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genotype
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  genotype
Finally, it is feasible to detect DNA genotype through FISH.
      
hydrophila strains were aerA+hlyA+ahpA+ genotype, and all aerA+hlyA+ahpA+ strains were virulent.
      
Strains with the genotype of aerA-hlyA-ahpA+ have middle pathogenicity.
      
Most of the observed genotype frequencies were found to significantly deviate from the Hardy-Weinberg expectations in both species.
      
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique was used to genotype GNB3 C825T polymorphism in 354 hypertensive (HT) and 384 normotensive (NT) Uygur subjects.
      
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siap reverse transcription nested polymerase chain reaction and restriction fragmentlength polymorphsim, genotypic investigation of hepatitis C virus(HCV)in its5’-no-ncoding region was carried out in HCV- infected patients with primary hepatic carcinoma(PHC,n=33) ,liver cirrhosis(LC,n=18), hepatitis( chronic active=4, chronic per-sistent =2,acute =3, Severe=1)and blood donors(n=.The results showed that allthese 63 individuals were infected with type II HCV,but not type III/IV HCV。The data sugg est that the type...

siap reverse transcription nested polymerase chain reaction and restriction fragmentlength polymorphsim, genotypic investigation of hepatitis C virus(HCV)in its5’-no-ncoding region was carried out in HCV- infected patients with primary hepatic carcinoma(PHC,n=33) ,liver cirrhosis(LC,n=18), hepatitis( chronic active=4, chronic per-sistent =2,acute =3, Severe=1)and blood donors(n=.The results showed that allthese 63 individuals were infected with type II HCV,but not type III/IV HCV。The data sugg est that the type II HCV appear to play a main role in the developmentof HCV-associated PHC and LC and predominant genotype of HCV in hepatitis C alsobelong to the type II in Chongqing area.

应用逆转录-套式聚合酶链反应和限制性片段长度多态性分析方法,对丙型肝炎病毒(HCV)感染的33例原发性肝癌、18例肝硬化、10例肝炎(慢性活动性4例,慢性迁延性2例,急性3例,重型肝炎1例)患者及2例献血员进行HCV5`-末端非编码区基因分型。结果表明此63例所感染的HCV均属于Ⅱ型病毒,未检出Ⅲ/Ⅳ型病毒。提示Ⅱ型HCV感染在重庆地区HCV相关性肝癌和肝硬化的发生中可能起着主导作用,本地区丙型肝炎患者中的流行毒株亦属于Ⅱ型病毒。

6 patients with chronic hepatitis C were received IFN-α1 therapy. The relation of HCV genotype and HCV amount to IFN effects were studied. The result of geno-typing of 20 patients showed : In patients with genotype Ⅰ,Ⅰ and ⅠⅠⅠ HCV, grade A virus elumination(virus completely erdlicated)response was noted in 4 of 6 and 5 of 7 cases respectivel.It indicates that genotype Ⅰ and ⅠⅠⅠ HCV Were easier to be elimi-nated than that of genotype ⅠⅠ(P<0. 05).No evident relation was observed between...

6 patients with chronic hepatitis C were received IFN-α1 therapy. The relation of HCV genotype and HCV amount to IFN effects were studied. The result of geno-typing of 20 patients showed : In patients with genotype Ⅰ,Ⅰ and ⅠⅠⅠ HCV, grade A virus elumination(virus completely erdlicated)response was noted in 4 of 6 and 5 of 7 cases respectivel.It indicates that genotype Ⅰ and ⅠⅠⅠ HCV Were easier to be elimi-nated than that of genotype ⅠⅠ(P<0. 05).No evident relation was observed between genotype and ALT levels. The result of HCV RNA semiquantition of 26 patients sho- wed: There was a negative correlation between serum HCV amount and virus elimina- tion respones(P<0.05),but there was no evident relation between HCV amount and ALT levels. The HCV genotype and serum HCV amount can be the useful reference index of the curative effects of IFN, and it is important to determine the serum HCV RNA in evalution of IFN effects.

