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finger print
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  finger print
A quantitative estimation of metabolites showed a strain specific (Finger print) metabolite profile which can be used for strain/species identification/differentiation.
      
In addition, it was observed that the element pattern of textile samples resembled 'finger print type', TXRF-spectra.
      
In addition, there are vermicular, 'finger print' intergrowths of nepheline with potassium feldspar, and patchy to micrographic intergrowths of kalsilite with potassium feldspar.
      
All of these detail leaf features show specific specificity of leave finger print for 6 rhododendrons.
      
Finger print analysis of bacterial 16S RNA (molecular phylogeny) has not only changed the classification of bacteria but also the approach to solving environmental problems.
      
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In this paper,differential proteomic method was employed to study the molecular mechanism of crop allelopathy.The traditional QTL method was also compared.Experiments were conducted to determine the molecular mechanism of rice allelopathy under the biotic stresses induced by barnyardgrass(Echinochloa crusgalli L.).One of the two cloning tillers,obtained from single rice plant at 5 leaf-stage,was cultivated in pots with the addition of root exudates of either rice or barnyardgrass,respectively.At 7d,their leaf...

In this paper,differential proteomic method was employed to study the molecular mechanism of crop allelopathy.The traditional QTL method was also compared.Experiments were conducted to determine the molecular mechanism of rice allelopathy under the biotic stresses induced by barnyardgrass(Echinochloa crusgalli L.).One of the two cloning tillers,obtained from single rice plant at 5 leaf-stage,was cultivated in pots with the addition of root exudates of either rice or barnyardgrass,respectively.At 7d,their leaf proteins were extracted for two-dimensional electrophoresis(2-DE).More than 800 proteins were resolved in each 2-DE gel and four leaf proteins displayed differentially.The four proteins showed high degree of reproducibility in the allelopathic rice response to the stresses of barnyardgrass.Individual protein spots were excised respectively from the 2-DE gel and measured by MALDI-TOF MS.Their Peptide Mass Finger Prints(PMF) were obtained.Further SWISS-PROT database search identified four matched proteins: peroxidase 2 precursor(POD),phenylalanine ammonia-lyase(PAL),3-hydroxy-3-methylglutaryl-coenzyme A reductase 3(HGMR) and thioredoxin M-type(Trx-m).The genes ecoding these four differential proteins were located on the chromosome 4,7,8,and 12 of rice.The QTLs of rice allelopathy varied with the donor plants,receiver plants and bioassay methods used.QTL analysis based on its approximate map locations and its small effects on phenotype proved to be inefficient.Advances in proteomics provide opportunities for accurate identification of positional,functional and expressional genes.By comparison of 2-DE protein patterns obtained from the treated and untreated plants,a set of stress-responsive proteins encoded by expressional candidate genes could be identified.Sequencing of these stressed-responsive proteins will then reveal the gene functions associated with the stress tolerant trait.The encoding genes will thus be regarded as both expressional and functional candidate genes.

试验旨在分析运用分子标记技术(QTL)和差异蛋白组学技术研究作物化感作用分子机理的差异性。首先运用差异蛋白组学技术探讨在生物胁迫(稗草)下水稻化感作用潜力变化的内在分子机理。分别用稗草和水稻的根系分泌物培养切自一株5叶龄化感水稻P I312777植株并经恢复的2个分蘖。7d后,提取处理和对照相同叶位叶片的全蛋白质并进行双向电泳,每张电泳胶片上获得800多个电泳胶点,其中差异表达的蛋白质点有4个。采用M ALD I-TOF-M S对各差异蛋白质点进行肽质量指纹图谱分析,经过SW ISS-PROT数据库查询,结果表明化感水稻P I312777在稗草胁迫下的特异蛋白分别与苯丙氨酸氨解酶(PAL)、硫还原型蛋白(T rx-m)、3-羟基-3-甲基戊二酰辅酶A还原酶(HM GR)和过氧化物酶(POD)相匹配。根据编码以上4个差异蛋白质的DNA序列,发现编码以上4个差异蛋白的基因分别位于水稻染色体4、7、8和12上的特定克隆位点,这就是与化感作用相关基因。前人也运用QTL方法开展作物化感作用的分子机理研究,但由于所采用的供体材料、受体植物及对表型性状的评价方法等的不同,定位结果存在较大的差异。综合比较两种方法后认为,运用...

