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micro bubble
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  微泡
    Objective:The study is to investigate the bio-effects of VEC-304、SMMC-7721 cell line which were treated with low -frequency ultrasound combined with micro bubble agent .
    目的:本课题研究低频超声(low-frequency ultrasound)联合微泡剂(micro bubble agent)对血管内皮细胞株(EVC-304)和肝癌细胞株(SMMC-7721)的损伤和诱导凋亡作用,初步探讨其对两种细胞生物学效应,为低频超声联合微泡剂治疗肿瘤提供实验依据。
短句来源
    Methods:EVC-304 and SMMC-7721 cells are all divided in four groups : Group A: control ; Group B: pure micro bubble agent(made up of SB, Witamin C and HES); Group C: pure ultrasound;
    方法:血管内皮细胞株(EVC-304)和肝癌细胞株(SMMC-7721)均分为四组:A组:空白对照组,B组:单纯微泡剂组(由碳酸氢钠,维生素C和HES代血浆按一定比例配制成),C组:单纯低频超声组,D组:低频超声联合微泡剂组。
短句来源
    The Bio-effects of Low-frequency Ultrasound Combined with Micro Bubble Agent on VEC-304 Cell Line Land SMMC-7721 Cell Line in Vitro
    低频超声联合微泡剂对血管内皮细胞和肝癌细胞生物学效应的体外实验
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  micro bubble
Window of type I micro bubble formation is indicated with onset at 52 seconds and off set at 58 seconds.
      
The periodic micro-bubble agitation in the static hybridization solution could effectively mix the molecular beacon and the beacon complements.
      
The hybridization assay without the micro-bubble actuation revealed that the fluorescence increased fast at the beginning and slowed down after that.
      
The fabricated micro-bubble actuator array was tested in the hybridization buffer.
      
The fabrication of the micro-bubble actuator array followed the conventional MEMS technology.
      
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Objective To explore the role of ultrasound radiation on the permeability of HepG2 cell with/without the intervention of ultrasound contrast SonoVue. Methods HepG2 cell suspension infused with Fluorescent dextran molecules (FD500) was exposed to CW Doppler ultrasound, 1.9 MHz, Tis 0.8, gain 80%,Ⅰ SATA 80.0 mW/cm 2, with/without SonoVue infused. Flow cytometry was used to detect the fluorescent rate. Results The uptake rate of fluorescent cells in control groups (pseudo-US exposure group and only with...

Objective To explore the role of ultrasound radiation on the permeability of HepG2 cell with/without the intervention of ultrasound contrast SonoVue. Methods HepG2 cell suspension infused with Fluorescent dextran molecules (FD500) was exposed to CW Doppler ultrasound, 1.9 MHz, Tis 0.8, gain 80%,Ⅰ SATA 80.0 mW/cm 2, with/without SonoVue infused. Flow cytometry was used to detect the fluorescent rate. Results The uptake rate of fluorescent cells in control groups (pseudo-US exposure group and only with micro-bubble contrast agent group) were (3.06±1.57)% and ( 1.4 ±0.04)% respectively. Significant uptake increases were found in the US exposure group ([23.72±14.4]%) and US with micro-bubble contrast group ([54.78±43.6]%). Conclusion Diagnostic ultrasound destruction of contrast-agent microbubbles can enhance the permeability of HepG2 cell membrane, which contributes to the delivery of large molecules. It maybe become a promising biomolecular therapeutic method on oncology.

目的 探讨超声辐照对人肝癌细胞株HepG2 细胞通透性的影响以及声学造影剂声诺维(SonoVue)的增强作用。方法 用连续多普勒超声辐照人肝癌细胞株HepG2,超声发射频率1.9 MHz,软组织热指数(TIS)为0.8,增益设定为80%,输出声强(ⅠSATA)为80.0 mW/cm2,辐照时间均持续60 s。照射时添加或不添加超声造影剂声诺维(SonoVue)。用流式细胞仪检测细胞荧光染色阳性的阳性率。结果 荧光染色阳性细胞的百分率:无超声辐照组或单纯添加SonoVue组分别为(3.06±1.57)%和(1.4±0.04)%,经超声辐照后为(23.72±14.4)%,添加SonoVue辐照后显著增加至(54.78±43.6)%(均P<0.05)。结论 超声辐照能改变HepG2细胞膜通透性,添加超声造影剂后能显著增强这种作用,有希望成为肿瘤分子生物学治疗的介导手段。

Objective To explore the clinical and biological effects induced with low power ultrasound radiating CO_2 micro-bubble into the vessel. Methods Through peripheral vein injected CO_2 micro-bubble agent, tumor was radiated locally with low power ultrasound (0.5 W) on skin surface 2-3 times weekly, 3-6 times for every patient in total of 83 cases with malignance including those in wall of thorax and abdomen or in cavity of antinode. Results The cases got regression above 50% in 13 cases...

