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silkworm pupa     
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  蚕蛹
     The optimized medium is: 200g/L photato extract,30g/L white sugar,peptone 3g/L,silkworm pupa powder 12.5g/L,KH_2PO_41g/L,MgSO_4 0.5g/L,CuSO_4 0.05g/L,Vitamin B_1100mg/L.
     经优化后的发酵培养基配方为:马铃薯200g/L,绵白糖30g/L,蛋白胨3g/L,蚕蛹粉12.5g/L,磷酸二氢钾1g/L,硫酸镁0.5g/L,硫酸铜0.05g/L,VB1100mg/L。
短句来源
     The degreased silkworm pupa is hydralized with two kinds of microbiological enzyme,the optimum technical conditions are:reaction temperature of neutral protease 3942 is 40 ℃,pH is 7.0,solid liguid ratio is 20∶100,amount of enzyme 2.0%,reaction period 2 h;
     蚕蛹脱脂后,用微生物酶进行双酶水解,确定其最佳工艺条件为:3942中性蛋白酶反应温度为40℃,pH为70,固液比为20∶100,加酶量为20%,反应时间为2h;
短句来源
     Study on Super Critical CO_2 Extraction of The Oil from Silkworm Pupa
     超临界CO_2流体萃取蚕蛹油脂研究
短句来源
     75 kg defatted silkworm pupa powder was hydrolyzed with 6kg crude neutral protease at pH 7-8 and 40-50 C for 10 hours,then separated, decolourized , concentrated and spray-dried to obtain about 30 kg peptones.
     75kg脱脂蚕蛹以6kg中性蛋白酶粗品在pH7—8、40—50℃、水解10小时,随后分离、脱色、浓缩和喷雾干燥,得到约30kg蛋白胨。
短句来源
     Apoptosis of human hepatoma cells SMMC-7721 induced by selenium-abundant amino acids from silkworm pupa
     富硒蚕蛹氨基酸诱导人肝癌细胞SMMC-7721凋亡的研究
短句来源
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  家蚕蛹
     After the silkworm pupa at the compound eye darkened stage was stored in 4℃ for 24h, it could be infected by Ac NPV (Autographa californica Nuclear polyhedrosis Virus).
     家蚕蛹在复眼着色期,经4℃冷藏24小时后,可被Ac NPV(苜蓿银纹夜蛾核多角体病毒)感染。
短句来源
     Rang of Haemolymph pH of treatment was from 5.5 to 7.0 . Changes degree of Haemolymph pH of Japanese silkworm pupa was bigger than Chinese silkworm pupa .
     健康家蚕蛹(对照组)蛹血pH值变化度不大,染病家蚕蛹(处理组)蛹血pH值在5.5-7之间,对蚕蛹来说保持平稳的血液pH值对于保证自身生理活动的正常进行具有重要意义。
短句来源
     The recombi-nant Ac NPV containing HBsAg(Human Hepatitis B Virus Surface Antigen S) gene was also expressed in the silkworm pupa.
     含HBsAg基因(人乙肝表面抗原)的重组Ac NPV亦可感染家蚕蛹,并能正确表达外源基因。
短句来源
     In addition,it was discovered that the silkworm pupa in the early development stage could be induced to "artificial diapause pupa "after it was injected about 5×105 PFU Ac NPV. The pupa lived over 30 days in 25℃. But the development of pupa stopped at the stage of the pre-compound eye darkened.
