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finger print     
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  指纹图谱
     Study on the Modern Preparation of SFPA Injection and the Quality Control of the HPLC Fingerprint Chromatography
     现代化中药SFPA注射液及其HPLC指纹图谱法质量控制的研究
短句来源
     Studies on the Establishment of HPLC Fingerprint of Agrimonia Pilosa Ledeb and Its Antitumor Mechanisms
     仙鹤草指纹图谱的建立及其抗肿瘤作用机制研究
短句来源
     AN INITIAL STUDY ON MINK DNA FINGERPRINTINGI.ANALYSING OF DNA FINGERPRINT
     水貂DNA指纹的研究Ⅰ.不同遗传群体DNA指纹图谱特征分析
短句来源
     To study on flavonoids of traditional Chinese liquid medicine-Fructus Polygoni Orientalis. HPLC system was used to obtain the fingerprint spectrum of Quercetin of Fructus Polygoni Orientalis from 9 places of China. The chromatography condition was DiamonsilTMC18 column(250 mm×4.6 mm,5 μm),mobile phase Methanol-0.2%H3PO4,and flow speed 1.0 mL/min,detection wavelength 310 nm.
     对红蓼果实水红花子的黄酮类成分进行研究,采用HPLC色谱法对分布于我国9个地区的水红花子的有效部位槲皮素进行指纹图谱研究,色谱条件:DiamonsilTMC18柱(250 mm×4.6 mm,5μm),流动相为甲醇-0.2%磷酸水溶液梯度洗脱,流速1.0 mL/min,检测波长为310 nm。
短句来源
     METHODS: Using the method of X-ray diffraction fourier fingerprint patterns for the analysis and calculation of 12 habitats of Herba Ephedrae.
     方法:采用X射线衍射Fourier指纹图谱分析法,分析计算了12产地麻黄草样品的X射线衍射Fourier指纹图谱
短句来源
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  指纹
     AFLP Fingerprint analysis and Genetic Relationship among Eco-types of G.soja and G.max in China
     中国野生大豆与栽培大豆AFLP指纹分析及生态群体遗传关系研究
短句来源
     Study on Structure-based Fingerprint Representation and Matching Algorithm
     基于结构的指纹表达及其匹配算法研究
短句来源
     Research on Biological Recognition Technology Based on Fingerprint and Iris
     基于指纹与虹膜生物识别技术研究
短句来源
     Fingerprint Classification and Fingerprint Matching Based on Embedded Hidden Markov Model Model
     基于嵌入式隐Markov模型的指纹分类和匹配研究
短句来源
     Research on the Technologies of Feature Extraction and Recognition for Low Quality Fingerprint Image
     低质量指纹图像的特征提取与识别技术的研究
短句来源
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  指纹
     AFLP Fingerprint analysis and Genetic Relationship among Eco-types of G.soja and G.max in China
     中国野生大豆与栽培大豆AFLP指纹分析及生态群体遗传关系研究
短句来源
     Study on Structure-based Fingerprint Representation and Matching Algorithm
     基于结构的指纹表达及其匹配算法研究
短句来源
     Research on Biological Recognition Technology Based on Fingerprint and Iris
     基于指纹与虹膜生物识别技术研究
短句来源
     Fingerprint Classification and Fingerprint Matching Based on Embedded Hidden Markov Model Model
     基于嵌入式隐Markov模型的指纹分类和匹配研究
短句来源
     Research on the Technologies of Feature Extraction and Recognition for Low Quality Fingerprint Image
     低质量指纹图像的特征提取与识别技术的研究
短句来源
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  指印
     Preliminary study on extraction of DNA from sweat fingerprint
     汗潜指印DNA提取方法的初步研究
短句来源
     Methods Using Chelex-100 and Microcon-100 to extract DNA from sweat latent fingerprint has been established.
     方法采用Chelex-100和Microco-100浓缩柱,提取汗潜指印中DNA,STR复合扩增荧光电泳检测。
短句来源
     Compare with the three methods of DNA extraction of sweat latent fingerprint on the adhesive side of tapes
     3种提取胶带粘面汗潜指印中DNA的方法比较
短句来源
     Study on the experiment about the sweat fingerprint showing techniques with ultraviolet ray luminous in permeability object
     渗透性客体上潜在汗液指印紫外发光显现技术的实验研究
短句来源
     STR typing of sweat latent fingerprint
     汗潜指印的STR分型检测
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  finger print
A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation.
      
