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orthopedic department
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  骨科
    SETTING: Orthopedic Department of the First Affiliated Hospital and Department of Anatomy, Jinzhou Medical College.
    单位:锦州医学院附属第一医院骨科和锦州医学院解剖教研室。
短句来源
    METHODS: The experiment was conducted in the laboratory of Orthopedic Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, from February to September 2000. Rat osteoblasts were transfected with pcDNA3-TGF-β1 plasmid by lipofectamine mediated gene transfer, and the plasmid pcDNA transfected cells were set as control group.
    方法:实验于2000-02/09在华中科技大学同济医学院附属协和医院骨科实验室完成。 通过脂质体介导将转化生长因子β1基因导入大鼠成骨细胞,并以质粒pcDNA3转染细胞作为对照。
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  orthopedic department
The authors visited all hospitals within Niigata Prefecture having an orthopedic department and reviewed the medical records and radiographs of all patients who sustained such fractures in 1999.
      
We visited all hospitals within Niigata Prefecture having an orthopedic department and reviewed the medical records and radiographs of all patients who sustained such fractures in 1994.
      
Sixteen patients underwent kyphectomy in the Orthopedic Department of the University of Mainz between 1993 and 1997, all of them supervised by the Neurosurgical Department.
      
This indicates that all emergency trauma films should be reviewed by an experienced radiologist, and an expedient method of informing the orthopedic department of any discrepancies in reading is recommended.
      
This decrease occurred more slowly in the orthopedic department than in the rest of the hospital.
      
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BACKGROUND: Bone marrow stromal cells(BMSCs) are the ideal gene target cells and will have a bright future in the gene therapy of spinal cord injury. OBJECTIVE : To detect the expression of glial cell line- derived neurotrophic factor(GDNF) gene after BMSCs were infected by adenovirus medialed GDNF(Adv GDNF) in vitro and to explore its biological activity. DESIGN:A randomized controlled trial study. SETTING:Laboratory of Orthopedic Department MATERIALS:The experiment was completed in the Laboratory...

BACKGROUND: Bone marrow stromal cells(BMSCs) are the ideal gene target cells and will have a bright future in the gene therapy of spinal cord injury. OBJECTIVE : To detect the expression of glial cell line- derived neurotrophic factor(GDNF) gene after BMSCs were infected by adenovirus medialed GDNF(Adv GDNF) in vitro and to explore its biological activity. DESIGN:A randomized controlled trial study. SETTING:Laboratory of Orthopedic Department MATERIALS:The experiment was completed in the Laboratory of Orthopedic Department,Affiliated Tongji Hospital of Tongji Meidcal College,Huazhong University of Science and Technology.Twenty four SD rats of either gender,weighing(180± 20) g. INTERVENTIONS:BMSCs were infected by Adv GDNF in vitro and then cocultured with spinal cord dorsal root ganglion.The three methods,immunofluorescent chemistry,reverse transcriptase polymerase chain reaction(RT PCR) and enzyme linked immunosorbent assay(ELISA) were used to evaluate GDNF expression in the BMSCs.The biological activity of GDNF was observed by a phase contrast microscope. MAIN OUTCOME MEASURES:Primary outcomes:① RT PCR;② results of immunofluorescent chemical examination;③ biological activity of GDNF in vitro.Secondary outcomes:① culturing and identification of BMSCs;② time effect relationship of GDNF expression revealed by ELISA. RESULTS:Immunofluorescence displayed expression of GDNF in BMSCs 48 hours after Adv GNDF infection.RT PCR analysis demonstrated expression of GDNF mRNA 24 hours after Adv GNDF infection.ELISA confirmed the presence of GDNF in the liquid supernatant of BMSCs 24 hours after Adv GDNF infectionn and showed that GDNF was secreted.The supernatant can promote the neurite outgrowth in the rat dorsal root ganglion(DRG). CONCLUSION:It is demonstrated that BMSCs infected by Adv GDNF can express GDNF steadily and the expressed GDNF has the activity of promoting neurite outgrowth,which lays a foundation of the GDNF gene therapy for spinal cord injury.

