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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
A library of 363 glycoconjugates and C-nucleosides synthesized by our earlier reported methods were screened for their effect on isolated filarial glutamate cysteine ligase (GCL) and glutathione reductase (GR).
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The library has been tested against Leishmania donovani and Plasmodium falciparum, with activity at the micromolar level.
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Aptamers which specifically recognize targets are selected from random oligonucleotide library using systematic evolution of ligands by exponential enrichment (SELEX).
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Screening of Peptide Inhibitors of TACE from a Phage Display Random 15-Peptide Library by Recombinant TACE Ectodomain
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In the present study, a 15-peptide library was used to identify potential TACE antagonists.
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| | Although immunoglobulin IgE has an imoortant biological function as the mediator of allergic reaction, we know little about the genetics of the Cε gene. For its further study we have screened and isolated the Cε gene from the new born mice DNA library using the following methods: Denton Davis screen, Nick- translation Southern blotting, hybridization,, digested cloning Cε DNA with EcoRI and ebctrophoresis on the agarose plate. We have got two positive Cεgene clones from 10×106 phages by pIg ε DNA prob... | | 本文阐述了以缺口翻译制备的pIge DNA为探针(4.4×10~6cpm/μg DNA),用吸印法从小鼠胎儿基因文库中筛选分离出免疫球蛋白重链C_ε基因克隆。同时,从扩大培养的噬菌体中提取出DNA,并用EcoRI酶消化,0.7%琼脂糖凝胶平板电泳分离,分离的DNA片段转移至硝酸纤维素滤膜上,以pIgs DNA探针杂交,进而确证有两条阳性带,为免疫球蛋白重链C_s基因。电泳法测定其大小分别约为3.5 kb和3.0 kb。也显示出在小鼠胎儿中的未分化型免疫球蛋白重链C_g基因内有一个限制性内切酶Eco RI位点。 | | 文摘来源 | | A cDNA library was constructed from plasmid pBK322 and the poly (A)+ RNA extracted from the EC cell line PCC3. E. coli colonies were differentially hybridized in situ with the 32P labeled cDNA of another EC cell line, F9 and with the 32P labeled cDNA of a differentiated cell line, TDM1. Out of 3,000 recombinant clones screened, 25 were found to give a signal only with the cDNA from the uudifferentiated cell line. One of them, designated as MS3 cDNA clone, contains a 250 bp DNA insertion into the PstI ... | | 用胚胎癌细胞株(EC)PCC_3,的poly(A)~+RNA及质粒pBR 322在大肠杆菌中建立了一个eDNA文库。用另一株EC细胞F(?)及由EC细胞衍生的分化细胞株滋养层母细胞癌TDM_1的~(32)PcDNA作探针,对PCC_3 eDNA文库作了差别筛选。自3,000个cDNA克隆中筛出25个克隆。其中1个克隆MS,含有长250bp的cDNA。用MS,cDNA作探针,在F(?)细胞中能检出1个占poly(A)~+RNA约0.2—0.4%、长6kb的RNA(MS_3RNA)。用点印迹杂交及Northern印迹杂交分析法,证明MS_3RNA还存在于供试的另2株未分化EC细胞株PCC_3、PSA_1-NG_2中。在供试的全部分化细胞株,即TDM_1、PSA_1-2、3/A/1-D3、C_(17)-S_1-T(284)中,均查不到MS_3RNA。用Southern印迹杂交分析法,证明MS_3基因在小鼠基因组中存在着大量的拷贝,对小鼠胚胎基因文库的筛选表明,MS_3基因有一个中等重复的基因家族,这个家族在小鼠基因组中约有3,000个成员。 | | 文摘来源 | | Recent studies on library search and pattern recognition methods which arecommonly used in computer-aided interpretation of mass spectra have beenreviewed. The basic principles of them are briefly discussed. The evaluationof performance of some typical systems have also been given. | | 本文综述了计算机辅助质谱解析中常用的谱图匹配及模式识别方法的近期进展。扼要阐述了有关方法的原理,对某些具有代表性的系统作了简单介绍和评价。 | | 文摘来源 | |   | | << 更多相关文摘 |
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