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   recombinant 在 畜牧与动物医学 分类中 的翻译结果: 查询用时:0.186秒
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recombinant
相关语句
  重组
    STUDY ON THE RECOMBINANT DNA VACCINE COEXPRESSING NEWCASTLE DISEASE VIRUS F GENE AND CHICKEN IL-2
    新城疫病毒F基因与鸡IL-2重组DNA疫苗的研究
短句来源
    The Fusion Protein Gene of Newcastle Disease Virus Strain F_(48)E_8:Sequence Analysis and Expression by a Recombinant Fowlpox Virus
    新城疫病毒F48E8株融合蛋白基因和表达该基因的重组鸡痘病毒
短句来源
    PROTECTION OF CHICKENS AGAINST AVIAN INFLUENZA WITH RECOMBINANT FOWLPOX VIRUE EXPRESSING HA-NA, HA-NP OR NP OF AVIAN INFLUENZA VIRUS
    表达禽流感病毒HA-NA、HA-NP及NP基因重组禽痘病毒的构建及其免疫效力的研究
短句来源
    Construction of Two Recombinant Herpesviruses of Turkey rHVT-gB1/EGFP and rHVT-EGFP
    两种重组火鸡疱疹病毒rHVT-gB1/EGFP和rHVT-EGFP的构建
短句来源
    Recombinant Fowlpox Viruses Expressing HA from Subtype H9N2 of Avian Influenza Virus and/or Chicken Type Ⅱ Interferon and Their Protective Efficacies Against Homologous Challenge in Chickens
    表达和共表达H9亚型禽流感病毒血凝素基因和鸡Ⅱ型干扰素基因的重组鸡痘病毒
短句来源
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  重组体
    Construction of recombinant fowlpox virus expressing fusion gene of Newcastle disease virus
    表达新城疫病毒融合蛋白基因禽痘病毒重组体的构建
短句来源
    Cloning of Cattle IL-18, Construction and Expression of Multi-epitope Recombinant of O Type of Foot-and-Mouth Disease Virus (FMDV) and Detection of Immunitical Activity
    牛IL-18基因克隆、O型FMDV多表位重组体的构建、表达及其实验免疫研究
短句来源
    Two recombinant plasmids were constructed. These include the coding regions for the core protein (pC) and for the core, E 1 and E 2 together (pCE 1E 2).
    应用HCVC (pC)和HCVCE1E2 (pCE1E2 )重组体转染真核细胞并且通过肌注免疫BALB/C小鼠 ,对其体液免疫和细胞免疫进行检测。
短句来源
    Based on genetic analysis of an avian influenza virus AIV H5HA gene was cloned and H7HA gene was synthesized. Then DNA recombinant was constructed for the prevention of H5, H7 subtype influenza virus.
    所以本研究构建了针对H5、H7亚型禽流感病毒的DNA重组体,并进行了小鼠免疫试验研究,旨在为最终获得能同时预防多种亚型禽流感病毒的疫苗奠定基础。
短句来源
    These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products. Fusion proteins expressel by the recombinant gene were thus obtaincd.
    本文应用此系统对细小病毒的非结构蛋白基因NSI进行了克隆和表达试验,包括目的基因的制备、载体DNA的提取、基因拼接、噬菌体的体外包装、重组噬菌体蚀斑和溶原菌的抗体探子筛选以及重组体基因产物的检测,最后获得了由重组体基因表达产生的融合蛋白质。
短句来源
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  重组子
    The transposition of positive recombinant named BAC-S was achieved with competent cell DH10BAC.
    用DH10B_(AC)对其进行转位,得到的重组子PBAC-S。
短句来源
    The PIL-6 cDNA fragment was inserted into pGEX-1? T plasmid to construct the expression plasmid pGPIL-6, the recombinant plasmid was digested by BamH I and Pst I to identify whether the PIL-6 cDNA fragment was inserted into the plasmid in correct orientation, the pGPIL-6 was transformed into E.
    用DNA重组技术将已克隆的猪IL—6 cDNA片断插入pGEX—1λT质粒,构建了猪IL-6基因的原核表达质粒pGPIL-6,转化大肠杆菌,以BamH I酶切筛选、Pst I酶切鉴定转化子,获得了含740bp插入克隆基因正确的重组子
短句来源
    NS2 gene was cloned into the multiple cloning sites of prokaryotic expression vector pGEX-6P-l. The recombinant plasmid PGEX-6P-NS2 was constructed and transformed to the competent cell BL21 (DE3) plysS, Positive bacterium strain was induced by IPTG. SDS-PAGE reveals that the NS2 gene was expressed in E.
    将NS2基因插入到原核表达性质粒pGEX-6P-1的EcoR Ⅰ、BamH Ⅰ多克隆位点之间,将重组原核表达质粒pGEX-6P-NS2转化到BL21(DE3)plysS感受态细胞中,获得了表达NS2基因的阳性亚克隆重组子,在含Amp的LB液体培养基中培养,经IPTG诱导表达,用SDS-PAGE分析表达产物。
短句来源
    The TPIL-2 cDNA fragment was inserted into pGEX-4T-l plasmid to construct the expression plasmid pGTPIL-2, the recombinant plasmid was digested by BamH I and EcoR I to identify whether theTPIL-2 cDNA fragment was inserted into the plasmid in correct orientation.
    用DNA重组技术将已克隆的藏猪IL—2 cDNA片断插入pGEX-4T-1质粒,构建了藏猪IL-2基因的原核表达质粒pGTPIL-2,转化大肠杆菌,以BamHⅠ/EcoRⅠ酶切筛选鉴定转化子,获得了465bp基因片断插入正确的重组子
短句来源
    the specific recombinant plasmid was identified with RV-M primer and M13-47 primer by colony PCR.
    以RV-M/M13-47为引物对阳性重组子进行鉴定,菌落-PCR检测证明八个阳性重组子均为阳性。
短句来源
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  “recombinant”译为未确定词的双语例句
    Study on Cloning and Sequence Analysis and Expression of HN Protein Gene of NDV and Immunogenicity of Recombinant Protein
    新城疫病毒HN蛋白基因的克隆、序列分析、表达及其免疫原性研究
短句来源
    Analysis and Expression of the Capsid Protein Gene from the Chinese Early Isolate NJ85 of Rabbit Hemorrhagic Disease Virus and Application of the Recombinant VP60 Protein
    兔出血症病毒中国早期流行毒株NJ85衣壳蛋白基因的分子结构特征及其表达应用
短句来源
    Initiation of Genomic RNA Replication by a Recombinant Classical Swine Fever Virus RNA-dependent RNA Polymerase
    猪瘟病毒RNA依赖的RNA聚合酶起始基因组RNA复制的分子机制研究
短句来源
    Characteristics and Interactions of Recombinant Proteins of Chicken Interferons and Interferon Receptors
    鸡干扰素与干扰素受体重组蛋白的生物学特性及其相互作用
短句来源
    Vaccination Against Taenia Solium Cysticercosis in Pigs Using Recombinant Oncosphere Antigen TSOL18
    猪带绦虫六钩蚴TSOL18重组基因工程疫苗的研制
短句来源
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  recombinant
Screening of Peptide Inhibitors of TACE from a Phage Display Random 15-Peptide Library by Recombinant TACE Ectodomain
      
