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organic extracts
相关语句
  有机提取物
    The Study on the Effects of Organic Extracts From Source Water and Drinking Water on DNA
    水中有机提取物对V79细胞DNA损伤的研究
短句来源
    The Effect of Organic Extracts of Cooking Oil Fume Particulates on Cell Circles of Type Pulmanory Cells in Rats
    烹调油烟颗粒有机提取物对大鼠肺Ⅱ型细胞周期的影响
短句来源
    The results showed that dust storm and normal ambient air PM2.5 and their organic extracts had marked genetic toxicity on lymphocytes. With the increase of treatment concentrations the micronuclei frequency (MNF) increased and the nuclear division index (NDI) values declined in a dose-response manner(p<0.01). Although the inorganic extracts showed dose-response manner but there was no marked difference among the treatments(p>0.05).
    结果表明本试验条件下,沙尘暴和正常天气PM2.5及其有机提取物均使淋巴细胞微核率(micronucleifrequency,MNF)显著增高,核分裂指数(nucleardivisionindex,NDI)显著下降(p<0.01),且存在剂量-效应关系,而无机提取物虽有剂量-效应关系,但各处理浓度之间及其与对照相比差异均不显著(p>0.05);
短句来源
    In the same concentrations, the treatments of dust storm samples and normal samples were not significant different (p>0.05) except the treatments of normal ambient air PM2.5 from Baotou city and its organic extracts were significant higher than those of dust storm(p<0.01).
    相同处理浓度下,除工业城市包头正常天气的PM2.5及其有机提取物处理的微核率显著高于沙尘(p<0.01)外,沙尘暴和正常天气样品处理结果无显著差异(p>0.05);
短句来源
    The treatments of normal ambient air PM2.5 from Baotou city and its organic extracts were significant higher than those of Wuwei city, but the treatments of dust storm PM2.5 and its extracts were not significant different between the cities.
    城市之间相比,只有正常天气PM2.5及其有机提取物处理的微核率包头显著高于武威(p<0.01),而沙尘暴PM2.5及其提取物的所有处理的微核率无城市间差异(p>0.05)。
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  organic extracts
The therapeutic effect of organic extracts of Santolina oblongifolia in adjuvant-carrageenan-induced inflammation (ACII) in Wistar rats was investigated.
      
Inhibition of [3H] fumazenil binding by organic extracts derived from the cerebral cortices of HE rats did not increase with progression of encephalopathy.
      
Alkaline organic extracts of proteolytically digested homogenates of cerebral cortices from HE rats were more effective than corresponding extracts from controls at inhibiting the binding of [3H]flumazenil.
      
Cationic complexes of Eu(III) and Sr(II) with diphosphine dioxides in organic extracts
      
Crude organic extracts of whole tunic and internal tissues contained vanadium metabolites (225 and 750 ppm dry mass, respectively) and were palatable to T.
      
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The rhIFN-p, contained in the suspension of disrupted engineered bacteria , was purified paratially by saturated ammonium sulphate or organic extract-acid precipitation method , then it was purified by chromatography of CPG-PEG in HPLC ,5ephacryl S-200 and Rotofor-IEF. The results showed that CPIE-PEC and S-200 had less effect on activity of rhIFN-β(i. e , high recovery rate , separately 93. 36% and 80. 6% ) ,but purification efficiency was lower than that of Rotofor-IEF, rhIFN-β could be partially...

The rhIFN-p, contained in the suspension of disrupted engineered bacteria , was purified paratially by saturated ammonium sulphate or organic extract-acid precipitation method , then it was purified by chromatography of CPG-PEG in HPLC ,5ephacryl S-200 and Rotofor-IEF. The results showed that CPIE-PEC and S-200 had less effect on activity of rhIFN-β(i. e , high recovery rate , separately 93. 36% and 80. 6% ) ,but purification efficiency was lower than that of Rotofor-IEF, rhIFN-β could be partially purified by Rotofor-IFiF by 3l. 75 times with a recovery rate of 56. 25 % and the specific activity of 1. 02 X 107U/ml protein.

人β型干扰素工程菌经发酵培养、裂解后,用硫酸铵分级分离及有机溶剂抽提-酸沉淀两种方法对粗制品进行部分纯化,然后用疏水层析、凝胶过滤和旋转等电聚焦电泳对部分纯化品再纯化,结果显示:部分纯化的基因工程人β型干扰素经疏水层析、凝胶过滤后,有较高的回收率(前者为93.36%,后者为80.6%);经旋转等电聚焦电泳,一步纯化31.75倍,回收率为56.25%,比活性达1.02×10~7U/mg蛋白。

In this study,rat liver unscheduled DNA synthesis(UDS) assay was used to detect the DNA damage induced by the five subfractionated groups of organic extract of airborne particles in Shanghai,so as to identify the genetic toxicity of each group.The results showed that all of the five groups could induce some UDS responses in primary rat liver cells.The group of PAHs had the strongest DNA damage ability.Meanwhile,the more variation of UDS level were observed in the results of summer samples.It suggested...