     此外,还发现化蛹后第2天的家蚕蛹被注射约5×10~5PFU的Ac NPV,可诱导“人工滞育蛹”现象的发生,在25℃经过30天后蛹仍存活,发育停滞在复眼着色前阶段。
短句来源
     LT50 of Chinese silkworm pupa inoculated by same strain was longer than Japanese silkworm pupa,this showed that its resistence to white muscardine was weaker .
     分离自原寄主家蚕的白僵菌对家蚕蛹的致死中时LT50最小,致病力最强。 而同为分离自原寄主家蚕的卵抱白僵菌SP对家蚕蛹的LT50较白僵菌和 B262为大,显示卵抱白僵菌的致病力较球抱白僵菌为弱。
短句来源
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  家蚕蚕蛹
     Methods Ion exchange chromatography,gel filtration,SDS-PAGE and Western-blotting technique were used to identify and partially purify the allergens from the crude silkworm pupa extract.
     方法采用十二烷基磺酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)和Western-blotting,鉴定了家蚕蚕蛹粗浸液中变应原蛋白质分子,并通过阴离子交换和凝胶过滤层析分别对家蚕蚕蛹变应原蛋白质进行初步纯化。
短句来源
  家蚕蛹
     After the silkworm pupa at the compound eye darkened stage was stored in 4℃ for 24h, it could be infected by Ac NPV (Autographa californica Nuclear polyhedrosis Virus).
     家蚕蛹在复眼着色期,经4℃冷藏24小时后,可被Ac NPV(苜蓿银纹夜蛾核多角体病毒)感染。
短句来源
     Rang of Haemolymph pH of treatment was from 5.5 to 7.0 . Changes degree of Haemolymph pH of Japanese silkworm pupa was bigger than Chinese silkworm pupa .
     健康家蚕蛹(对照组)蛹血pH值变化度不大,染病家蚕蛹(处理组)蛹血pH值在5.5-7之间,对蚕蛹来说保持平稳的血液pH值对于保证自身生理活动的正常进行具有重要意义。
短句来源
     The recombi-nant Ac NPV containing HBsAg(Human Hepatitis B Virus Surface Antigen S) gene was also expressed in the silkworm pupa.
     含HBsAg基因(人乙肝表面抗原)的重组Ac NPV亦可感染家蚕蛹,并能正确表达外源基因。
短句来源
     In addition,it was discovered that the silkworm pupa in the early development stage could be induced to "artificial diapause pupa "after it was injected about 5×105 PFU Ac NPV. The pupa lived over 30 days in 25℃. But the development of pupa stopped at the stage of the pre-compound eye darkened.
     此外,还发现化蛹后第2天的家蚕蛹被注射约5×10~5PFU的Ac NPV,可诱导“人工滞育蛹”现象的发生,在25℃经过30天后蛹仍存活,发育停滞在复眼着色前阶段。
短句来源
     LT50 of Chinese silkworm pupa inoculated by same strain was longer than Japanese silkworm pupa,this showed that its resistence to white muscardine was weaker .
     分离自原寄主家蚕的白僵菌对家蚕蛹的致死中时LT50最小,致病力最强。 而同为分离自原寄主家蚕的卵抱白僵菌SP对家蚕蛹的LT50较白僵菌和 B262为大,显示卵抱白僵菌的致病力较球抱白僵菌为弱。
短句来源
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      silkworm pupa
    The effect of baculovirus infection into silkworm pupa particularly on programmed fat body degradation during metamorphosis was investigated.
          