After Ni2+-NTA resin affinity chromatography, the purity of the recombinant T800/T1300 protein was more than 90%.
      
The recombinant plasmid containing mutant E83V pPIC3.5K-lip-E83V was expressed in Pichia pastoris GS115.
      
A recombinant strain of Salmonella choleraesuis C500, containing a eukaryotic expression plasmid pBO1 with the immune-dominant epitope of foot-and-mouth disease virus, was constructed.
      
Specific immune response to this recombinant strain was evaluated by oral administration of the recombinant live bacteria pBO1/S.
      
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Experiments of cloning and expressing sequences coding for a nonstructural protein gene NSI from the long leftward open reading frame of the autonomous parvovirus FPV by using bacteriophage λgtll were conducted.These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products.Fusion proteins expressel by the recombinant gene were thus...

Experiments of cloning and expressing sequences coding for a nonstructural protein gene NSI from the long leftward open reading frame of the autonomous parvovirus FPV by using bacteriophage λgtll were conducted.These experiments composed of preparing the target gene DNA, extracting the λgtll DNA, Iigating the target gene DNA with λgtll DNA, in vitro packaging, screening the phage plagues and lysogens, detecting the recombinant gene products.Fusion proteins expressel by the recombinant gene were thus obtaincd.These fusion proteins were specifically bound by both antisera raised against one of the fusion proteins and antisera from a canine parvovirus(CPV) in fected dog.The results obtained showed that the bacteriophage λgtll is a good vector for gene manipulation and its extending use will open vast vistas in serodiagnosis and vaccine prevention.

大肠杆菌噬菌体λgt11是在原有基础上经不断改进而组建成的一个新的基因载体,通过一系列检测方法的建立,已成为一个完整的基因操作系统。本文应用此系统对细小病毒的非结构蛋白基因NSI进行了克隆和表达试验,包括目的基因的制备、载体DNA的提取、基因拼接、噬菌体的体外包装、重组噬菌体蚀斑和溶原菌的抗体探子筛选以及重组体基因产物的检测,最后获得了由重组体基因表达产生的融合蛋白质。此蛋白质能被兔源抗NSI抗体和自然感染细小病毒的犬血清特()性地结合。试验结果表明,λgt11是基因操作的一个有效的载体系统,它在疫病的血清学诊断和疫病预防的研究上具有广阔的应用前景。

We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre...

