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osteosarcoma cells
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  骨肉瘤细胞
     Influence of neoadjuvant chemotherapy on expression of Cyclin D1,Bcl-2,PCNA and P-gp in Ⅱ B osteosarcoma cells
     新辅助化疗对ⅡB期骨肉瘤细胞Cyclin D1、Bcl-2、PCNA和P-gp表达的影响
短句来源
     Construction of APE1 siRNA expression vector and its inhibition to APE1 in osteosarcoma cells
     APE1 siRNA表达载体的构建及其对骨肉瘤细胞APE1表达的抑制作用
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     Results The positive expression rate of Bcl-2 and PCNA after chemotherapy (39.1%, 47.5%) was much lower than that of osteosarcoma cells before chemotherapy (73.9%, 87.0%).
     结果化疗后Bcl-2和PCNA在骨肉瘤细胞中的阳性表达率(39.1%、47.5%)明显低于化疗前的阳性表达率(73.9%、87.0%)。
短句来源
     Influence of intracellular expression of human bone morphogenetic protein 2 (hBMP2) antisense RNA on biological activity of osteosarcoma cells OS-9901
     人骨形成蛋白2反义RNA对骨肉瘤细胞OS-9901生物学活性的影响
短句来源
     Methods: Human osteosarcoma cells (HOS-8603) and murine myeloid leukemia cells(JCS) were treated with 1.3μ mol/L mTHPC for 16 hours in vitro, and then exposed to 7.5 mW/cm2 intensity of 580 nm light.
     方法:用含 1.3μ mol/L mTHPC (0.8μ g/ml)的培养液孵育人骨肉瘤细胞系 (HOS-8603)和小鼠髓性白血病细胞系 (命名为 JCS细胞 )细胞 16 h后用波长 580 nm、 7.5 mW/cm2光照射。
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  骨肉瘤细胞的
     The Preparation of Human Osteosarcoma Cells Modified by IL- 2 Gene and the Determination of IL -2 and sIL -2R in Patients with Osteosarcoma
     IL-2基因修饰人骨肉瘤细胞的制备及骨肉瘤患者IL-2和sIL-2R测定
短句来源
     Inhibitory Mechanism of L-MTP-PE Rat Peritoneal Macrophages on UMR106 Osteosarcoma cells
     L-MTP-PE激活大鼠巨噬细胞抑制UMR106骨肉瘤细胞的效应及机制
短句来源
     coli and then the MCP- 1 was extracted and purified. Fifty nude mice were divided into 10 groups. For A1- 4 groups, the 5 mice in each group were locally injected with doses of 1μ g, 10μ g, 100μ g, 1 mg of MCP- 1 at the same time when 4.4× 106 osteosarcoma cells were implanted in vivo.
     将 50只裸鼠分为 10组 :其中 A1~ 4组在接种 4.4× 106个骨肉瘤细胞的同时注射 MCP- 1,剂量分别为 1μ g、 10μ g、 100μ g、 1 mg;
短句来源
     The growth of osteosarcoma cells were suppressed by ASODN (P< 0.05,P< 0.01).
     ASODN明显抑制成骨肉瘤细胞的生长(P<0.05)。
短句来源
     Transfection of PIN1 antisense oligonucleotides inhibited proliferation of human osteosarcoma cells
     PIN1反义基因转染抑制人骨肉瘤细胞的增殖
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  成骨肉瘤细胞
     Cloning and sequencing of CD147 gene encoding region in osteosarcoma cells
     人成骨肉瘤细胞CD147基因编码区的克隆和序列测定
短句来源
     An approach to gene therapy for osteosarcoma:cloning the CD147 gene coding region of human osteosarcoma cells and constructing the eukaryotic antisense RNA expr ession vector
     基因治疗成骨肉瘤的探索:人成骨肉瘤细胞CD147基因编码区克隆和反义RNA表达质粒构建
短句来源
     Significance of Stathmin Gene Overexpression in Osteosarcoma Cells
     Stathmin基因在人成骨肉瘤细胞中的表达及意义
短句来源
     To clone the gene encoding human CD147. Reverse transcriptase-polymerase(RT-PCR) was performed to isolate CD147 cDNA encoding region from osteosarcoma cell line MG63 and verify the presence of CD147 mRNA in osteosarcoma cells.
     克隆人成骨肉瘤细胞CD147基因编码区并进行序列测定。 以人成骨肉瘤细胞系MG63的mRNA为模板,采用RT-PCR方法得到人CD147的编码区cDNA,克隆至载体pcDNA3.1穴+雪中,酶切鉴定后进行序列分析。
短句来源
     Ultrastructure changes of osteosarcoma cells inactivated by alcohol in different concentrations
     不同浓度酒精灭活后的国人成骨肉瘤细胞超微结构的变化
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  “osteosarcoma cells”译为未确定词的双语例句
     Research of TGF-β_1 Gene Expression in FSK88-Treated Cultured Rat osteosarcoma Cells (UMR106) by RT-PCR
     应用RT-PCR方法研究FSK88处理的UMR106细胞TGF-β_1的基因表达
短句来源
     Objective To investigate the effects of simulated weightlessness on the activity of MAPK/ERK kinase 1(MEK1) induced by bone morphogenetic protein-2 (BMP-2) in rat osteosarcoma cells (ROS17/2.8).
     目的观察模拟失重条件下由骨形态发生蛋白-2(BMP-2)诱导的大鼠骨肉瘤成骨样细胞(ROS17/2·8)中丝裂原激活的蛋白激酶/细胞外信号调节激酶的激酶1(MAPK/ERKkinase1,MEK1)活性的变化。
短句来源
     Results:ATRA could significantly inhibit proliferation and induce morphological differentiation of osteosarcoma cells,the value decreased from 0.8405±0.1305 to 0.6147±0.0427(P<0.05).
     结果:经诱导剂处理后,MG-63细胞的生长明显受到抑制,D值从0.8405±0.1305下降到0.6147±0.0427(P<0.05),细胞形态呈良性分化;
短句来源
     Influence of preoperative chemotherapy on expression of Bcl-2, PCNA in osteosarcoma cells and clinical meaning
     术前化疗对ⅡB期骨肉瘤Bcl-2、PCNA表达的影响及临床意义
短句来源
     Conclusion The BMP-2 can induce kinase activity of ERK and expression of c-fos/c-jun mRNA in rat osteosarcoma cells are reduced under simulated weightlessness.
     结论模拟失重降低了ROS17/2.8细胞中BMP-2诱导的ERK的活性,且抑制了c-fos和c-jun的基因表达。
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  osteosarcoma cells
This study is to examine the effect of human recombinant soluble TRAIL (TNF-related apoptosis-inducing ligand) protein inducing apoptosis in MG-63 human osteosarcoma cells.
      