背景:骨髓基质干细胞(bonemarrowstromalcells,BMSCs)是外源性目的基因的良好靶细胞,在脊髓损伤的修复中具有良好的应用前景。目的:观察重组腺病毒介导的胶质细胞源性神经营养因子(glialcellline-derivedneurotrophicfactor,GDNF)基因在体外培养的骨髓基质干细胞中的表达,并探讨其生物学活性。设计:以细胞为研究对象,对照观察性研究。单位:一所大学医院骨科实验室。材料:实验于2004-03/06在华中科技大学同济医学院附属同济医院骨科实验室完成。SD大鼠24只,雌雄不限,体质量(180±20)g。干预:用重组腺病毒载体Adv-GDNF感染体外培养的BMSCs,并与脊髓背根神经节共培养。免疫荧光化学的方法检测BMSCs中的GDNF的表达,提取细胞总RNA进行RT-PCR扩增GDNF基因,应用ELISA方法检测其培养上清中的GDNF含量,并通过与脊髓背根神经节共培养观测GDNF的活性。主要观察指标:主要结局:①RT-PCR。②免疫荧光结果。③GDNF的体外活性。次要结局:①BMSCs的培养与鉴定。②ELISA检测蛋白表达与时间的关系。结果:免疫荧光显示Adv-...

背景:骨髓基质干细胞(bonemarrowstromalcells,BMSCs)是外源性目的基因的良好靶细胞,在脊髓损伤的修复中具有良好的应用前景。目的:观察重组腺病毒介导的胶质细胞源性神经营养因子(glialcellline-derivedneurotrophicfactor,GDNF)基因在体外培养的骨髓基质干细胞中的表达,并探讨其生物学活性。设计:以细胞为研究对象,对照观察性研究。单位:一所大学医院骨科实验室。材料:实验于2004-03/06在华中科技大学同济医学院附属同济医院骨科实验室完成。SD大鼠24只,雌雄不限,体质量(180±20)g。干预:用重组腺病毒载体Adv-GDNF感染体外培养的BMSCs,并与脊髓背根神经节共培养。免疫荧光化学的方法检测BMSCs中的GDNF的表达,提取细胞总RNA进行RT-PCR扩增GDNF基因,应用ELISA方法检测其培养上清中的GDNF含量,并通过与脊髓背根神经节共培养观测GDNF的活性。主要观察指标:主要结局:①RT-PCR。②免疫荧光结果。③GDNF的体外活性。次要结局:①BMSCs的培养与鉴定。②ELISA检测蛋白表达与时间的关系。结果:免疫荧光显示Adv-GDNF感染BMSCs48h后即有GDNF的表达,体外培养的BMSCs经Adv-GDNF转染后有GDNF的转录,其培养上清应用ELISA方法分析,在感染24h后即有GDNF的表达,并可持续5~7d的高峰。Adv-GDNF感染的BMSCs的培养液上清可以?

BACKGROUND: The cells of biomaterial compound cytokine gene or compound transfected cytokine gene that are transplanted into the area of bone defect can promote bone repair. OBJECTIVE: To investigate the feasibility of gene therapy for bone defect after rat transforming growth factor (TGF)β1 gene is transfected into osteoblasts. DESIGN: A controlled and observational experiment. SETTING: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. MATERIALS:...