In addition, it was observed that the element pattern of textile samples resembled 'finger print type', TXRF-spectra.
      
In addition, there are vermicular, 'finger print' intergrowths of nepheline with potassium feldspar, and patchy to micrographic intergrowths of kalsilite with potassium feldspar.
      
All of these detail leaf features show specific specificity of leave finger print for 6 rhododendrons.
      
Finger print analysis of bacterial 16S RNA (molecular phylogeny) has not only changed the classification of bacteria but also the approach to solving environmental problems.
      
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1. By treatment with sodium sulfite and sodium tetrathionate, the disulfide linkages in theinsulin molecule were split into S-sulfonate groups. From the split products, pure A and B chainswere obtained by ion-exchange chromatography or by zone electrophoresis on cellulose powder. 2. The S-sulfonates of A and B chains were completely inactive in the mouse-convulsion test.After reduction of the S-sulfonate groups by excess thioglycolate to sulfhydryl groups followed byaerobic oxidation at pH 8.5, neither pure...

1. By treatment with sodium sulfite and sodium tetrathionate, the disulfide linkages in theinsulin molecule were split into S-sulfonate groups. From the split products, pure A and B chainswere obtained by ion-exchange chromatography or by zone electrophoresis on cellulose powder. 2. The S-sulfonates of A and B chains were completely inactive in the mouse-convulsion test.After reduction of the S-sulfonate groups by excess thioglycolate to sulfhydryl groups followed byaerobic oxidation at pH 8.5, neither pure A chain nor pure B chain alone gave active product, butwhen reduced A and B chains were reoxidized together at pH 8.5, considerable insulin activitywas regenerated. The activity recovered was usually about 5-10% of the activity of crystallineinsulin. 3. Regeneration of insulin activity also took place when the reduced form of one of the chainswas incubated with the S-sulfonate of the other chain. 4. Purification of the active, reoxidized product resulted in a crystalline material which had aspecific activity of 18.4 international units/mg in the mouse-convulsion test and possessed the samehypoglycemic activity as insulin. It was indistinguishable in crystal shape from insulin (Fig. 7),and had the same electrophoretic property (Fig. 9) as well as the same R_F values in three solventsystems (Fig. 10) as those of crystalline insulin. 5. By two-dimensional paper electrophoresis-paper chromatography the peptic digests of crystal.line insulin and of this crystalline material gave essentially the same "finger-prints" (Fig. 11).

(1)用亚硫酸盐及四硫硫酸盐将胰岛素的硫-硫键拆成S-磺酸基后,再经过离子交换或板型电泳分离,可以得到纯的A及B链。(2)用小白鼠惊厥法检查,S-磺酸型A及B链都不具有生物活力。用过量巯基乙酸将纯的A或B链的S-磺酸基还原为巯基,然后分别将还原型的A或B链溶液(pH 8.5)单独在空气中氧化也不产生活力。但将还原型A及B链混合,在同样条件下氧化时,可以获得有活力的产物。活力一般恢复到原活力的5-10%左右。(3)用还原型的A或B链分别与S-磺酸型的B或A链共同保温都可以得到有活力的产物。(4)将恢复活力的重氧化产物进行提纯的结果得到比活力为每毫克18.4国际单位的结晶。这样所获得的晶体其结晶形状、降血糖性质、电泳性质以及在三种溶剂系统中的比移都和结晶胰岛素相同。(5)以上晶体和结晶胰岛素的胃蛋白酶酶解图谱也基本上相同。

The enzymatic synthesis of the hexanucleotide fragment GpCpm~1IpΨpGpGp(2'or3')corresponding to the anticodon region of yeast alanine tRNA from ~32pGpGp(2'or3')and GpCpm~1IpΨ by T_4 RNA ligase is described.It has been found that under the general experimental conditions(incubation at37℃ for 2 hours),the yield of the ligation product is only about 10%.Howeverincubation at a lower temperature(10℃)for 48 hours gives the desired hexanucleotidein good yield(50~60%).The reaction mixture containing 20 mM MgGl_2,3.3mM...

