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mottle
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  斑驳
    Identification of Carnation Mottle Virus on Carnation in Kunming
    昆明香石竹斑驳病毒鉴定
短句来源
    Sequence Analysis of the CP Gene and Its 3′ Noncoding Region of a Watermelon Liaoning Isolate of Cucumber Green Mottle Mosaic Virus
    黄瓜绿斑驳花叶病毒辽宁分离物外壳蛋白基因与3′非编码区的序列分析
短句来源
    Therefore it was identified to be a strain of SMV.Lify symptomless virus (LSV) and lily mottle virus(LMoV) were detected in the diseased lily samples from Europe Sorbonne ( HZ2) by RT-PCR with genera-specific degenerate primers of Polyvius and Carlavirus. , whereas only LSV was detected in musk lily of Hangzhou (L longirlorum) and introduced lily from Europe Sorbonne (HZ4) .
    用特异性科属简并引物检测患病百合的线状植物病毒,在引进的欧洲百合品种Sorbonne(HZ2)中存在百合无症病毒(Lily symptomless virus,LSV)和百合斑驳病毒(Lily mottle virus,LMoV)的复合侵染,杭州麝香百合(L.Longirlorum)和引进的欧洲百合品种Siberia(HZ4)中仅含有LSV。
短句来源
    Carnation virus diseases in major carnation producing region in Fujian had been investigated in 1995-1998 The results of symptomatology,biological identification,electron microscopy and indrect ELISA test showed that carnation mottle virus(CaMV) was a main epidemic virus in carnation with a virua\|carring rate of 30 8%-72 4% CaMV elimination had been studied.
    1995~1998 年调查了福建省香石竹主要种植地的病毒病, 经症状观察、生物学鉴定、电镜观察和间接 E L I S A 法检测, 明确了香石竹斑驳病毒 ( Ca M V) 是福建省田间流行的主要病毒种类, 带毒率为308% ~724% 。
短句来源
    Identification and elimination of carnation mottle virus in Fujian,China
    福建省香石竹斑驳病毒的鉴定及其脱除
短句来源
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  “mottle”译为未确定词的双语例句
    DELETION ANALYSIS AND FUNCTIONAL STUDIES OF THE PROMOTER FROM COMMELINA YELLOW MOTTLE VIRUS
    竹节花黄斑驳病毒启动子的缺失分析及功能
短句来源
    amaranticolor. The virus caused chlorotic local lesions on inoculated bean (Phaseolus vulgaris cv. Bountiful) leaves, and mottle, vein clearing and distortion on systemically leaves.
    在菜豆(Phaseolus valgaris cv.Bountiful)呈系统症状,三生小叶花叶,扭曲畸形。
短句来源
  相似匹配句对
    Identification of Carnation Mottle Virus on Carnation in Kunming
    昆明香石竹斑驳病毒鉴定
短句来源
    Cloning and sequencing of Ryegrass mottle virus genome
    多花黑麦草斑驳病毒基因组克隆与序列分析
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  mottle
Detection and characterization of defective interfering RNAs associated with the cocksfoot mottle sobemovirus
      
A new RNA of about 900 nt was found in the virions of cocksfoot mottle virus (CfMV) and in infected plants by RNA hybridization and RT-PCR.
      
Specifics of the coat protein gene in Russian strains of the cucumber green mottle mosaic virus
      
The primary structure of the coat protein (CP) gene was examined for pathogenic strain MS-1 and vaccine strain VIROG-43M of the cucumber green mottle mosaic virus (CGMMV).
      
The results of X-ray diffraction studies of a number of proteins and the carnation mottle virus performed over a period from 1970 to 2000 at the laboratory created by Academician B.K.
      
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Tested negative stain,ultrathin section and Immuno Electron Microscopy(IEM)etc.that methods of preparation specimen of Electron Microscope(EM)by usingthe following materials:healty tissue of tobacco leaf,tissue of tobacco leaf infectedAlternaria alternata,tissue of maize lacked element,and the pathogen of plant virusessuch as Tobacco Mosaic Virus(TMV),Cucuinber Mosaic Virus(CMV),Potato VirusY(PVY),Potato Virus X(PVX),Carnation Mottle Virus(CarMV ect.,and thepathogenic bacteria of plant sucli as Xanthomonas...

Tested negative stain,ultrathin section and Immuno Electron Microscopy(IEM)etc.that methods of preparation specimen of Electron Microscope(EM)by usingthe following materials:healty tissue of tobacco leaf,tissue of tobacco leaf infectedAlternaria alternata,tissue of maize lacked element,and the pathogen of plant virusessuch as Tobacco Mosaic Virus(TMV),Cucuinber Mosaic Virus(CMV),Potato VirusY(PVY),Potato Virus X(PVX),Carnation Mottle Virus(CarMV ect.,and thepathogenic bacteria of plant sucli as Xanthomonas Oryzae,the pathogenic fungus of plantsuch as Pirycularia Orvzae etc.,found out the working condition of the preparation spec-imen of EM for aforementioned plant and plant pathogen,and it has simplified and im-proved in comparison with the methods of the literature reported.

应用健康烟叶组织,感染赤星病的烟叶组织.玉米缺素组织以及植物病原病毒烟草花叶病毒(TMV)、黄瓜花叶病毒(CMV)、马铃薯病毒Y(PVY)、马铃薯病毒X(PVX)、香石竹班驳病毒(CarMV)等和植物病原细菌XanthomonasOryzae.病原真菌PiryculariaOryzde等材料,试验了超薄切片法、负染法及免疫电镜等方法制样观察,探索出上述植物及其病原的电镜样品制备的工作条件,与文献报道的方法相比略有简化和改进.

