintered at 850℃ with the Mg (H2PO4) 2(NaPO3) 6 as binder and HA and -TCP powders as raw materials in order to investigate the technique sintered at lower temperature.
    以适量的Mg(H2PO4)2-(NaPO3)6为粘结剂,HA和-TCP粉末为原料,用有机泡沫浸渍法制备钙磷多孔生物陶瓷坯体,并在850℃烧成,探索在较低烧结温度下制备钙磷多孔生物陶瓷的工艺。
短句来源
    Effect of Injectable Scaffolds Material Chitosan- beta-tricalcium Phosphate with Platelet-rich plasma on Proliferation of Bone Marrow Stromal Cells in Vitro
    可注射组织工程材料壳聚糖-β-磷酸三钙/富血小板血浆对体外培养骨髓基质干细胞增殖的影响
短句来源
    Effects of type I collagen on adhesion,proliferation and osteogenic differentiation of rabbit bone marrow stromai cells on PLGA-[ASP-PEG]scaffolds
    Ⅰ型胶原在PLGA-[ASP-PEG]表面修饰对兔骨髓基质干细胞生物力学的影响
短句来源
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  scaffolds
Both SIS scaffolds were then implanted subcutaneously in the dorsa of athymic mice.
      
Subsequently, the modified PLLA scaffolds were co-cultured with the osteoblasts-like in vitro and subcutaneously implanted into nude mice.
      
After a four-week culture in vivo, no significant inflammatory signs were observed in the implanted regions and osteoblast-like congeries with bone-like structure began to form in the scaffolds.
      
Porous apatite biocompatible scaffolds for cell technology for the repair of tissue defects in surgery
      
This paper discusses the manufacturing, chemical, and physical characterization of three-dimensional porous scaffolds developed for the repair of skeletal defects in tissue-engineering applications.
      
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Objective:This experiment aims to find a appropriate scaffold for Tissue Engineer- ing.Methods:The chondrocytes were cultured when they were seeded onto PGA+PLA scaffolds coat- ing with Lecithin(LEC)and Poly-l-lysine(PLYS)together and respectively.With light microscope and scanning electron microscope,the observation of the scaffolds in hydrophilia and adsorptivity to chondrocytes and the function of the cells was made.Results:The PGA scaffolds coating with LEC and PLYS have better hydrophilia...

Objective:This experiment aims to find a appropriate scaffold for Tissue Engineer- ing.Methods:The chondrocytes were cultured when they were seeded onto PGA+PLA scaffolds coat- ing with Lecithin(LEC)and Poly-l-lysine(PLYS)together and respectively.With light microscope and scanning electron microscope,the observation of the scaffolds in hydrophilia and adsorptivity to chondrocytes and the function of the cells was made.Results:The PGA scaffolds coating with LEC and PLYS have better hydrophilia and adsorptivity to the cells;on which the chodrocytes produce more ma- trices.Conclusion:LEC can chang the hydrophilia of the scaffolds;while PLYS can strengthen the ad- sorptivity of the scaffolds;the PGA coating with LEC and PLYS is an ideal scaffold in Tissue Engineer- ing.

目的:通过卵磷脂和多聚赖氨酸分别和共同包埋以聚乳酸固定成形的聚羟基乙酸支架与软骨细胞体外培养,来观察其亲水性和对细胞吸附力的改变以及对细胞功能的影响。方法:将软骨细胞种于上述支架体外培养,通过倒置显微镜及扫描电镜观察支架的亲水性、对细胞的吸附力及基质产生情况。结果:支架以卵磷脂包埋后细胞悬液易浸入到支架内;以多聚赖氨酸包埋后细胞易吸附在支架纤维表面;以卵磷脂和多聚赖氨酸共同包埋后细胞均匀分布于支架纤维之间及吸附在支架纤维表面,且基质产生旺盛。结论:卵磷脂具有增强支架亲水性作用;而多聚赖氨酸除增加支架对细胞的吸附力外,还具有促进细胞功能的作用。以卵磷脂和多聚赖氨酸共同包埋支架可能是组织工程技术中较理想的支架之一。

砄bjective:To investigate the feasibility of using cancellous bone matrix as scaffolds for bone tissue engineering. Methods: The marrow stromal cells of newborn New Zealand rabbits were cultivated and induced in vitro and then seeded into alginate- cancellous bone matrix to form cancellous bone matrix-alginate-marrow stromal osteoblasts composites. The composites were implanted subcutaneously in nude mice. The cancellous bone matrix alone was implanted as control. The osteogenesis was assessed by histological...