We have successfully cultured three cell lines BHK-13, BHK-21 and CHO-KI with MC-1 type microcarrier made in our Academy. When the microcarrier was in concentration of 5mg/ml, the cell density was about 30×10~4 cells/ml, all of them could proliferate to 1×10~6 cells/ml after 3-day suspension culture. At this time, inoculated VSV diluted 10 or 10~2 times, incubated at 37℃ for 20-24hs, the viral titer in these media were about 6 Log TCID_(50) or much higher. The yield of CHO-KI cell was the highest. Its titre was 7-8 Log TCID_(50). The titres of them were similar to those in stationary culture and also not a little bit inferior to those proliferated with chick embryo. Now we have already used it in our IFN research. These experiments also showed that the cells could be released from microcarriers with trypsincitrate solution and more rapid stirring speed. So we are sure that this microcarrier suspension culture system must be suitable to produce various vaccines and IFNs with CHO cells transformed by recombinant IFN genes.

我们用我院制备的MC-1型微载体成功地培养了BHK-13,BHK-21,和CHO-KI三株细胞。当微载体浓度为5mg/ml,细胞接种量为30×10~4细胞/ml左右时,一般在三天都可达到1×10~6细胞/ml。此时接种10~(-1)或10~(-2)的VSV,在37℃培养20-24小时,病毒产量可达到6LogTCTD_(50)/ml以上,其中以CHO-KI细胞的产量最高,它们与静止培养的细胞产量基本一致,也不亚于鸡胚细胞增殖的产量。目前用该系统增殖的VSV已用于IFN的研究工作中。实验的结果也表明,用该系统增殖其它病毒和制备疫苗,以及大量培养已引入重组IFN基因的CHO工程细胞也将是可行的。

A cDNA library, constructed from raRNA isolated from adult Schistosoma mansoni was screened with a variety of antisera. Positive clones were recognized by either chronic human infection serum(IHS), chronic mouse infection serum ( IMS ) , rabbit anti-soluble egg antigen serum ( RαSEA ) , rabbit anti-S. japonicnm serum ( RαSj ) , rabbit anti-uninfected Biomphalaria glabrata serum ( RαBg ) or Bovine anti-Fasciola hepatica serum ( BαFh ) . One recombinant clone E-b was studied in detail. The foreign schisto...

A cDNA library, constructed from raRNA isolated from adult Schistosoma mansoni was screened with a variety of antisera. Positive clones were recognized by either chronic human infection serum(IHS), chronic mouse infection serum ( IMS ) , rabbit anti-soluble egg antigen serum ( RαSEA ) , rabbit anti-S. japonicnm serum ( RαSj ) , rabbit anti-uninfected Biomphalaria glabrata serum ( RαBg ) or Bovine anti-Fasciola hepatica serum ( BαFh ) . One recombinant clone E-b was studied in detail. The foreign schisto gene of this clone was proved to be a single copy gene which could be hybridized with fragments from the adult worm genomic DNA. A fusion protein of 145 KD was expressed successfully in the lysogenized host cells, E. coli MC4100 and proved highly reactive with SEA by enzyme immunotransfer blot ( EITB ) . A Sequence analysis of the gene coding for this schistosome protein was accomplished by dideoxy nucleotide chain termination method and revealed that this gene consisted of 855 base pairs.A deduced amino acid sequence from an open reading frame showed that the polypeptide contained 239 residues in which there was a unique strong basic polypeptide fragment comprising 7 repeats of 'Arg-Gly' forming a quite hydro-philic antigenic determinant.

本文报导以曼氏血吸虫(Schistosoma mansoni)成虫mRNA为模板,通过逆转录酶促法合成cDNA。将虫源cDNA插入载体λgtll噬菌体基团组中编码β-半乳糖苷酶的LacZ基因区形成重组DNA,然后导入受体细胞E.coli KM392pmc9(lon~+)建立基因库,并从中筛选出若干株血吸虫抗原基因克隆。一株被兔抗虫卵可溶性抗原高免血清检获的基因克隆,其虫源基因片段经分子杂交证明系单拷贝基因。重组基因在溶原菌株中所表达的融合蛋白通过免疫印迹转移试验(EITB)证明与相关抗体具有很强的结合能力。DNA序列分析揭示该基因编码的多肽系由239个残基组成,其分子量理论推导值为28.7KD,化学性质呈强硷性和强亲水性。引人注意的是此多肽涟中含有一个由7个“精氨酸—甘氨酸”重复组成的结构独特片段。

 
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