Commelina Yellow Mottle Virus(CoYMV) is a double stranded, circular DNA virus and its promoter could direct GUS gene specifically expressing in phloem tissue of transgenic tobacco plants. To determine the optimal promoter sequence for phloem specific gene expression, CoYMV promoter was deleted from its 5′ end to form promoter fragments with 5 different lengths. Chimeric GUS genes were constructed using the promoter deletion based on the binary vector pBI121. Transgenic tobacco plants evidenced by PCR...

Commelina Yellow Mottle Virus(CoYMV) is a double stranded, circular DNA virus and its promoter could direct GUS gene specifically expressing in phloem tissue of transgenic tobacco plants. To determine the optimal promoter sequence for phloem specific gene expression, CoYMV promoter was deleted from its 5′ end to form promoter fragments with 5 different lengths. Chimeric GUS genes were constructed using the promoter deletion based on the binary vector pBI121. Transgenic tobacco plants evidenced by PCR analysis were obtained with each kind of chimeric GUS gene structure by Agrobacterium mediated transformation. The results of GUS activity assay and histochemical staining showed that most of the chimeric GUS genes were expressed in transgenic plants. The GUS activity with the promoter deleted to -870bp was about 78% higher than that of the full length promoter(1040bp) and was a little higher than that of the promoter deleted to -585bp, but the difference is not significant. The GUS activity reduced significantly when the promoter was deleted to -447bp or -232bp, whereas the property of phloem specific expression pattern was still retained. When the promoter was deleted to -44bp, just upstream adjacent to the TATA box, its tissue specificity was lost and the activity was reduced to undetectable level.These results suggest that the region between -870bp~232bp and downstream of -232bp of CoYMV promoter could be responsible for promoter activity and tissue specific expression, respectively. A negative regulation sequence might exist upstream of -870bp of the CoYMV promoter. Therefore, we recommend that the optional CoYMV promoter sequence for phloem specific expression could be downstream from -870bp or -585bp. In comparison with CaMV 35S promoter, the GUS activity when driven by -870bp CoYMV promoter was about 70% of that when driven by the 35S promoter. Considering the fact that 35S promoter_GUS gene is constitutively expressed, while the CoYMV promoter GUS gene is expressed only in phloem tissues, the activity of the latter in phloem may be the same with or even higher than that of the 35S promoter.

竹节花黄斑驳病毒(CoYMV)是侵染单子叶植物竹节花的一种双链环状DNA病毒,它的启动子可介导外源基因在烟草韧皮部特异表达。为了研究其组织特异性表达的最佳启动子区域,对CoYMV启动子进行了5′端五种不同长度的缺失分析,用不同长度的启动子片段与GUS基因及NOS3′端转录中止序列构建了全长启动子及5个缺失启动子序列的六个嵌合GUS基因植物表达载体。利用农杆菌将上述嵌合基因转化烟草外植体后,每种表达载体都获得了一批转基因烟草植株。转化再生烟草植株的PCR分析、GUS酶活测定及GUS组织染色的结果表明六种类型的嵌合基因已整合到烟草染色体中,并有五种表达出GUS活性。缺失到870bp的启动子比全长启动子(1040bp)的活性约高78%,870bp比585bp启动子介导的GUS活性略高但差别不明显,缺失到447和232时GUS活性有明显下降,但仍具有韧皮部特异表达的特性。当缺失到TATAbox附近的44bp时启动子丧失组织特异性,GUS活性也降低到测不出来的水平。以上结果表明CoYMV启动子从转录起始位点上游870bp~230bp及232bp下游区分别与启动子的活性和韧皮部组织特异性密切相关,87?

Carnation virus diseases in major carnation producing region in Fujian had been investigated in 1995-1998 The results of symptomatology,biological identification,electron microscopy and indrect ELISA test showed that carnation mottle virus(CaMV) was a main epidemic virus in carnation with a virua\|carring rate of 30 8%-72 4% CaMV elimination had been studied.Both heating treatment and shoot tip culture were not effective method for CaMV elimination The elimination efficiencies of CaMV are 35 7%、46...

Carnation virus diseases in major carnation producing region in Fujian had been investigated in 1995-1998 The results of symptomatology,biological identification,electron microscopy and indrect ELISA test showed that carnation mottle virus(CaMV) was a main epidemic virus in carnation with a virua\|carring rate of 30 8%-72 4% CaMV elimination had been studied.Both heating treatment and shoot tip culture were not effective method for CaMV elimination The elimination efficiencies of CaMV are 35 7%、46 7%、61 5% respectively,when using a combination of shoot tip culture with treatment of medication i e containing 2 ml·L -1 ,5 ml·L -1 ,10 ml·L -1 of Bingdubike compound in medium The experiments also showed that,low concentration of Bingdubike(2-5 ml·L -1 ) promoted the growth of shoot\|tips,whereas,more high concentration(>10 ml·L -1 ) had an obvious harmness to the plant growth.

1995~1998 年调查了福建省香石竹主要种植地的病毒病, 经症状观察、生物学鉴定、电镜观察和间接 E L I S A 法检测, 明确了香石竹斑驳病毒 ( Ca M V) 是福建省田间流行的主要病毒种类, 带毒率为308% ~724% 。脱毒技术的研究结果表明, 热处理、茎尖培养脱毒效果均不够理想, 而茎尖培养加药物处理, 在培养基中加病毒必克2 m l· L- 1、5 m l· L- 1、10 m l· L- 1, Ca M V脱除率分别达 357% 、467% 、615% ; 低浓度 (2~5 m l· L- 1) 对茎尖生长有促进作用, 较高浓度 (> 10 m l· L- 1) 对植株生长有明显毒害作用。

 
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