砄bjective:To investigate the feasibility of using cancellous bone matrix as scaffolds for bone tissue engineering. Methods: The marrow stromal cells of newborn New Zealand rabbits were cultivated and induced in vitro and then seeded into alginate- cancellous bone matrix to form cancellous bone matrix-alginate-marrow stromal osteoblasts composites. The composites were implanted subcutaneously in nude mice. The cancellous bone matrix alone was implanted as control. The osteogenesis was assessed by histological and roentgenographic analysis. Results: The osteogenesis in the composites was better than that in the cancellous bone matrix. In the composites, both fibrous and cartilaginous osteogenesis were found and the former appeared more obvious. In the control, only slight cartilaginous osteogenesis was found. Conclusion: The cancellous bone matrix can be used as a scaffold material for bone tissue engineering.

目的:观察松质骨基质- 骨髓基质成骨细胞复合移植皮下成骨作用,以探索松质骨基质作为骨组织工程支架材料的可行性。方法:新生兔骨髓细胞体外培养、诱导后,接种于藻酸盐/松质骨基质中,形成松质骨基质/藻酸盐/骨髓基质成骨细胞复合物,并植入裸鼠背部皮下,对照组单纯植入松质骨基质。植入4、8 周后行 X线摄片和组织学检查,观察骨形成情况。结果:松质骨基质/骨髓基质成骨细胞复合物皮下成骨效果明显优于松质骨基质。前者兼有纤维成骨和软骨成骨,以纤维成骨为主;后者仅见少量软骨成骨。结论:松质骨基质可以作为一种良好的骨组织工程支架材料。

Objective:To investigate a method to remove cellular components from bovine pericardial tissue,resulting a scaffold for tissue engineering of heart valve or cardiovascular patch. Methods:A detergent and enzyme extraction was practiced in this study.HE staining was performed to confirm the removal of cells and Von Gieson staining,to show the integrity of collagen and elastin.The changes in tissue shrinkage temperature,and mechanical properties were also studied. Results:The cells were removed...

Objective:To investigate a method to remove cellular components from bovine pericardial tissue,resulting a scaffold for tissue engineering of heart valve or cardiovascular patch. Methods:A detergent and enzyme extraction was practiced in this study.HE staining was performed to confirm the removal of cells and Von Gieson staining,to show the integrity of collagen and elastin.The changes in tissue shrinkage temperature,and mechanical properties were also studied. Results:The cells were removed effectively from bovine pericardial tissue,while the collagen and elastin were kept intact.The mechanical properties remained unaltered.Only the tissue shrinkage temperature dropped insignificantly. Conclusion:The research work demonstrated an effective procedure to remove cells from bovine pericardial tissue while kept its mechanical properties.The acellular matrix needs to be further evaluated biochemically and ultrastructurally.This approach may eventually lead to the engineering of tissue heart valves repopulated with patients' own cells.

目的 :对牛心包材料进行了去污剂—酶联合脱细胞研究 ,为组织工程学方法研制生物瓣提供适合的生物材料。  方法 :用去污剂—酶四步脱细胞方法脱除牛心包组织的细胞。  结果 :该方法脱细胞效果良好 ,且能较好地保持胶原纤维和弹性纤维原有的排列和分布。脱细胞后牛心包材料的厚度、抗拉负荷、伸长率和热皱缩温度只有轻微的减少 ,而抗拉强度没有变化。  结论 :去污剂—酶联合脱细胞法效果良好 ,可用于组织工程的进一步研究。

 
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