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vitrification
相关语句
  玻璃化
    Experimental Study of Cryopreservation of Arterial Allografts by Vitrification
    玻璃化法保存异体动脉的实验研究
短句来源
    Methods: Rabbitovarian tissues were cryopreserved by procedure slow-freezing protocols including PROH(A2 group) and DMSO(B2 group) method, and vitrification protocols including DMSO+PROH(C2 group) and DMSO+EG(D2 group) method. After thawing, the frozen-thawed tissue was compared with the fresh tissue in order to analyze their morphologicalchanges.
    方法:采用PROH(A2组)及DMSO(B2组)慢速程序化冷冻和DMSO+PROH(C2组)及DMSO+EG(D2组)玻璃化冷冻方法,冻存家兔卵巢组织,解冻复苏后,以相应的4组新鲜组织为对照(A1-D1组),做HE染色,行组织形态学分析。
短句来源
    Compare them with that of fresh group. Results: In PROH group and DMSO+EG vitrification group,the cpms of small OGC which its morphology was intact after thawed were 2554.67±93.59 and 2510.67±100.03,that of fresh control group was 2507.00±25.94.There were no significant difference in freezing group and fresh group(P>0.05);
    结果PROH组及DMSO+EG玻璃化组复苏后形态正常的小OGC的cpm值分别为2554.67±93.59及2510.67±100.03,新鲜对照组为2507.00±25.94,两冷冻组与新鲜组比较,差异无显著性(P>0.05);
短句来源
    The ICR mice bone marrow nucleated cells(BMNC) were cryopreserved by vitrification. After cryopreservation for 1,5,10,20 and 30 days, the individual samples were separately thawed in 38℃ water bath or by microwave treatment in 300mL 10℃ water under microwave power of 800W, frequency of 2450MHz and thawing duration of 80s, following which the viability of BMNC was assayed.
    对ICR小鼠骨髓有核细胞进行玻璃化深低温保存,在冻存1、5、10、20、30d后,骨髓有核细胞样品分别采用常规的38℃水浴复温和微波复温即在300mL 10℃水中,控制微波功率为 800W,频率2450MHz,复温时间80s。
    Cryopreservation of umbilical cord blood stem cells by vitrification
    脐血干细胞玻璃化冻存技术研究
短句来源
更多       
  玻璃化冷冻
    Methods: Rabbitovarian tissues were cryopreserved by procedure slow-freezing protocols including PROH(A2 group) and DMSO(B2 group) method, and vitrification protocols including DMSO+PROH(C2 group) and DMSO+EG(D2 group) method. After thawing, the frozen-thawed tissue was compared with the fresh tissue in order to analyze their morphologicalchanges.
    方法:采用PROH(A2组)及DMSO(B2组)慢速程序化冷冻和DMSO+PROH(C2组)及DMSO+EG(D2组)玻璃化冷冻方法,冻存家兔卵巢组织,解冻复苏后,以相应的4组新鲜组织为对照(A1-D1组),做HE染色,行组织形态学分析。
短句来源
    Progress in study on vitrification of ovary
    卵巢玻璃化冷冻研究进展
短句来源
    Methods:Rabbit oocytes were verified by using cryoloopwith ethylene glycol(EG)singly or EG combined with dimethyl sulphoxide(DMSO)as cryoprotectants,and cooled by taking oocytes directly into liquid nitrogen or by vitrification machine.
    方法:以冷冻环为载体,单用乙二醇(ethylene glycol,EG)或乙二醇与二甲基亚砜(dimethyl sulphoxide,DMSO)联合作冷冻保护剂,用直投液氮或使用玻璃化冷冻仪高速制冷冷冻家兔卵母细胞;