    Approximately 297?μg rPDI was purified from a single silkworm pupa.
          
    Hatched larvae were reared on artificial diets composed of silkworm pupa-powder, dry yeasts, yeast extract, sucrose, peptone, squid liver oil, preservatives and distilled water, but their emergence rate was very low.
          
    Diets composed of silkworm pupa-powder, dry yeasts, sucrose, peptone, squid liver oil, agar and distilled water supported larval development.
          
    (Z)-[11,12d2]-11-Hexadecenoic acid was applied topically to the pheromone gland of silkworm pupa.
          
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    Trichogrammatid wasps,especially Trichogromma dendrolimi are widely usedin China to control the agricultural,horticultural and forestry pests.Eggs ofPhilosamia cynthia ricini,Antheraea pernyi and Corecyra cephalonica have beenused as hosts for mass rearing this parasite.However,the full potential of rea-ring and releasing Trichogramma has always been limited by the number of hosteggs available.Development of an artificial host and diet for this parasite wouldwork greatly.We have studied this problem since 1975.In...

    Trichogrammatid wasps,especially Trichogromma dendrolimi are widely usedin China to control the agricultural,horticultural and forestry pests.Eggs ofPhilosamia cynthia ricini,Antheraea pernyi and Corecyra cephalonica have beenused as hosts for mass rearing this parasite.However,the full potential of rea-ring and releasing Trichogramma has always been limited by the number of hosteggs available.Development of an artificial host and diet for this parasite wouldwork greatly.We have studied this problem since 1975.In our tests,the waspsoviposited viable eggs and developed in artificial wax-vaseline capsules with semi-synthetic culture media of which more than 10 formulations were found to bepromising.However the percentage of emergence was very low.When the eggs ofTrichogramma were dissected from the wax-vaseline artificial eggs and reared invitro on the culture media as“hanging drops”,they could develope normally to ad-ults.The life cycle of Trichogramma reared in vitro was the same as that in theeggs of oak silkworm or eri-silkworm.The percentage of females of fhe first gen-eration adults was 71—100%;and they all oviposited viable eggs.Approximately25—79% of the adults reared in vitro in different media could fully expand theirwings.The best culture medium in our studies was haemolymph plasma from oaksilkworm(or eri-silkworm)pupae(2 parts):milk(2 parts):yolk(1.5 parts):Nei-senheimer′s salt solution(0.8 part).

    三年来的(1975—1978)试验证明,赤眼蜂能在多种培养液制成的人工腊卵上产卵并发育,同时还筛选出十余种体外培育赤眼蜂的培养液配方,可使赤眼蜂完成全世代发育,羽化出蜂,子蜂具生活力。并用优选法选出最优配比(柞蚕蛹血淋巴43.1%,鸡蛋黄34.48%,无机混合盐13.79%,猪血清8.62%及蓖麻蚕蛹血淋巴31.75%,牛奶31.75%,鸡蛋黄23.81%,无机混合盐12.70%)。试验证明用悬滴法培育出赤眼蜂的历期与在自然寄主卵中发育所需的历期相仿,而人造腊卵必须在自裂或被挑破以改善通气条件后始可培育出蜂,但发育历期显著延长。

    This paper is the first to be published in a series of studies on the sto-red product insect community ecology,theegeneral properties of the commun-ity,common species,superior species and the damage of insects to storedgrains and other resources were quantitatively studied based on theodata fr-om systematic and general surveysin seven counties of Sichuan provinoe in1983—1984.The general properties of the stored insect community were revealedaccording to the classified and ecological groupsof the community,i.e.verydifferent...

    This paper is the first to be published in a series of studies on the sto-red product insect community ecology,theegeneral properties of the commun-ity,common species,superior species and the damage of insects to storedgrains and other resources were quantitatively studied based on theodata fr-om systematic and general surveysin seven counties of Sichuan provinoe in1983—1984.The general properties of the stored insect community were revealedaccording to the classified and ecological groupsof the community,i.e.verydifferent components appear regularly in various communities but they actsimilarly on different level of ecologcal function.The constructure of thestored insect community was considerably affected by the bettles and the st-ored mites must not be neglected because of their high density.Those species such as Sitophilus zeamais,Callosobruchus chinensis(onlyin legumes),Tribolium spp.,Oryzaephilus surinamensis,Carpophilns dimi-diatus,Sitotcoga cerealella,Tyrophagus putrescentiae and Lariophagus dist-ingendus are more more common based on the ofteniness index estimationand Sitophilus zeamais,Tribolium spp.and Tyrophagus putrescentiae aremost destructive pests of stored grain based on the superiority index estimation in the community.The serious damage of Sitophilus zeamais,Oryzaephilus surinamensis,Tribolium spp.,Sitotroga cerelella,and Tyrophagus putrescentiae to maize,paddy rice,rice,glutinous rice,wheat,flour and silkworm pupae were qu-antitatively compared based on the preference index estimation The spillageswith rich resources provide good conditions for living and reproducing ofstored product insects and hide a number of stored product insect resourcesfor grain shops,grain—processing plants and feed—processing plants,Sohygiene control must be strengthened.

