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The mode and ability of Fusobacterium nucleatum to coaggregate with other oral bacteria and their coaggregation inhibition are investigated in the present study. Method: the techniques of visual assay, spectrophotometric assay and transmission electron microscopy were applied to investigate this phenomenon. Results: It was proven that coaggregation occurred between specific partners. Fusobacterium. nucleatum could coaggregate with Porphyromonas gingivalis, Streptococcus. sanguis and Streptococcus mutans.... The mode and ability of Fusobacterium nucleatum to coaggregate with other oral bacteria and their coaggregation inhibition are investigated in the present study. Method: the techniques of visual assay, spectrophotometric assay and transmission electron microscopy were applied to investigate this phenomenon. Results: It was proven that coaggregation occurred between specific partners. Fusobacterium. nucleatum could coaggregate with Porphyromonas gingivalis, Streptococcus. sanguis and Streptococcus mutans. Coaggregation bridge was also found when F. nucleatum, P.gingivalis and S. sanguis were mixed. The ability of F. nucleatum to coaggregate with both early and late colonizers suggested that it could serve as adherent bridge between these two groups of bacteria. When pretreated at 85℃ for 30 min, P.gingivalis, S.sanguis and S.mutans still showed nearly unchanged ability to coaggregate with unpretreated F. nucleatum, while F. nucleatum under the same pretreatment lost its ability to coaggregate with the rest of bactera which had not ben heated. Lactose and L-rhamnose could inhibit coaggregations above mentioned to a different degree. Conclusions: Coaggregation exits in the dental plaque ecosystem and coaggregation inhibition occurs under some pretreatment which offers theory of ecosystemetic prevention and therapy of periodontitis. 在厌氧菌检验中,培养器材的选择是厌氧菌分离培养成败的关键之一。本室采用简易厌氧培养皿和厌氧罐两种不同器材进行对比试验,报告如下。1材料与方法11器材简易厌氧培养皿为两个磨砂面边缘的玻璃平皿,高2cm,直径85cm;厌氧罐选用高17cm、直径14c... Objective To find out an ideal method used in genotypic identification of Streptococcus mutans by arbitrarily primed polymerase chain reaction finger printing (AP PCR). Method The Streptococcus mutans standard strains were used with different polymerase chain reaction (PCR) conditions on general primer (5′ TGCCGAGCTG 3′) to select an ideal result. Result An ideal PCR reaction was found:94 ℃ 30 s,36 ℃ 30 s,72 ℃ 1 min,45 cycles. Conclusion AP PCR method could be used as an ideal... Objective To find out an ideal method used in genotypic identification of Streptococcus mutans by arbitrarily primed polymerase chain reaction finger printing (AP PCR). Method The Streptococcus mutans standard strains were used with different polymerase chain reaction (PCR) conditions on general primer (5′ TGCCGAGCTG 3′) to select an ideal result. Result An ideal PCR reaction was found:94 ℃ 30 s,36 ℃ 30 s,72 ℃ 1 min,45 cycles. Conclusion AP PCR method could be used as an ideal method for Streptococcus mutans strains' genotypic identification. :【目的】探索一种利用随机引物聚合酶链反应指纹法技术进行变形链球菌基因分型的方法。【方法】利用变形链球菌不同血清型标准株和通用引物 5′ TGCCGAGCTG 3′,研究了在不同条件下进行聚合酶链反应的结果 ,寻找满意的聚合酶链反应条件。【结果】找到了较为满意的聚合酶链反应条件 :94℃ 30s,36℃ 30s,72℃ 1min ,反应进行 45个循环。【结论】随机引物聚合酶链反应指纹法技术在一定的控制条件下可用于变形链球菌的鉴定和分型 WT5”BZ]Objective To study Streptococcus mutans acid production inhibition by methylene blue, and investigate the practicability of methylene blue as new kind of dental caries prevention agent. Methods The kind and quantities of acid produced by Streptococcus mutans in three different culture fluids, NS, 0.5M glucose, 0.5M glucose+0.025%(g/L) methylene blue, were measured with gas chromatography. Results There were significant difference between the total quantities of acid in three different... WT5”BZ]Objective To study Streptococcus mutans acid production inhibition by methylene blue, and investigate the practicability of methylene blue as new kind of dental caries prevention agent. Methods The kind and quantities of acid produced by Streptococcus mutans in three different culture fluids, NS, 0.5M glucose, 0.5M glucose+0.025%(g/L) methylene blue, were measured with gas chromatography. Results There were significant difference between the total quantities of acid in three different culture fluids, their were respectively glucose+methylene blue: 14.66±5.42mmol/L, NS:26.00±5.73mmol/L, glucose: 42.99±7.86mmol/L. However, the kinds of acid in three ctuture fluids were same, in which lactic acid was the greatest among all acids, and methylacetic acid, butyric acid were not found. Conclusion methylene blue has acid production inhibition to Streptococcus mutans, and it is worth kind of dental caries prevention agent. [WT5”HZ] 目的 研究美兰对变形链球菌在外源性糖培养条件下产酸的抑制作用 ,探讨美兰作为一种新型防龋制剂的可行性。方法 通过将美兰加入变形链球菌糖培养液的方法 ,以不加美兰者作对照 ,用气相色谱法检测不同培养条件下变形链球菌培养液中产生有机酸的种类和数量。结果 美兰组有机酸的总量为 14.6 6± 5 .42 mmol/L,与对照组比较相差显著 (P<0 .0 1) ;其中以乳酸的量为最高 ,且均未检出丙酸和丁酸。结论 美兰具有抑制变形链球菌产酸的作用 :美兰作为防龋制剂具有一定的应用价值
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