In this study,rat liver unscheduled DNA synthesis(UDS) assay was used to detect the DNA damage induced by the five subfractionated groups of organic extract of airborne particles in Shanghai,so as to identify the genetic toxicity of each group.The results showed that all of the five groups could induce some UDS responses in primary rat liver cells.The group of PAHs had the strongest DNA damage ability.Meanwhile,the more variation of UDS level were observed in the results of summer samples.It suggested that many genotoxic substances,besides PAHs,presented in airborne particles which should be further investigated.

为探讨大气颗粒物不同组分对大鼠肝细胞DNA合成的影响,采用大鼠肝细胞的程序外DNA合成(UDS)试验研究上海市大气颗粒物不同有机组分对大鼠肝细胞DNA的损伤作用,以确定不同性质的有机成分的遗传毒性。结果表明:各组分均具有不同程度的DNA损伤作用,以多环芳烃组最明显,夏季样品结果变异较大。说明上海市大气颗粒物中遗传毒性物质的种类较多,除多环芳烃外还有许多其它成分值得重视。

Abstract Objective To study the mutagenic activity of the organic extract and its five fractions of the dust collected from an aluminum electrolytic plant in so as to find out the genetic toxicants with stronger mutagenic activity. Methods The dust derived from aluminum electrolytic plant was collected on a fiberglass filter within the scope of the operator's breathing air. Four samples were equidistantly placed in the electrolytic plant. The sampling time lasted for 2 days. Before and...

Abstract Objective To study the mutagenic activity of the organic extract and its five fractions of the dust collected from an aluminum electrolytic plant in so as to find out the genetic toxicants with stronger mutagenic activity. Methods The dust derived from aluminum electrolytic plant was collected on a fiberglass filter within the scope of the operator's breathing air. Four samples were equidistantly placed in the electrolytic plant. The sampling time lasted for 2 days. Before and after each sampling period, the filter was conditioned at constant relative humidity (50%±2%) and temperature (22℃±2℃) for 24 h and weighted to obtain the weight of suspended dust. The dust laden filter was placed in Soxhlet extractor and the organic material was extracted for 8 h with 250 ml of spectrograde benzene. The benzene solution was filtered, concentrated to 10 ml and then lyophilized to have the dust organic extract. The dust organic extract was fractionated with a modified method of Wynder and Hoffmann into three fractions: organic acid, organic alkali and neutral. The neutral part was divided into aliphatic hydrocarbon, polycyclic aromatic hydrocarbon and polar compounds. The dust organic extract and its five fractions were dissolved in DMSO, and stored in the dark at 4℃. The mutagenic activities of these fractions were detected with two Salmonella typhimurium strains (TA98 and TA100). Both of the two strains bring R factor, and very sensitive to mutagenic activity of environmental pollutant. Based on the results of four step method, they accorded with the experimental standard. A 9000g liver supernatant S9 was prepared from Aroclor 1254 induced male Wistar rats. In the tests of metabolic activation, 50 μl of S9 was used in a plate. Ames test was performed essentially according to the procedure described by Maron and Ames. Dust organic extract and its five fractions were dissolved in DMSO. Three doses of the test were 200 μg/plate, 400 μg/plate and 800 μg/plate, respectively. Meanwhile, blank and positive controls were used, each treatment in triplicate. All of the tests were repeated 3 times. Results Mutagenic activities of the dust organic extract and its five fractions were different from one another although within the concentration range of the test, the dust organic extract and its five fractions all increased in revertants for TA98 and TA100. After addition of S9, revertants increased slightly. Mutagenic activity of the dust organic extract was very strong for TA98 and TA100, and after adding S9, it was higher than that without S9. All polycyclic aromatic hydrocarbon showed a mutagenic activity for TA98 and TA100, with and without S9. All polar compounds, detected for TA98, with and without S9, showed mutagenic activities, but they could result in mutagenic activity for TA100 at the concentrations of 400 800 μg/plate only. Organic acid showed mutagenic activity only at the concentrations of 400 800 μg/plate for TA98 and TA100, with and without S9. In the same conditions, no mutagenic activities were detected in the other two fractions, namely organic alkali and aliphatic hydrocarbon, either for TA98 or TA100, with and without S9. Conclusions The dust organic extract derived from an aluminum electrolytic plant and its three fractions, namely organic acid, polycyclic aromatic hydrocarbon and polar compounds, showed mutagenic activities. According to the results of this study, protective measures should be taken to abate the dust in aluminum plants, control carcinogens and mutagens, and to protect the environment and human health.

MutagenicityinorganicextractsofthedustderivedfromanaluminumelectrolyticplantforSalmonelatyphimuriumWangYumei王毓美,JiaJingfen贾敬芬...

 
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