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Paroxymal nocturnal hemoglobinuria ( PNH ) is one of the common hemolytic diseases in this country. The hemolysis is due to a defect of the cell membrane which renders it more susceptible to the lytic action of the complement. Kabakci (1972)used the Cobra Venom Factor(CoF)to activate the complement in the hemolysis test and considered it to be specific in the diagnosis of PNH. But because of the complicated procedures in the preparation of the CoF, this test has not been universally adopted. The method... Paroxymal nocturnal hemoglobinuria ( PNH ) is one of the common hemolytic diseases in this country. The hemolysis is due to a defect of the cell membrane which renders it more susceptible to the lytic action of the complement. Kabakci (1972)used the Cobra Venom Factor(CoF)to activate the complement in the hemolysis test and considered it to be specific in the diagnosis of PNH. But because of the complicated procedures in the preparation of the CoF, this test has not been universally adopted. The method of purification of the CoF was modified in our laboratory by using slab polyacrylamide gel electrophoresis which is a simple method and CoF of purer quality can be obtained. The method of the hemolysis test of Kabakci was also modified and simpified. Only 0.1 ml blood from the ear lobe is taken for the test, compatible serum is used as a source of complement and purified CoF solution is added and result can be read in one hour. The results obtained with this method are in agreement with those using Kabakci method.Since 1978 the CoF hemolysis test was performed in normal individuals, PNH patients and cases of various types of anemia. The percentage of hemolysis in 22 normal individuals was 0-5.6%, of these the value was below 4% in 19 cases, the mean value was 2.4%. The percentage of hemolysis in 21 cases of PNH patiants was 7.0-69.0% with a mean value of 27.9±2.3%. of 8 cases suspicious of PNH the CoF hemolysis was normal in 5 cases and slightly increased in 3 cases, with a mean value of 5.4±0.72%.The result of our studies shows the CoF hemolysis test completely in agreement with the sucrose water test, and is more sensitive than the acidified serum hemolysis test. Therefore, the CoF hemolysis test is considered to be a useful test for the diagnosis of PNH. 作者对Kabakci提出的蛇毒因子溶血试验进行了改进,并利用改进法对正常人、临床诊断为阵发性睡眠性血红蛋白尿症患者,疑似该症以及其它贫血患者做了对比检查。检查结果表明,本试验与糖水试验完全一致,比酸化血清溶血试验敏感,与红细胞乙酰胆碱酯酶活性的降低大多平行。作者认为改进的蛇毒因子溶血试验可做为阵发性睡眠性血红蛋白尿症的有效诊断方法之一。 The essence of Pelargonium geraveolens has been shown to have a cleaning effect of the surface infection in the treatment of carcinoma of cervix. On the basis of this phenomenon, we used 3 different methods (paper disc, pour plate and fuming) to test the suseptibility of different organisms to this drug. The results showed that most of the Gram positive pyogenic organisms, e.g. Staphylococcus aureas, Staphylococ cus albus, Streptococcus hemolyticus, Corynebacterium diphtheriae and Bacillus subtilis, etc. were... The essence of Pelargonium geraveolens has been shown to have a cleaning effect of the surface infection in the treatment of carcinoma of cervix. On the basis of this phenomenon, we used 3 different methods (paper disc, pour plate and fuming) to test the suseptibility of different organisms to this drug. The results showed that most of the Gram positive pyogenic organisms, e.g. Staphylococcus aureas, Staphylococ cus albus, Streptococcus hemolyticus, Corynebacterium diphtheriae and Bacillus subtilis, etc. were highly susceptible, while the Gram negative organisms were not. In light microscope the B. subtilis became pleomorphic, and in electron microscope the cell wall was peeled off. This drug is considered to have some effect on the peptoglycans of the cell wall of the Gram positive organism. 用香竺葵油治疗子宫颈癌时发现有清洁组织表面感染的作用.根据此现象,本室应用三种不同方法(纸片、倾注平板和蒸熏法)检测多种细菌对该药的敏感性.结果表明香竺葵油对革兰氏阳性常见化脓菌,如金黄色葡萄球菌、白色葡萄球菌、乙型链球菌、白喉杆菌及枯草杆菌等有较强杀菌作用,而对革兰氏阴性无作用.通过光镜观察香竺葵油可改变枯草杆菌形态呈现多形性,电镜下可见细菌细胞壁有明显缺损脱落.因此认为该药作用机制在于对革兰氏阳性菌细胞壁的糖肽有作用,从而导致细菌裂解死亡. In vitro antibacterial activities of Home-made cefoperazone against 440 clinical isolates were evaluated by a two-fold broth dilution method and compared with those of German-made cefoprazone. Results showed that the cumulative percentage MIC and MBC of both agents were essentially the same for all strains tested. 83.9% of the strains were inhibited at 8 μg/ml by either agent. Staphylococcus aureus was susceptible to Home-made cefoperazone(MICs≤8μg/ml; MIC90, 4μg/ml). Its actions on different species of... In vitro antibacterial activities of Home-made cefoperazone against 440 clinical isolates were evaluated by a two-fold broth dilution method and compared with those of German-made cefoprazone. Results showed that the cumulative percentage MIC and MBC of both agents were essentially the same for all strains tested. 83.9% of the strains were inhibited at 8 μg/ml by either agent. Staphylococcus aureus was susceptible to Home-made cefoperazone(MICs≤8μg/ml; MIC90, 4μg/ml). Its actions on different species of bacteria of Enterobacteriaceae were remarkable. MIC90 was 0.06 μg/ml for Shigella species, 8μg/ml for Escherichia coli and Proteus species MIC50 s were 0.125 to 0.25μg/ml for Salmonella species, Hafnia species, Enterobacter aerogenes and Klebsiella pneumoniae and MIC90s were 16 to 32 μg/ml. Citrobacter species were less susceptible (MIC50, 4μg/ml; MIC90,64ug/ml). Home-made cefoperazone showed a good activity for Pseudomonas aeruginosa (MIC50, 4 μg/ml; MIC90, 32 μg/ml), and the majority (70%)which was inhibited at or below 8μg/ml. Strains of Streptococcus faecalis, Enterobacter cloacae and Acinetobacter were less susceptible or resistant to this agent. 本文报道国产头孢哌酮对临床分离的440株致病菌的体外抗菌作用研究结果,并与联邦德国进口产品进行比较.结果表明;头孢哌酮国产品与进口品对440株细菌的累积抑菌和杀菌百分率基本一致,8μg.ml时均能抑制83.9%的菌株.对常见的10种(405株)细菌,其MIC_(50)、MIC_(90)和 MIC范围也基本相同.金黄色葡萄球球菌对国产头孢哌酮敏感(MIC_(90)为4μg/yml).国产头孢哌酮对革蓝氏阴性杆菌有明显抗菌活性,对痢疾杆菌、大肠杆菌和变形杆菌,其MIC_(90)≤8μg/m1,对于沙门氏菌、哈夫尼亚菌、产气肠杆菌、肺炎杆菌和绿脓杆菌,其MIC_(50).范围为0.125~4μg/ml,MIC_(90)范围为16~32μg/ml,8μg/ml时的抑菌率为70~89%;对枸橼酸杆菌抗菌活性稍差,MIC_(50)为4μg/ml,MIC_(90)为64μg/ml.本次实验中株数较少的12种(35株)细菌,对其中的粪链球菌、阴沟肠杆菌和不动杆菌大多抗菌活力不强.
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