The apoptosis induced by TRAIL in MG-63 human osteosarcoma cells was analyzed with FACS and TUNEL and the apoptotic bodies were observed by transmission electron microscope.
      
Human soluble TRAIL protein can quickly kill MG-63 osteosarcoma cells selectively, and may have potential value for clinical treatment of osteosarcoma.
      
UMR106 osteosarcoma cells were used as a model to evaluate the anticarcinogenic effects of the VOAspi released from the PβPL film.
      
Antisurvivin oligonucleotides promote in vitro cisplatin induction of apoptosis in osteosarcoma cells
      
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The tumor cell cycle of human osteosarcoma cell line OS-732 was studied by autor-adiography. Experimental result showed that the cell cycle time TC was 18. 7 h, mean mitotic index was 37.3%. TS, TM, TG2 and TG1 was 13.1 h, 1 h, 1 h, and 3.5 h respectively. Mean labelling index was 35.96%. Growth index was 51.34%. This result suggested that OS-732 maintains its growth characteristic and is a ideal cell line to study human osteosarcoma experimentally.

本文应用放射自显影技术研究人成骨肉瘤细胞系OS-732的细胞周期。实验结果表明,细胞周期T_c为18.7小时,平均核分裂指数为3.73‰。T_s=13.1小时,T_M=1小时,T_(G1)=3.6小时,平均标记指数为35.96%,生长指数为51.34%。这些结果提示OS-732细胞系保持其生长特性,对人成骨肉瘤的各种实验研究是较好的模型。

Marked hypercalcemia would be caused by ectodermic solid tumor remote from the bone and Without any indication of bone invasion. It suggested that certain humoral factor secreted by the tumor may contribute to the pathogenesis.We partially purified and characterized bone resorption factor (BRF) from human transitional cell carcinoma (TCC) , rat Walker 256 carcinoma and mouse 7,12 dimethyl benz (a) anthracene (DMBA) induced squamous carcinoma. Gel filtration chromatography of tumor extract demonstrates a single...