BACKGROUND: The cells of biomaterial compound cytokine gene or compound transfected cytokine gene that are transplanted into the area of bone defect can promote bone repair. OBJECTIVE: To investigate the feasibility of gene therapy for bone defect after rat transforming growth factor (TGF)β1 gene is transfected into osteoblasts. DESIGN: A controlled and observational experiment. SETTING: Department of Orthopedics, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology. MATERIALS: Five newborn Sprague-Dawley rats of either gender were included. METHODS: The experiment was conducted in the laboratory of Orthopedic Department, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, from February to September 2000. Rat osteoblasts were transfected with pcDNA3-TGF-β1 plasmid by lipofectamine mediated gene transfer, and the plasmid pcDNA transfected cells were set as control group. The transient expression of TGF-β1 was detected by strept avidin-biotin-peroxidase complex (SABC) method and in situ hybridization detection 24 hours later. The cells transfected by G418 for 2 weeks were detected with SABC to investigate the stable expression of TGF-β1 gene. MAIN OUTCOME MEASURES: SABC method and in situ hybridization detection were applied to detect gene expression. RESULTS: ①The immunohistochemical detection of transient expression of pcDNA3-TGF-β1 transfected osteoblasts and in situ hybridization detection: After the osteoblasts were transfected for 24 hours, cytoplast was full of brown granules and there were no brown granules in the cytoplast of blank carrier transfected cells, indicating that TGF-β1 mRNA was significantly increased in transgeneic cells. ②Transgeneic cell detection through G418: transfected cells still had high expression of TGF-β1 after 2-week G418 screening. CONCLUSION: Osteoblasts can express cytokine immediately with high effect using gene transfection technique. The stable and high expression is presented after TGF-β1 gene is transfected into osteoblasts at the moment of transfection and after 2-week screening, proving the feasibility of gene therapy for bone defect when cytokine gene is transfected into osteoblasts.

背景:将生物材料复合细胞因子基因,或复合转入细胞因子基因的细胞植入骨缺损处可以促进骨修复。目的:观察将大鼠转化生长因子β1基因转染成骨细胞后进行骨缺损基因治疗的可行性。设计:对照观察实验。单位:华中科技大学同济医学院附属协和医院骨科。对象:新生SD大鼠5只,雌雄不限。方法:实验于2000-02/09在华中科技大学同济医学院附属协和医院骨科实验室完成。通过脂质体介导将转化生长因子β1基因导入大鼠成骨细胞,并以质粒pcDNA3转染细胞作为对照。转染24h后通过链霉亲和素-生物素化过氧化物酶复合物法和原位杂交法检测目的基因瞬时表达的情况。采用G418筛选转染细胞2周,获得阳性细胞克隆,用链霉亲和素-生物素化过氧化物酶复合物法检测转染细胞稳定表达转化生长因子β1的情况。主要观察指标:链霉亲和素-生物素化过氧化物酶复合物法和原位杂交法检测转染细胞基因表达情况。结果:①pcDNA3-TGF-β1转染成骨细胞瞬时表达组化检测和原位杂交检测结果:24h转染成骨细胞胞浆中充满染色的棕黄色颗粒,对照组空载体转染细胞胞浆中则没有棕黄色颗粒,说明转基因细胞中转化生长因子β1mRNA明显增高。②G418筛选转基因细胞组化检测:G418...

背景:将生物材料复合细胞因子基因,或复合转入细胞因子基因的细胞植入骨缺损处可以促进骨修复。目的:观察将大鼠转化生长因子β1基因转染成骨细胞后进行骨缺损基因治疗的可行性。设计:对照观察实验。单位:华中科技大学同济医学院附属协和医院骨科。对象:新生SD大鼠5只,雌雄不限。方法:实验于2000-02/09在华中科技大学同济医学院附属协和医院骨科实验室完成。通过脂质体介导将转化生长因子β1基因导入大鼠成骨细胞,并以质粒pcDNA3转染细胞作为对照。转染24h后通过链霉亲和素-生物素化过氧化物酶复合物法和原位杂交法检测目的基因瞬时表达的情况。采用G418筛选转染细胞2周,获得阳性细胞克隆,用链霉亲和素-生物素化过氧化物酶复合物法检测转染细胞稳定表达转化生长因子β1的情况。主要观察指标:链霉亲和素-生物素化过氧化物酶复合物法和原位杂交法检测转染细胞基因表达情况。结果:①pcDNA3-TGF-β1转染成骨细胞瞬时表达组化检测和原位杂交检测结果:24h转染成骨细胞胞浆中充满染色的棕黄色颗粒,对照组空载体转染细胞胞浆中则没有棕黄色颗粒,说明转基因细胞中转化生长因子β1mRNA明显增高。②G418筛选转基因细胞组化检测:G418筛选2周后的转染细胞仍然有较高的转化生长因子β1表达。结论:利用基因转染技术可使成骨细胞瞬时、高效表达细胞因子,转染后瞬时和筛选2周后,均呈现转化生长因子β1基因转染成骨细胞后稳定的高表达,说明采用细胞因子基因转染成骨细胞进行骨缺损的基因治疗具有可行性。

