The main results shown as follows:1. The genetic variations of the natural and cultured stocks of Paralichthys olivaceus were assessed at 10 microsatellite loci (Pol, Po13, Po33, Po35, Po42, Po48, Po56, Po89, Po91, Po-strl). All loci are polymorphism.
Five special PCR primers for Glu-A1x, Glu-B1x, Glu-A3, Glu-B3 and Glu-1Dx5, and 2 SSR primers for γ-gliadin and LMW-glutenin were used to investigate the genetic variations of storage protein genes among 40 Chinese endemic wheat accessions, including 8 Sichuan white wheat landraces, 14 Yunnan hulled wheat accessions, 9 Tibetan weedrace and 9 Xinjiang rice wheat accessions.
110 alleles were examined on 7 microsatellite sites and the average heterozygosity,polymorphism information content and effective number alleles on 7 microsatellite sites are 0.8867,0.8774 and 11.2907.Abundant of genetic diversity was reflected according to high genetic variations,and genetic variations carried out by microsatellite DNA markers is higher than structural loci.
We study the genetic variations by using RAPD and ISSR markers to track 47 samples of wild lotus from Ussuri River, Songhua River and 2 samples from Heilong River Valleys in Russia and 27 samples of cultivated lotus from other provinces in China.
The genetic variations of four ginseng populations (32 individuals) were confirmed by random amplified polymorphic DNA (RAPD) markers and 46.1% polymorphic sites were detected, which proved that the genetic diversity level in the garden ginseng ( Panax ginseng C.A. Meyer) was relatively high.
The genetic variations and heterozygosities of 8 chicken breeds (or lines) and 2 hybrid chicken populations were analyzed by using 20 RAPD primers in this study,UPGMA cluster analysis based on the 10 chicken populations was performed applying the genetic distances.
The results of RAPD analysis can be considered as an important basis for deciding the origin region,genetic variations,and relationship of the various germplasm materials of “KUDINGCHA” in Ilex L.,and for the classification on the species-level or infraspecific level.
The results showed that a little genetic variations were found between these strains.
By using RAPD and SSR markers, the genetic variations between 98-1266 and Chuanmai 28 were detected. 98-1266 is a new 1BL/1RS translocation line conferring excellent quality, resistance to powdery mildew and stripe rust, and high yield.
The Shannon's Information index and GST were 0.3306 and 0.3263, respectively, indicating that there were more genetic variations within the subpopulations than those among the subpopulations.
To determine the distribution of genetic variations in apolipoprotein E (apoE) and apolipoprotein A-IV (apoA-IV) genes, 137 Russians living in Estonia was screened by isoelectric focusing and immunoblotting procedures.
Allozyme electrophoresis was used to study the genetic variations to complement the morphological features studied that included shell lengths, numbers of whorls and shell colour.
The results showed that the giant panda has low genetic variations, and that there is no notable genetic isolation among geographical populations.
The probing of the genetic variations and the utilition of alloplasmic wheat was proven to be valuable for both genetic study and breeding of new varieties.