Objective To explore the clinical and biological effects induced with low power ultrasound radiating CO_2 micro-bubble into the vessel. Methods Through peripheral vein injected CO_2 micro-bubble agent, tumor was radiated locally with low power ultrasound (0.5 W) on skin surface 2-3 times weekly, 3-6 times for every patient in total of 83 cases with malignance including those in wall of thorax and abdomen or in cavity of antinode. Results The cases got regression above 50% in 13 cases (26%), steady in 27 cases (54%), progression in 4 cases (8%), improvement in the score of quality of life in 28 cases (56%); post-treatment 4 cases (8%) emerge hepatic function in elevated, which were cases that associated with chemical therapy; amelioration of cancer ache in 35 cases (70%). The marked damage was not found after treated in those patients. Serum liver enzymes ALT and AST were not found abnormal heightening change after local treatment on hepatic malignance. Conclusion It will become a new method without damage for treating malignant tumor with low power ultrasound radiating CO_2 micro-bubble into the vessel.

目的探讨低功率超声辐射血管内微泡剂对恶性肿瘤血管的临床生物疗效。方法83例恶性肿瘤患者,外周静脉注入CO2微泡剂,以低功率低频率超声(0.5W)经皮辐射肿瘤局部,每周2~3次,每例病人治疗3~6次。结果超声组50例疗效治疗后病变缩小达50%以上,呈部分缓解为13例,有效率占总数的26%;稳定为27例,占总数的54%;进展为4例,占总数的8%;生活质量评分改善为28例,占总数的56%;治疗后出现肝功能等指标升高的4例,占总数的8%,均为与化疗联合治疗者;癌痛不适改善为35例,占总数的70%;治疗后局部一般未发现明显损伤;肝脏肿瘤局部治疗后,肝酶ALT、AST未见明显升高。结论低功率超声辐射血管内微泡剂可以成为局部治疗恶性肿瘤的一种无创的新方法。

Objective To evaluate by microbubble ultrasound the value of the waterclyster diagnosis of large bowel polyps.Methods After injecting 1 000~1 500 ml of vibrated microbubble normal saline into dual-cavitary air sac pipe through the rectum,the retrograde colonal by ultrasound scan were hroceeded.Results We had checked 136 patients with micro bubble ultrasound waterclyster and discovered 108 cases of physiol focus and 206 symptoms ;among them,there are 76 cases (70%) of physiol cancer and 139 symptoms (67%);...

Objective To evaluate by microbubble ultrasound the value of the waterclyster diagnosis of large bowel polyps.Methods After injecting 1 000~1 500 ml of vibrated microbubble normal saline into dual-cavitary air sac pipe through the rectum,the retrograde colonal by ultrasound scan were hroceeded.Results We had checked 136 patients with micro bubble ultrasound waterclyster and discovered 108 cases of physiol focus and 206 symptoms ;among them,there are 76 cases (70%) of physiol cancer and 139 symptoms (67%); 6 cases (5%) of ulcer and 19 symptoms (9%);26 cases (24%) of polyps and 48 symptoms(23%) .The detective rate of 48 focus for less than 1.5 cm was 63%,for 1.6 cm~2.5 cm 96% and for beyond 3 cm 100%,the smallest and largest polyp is 0.8 cm×0.9 cm and 4.4 cm×4.3 cm.All cases were verified by video lower gi scopes and pathology or operation.Conclusions This way is easy,safe and noncontrain-dication.The checking-out rate is high and worthy of applying.

目的评估微气水灌肠超声在大肠息肉诊断中的应用价值。方法经置于肛肠内的双腔气囊管注入振荡后产生肉眼能观察到的微气泡的生理盐水1000~1500ml后,运用超声逆向结肠施行扫描检查。结果对136例患者采用微气水灌肠超声检查,检出结肠包块108例,检出病灶206个,其中结肠癌76例(70%),病灶139个(67%),溃疡6例(5%),病灶19个(9%),息肉26例(24%),病灶48个(23%),大肠息肉检出率<1.5cm检出率63%、1.6cm~2.5cm96%,3cm以上100%,检出最小息肉0.8cm×0.9cm,最大息肉4.4cm×4.3cm。全部病例均经纤维结肠镜活检病理诊断或手术证实。结论本方法简便、安全、无禁忌症,检出率高,值得应用。

 
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