The enzymatic synthesis of the hexanucleotide fragment GpCpm~1IpΨpGpGp(2'or3')corresponding to the anticodon region of yeast alanine tRNA from ~32pGpGp(2'or3')and GpCpm~1IpΨ by T_4 RNA ligase is described.It has been found that under the general experimental conditions(incubation at37℃ for 2 hours),the yield of the ligation product is only about 10%.Howeverincubation at a lower temperature(10℃)for 48 hours gives the desired hexanucleotidein good yield(50~60%).The reaction mixture containing 20 mM MgGl_2,3.3mM DTT,bovine serumalbumin(10μg/ml),1.5mM ATP,50mM glyeylglycine buffer(pH8.3),0.5mMGpCpm~1IpΨ,0.1mM ~32pGpGp(2'or3')and RNA ligase(300U/ml)was kept at 10℃ for48 hours.The yield was 50~60% based on the donor.The ligation product was sepa-rated byDEAE-Sephadex A-25 column chromatography to give pure GpCpm~1IpΨ~32pGpGp(2'or 3').The purity and the correct sequence of the hexanuclcotide were confirmed bytwo-dimensional electrophoresis & homochromatography,phosphatase resistance,nearest neighbour analysis and finger-print analysis after pancreatic RNase digestion.

本文报导了酵母丙氨酸转移核糖核酸3′-半分子反密码区中GpCpm~1IppGpGp的酶促合成。作者发现,当以~(32)pGpGp(2′,3′)为供体,GpCpm~1Ip为受体,用T_4RNA 联结酶在一般条件下联结时(37℃,2小时),产率只有10%左右;但在10℃条件下,供体与受体比为1:5时,可以得到50~60%的较高产率。将~(32)pGpGp(2′,3′)与GpCpm~1lp用T_4RNA 联结酶在pH8.3,10℃下反应48小时后,再经DEAE-葡聚糖凝胶A-25柱层析分离纯化,即可得到GpCpm~1Ip~(32)pGpGp(2′,3′)。产物经双向电泳层析图谱分析,抗单酯酶测定,毗邻分析,用牛胰核糖核酸酶水解然后5′端用~(32)p 标记,再经双向图谱和核苷酸组成比例等鉴定,证明产物的纯度和排列顺序符合预定要求。

More than one hundred cases of Hemoglobin E have been reported in china since1964.Most of these propositi were heterozygotes,but a few cases were homozygotesor double heterozygotes of HbE and thalassemia(βE/βo and βE/β~+).They mainlybelonged to the Han(汉) nationality,Zhuang(壮) and Dai(傣) ethnic groups etc,witha wide distribution in 8 provinces.Structure analyses were made in some of themand one case with HbE was found from Meng(蒙) ethnic group,However,there wasno information on this Hb variant among Yao(瑶) ethnic...

More than one hundred cases of Hemoglobin E have been reported in china since1964.Most of these propositi were heterozygotes,but a few cases were homozygotesor double heterozygotes of HbE and thalassemia(βE/βo and βE/β~+).They mainlybelonged to the Han(汉) nationality,Zhuang(壮) and Dai(傣) ethnic groups etc,witha wide distribution in 8 provinces.Structure analyses were made in some of themand one case with HbE was found from Meng(蒙) ethnic group,However,there wasno information on this Hb variant among Yao(瑶) ethnic group(another one of ournational minorities)ever obtained in China.So the following case with HbE presentedhere is the first one.This female propositus,a 14 years-old girl of Yao ethnic group,was a nativeof Jiang-hua County,Hunan Province.She and her mother carrying the sameabnormal Hb were all heterozygotes.Hematologic examinations gave a positiveisopropanol precipitation test and(+)inclusion bodies.Clinically she was symptomless,Primary structure analysis was performed and a substitution of lysine for the 26thglutamic acid from the N-terminal end of β-Chain was identified[denoted as β26(B8)Glu→Lys].Based on her“finger-print”of β~XⅠ peak,it is presumably considered thatδ chain may be mixed with some β~E chain.

1979年迄今,在我国汉、壮和傣族中已发现多倒 HbE,还有1例蒙族 HbE。本文报道的这例 HbE,为我国瑶族发现的第一例,已经过一级结构分析确证。先证者女性,14岁,瑶族,湖南江华县人。家系调查,证明其母携者相同的异常 Hb,两人均属杂合子型。血液学检查,异丙醇试验阳性,保温12小时包涵体阳性。无临床症状。异常珠蛋白链的一级结构分析,显示其β链 N 端的第26位谷氨酸被赖氨酸所取代〔可表示如β26(B8)Glu→Lys〕。根据其β~xI 的指纹图,我们推测δ链可能混有少许β~E 链。

 
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