    本文作为仓虫群落生态的初步研究之一,根据1983—1984两年从四川七市(县)进行定时定点系统调查和普查的资料,对仓虫群落的一般结构特征,仓虫群落中的常见种和优势种以及仓虫对储粮及其它资源的为害进行了定量研究。首先,用分类类群和生态类群两种划分法,揭示了仓虫群落的一般结构特征,即不同仓虫群落虽然组成十分不同,但其中的成员都在不同的生态功能水平上起着相似的作用。仓虫群落的结构在很大程度上受鞘翅目昆虫所左右,而螨类由于密度很大,其影响也不能忽视。其次,用常见度指标定量地比较出所研究仓虫群落中的常见种是玉米象、绿豆象(只在豆类中)、锯谷盗、拟谷盗、脊胸露尾甲、麦蛾、腐食酪螨和米象金小蜂;而玉米象、拟谷盗和腐食酪螨又是发生量大、为害严重的优势种,尤其是玉米象。最后,用偏爱度指标定量地比较出玉米象、锯谷盗、麦蛾、拟谷盗和腐食酪螨等主要害虫(螨)对玉米、稻谷、大米、糯米、小麦、面粉、蚕蛹等资源的偏爱度高,为害严重。资源丰富的地脚粮常为大多数仓虫提供了生存、繁殖的有利条件,为粮店、加工厂等潜伏下大量害虫,应加强清洁卫生防治。

    Results of the laboratory experiments indicated that diet PH of the encapsulated artificial eggs influenced the development, emergence, and the efficacy of reproduction of Trichogramma confusum in the rearings. The diet used composed of 30% holo-tlssues of oak silkworm (Antherstea pernyi) pupae, 14% egg yolk, 26X dry milk solution (1056.) and 30% distilled water. PH 6.7-6.95 of the diet was found to be optimal to the parasite. The parasitization of the provided "host eggs" by 7. confusum was 89-92%;...

    Results of the laboratory experiments indicated that diet PH of the encapsulated artificial eggs influenced the development, emergence, and the efficacy of reproduction of Trichogramma confusum in the rearings. The diet used composed of 30% holo-tlssues of oak silkworm (Antherstea pernyi) pupae, 14% egg yolk, 26X dry milk solution (1056.) and 30% distilled water. PH 6.7-6.95 of the diet was found to be optimal to the parasite. The parasitization of the provided "host eggs" by 7. confusum was 89-92%; the pupation rate of the parasite was 79-80%; and the number of emerged wasps per egg was 26-28. These values were 10%, 25% and 30% higher, respectively, than those for the unadjusted diet with PH 6.4. When the haemolymph of oak silkworm pupae alone was used as the diet, the highest number of Trichogramma eggs per' "host egg" was obtained also at PH 6.7.

    本文报道不同pH值饲料制成人工卵,对拟澳洲赤眼蜂寄生、发育和羽化的影响。用柞蚕蛹血淋巴作卵浆制成人工卵试验的结果:以pH6.7的卵中赤眼蜂产卵最多,每一人工卵中可产蜂卵167.4粒,pH6.45的为139.5粒,pH7.1的为127.5粒。用鸡蛋黄、奶粉、柞蚕蛹匀浆作饲料,制成人工卵繁殖拟澳洲赤眼蜂的结果表明:最适宜的饲料pH值为6.7~6.95,其寄生卵率为89~92%,成蛹率79~80%,每卵出蜂数为26~28头,比用没有调节pH的饲料(pH6.4)繁蜂,分别高10%、25%和30%。

     
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