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    Screening Target-Protein Gene of Artemisinin Antimalarials Using Drug-Western Methoed from cDNA Expression Library
    应用Drug-western法从cDNA表达文库中筛选青蒿素类抗疟药靶标蛋白基因
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    Construction and Screening of the cDNA Subtractive Library of Aloe Vera L., and the Induced Expression of the Gene for NADP-Malic Enzyme under Salt Stress
    盐胁迫下库拉索芦荟cDNA消减文库的构建筛选及NADP-苹果酸酶基因的盐诱导表达
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    Construction of Silky BAC Library and Study of Melanin-related Gene TYRP1 and ID
    丝羽乌骨鸡BAC文库的构建和黑色素相关基因TYRP1和ID的研究
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    Effects of Overexpression of Suaeda Salsa cDNA Library and Co-overexpression of SsNHX1-AVP1 on Salt Tolerance in Arabidopsis Thaliana Cloning and Expression of FAD7 Gene of Suaeda Salsa
    过量表达盐地碱蓬cDNA文库及SsNHX1-AVP1对拟南芥耐盐性的影响和盐地碱蓬FAD7基因cDNA的克隆与表达分析
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    Study on Factors Interacted with Angiotensin-(1-7) from Rat Cardiac Myocyte cDNA Library by Yeast Two-hybrid System
    用酵母双杂交系统从大鼠心肌细胞cDNA文库中筛选与血管紧张素-(1-7)相互作用的因子
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  “library”译为未确定词的双语例句
    Cloning of the Undiftercntiated Immunoglobulin Heavy Chain Variable Region Gene from the New Born Mouse Gene Library(BALB/c)Mouse
    小鼠(BALB/c)未分化型免疫球蛋白重链可变区(V_H)基因的克隆
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    Isolation and Organization Analysis of Histone H, Genes from a Rice Genomic Library
    水稻组蛋白H_3基因的分离和组织结构分析
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    Construction of Basidiomycetes LMPQ39 Pst Ⅰ Fragment Genomic Library
    担子菌LMPQ39 Pst Ⅰ片段基因库的构建
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    PRELIMINARY STUDY ON CLONING OF cDNA GENE CODING FOR NEW ZINC FINGER PROTEINS EXPRESSED IN K562 CELLS cCDNA LIBRARY
    从K562细胞中克隆新的锌指蛋白cDNA基因的初步研究
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    A 613bp cDNA screened from a human tonsil lambda gtll cDNA library by a mixture of McAb 5C5 and 5C5-G1
    用单抗5C5和5C5-G1筛选的613bp cDNA的核苷酸序列分析
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A library of 363 glycoconjugates and C-nucleosides synthesized by our earlier reported methods were screened for their effect on isolated filarial glutamate cysteine ligase (GCL) and glutathione reductase (GR).
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The library has been tested against Leishmania donovani and Plasmodium falciparum, with activity at the micromolar level.
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Aptamers which specifically recognize targets are selected from random oligonucleotide library using systematic evolution of ligands by exponential enrichment (SELEX).
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Screening of Peptide Inhibitors of TACE from a Phage Display Random 15-Peptide Library by Recombinant TACE Ectodomain
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In the present study, a 15-peptide library was used to identify potential TACE antagonists.
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         Although immunoglobulin IgE has an imoortant biological function as the mediator of allergic reaction, we know little about the genetics of the Cε gene. For its further study we have screened and isolated the Cε gene from the new born mice DNA library using the following methods: Denton Davis screen, Nick- translation Southern blotting, hybridization,, digested cloning Cε DNA with EcoRI and ebctrophoresis on the agarose plate. We have got two positive Cεgene clones from 10×106 phages by pIg ε DNA prob...
            本文阐述了以缺口翻译制备的pIge DNA为探针(4.4×10~6cpm/μg DNA),用吸印法从小鼠胎儿基因文库中筛选分离出免疫球蛋白重链C_ε基因克隆。同时,从扩大培养的噬菌体中提取出DNA,并用EcoRI酶消化,0.7%琼脂糖凝胶平板电泳分离,分离的DNA片段转移至硝酸纤维素滤膜上,以pIgs DNA探针杂交,进而确证有两条阳性带,为免疫球蛋白重链C_s基因。电泳法测定其大小分别约为3.5 kb和3.0 kb。也显示出在小鼠胎儿中的未分化型免疫球蛋白重链C_g基因内有一个限制性内切酶Eco RI位点。
文摘来源
         A cDNA library was constructed from plasmid pBK322 and the poly (A)+ RNA extracted from the EC cell line PCC3. E. coli colonies were differentially hybridized in situ with the 32P labeled cDNA of another EC cell line, F9 and with the 32P labeled cDNA of a differentiated cell line, TDM1. Out of 3,000 recombinant clones screened, 25 were found to give a signal only with the cDNA from the uudifferentiated cell line. One of them, designated as MS3 cDNA clone, contains a 250 bp DNA insertion into the PstI ...
            用胚胎癌细胞株(EC)PCC_3,的poly(A)~+RNA及质粒pBR 322在大肠杆菌中建立了一个eDNA文库。用另一株EC细胞F(?)及由EC细胞衍生的分化细胞株滋养层母细胞癌TDM_1的~(32)PcDNA作探针,对PCC_3 eDNA文库作了差别筛选。自3,000个cDNA克隆中筛出25个克隆。其中1个克隆MS,含有长250bp的cDNA。用MS,cDNA作探针,在F(?)细胞中能检出1个占poly(A)~+RNA约0.2—0.4%、长6kb的RNA(MS_3RNA)。用点印迹杂交及Northern印迹杂交分析法,证明MS_3RNA还存在于供试的另2株未分化EC细胞株PCC_3、PSA_1-NG_2中。在供试的全部分化细胞株,即TDM_1、PSA_1-2、3/A/1-D3、C_(17)-S_1-T(284)中,均查不到MS_3RNA。用Southern印迹杂交分析法,证明MS_3基因在小鼠基因组中存在着大量的拷贝,对小鼠胚胎基因文库的筛选表明,MS_3基因有一个中等重复的基因家族,这个家族在小鼠基因组中约有3,000个成员。
文摘来源
         Recent studies on library search and pattern recognition methods which arecommonly used in computer-aided interpretation of mass spectra have beenreviewed. The basic principles of them are briefly discussed. The evaluationof performance of some typical systems have also been given.
            本文综述了计算机辅助质谱解析中常用的谱图匹配及模式识别方法的近期进展。扼要阐述了有关方法的原理,对某些具有代表性的系统作了简单介绍和评价。
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