BACKGROUND: Anterior and dorsal root nerve tracts should be separated to small tracts in high selective dorsal rhizotomy, because detailed tract separation will benefit electrostimulation, thereby helping correctly cutting the lower-threshold Ia nerve fibers that cause convulsion, and meanwhile sensory nerve fibers in dorsal nerve root can be reserved as many as possible. OBJECTIVE: To meet the needs of limited and high selective spinal dorsal rhizotomy, anterior and dorsal root of spinal nerve were microanatomized...

BACKGROUND: Anterior and dorsal root nerve tracts should be separated to small tracts in high selective dorsal rhizotomy, because detailed tract separation will benefit electrostimulation, thereby helping correctly cutting the lower-threshold Ia nerve fibers that cause convulsion, and meanwhile sensory nerve fibers in dorsal nerve root can be reserved as many as possible. OBJECTIVE: To meet the needs of limited and high selective spinal dorsal rhizotomy, anterior and dorsal root of spinal nerve were microanatomized to be certain of the separation standard and the number of small nerve tracts, so as to provide reliable basis and novel operative standard for clinical operation. DESIGN: Single sample experiment with adult corpses as subjects. SETTING: Orthopedic Department of the First Affiliated Hospital and Department of Anatomy, Jinzhou Medical College. PARTICIPANTS: This study was carried out at the Anatomical Laboratory of Jinzhou Medical College in December 1999. Fifteen adult corpses, 11 males and 4 males, were donated, and the donators signed informed consent when alive. METHODS: ① The anterior and dorsal roots of spinal nerves were obtained from the 15 adult spinal cords (30 sides) and subjected to morphological observation and micro-measurement at the level of L1-S2. ② The anterior and dorsal roots of L5 spinal cord were obtained from a fresh corpse for immunohistochemical staining. The starting part, middle part and the exterior of intervertebral foremen was cut into slices, and the total number of nerve fibers, the number of Ia nerve fibers responsible for convulsion, and their percentage in the total fibers were counted. Meanwhile the distribution and the number of Ia nerve fibers in the three parts were compared. MAIN OUTCOME MEASURES: ① The nerve tract separation in spinal nerve root and the diameter of small nerve tract. ② The total number of nerve fibers per 100 μm2, the percentage of Ia nerve fibers in the total nerve fibers at the starting part, middle part and exterior of intervertebral foremen of spinal nerve dorsal root. RESULTS: ① Nerve root at spinal cord pyramidal part was converged by root filaments. Microsurgical observation proved that dorsal root could be divided into 10-18 small tracts and anterior root 6-11 tracts; the diameter of small tracts was similar and the number was constant. ② The total number of nerve fibers in the three parts of spinal nerve dorsal root was (3 243±143) fibers per 100 μm2, including (1 702±85) Ia nerve fibers that constituted about 52.5% of the total nerve fibers. Ia nerve fibers were found to be evenly distributed in dorsal root and no gathering could be observed. CONCLUSION: The tracts in anterior and dorsal roots of spinal cord should be separated as minutely as possible during improved dorsal rhizotomy, which is beneficial for cutting off Ia nerve fibers correctly. Generally, the anterior root consists of 6-11 small tracts and dorsal roots of 10-18 small tracts, and nerve tracts should not be cut off over 1/2 of total dorsal nerve fibers.

