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|  | | 为了更好的帮助您理解掌握查询词或其译词在地道英语中的实际用法,我们为您准备了出自英文原文的大量英语例句,供您参考。 | |
A library of 363 glycoconjugates and C-nucleosides synthesized by our earlier reported methods were screened for their effect on isolated filarial glutamate cysteine ligase (GCL) and glutathione reductase (GR).
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The library has been tested against Leishmania donovani and Plasmodium falciparum, with activity at the micromolar level.
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Aptamers which specifically recognize targets are selected from random oligonucleotide library using systematic evolution of ligands by exponential enrichment (SELEX).
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Screening of Peptide Inhibitors of TACE from a Phage Display Random 15-Peptide Library by Recombinant TACE Ectodomain
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In the present study, a 15-peptide library was used to identify potential TACE antagonists.
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| | Using a oligonucleotide as probe, we screened a human liver cDNA library with colony hybti-dization. A positive cDNA clonc coding the human epmplement C3 was isolated and named ReC3-N9. By identification with DNA assays, it showed that the clone was situated on the human C3 β-chain, with an inserting fragment about 0.5 kb in length and several points recognized by some restriction endonucleases, namely BamH I, Sca I, Bal I, Sac I and Sma I. Using the ReC3-N9 as a probe, we found some clones which are ... | | 用分子生物学方法从人肝cDNA文库中筛选到一个人补体C3 cDNA克隆,经多种指标鉴定,初步证实该克隆位于人C3β链,用它做探针,筛选到多个与C3β链有同源性的cDNA克隆。同时构建一组质粒表达载体PMS-31b。c和PEX-3 P。将C3β链cDNA片段亚克隆到PEX-3P中,在大肠杆菌表达出融合蛋白。 | | 文摘来源 | | Genomic DNA from a child with severe β-thalassemia from Guangdong Province was isolated and digested to completion using HindⅢ. DNA fragments of 6-9 kb in size were recovered using DE-81 paper and inserted into A Charon 28. A library was constructed through in vitro packaging and transfection of E.coli k802. Three positive clones were obtained from 1.4×105 plaques by screening according to Benton's procedure. Restriction mapping was 'done on DNA of one of the clones and the results were consistant wit... | | 从1例广东籍重型β地贫患儿的外周血白细胞中分离基因组DNA。回收用HindⅢ完全酶解产生的6~9kb长度的DNA片段,与λCharon 28噬菌体DNA插入重组,构建基因文库。用~(32)P标记的βIVSⅢ(0.9kb)DNA片段作为探针,从1.4×10~5噬菌斑中筛出3个带有β地贫基因的阳性克隆,对其中一个阳性重组体DNA进行限制酶谱分析,进一步分离得到带有β地贫基因的3.7kb DNA片段,与噬菌体M13mp19重组,采用斑点杂交法筛选出阳性次级克隆。制备阳性M13mp19单链重组体DNA,用作DNA序列分析。在国内完成了首例β地中海贫血基因的克隆化分离。 | | 文摘来源 | | A cDNA sequence was isolated from cDNA library which was hybridized with artificial human IL-4 probe. The cDNA sequence was inserted into πH3M plasmid and was intruded into COS monkey cells. Human IL-4 biological activity was identified from the culture supernatant by FACS analysis. | | 将PHA活化的人外周血白细胞cDNA文库同人工合成的人IL-4探针杂交,解离了一段cDNA序列,将它插入πH3M质粒并导入COS猿猴细胞。经FACS测定证实细胞的培养上清中具有人IL-4的生物学活性。 | | 文摘来源 | |   | | << 更多相关文摘 |
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