试验旨在分析运用分子标记技术(QTL)和差异蛋白组学技术研究作物化感作用分子机理的差异性。首先运用差异蛋白组学技术探讨在生物胁迫(稗草)下水稻化感作用潜力变化的内在分子机理。分别用稗草和水稻的根系分泌物培养切自一株5叶龄化感水稻P I312777植株并经恢复的2个分蘖。7d后,提取处理和对照相同叶位叶片的全蛋白质并进行双向电泳,每张电泳胶片上获得800多个电泳胶点,其中差异表达的蛋白质点有4个。采用M ALD I-TOF-M S对各差异蛋白质点进行肽质量指纹图谱分析,经过SW ISS-PROT数据库查询,结果表明化感水稻P I312777在稗草胁迫下的特异蛋白分别与苯丙氨酸氨解酶(PAL)、硫还原型蛋白(T rx-m)、3-羟基-3-甲基戊二酰辅酶A还原酶(HM GR)和过氧化物酶(POD)相匹配。根据编码以上4个差异蛋白质的DNA序列,发现编码以上4个差异蛋白的基因分别位于水稻染色体4、7、8和12上的特定克隆位点,这就是与化感作用相关基因。前人也运用QTL方法开展作物化感作用的分子机理研究,但由于所采用的供体材料、受体植物及对表型性状的评价方法等的不同,定位结果存在较大的差异。综合比较两种方法后认为,运用差异蛋白组学技术分析水稻化感作用的分子机理,比QTL技术更加直接和深入。因为比较胁迫处理和对照植物组织的2-DE图谱将能鉴定出由表达候选基因编码的胁迫蛋白质,氨基酸残基序列的测定将揭示那些功能与胁迫性状密切相关的蛋白质,这种编码的基因就是兼具功能与表达的候选基因。

Analysis of the Esterase isozyme of black rices variety was carried out by PAGE,The results showed that the Esterase isozyme had a plenty of polymorphism on account of high resolution.Each variety of black rices has it's own unique band pattern.Compared with their finger prints,the results showed their bands reach 15 at most.a_1 and a_3 bands are common for five varieties.Analysis of similarity degree index of EST Isozyme indicated that three varieties had a near relationship becanse of a high index which...

Analysis of the Esterase isozyme of black rices variety was carried out by PAGE,The results showed that the Esterase isozyme had a plenty of polymorphism on account of high resolution.Each variety of black rices has it's own unique band pattern.Compared with their finger prints,the results showed their bands reach 15 at most.a_1 and a_3 bands are common for five varieties.Analysis of similarity degree index of EST Isozyme indicated that three varieties had a near relationship becanse of a high index which is above 0.75.

采用改进的聚丙烯酰胺凝胶电泳对5种黑稻主要栽培品种进行了EST同工酶谱分析。结果表明,此方法分辨率高。测试各品种的EST同工酶存在丰富的多态性,其电泳图谱具有鲜明的特征,并构成了各自的“指纹”。其中黑优粘和云黑酶谱条带数多达15条,a1和a3为5个品种的共有谱带。通过EST同工酶谱相似度指数分析,黑优粘与黑丰糯、乌贡1号相似度指数大于0.75,说明这3个品种间亲缘关系较近。

>=RAPD (Random Amplified Polymorphic DNA) is a new kind of molecular marking technique. The principle, character and the application of RAPD in the investigation of genetic diversification and stability of tea plant, relationship between the tea plant and other related plants , identification of tea cultivar and their relationship, the RAPD marking related with tea characters and the preparation of DNA finger-print atlas of tea clones were reviewed and discussed by the authors.

随机扩增多态性DNA(Random Amplified Polymorphic DNA,RAPD)是一种类型的分子标记,它能从DNA分子水平直接反映出整个基因组的特征,快速准确地检测基因组DNA序列的多态性,并且这种技术具备成本低廉、操作简单灵敏、分析速度快、无放射性污染、需要极微量的DNA以及产生丰富的多态性等优点,短短几年已在生命科学的各个领域得到广泛的应用。 本文简要介绍了RAPD的原理、特点,以及最近几年来国内外(主要是日本、肯尼亚和中国)学者应用RAPD技术在茶树遗传多样性、茶树的起源与传播、茶树栽培品种及其近缘植物的系统学分类、茶树品种鉴别与亲子鉴定、与茶树性状连锁的RAPD标记、茶树优质资源的遗传稳定性、茶树无性系良种的DNA指纹图谱研究等诸多方面取得的令人鼓舞的成就。

 
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