Marked hypercalcemia would be caused by ectodermic solid tumor remote from the bone and Without any indication of bone invasion. It suggested that certain humoral factor secreted by the tumor may contribute to the pathogenesis.We partially purified and characterized bone resorption factor (BRF) from human transitional cell carcinoma (TCC) , rat Walker 256 carcinoma and mouse 7,12 dimethyl benz (a) anthracene (DMBA) induced squamous carcinoma. Gel filtration chromatography of tumor extract demonstrates a single major peak of bone resorption activity (BRA, 45Ca release from calvarial bone) that elutes with an apparent MW of about 15,000 dalton. The BRA is associated with increased release of immunoreactive PGE 2 and both can be inhibited by indomethacin and boiling. In TCC and DMBA, the BRA were co-eluted with an activity of stimulating aden-ylate cyclase in rat osteosarcoma cells ROS 17/2.8. It appears that these factors are unique BRF, which are different from other known factors that can cause bone resorption.

某些源自外胚层的骨外实体瘤,它们并未浸润至骨组织,但可引起血钙显著增高,提示这些肿瘤可能分泌体液因子作用于骨导致溶骨。我们从人膀胱癌、大鼠乳腺癌(Walker 256)及7.12-Dimethyl Benz[α]anthracene诱发的小鼠鳞癌的提取液中初步分离鉴定了一种溶骨因子。肿瘤提取液经ultrogel层析,发观仅有一溶骨活性峰(~(45)Ca自乳鼠顶骨培养中的释出率),相当于表观分子量15,000道尔顿。此溶骨活性峰与PGE2生成的活性峰相平行,两者均能被Indomethacin及煮沸所抑制。在膀胱癌及鳞癌中,溶骨活性峰还与刺激大鼠成骨肉瘤细胞腺苷酸环化酶的活性相平行。实验结果表明此溶骨因子不同于其它已知能引起溶骨的因子。

Ten cases of human osteosarcoma have been studied by light microscopy, electron micros-copy and energy dispersive X-ray spectrometer (EDX). Ultrastructurally, osteosarcoma cells areclassified into five types: osteoblastic type. chondroblastic type, fibroblastic type, primitivemesenchymal cell and multinucleated giant cell. All the adovetumor cells are originated fromthe multipotential primitiv mesenchymal cell. The characteristic feature of malignant cells: besides common...

Ten cases of human osteosarcoma have been studied by light microscopy, electron micros-copy and energy dispersive X-ray spectrometer (EDX). Ultrastructurally, osteosarcoma cells areclassified into five types: osteoblastic type. chondroblastic type, fibroblastic type, primitivemesenchymal cell and multinucleated giant cell. All the adovetumor cells are originated fromthe multipotential primitiv mesenchymal cell. The characteristic feature of malignant cells: besides common morphologic characteristics,there are often the intranuclear tube-like inclusion bodies and many electron-dense granulesand directionless microfilaments in the cytoplasm. On the intramatrical collagen fibers are depo-sited many hydroxyapatite crystal particles. EDX analysis showed the elemental compositions of ossiform tissue exist in the tumortissue. The formation of hydroxyapatite crystal particles indicated the initial stage of local cal-cification.

对10例人骨肉瘤进行了光镜病理学,电镜超微结构和能谱元素成份的研究。在超微结构上,将骨肉瘤细胞分类为骨母细胞型,软骨母细胞型,纤维母细胞型,原始间叶细胞和多核巨细胞等五型。各型瘤细胞的组织发生均来源于多能性原始间叶细胞。瘤细胞的恶性特点,除具有肿瘤性共同的形态特征之外,核内常见管状包涵体,胞质里有较多散在致密颗粒和无定向排列的微丝,间质胶原纤维中沉积着羟基磷灰石结晶颗粒。能谱分析说明瘤组织内有骨样组织的元素成份,羟基磷灰石结晶颗粒的形成反应了初期局部钙化。

 
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