Methods Mouse fetal ulnas exposed to0.0,2.5,5.0,10.0and20.0μg/ml fluoride were cultured in vitro. The growth and development,morphological changes of the cultured bone were observed and the contents of hydroxyproline(Hyp)and lipid peroxidation(LPO),and the activities of alkaline phosphatase(AKP)and superoxide dis-mutase(SOD)in the bone tissues were determined.
Hyp and LPO contents of the bone tissue were not markedly different from those in the control group(P>0.05),while the activities of SOD of bone tissue were lower(P<0.05)and the activities of AKP were significantly higher(P<0.05)than those of the control group.
Biomimetic strengthening polylactide scaffold materials for bone tissue engineering
The novel composite mainly consists of nano-hydroxyapatite (n-HA), which is the main inorganic content in natural bone tissue for the PLA.
The crystal degree of the n-HA in the composite is low and the crystal size is very small, which is similar to that of natural bone.
The biomimetic three-dimensional porous composite can serve as a kind of excellent scaffold material for bone tissue engineering because of its microstructure and properties.
The effects of hematopoietic stem/progenitor cells (HSPCs) expanded in the two step coculture with human bone marrow mesenchymal stem cells (hMSCs) on the hematopoietic reconstruction of irradiated NOD/SCID mice were studied.