短句来源
    [Results] 81.9% of the human ES cell clumps were recovered after vitrification, while only 22.8% of cell clumps were recovered after slow freezing. The difference was statistically significant (P< 0.001).
    【结果】玻璃化冷冻组与慢速冷冻组解冻后的细胞复苏率分别为81.9%和22.8%(P<0.001)。
短句来源
    Results: The survival rates of the oocytes after treated by the two vitrification cryopreservation methods had no difference( 80.3 % vs 87.5 %, P> 0.05 ).
    结果:两种玻璃化冷冻方案中,卵母细胞解冻后的存活率差异无显著性(80.3%vs87.5%,P>0.05);
短句来源
更多       
  玻璃化法
    Experimental Study of Cryopreservation of Arterial Allografts by Vitrification
    玻璃化法保存异体动脉的实验研究
短句来源
    Recently, a new method of cryopreservation called vitrification, of which manipulation is simple and less damage to cells and tissues on account of ice-free in the process of cryopreservation.
    新近出现的玻璃化法操作简便,由于在保存过程中没有冰晶形成,因而对细胞和组织所造成的损伤更小。
短句来源
    Up to present, many kinds of cells have been vitrified successfully, and vitrification of tissues is also started.
    现已应用玻璃化法成功地进行了多种细胞的保存,对组织的玻璃化研究也开始起步。
短句来源
    However, vitrification of arterial allografts has not been published in the literature.
    但玻璃化法保存异体动脉的研究在国内外文献中还未见报道。
短句来源
    MethodsThere are five steps as follow: 1) By using the orthogonal design and rapid detection technique of vascular viability (TTC testing), to find out the appropriate conditions of vitrification and freezing of femoral arterial grafts in rabbits.
    现将实验方法分述如下:(1)运用正交设计和血管活性快速检测技术(TTC试验),筛选出适合家兔股动脉玻璃化保存和低温冷冻保存的条件。 (2)应用玻璃化法进行家兔股动脉保存,然后对动脉进行细胞培养,观察培养后细胞的存活情况,并与新鲜动脉和低温冷冻动脉进行比较,以了解玻璃化法保存动脉的细胞活性。
短句来源
更多       
  “vitrification”译为未确定词的双语例句
    Experimental Study on Cryopreservation of Seeding Cells and Preliminary Study on Vitrification of Tissue Engineered Tendons
    肌腱种子细胞深低温保存的实验研究及组织工程化肌腱冻存初步探讨
短句来源
    DATA SOURCES: A computer-based online search of Medline, EBSCO, ScienceDirect Onsite and google scholar based search was undertaken to identify the articles on cells and tissues cryopreservation published in English from February 1980 to May 2006 with the key words of "tissue engineering, engineered tissue, cryopreservation, vitrification, ice-free cryopreservation, vitreous cryopreservation".
    资料来源:应用计算机检索Medline,EBSCO,ScienceDirect Onsite以及google scholar引擎中1980-02/2006-05关于细胞和组织低温保存的文献,检索词“tissue engineering,engineered tissue,cryopreservation,vitrification,ice-free cryopreservation,vitreous cryopreservation”,检索词被分别组合进行检索,限定文献语言种类为English。
短句来源
    Vitrification of mice skin:A method of long-term conservation in vitro
    小鼠皮肤快速冷冻:体外长期保存的方法
短句来源