背景:高选择性脊神经后根部分切断术中前后根的神经分束应达到神经小束水平,分束越多越利于电刺激选择,利于准确地切断阈值低的引起痉挛的Ia类神经纤维,也越可能最大限度地保留后根中的感觉神经纤维。目的:根据限制性和高选择性脊神经后根切断术的要求,对脊神经前后根进行显微解剖,确定神经小束的分束标准和数目,为临床手术提供可靠的依据和新的手术标准。设计:以成人尸体标本为观察对象,单一样本实验。单位:锦州医学院附属第一医院骨科和锦州医学院解剖教研室。对象:实验于1999-12在锦州医学院解剖学实验室进行。以志愿捐献的15具成人尸体标本为观察对象,男11具,女4具,生前均签署志愿捐献书。方法:①在15具(30侧)成人脊柱标本上,对L1~S2节段的脊神经前后根进行形态学观察和显微测量。②取新鲜尸体的L5脊神经前后根进行免疫组化染色,将脊神经后根起始部、中间部和椎间孔外部3个部位切片,分别测定神经纤维总数、引起痉挛的Ia类神经纤维的数目及其占神经纤维总数的百分率,比较3个部位Ia类神经纤维的分布规律和数量。主要观察指标:①脊神经根神经分束情况及神经小束的直径。②脊神经后根起始部、中间部和椎间孔外部计数100μm2神经纤维总数及I...

背景:高选择性脊神经后根部分切断术中前后根的神经分束应达到神经小束水平,分束越多越利于电刺激选择,利于准确地切断阈值低的引起痉挛的Ia类神经纤维,也越可能最大限度地保留后根中的感觉神经纤维。目的:根据限制性和高选择性脊神经后根切断术的要求,对脊神经前后根进行显微解剖,确定神经小束的分束标准和数目,为临床手术提供可靠的依据和新的手术标准。设计:以成人尸体标本为观察对象,单一样本实验。单位:锦州医学院附属第一医院骨科和锦州医学院解剖教研室。对象:实验于1999-12在锦州医学院解剖学实验室进行。以志愿捐献的15具成人尸体标本为观察对象,男11具,女4具,生前均签署志愿捐献书。方法:①在15具(30侧)成人脊柱标本上,对L1~S2节段的脊神经前后根进行形态学观察和显微测量。②取新鲜尸体的L5脊神经前后根进行免疫组化染色,将脊神经后根起始部、中间部和椎间孔外部3个部位切片,分别测定神经纤维总数、引起痉挛的Ia类神经纤维的数目及其占神经纤维总数的百分率,比较3个部位Ia类神经纤维的分布规律和数量。主要观察指标:①脊神经根神经分束情况及神经小束的直径。②脊神经后根起始部、中间部和椎间孔外部计数100μm2神经纤维总数及Ia类神经纤维占神经纤维总数的百分率。结果:①脊髓圆锥部脊神经根是由根丝逐步汇合而成。应用显微外科技术,后根一般可分为10~18小束,前根一般分为6~11小束,其小束的直径是基本一样,数值较为恒定。②脊神经后根起始部、中间部和椎间孔外部计数100μm2总的神经纤维数为(3243±143)根,Ia类神经纤维为(1702±85)根,占总神经纤维数的52.5%。Ia类神经纤维在后根内呈均匀分布,没有集中分布区。结论:改良脊神经后根部分切断术的最大特点即脊神经前后根的分束标准应尽量细,这样有利于准确切断Ia类神经纤维,一般前根达到6~11小束,后根达到10~18小束,切断最大比例应不超过后根神经纤维总数的1/2。

 
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