Methods rpoB、KatG and rpsL gene mutation of mycobacterium tuberculosis was detected with Polymerase Chain reaction-single strand conformation(PCR-SSCP) from 373 clinical isolates,including 148 drug susceptible strains and 225 INH and RFP resistance strains.
⑵The biotype one was resistant to ciprofloxacin(62.8%),levofloxain(43%),sparfloxacin(44.2%). It was susceptible to doxycycline(98.8%),azithromycin(96.5%),josamycin(93.3%).
Most of the ESBLs producing strain were resistant to cephalosporin,the resistant rate of gentamicin and ciprofloxacin were 71.3%-88.5%,all isolates were susceptible to imipenem,the susceptibility rate to cefoxitin,cefoperazone-sulbactam,piperacillin-tazobactam was 63.8%-92.0%.
The result showed the relative amounts of OprD2 were from 0.00% to 3.5% in strains resistant to carbapenems (except a strain which OprD2 was 8.70%); those were from 6.20% to 10.20% in susceptible strains.
In SSCP analysis, 130 of 140(92.9%) strains resistant to lifampin in L-J drug susceptibility test were rpoB-mutants and 67 of 72(93.1%) strains susceptible to lifampin were wild type.
This paper considers an SIRS epidemic model that incorporates constant immigration rate, a general population-size dependent contact rate and proportional transfer rate from the infective class to susceptible class.
An improved susceptible-infected-susceptible (SIS) model in the local-world evolving network model is presented to study the epidemic spreading behavior with time delay, which is added into the infected phase.
Biocides should be introduced into systems susceptible to biocorrosion at the start of their exploitation, otherwise higher concentrations or synergistic compositions have to be used.
A microbial association in the culture medium averaging 106 yeast cells/l and 108 bacterial cells/l is more susceptible to technogenic impacts and seasonal fluctuations.
However, cells subjected to oxidative stress are significantly less susceptible to the reactivating effect as compared to their UV-irradiated or heated counterparts.
Paroxymal nocturnal hemoglobinuria ( PNH ) is one of the common hemolytic diseases in this country. The hemolysis is due to a defect of the cell membrane which renders it more susceptible to the lytic action of the complement. Kabakci (1972)used the Cobra Venom Factor(CoF)to activate the complement in the hemolysis test and considered it to be specific in the diagnosis of PNH. But because of the complicated procedures in the preparation of the CoF, this test has not been universally adopted. The method...
Paroxymal nocturnal hemoglobinuria ( PNH ) is one of the common hemolytic diseases in this country. The hemolysis is due to a defect of the cell membrane which renders it more susceptible to the lytic action of the complement. Kabakci (1972)used the Cobra Venom Factor(CoF)to activate the complement in the hemolysis test and considered it to be specific in the diagnosis of PNH. But because of the complicated procedures in the preparation of the CoF, this test has not been universally adopted. The method of purification of the CoF was modified in our laboratory by using slab polyacrylamide gel electrophoresis which is a simple method and CoF of purer quality can be obtained. The method of the hemolysis test of Kabakci was also modified and simpified. Only 0.1 ml blood from the ear lobe is taken for the test, compatible serum is used as a source of complement and purified CoF solution is added and result can be read in one hour. The results obtained with this method are in agreement with those using Kabakci method.Since 1978 the CoF hemolysis test was performed in normal individuals, PNH patients and cases of various types of anemia. The percentage of hemolysis in 22 normal individuals was 0-5.6%, of these the value was below 4% in 19 cases, the mean value was 2.4%. The percentage of hemolysis in 21 cases of PNH patiants was 7.0-69.0% with a mean value of 27.9±2.3%. of 8 cases suspicious of PNH the CoF hemolysis was normal in 5 cases and slightly increased in 3 cases, with a mean value of 5.4±0.72%.The result of our studies shows the CoF hemolysis test completely in agreement with the sucrose water test, and is more sensitive than the acidified serum hemolysis test. Therefore, the CoF hemolysis test is considered to be a useful test for the diagnosis of PNH.
The essence of Pelargonium geraveolens has been shown to have a cleaning effect of the surface infection in the treatment of carcinoma of cervix. On the basis of this phenomenon, we used 3 different methods (paper disc, pour plate and fuming) to test the suseptibility of different organisms to this drug. The results showed that most of the Gram positive pyogenic organisms, e.g. Staphylococcus aureas, Staphylococ cus albus, Streptococcus hemolyticus, Corynebacterium diphtheriae and Bacillus subtilis, etc. were...
The essence of Pelargonium geraveolens has been shown to have a cleaning effect of the surface infection in the treatment of carcinoma of cervix. On the basis of this phenomenon, we used 3 different methods (paper disc, pour plate and fuming) to test the suseptibility of different organisms to this drug. The results showed that most of the Gram positive pyogenic organisms, e.g. Staphylococcus aureas, Staphylococ cus albus, Streptococcus hemolyticus, Corynebacterium diphtheriae and Bacillus subtilis, etc. were highly susceptible, while the Gram negative organisms were not. In light microscope the B. subtilis became pleomorphic, and in electron microscope the cell wall was peeled off. This drug is considered to have some effect on the peptoglycans of the cell wall of the Gram positive organism.
In vitro antibacterial activities of Home-made cefoperazone against 440 clinical isolates were evaluated by a two-fold broth dilution method and compared with those of German-made cefoprazone. Results showed that the cumulative percentage MIC and MBC of both agents were essentially the same for all strains tested. 83.9% of the strains were inhibited at 8 μg/ml by either agent. Staphylococcus aureus was susceptible to Home-made cefoperazone(MICs≤8μg/ml; MIC90, 4μg/ml). Its actions on different species of...
In vitro antibacterial activities of Home-made cefoperazone against 440 clinical isolates were evaluated by a two-fold broth dilution method and compared with those of German-made cefoprazone. Results showed that the cumulative percentage MIC and MBC of both agents were essentially the same for all strains tested. 83.9% of the strains were inhibited at 8 μg/ml by either agent. Staphylococcus aureus was susceptible to Home-made cefoperazone(MICs≤8μg/ml; MIC90, 4μg/ml). Its actions on different species of bacteria of Enterobacteriaceae were remarkable. MIC90 was 0.06 μg/ml for Shigella species, 8μg/ml for Escherichia coli and Proteus species MIC50 s were 0.125 to 0.25μg/ml for Salmonella species, Hafnia species, Enterobacter aerogenes and Klebsiella pneumoniae and MIC90s were 16 to 32 μg/ml. Citrobacter species were less susceptible (MIC50, 4μg/ml; MIC90,64ug/ml). Home-made cefoperazone showed a good activity for Pseudomonas aeruginosa (MIC50, 4 μg/ml; MIC90, 32 μg/ml), and the majority (70%)which was inhibited at or below 8μg/ml. Strains of Streptococcus faecalis, Enterobacter cloacae and Acinetobacter were less susceptible or resistant to this agent.
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