 

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      vitrification
    The cure degree at gelation turned out to be 0.4539, while the temperature at which vitrification line and gel line transected was found to be 70.2°C.
          
    A thermodynamic derivation of the basic kinetic condition for vitrification-the Frenkel-Kobeko-Reiner equation-is given in a new generalized form.
          
    The results thus obtained underline the substantial differences and formal similarities between vitrification, considered as a diffuse kinetic transition and thermodynamic phase transformations.
          
    It is demonstrated that the structural inhomogeneity of the system slightly increases with a decrease in temperature and vitrification of the melt.
          
    Characterization of the glass-ceramic material prepared upon vitrification of an iron-containing surrogate of high-level wastes
          
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    Rat embryonic cerebral neurocytes(RECN) were cryopreserved by vitrification at-196℃. The samples were separately thawed after storage for 1,10,20 and 40 days,and then cryoprotective solution was diluted and removed. RECN were assayed for their viability and function,and were transplanted for spinal cord injury in rats. The results showed that RECN cryopreserved by vitrification for 40 days retained survival rate of 76.4±8.3%. Compared with the fresh neurocytes,the cell viabiliry and membrane integrity...

    Rat embryonic cerebral neurocytes(RECN) were cryopreserved by vitrification at-196℃. The samples were separately thawed after storage for 1,10,20 and 40 days,and then cryoprotective solution was diluted and removed. RECN were assayed for their viability and function,and were transplanted for spinal cord injury in rats. The results showed that RECN cryopreserved by vitrification for 40 days retained survival rate of 76.4±8.3%. Compared with the fresh neurocytes,the cell viabiliry and membrane integrity of the frozen thawed RECN were decreased,but they still maintained at relatively high level.Synaptic connection was established in neuron culture for 1 week. Cryopreserved RECN were transplanted for spinal cord injury in rats,there was some recovery both in sensation and movement function. No obvious difference was found between vitrification and slow cooling group.

    采用玻璃化冻存技术对大鼠胚胎神经细胞进行深低温保存,在冻存1d、10d、20d和40d后,38℃水浴快速复温,离心洗脱冷冻保护剂,检测胚胎神经细胞的活性与功能,并移植于脊髓损伤大鼠.实验结果表明:玻璃化冻存40d的胚胎神经细胞,存活率为76.4±8.3%,膜完整性为冻存前的69.4±7.8%.细胞活力虽有下降,但仍可维持较高水平.培养1周的部分神经元建立突触联系,移植于脊髓损伤大鼠仍有一定的修复作用

    The relation between cryobiology, cryomedicine and thermophysics is discussed. The injury of cells and tissues during freezing is caused both by ice forming, and by the highly concentrated solution; and this process is closely related to heat and mass transfer. The mechanism of successful cryopreservation is the vitrification (non crystallization) of solutions. The recent hot spots of cryobiology and cryomedicine are discussed; the thermophysics problems concerned are analyzed also.

    本文阐述了低温生物学、低温医学与热物理的关系 ;着重讨论生物体低温保存的问题 .细胞和组织的低温损伤是溶液冻结相变过程所引起的冰晶损伤和高浓度溶液的损伤 ;这个过程和传热传质密切相关 ;而低温保存的机理则是溶液的非晶态化 (玻璃化 ) .本文还讨论了低温生物医学当前的研究热点 ,以及其中的热物理问题

    In this paper we studied the detemperature rate of freezing process and thawing process of vitrification preservation using straw and open pulled straw(OPS).We analyzed the result comparing with differential scanning calorimetry(DSC)curve of vitrification solution.The result is shown that the detemperature rate of freezing process increases from 2 500~4 700 K/min using straw to 11 400~18 400 K/min using OPS and the detemperature rate of thawing process increases from 4 200~6 700 K/min to 12 200~32...

    In this paper we studied the detemperature rate of freezing process and thawing process of vitrification preservation using straw and open pulled straw(OPS).We analyzed the result comparing with differential scanning calorimetry(DSC)curve of vitrification solution.The result is shown that the detemperature rate of freezing process increases from 2 500~4 700 K/min using straw to 11 400~18 400 K/min using OPS and the detemperature rate of thawing process increases from 4 200~6 700 K/min to 12 200~32 300 K/min.In some solution,the devitrification problem during thawing process is more important.The detemperature rate of OPS during thawing process is faster than that during freezing process,which is good for prevent the occurring of devitrification.

    研究了普通麦管和拉伸麦管在玻璃化保存过程中的降温速率和复温速率 ,结合溶液差示扫描量热曲线进行分析。研究表明采用拉伸麦管可将降温速率由普通麦管的 2 50 0~ 4 70 0K/min提高到 1 1 4 0 0~ 1 84 0 0K/min ;将复温速率由普通麦管的 4 2 0 0~ 6 70 0K/min提高到 1 2 2 0 0~ 32 30 0K/min。某些溶液解冻时的反玻璃化问题更需要重视 ,拉伸麦管的复温速率高于降温速率 ,有利于防止反玻璃化产生。

     
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