用α1型基因工程干扰素(IFN-α1)治疗26例慢性丙型肝炎(丙肝),观察丙型肝炎病毒(HCV)基因型和HCVRNA量和疗效的关系。其中20例作HCV的基因型,结果表明HCVⅠ型中病毒清除反应属A级(完全清除)为4/6,Ⅱ型为0/6,Ⅲ型为5/7,提示Ⅰ、Ⅲ型较Ⅱ型易于清除病毒(P<0.05);基因型和ALT反应关系不明显。26例作HCVRNA,半定量结果表明病毒含量低者,病毒清除反应佳(P<0.05),而和ALT关系不明显。作者认为HCV基因型和血清病毒量可作为预测干扰素疗效的参考指标,定量检测血清中HCVRNA在评价IFN疗效中十分重要。

o explore the characteristics of hepatitis C in China,one hundred and sixty three cases of hepatitis C were followed for 8 years,The rates of abnormal alanine aminotransferase(ALT) were 100%,73%,57%,37%and 28% in less than one,1,2,5 and 8 years after the onset respectively. Abnormality of ALT might be persistent or fluctuating,and the latter type accounted for about 1/3 to 1/2 of the cases. The positive rates of anti-hepatitis C virus(HCV)were 56%, 93%, 94%, 96%, 97%,93%and 83%in less than two,2~6,7~12 months...

o explore the characteristics of hepatitis C in China,one hundred and sixty three cases of hepatitis C were followed for 8 years,The rates of abnormal alanine aminotransferase(ALT) were 100%,73%,57%,37%and 28% in less than one,1,2,5 and 8 years after the onset respectively. Abnormality of ALT might be persistent or fluctuating,and the latter type accounted for about 1/3 to 1/2 of the cases. The positive rates of anti-hepatitis C virus(HCV)were 56%, 93%, 94%, 96%, 97%,93%and 83%in less than two,2~6,7~12 months and 1,2,5 and 8 years after the onset respectively. Negative conver-sion of anti-HCV was found only in a few patients whose ALT had returned to normal level and the rates of negative conversion for them were 10%,8%, 16%,and 22% in 1,2, 5 and 8 years after the onset. Positive rate of HCV-RNA,detected with nested PCR 8 years after the onset was 84%for patients who still showed positive anti-HCV and there was no significant difference between patients with abnormal(80%)and normal ALT (85%)levels, Genotype Ⅱ accounted for 93%of the cases. No patient developed heptocellular carcinoma or decompensated cirrhosis after a follow-up of eight years.

为探讨我国丙型肝炎的特点,对163例丙型肝炎患者进行了8年随访。急性丙型肝炎急性期后的主要异常表现为血清丙氨酸转氮酶(ALT)升高,病后1年内、1、2、5和8年的ALT异常率分别为100%、73%、57%、37%和28%;ALT异常分持续性和波动性两类,后者约占1/3~1/2。用第二代丙型肝炎病毒抗体(抗-HCV)ELISA试剂检测,病后2个月内、2~6个月、7~12个月及1、2、5和8年的抗-HCV阳性率分别为56%、93%、94%、96%、97%、93%和83%;病后1年起少数患者抗-HCV阴转,仅见于ALT复常者,病后1、2、5和8年,ALT复常者的抗-HCV阴转率分别为10%、8%、16%和22%。病后8年时用套式聚合酶链反应(PCR)法检测抗-HCV阳性血,丙型肝炎病毒(HCV)RNA阳性率为84%,其中ALT异常和正常者无明显差异。用型特异引物PCR法测定45份抗-HCV阳性血清,42份为Ⅱ型,3份待定。8年时未检出甲胎蛋白异常者,肝功能(血清胆红素和白蛋白)96%~97%为HobbsⅠ级,表明还没有明显的失代偿性肝硬化和肝细胞癌者。

 
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